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Protein

Secreted protein BARF1

Gene

BARF1

Organism
Epstein-Barr virus (strain B95-8) (HHV-4) (Human herpesvirus 4)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Plays diverse functions in immunomodulation and oncogenicity, maybe by acting as a functional receptor for human CSF1. May inhibit interferon secretion from mononuclear cells. Exhibits oncogenic activity in vitro.3 Publications

Names & Taxonomyi

Protein namesi
Recommended name:
Secreted protein BARF1
Alternative name(s):
33 kDa early protein
p33
Gene namesi
ORF Names:BARF1
OrganismiEpstein-Barr virus (strain B95-8) (HHV-4) (Human herpesvirus 4)
Taxonomic identifieri10377 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeGammaherpesvirinaeLymphocryptovirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000007640 Componenti: Genome

Subcellular locationi

  • Secreted 1 Publication

  • Note: Massively secreted in the serum of EBV-induced nasophyryngeal carcinoma patients.

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Keywords - Diseasei

Oncogene

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
ChainiPRO_000040839121 – 221Secreted protein BARF1Add BLAST201

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi95N-linked (GlcNAc...); by host1 Publication1
Disulfide bondi146 ↔ 2011 Publication

Post-translational modificationi

Phosphorylated on serine and threonine by host.

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiP03228.

Expressioni

Keywords - Developmental stagei

Early protein

Interactioni

Subunit structurei

Homohexamer. Interacts with human CSF1.1 Publication

Protein-protein interaction databases

DIPiDIP-47684N.
MINTiMINT-6768244.

Structurei

Secondary structure

1221
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi22 – 24Combined sources3
Beta strandi29 – 34Combined sources6
Helixi41 – 43Combined sources3
Beta strandi44 – 51Combined sources8
Helixi53 – 55Combined sources3
Beta strandi57 – 64Combined sources8
Beta strandi67 – 70Combined sources4
Helixi72 – 74Combined sources3
Beta strandi78 – 83Combined sources6
Beta strandi86 – 93Combined sources8
Helixi96 – 98Combined sources3
Beta strandi100 – 108Combined sources9
Beta strandi111 – 133Combined sources23
Beta strandi142 – 152Combined sources11
Beta strandi154 – 159Combined sources6
Beta strandi179 – 181Combined sources3
Beta strandi183 – 191Combined sources9
Beta strandi198 – 205Combined sources8
Beta strandi208 – 216Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2CH8X-ray2.30A/B/C/D21-221[»]
3UEZX-ray3.41A/B/C/D21-221[»]
4ADFX-ray4.40A/B/C/D/E/F/M/N/O/P/Q/R21-221[»]
4ADQX-ray4.50A/B/C/D21-221[»]
ProteinModelPortaliP03228.
SMRiP03228.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP03228.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini21 – 120Ig-like 1Add BLAST100
Domaini124 – 220Ig-like 2Add BLAST97

Sequence similaritiesi

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 2 hits.
PROSITEiPS50835. IG_LIKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P03228-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARFIAQLLL LASCVAAGQA VTAFLGERVT LTSYWRRVSL GPEIEVSWFK
60 70 80 90 100
LGPGEEQVLI GRMHHDVIFI EWPFRGFFDI HRSANTFFLV VTAANISHDG
110 120 130 140 150
NYLCRMKLGE TEVTKQEHLS VVKPLTLSVH SERSQFPDFS VLTVTCTVNA
160 170 180 190 200
FPHPHVQWLM PEGVEPAPTA ANGGVMKEKD GSLSVAVDLS LPKPWHLPVT
210 220
CVGKNDKEEA HGVYVSGYLS Q
Length:221
Mass (Da):24,471
Last modified:July 21, 1986 - v1
Checksum:iCA5A24D1EA28758E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01555 Genomic DNA. Translation: CAA24809.1.
AJ507799 Genomic DNA. Translation: CAD53469.1.
PIRiB43045. QQBE48.
RefSeqiYP_401719.1. NC_007605.1.

Genome annotation databases

GeneIDi3783772.
KEGGivg:3783772.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01555 Genomic DNA. Translation: CAA24809.1.
AJ507799 Genomic DNA. Translation: CAD53469.1.
PIRiB43045. QQBE48.
RefSeqiYP_401719.1. NC_007605.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2CH8X-ray2.30A/B/C/D21-221[»]
3UEZX-ray3.41A/B/C/D21-221[»]
4ADFX-ray4.40A/B/C/D/E/F/M/N/O/P/Q/R21-221[»]
4ADQX-ray4.50A/B/C/D21-221[»]
ProteinModelPortaliP03228.
SMRiP03228.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-47684N.
MINTiMINT-6768244.

Proteomic databases

PRIDEiP03228.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi3783772.
KEGGivg:3783772.

Miscellaneous databases

EvolutionaryTraceiP03228.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 2 hits.
PROSITEiPS50835. IG_LIKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBARF1_EBVB9
AccessioniPrimary (citable) accession number: P03228
Secondary accession number(s): Q777A5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: November 30, 2016
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.