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Protein

Envelope glycoprotein H

Gene

gH

Organism
Epstein-Barr virus (strain B95-8) (HHV-4) (Human herpesvirus 4)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

The heterodimer glycoprotein H-glycoprotein L is required for the fusion of viral and plasma membranes leading to virus entry into the host cell. Following initial binding to host receptor, membrane fusion is mediated by the fusion machinery composed of gB and the heterodimer gH/gL. May also be involved in the fusion between the virion envelope and the outer nuclear membrane during virion morphogenesis.UniRule annotation1 Publication

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Fusion of virus membrane with host cell membrane, Fusion of virus membrane with host membrane, Viral penetration into host cytoplasm, Virus entry into host cell

Keywords - Ligandi

Sialic acid

Names & Taxonomyi

Protein namesi
Recommended name:
Envelope glycoprotein HUniRule annotation
Short name:
gHUniRule annotation
Gene namesi
Name:gHUniRule annotation
ORF Names:BXLF2
OrganismiEpstein-Barr virus (strain B95-8) (HHV-4) (Human herpesvirus 4)
Taxonomic identifieri10377 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeGammaherpesvirinaeLymphocryptovirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000007640 Componenti: Genome

Subcellular locationi

  • Virion membrane UniRule annotation; Single-pass type I membrane protein UniRule annotation
  • Host cell membrane UniRule annotation; Single-pass type I membrane protein UniRule annotation
  • Host endosome membrane UniRule annotation; Single-pass type I membrane protein UniRule annotation

  • Note: During virion morphogenesis, this protein probably accumulates in the endosomes and trans-Golgi where secondary envelopment occurs. It is probably transported to the cell surface from where it is endocytosed and directed to the trans-Golgi network (TGN).UniRule annotation

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini19 – 682664Virion surfaceUniRule annotationAdd
BLAST
Transmembranei683 – 70321HelicalUniRule annotationAdd
BLAST
Topological domaini704 – 7063IntravirionUniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host cell membrane, Host endosome, Host membrane, Membrane, Viral envelope protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818UniRule annotationAdd
BLAST
Chaini19 – 706688Envelope glycoprotein HUniRule annotationPRO_0000436649Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi60 – 601N-linked (GlcNAc...); by hostUniRule annotation
Glycosylationi435 – 4351N-linked (GlcNAc...); by hostUniRule annotation
Glycosylationi549 – 5491N-linked (GlcNAc...); by hostUniRule annotation
Glycosylationi604 – 6041N-linked (GlcNAc...); by hostUniRule annotation
Glycosylationi664 – 6641N-linked (GlcNAc...); by hostUniRule annotation

Post-translational modificationi

N-glycosylated, O-glycosylated, and sialylated.UniRule annotation

Keywords - PTMi

Glycoprotein

Interactioni

Subunit structurei

Interacts with glycoprotein L (gL); this interaction is necessary for the correct processing and cell surface expression of gH. The heterodimer gH/gL seems to interact with gB trimers during fusion.UniRule annotation

Protein-protein interaction databases

DIPiDIP-49016N.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3PHFX-ray3.581/3/5/A/C/E/G/I/K/M/O/Q/S/U/W/Y20-672[»]
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP03231.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni165 – 22965Interaction with gLUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the herpesviridae glycoprotein H family.UniRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

KOiK19451.

Family and domain databases

HAMAPiMF_04033. HSV_GH. 1 hit.
InterProiIPR003493. Herpes_gH.
[Graphical view]
PfamiPF02489. Herpes_glycop_H. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P03231-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQLLCVFCLV LLWEVGAASL SEVKLHLDIE GHASHYTIPW TELMAKVPGL
60 70 80 90 100
SPEALWREAN VTEDLASMLN RYKLIYKTSG TLGIALAEPV DIPAVSEGSM
110 120 130 140 150
QVDASKVHPG VISGLNSPAC MLSAPLEKQL FYYIGTMLPN TRPHSYVFYQ
160 170 180 190 200
LRCHLSYVAL SINGDKFQYT GAMTSKFLMG TYKRVTEKGD EHVLSLVFGK
210 220 230 240 250
TKDLPDLRGP FSYPSLTSAQ SGDYSLVIVT TFVHYANFHN YFVPNLKDMF
260 270 280 290 300
SRAVTMTAAS YARYVLQKLV LLEMKGGCRE PELDTETLTT MFEVSVAFFK
310 320 330 340 350
VGHAVGETGN GCVDLRWLAK SFFELTVLKD IIGICYGATV KGMQSYGLER
360 370 380 390 400
LAAMLMATVK MEELGHLTTE KQEYALRLAT VGYPKAGVYS GLIGGATSVL
410 420 430 440 450
LSAYNRHPLF QPLHTVMRET LFIGSHVVLR ELRLNVTTQG PNLALYQLLS
460 470 480 490 500
TALCSALEIG EVLRGLALGT ESGLFSPCYL SLRFDLTRDK LLSMAPQEAT
510 520 530 540 550
LDQAAVSNAV DGFLGRLSLE REDRDAWHLP AYKCVDRLDK VLMIIPLINV
560 570 580 590 600
TFIISSDREV RGSALYEAST TYLSSSLFLS PVIMNKCSQG AVAGEPRQIP
610 620 630 640 650
KIQNFTRTQK SCIFCGFALL SYDEKEGLET TTYITSQEVQ NSILSSNYFD
660 670 680 690 700
FDNLHVHYLL LTTNGTVMEI AGLYEERAHV VLAIILYFIA FALGIFLVHK

IVMFFL
Length:706
Mass (Da):78,322
Last modified:July 21, 1986 - v1
Checksum:iA1006BDA6633B1D9
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti380 – 38910TVGYPKAGVY → GAPGGRAGPP in CAE82041 (PubMed:2835748).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01555 Genomic DNA. Translation: CAA24797.1.
AJ507799 Genomic DNA. Translation: CAD53450.1.
X07532 Genomic DNA. Translation: CAE82041.1.
PIRiA93065. QQBE6L.
S00736.
RefSeqiYP_401700.1. NC_007605.1.

Genome annotation databases

GeneIDi3783742.
KEGGivg:3783742.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01555 Genomic DNA. Translation: CAA24797.1.
AJ507799 Genomic DNA. Translation: CAD53450.1.
X07532 Genomic DNA. Translation: CAE82041.1.
PIRiA93065. QQBE6L.
S00736.
RefSeqiYP_401700.1. NC_007605.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3PHFX-ray3.581/3/5/A/C/E/G/I/K/M/O/Q/S/U/W/Y20-672[»]
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-49016N.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi3783742.
KEGGivg:3783742.

Phylogenomic databases

KOiK19451.

Miscellaneous databases

EvolutionaryTraceiP03231.

Family and domain databases

HAMAPiMF_04033. HSV_GH. 1 hit.
InterProiIPR003493. Herpes_gH.
[Graphical view]
PfamiPF02489. Herpes_glycop_H. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGH_EBVB9
AccessioniPrimary (citable) accession number: P03231
Secondary accession number(s): Q6LBU6, Q777C0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: September 7, 2016
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.