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Protein

Tripartite terminase subunit 3

Gene

TRM3

Organism
Epstein-Barr virus (strain B95-8) (HHV-4) (Human herpesvirus 4)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Component of the molecular motor that translocates viral genomic DNA in empty capsid during DNA packaging. Forms a tripartite terminase complex together with TRM1 and TRM2 in the host cytoplasm. Once the complex reaches the host nucleus, it interacts with the capsid portal vertex. This portal forms a ring in which genomic DNA is translocated into the capsid. TRM3 carries an RNase H-like nuclease activity that plays an important role for the cleavage of concatemeric viral DNA into unit length genomes.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei326For ATPase activityUniRule annotation1
Active sitei481For nuclease activityUniRule annotation1
Active sitei555For nuclease activityUniRule annotation1
Active sitei667For nuclease activityUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Viral genome packaging, Virus exit from host cell

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Tripartite terminase subunit 3UniRule annotation (EC:3.1.-.-UniRule annotation)
Alternative name(s):
Terminase large subunitUniRule annotation
Gene namesi
Name:TRM3UniRule annotation
ORF Names:BGRF1/BDRF1
OrganismiEpstein-Barr virus (strain B95-8) (HHV-4) (Human herpesvirus 4)
Taxonomic identifieri10377 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeGammaherpesvirinaeLymphocryptovirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000007640 Componenti: Genome

Subcellular locationi

  • Host nucleus UniRule annotation

  • Note: Responsible for the nuclear localization of the two others subunits TRM1 and TRM2.UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001159461 – 690Tripartite terminase subunit 3Add BLAST690

Interactioni

Subunit structurei

Interacts with the terminase subunits TRM1 and TRM2. Interacts with portal protein.UniRule annotation

Binary interactionsi

WithEntry#Exp.IntActNotes
BTRF1Q1HVD33EBI-9644804,EBI-9644838From a different organism.

Protein-protein interaction databases

IntActiP03219. 26 interactors.
MINTiMINT-6768148.

Structurei

3D structure databases

ProteinModelPortaliP03219.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi226 – 233Walker A motifUniRule annotation8
Motifi321 – 326Walker B motifUniRule annotation6

Sequence similaritiesi

Belongs to the herpesviridae TRM3 protein family.UniRule annotation

Family and domain databases

HAMAPiMF_04013. HSV_TRM3. 1 hit.
InterProiIPR003498. DNA_pack_C.
IPR003499. DNA_pack_N.
IPR033663. HSV_TRM3.
[Graphical view]
PfamiPF02499. DNA_pack_C. 1 hit.
PF02500. DNA_pack_N. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P03219-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLYASQRGRL TENLRNALQQ DSTTQGCLGA ETPSIMYTGA KSDRWAHPLV
60 70 80 90 100
GTIHASNLYC PMLRAYCRHY GPRPVFVASD ESLPMFGASP ALHTPVQVQM
110 120 130 140 150
CLLPELRDTL QRLLPPPNLE DSEALTEFKT SVSSARAILE DPNFLEMREF
160 170 180 190 200
VTSLASFLSG QYKHKPARLE AFQKQVVLHS FYFLISIKSL EITDTMFDIF
210 220 230 240 250
QSAFGLEEMT LEKLHIFKQK ASVFLIPRRH GKTWIVVAII SLILSNLSNV
260 270 280 290 300
QIGYVAHQKH VASAVFTEII DTLTKSFDSK RVEVNKETST ITFRHSGKIS
310 320 330 340 350
STVMCATCFN KNSIRGQTFH LLFVDEANFI KKEALPAILG FMLQKDAKII
360 370 380 390 400
FISSVNSADQ ATSFLYKLKD AQERLLNVVS YVCQEHRQDF DMQDSMVSCP
410 420 430 440 450
CFRLHIPSYI TMDSNIRATT NLFLDGAFST ELMGDTSSLS QGSLSRTVRD
460 470 480 490 500
DAINQLELCR VDTLNPRVAG RLASSLYVYV DPAYTNNTSA SGTGIAAVTH
510 520 530 540 550
DRADPNRVIV LGLEHFFLKD LTGDAALQIA TCVVALVSSI VTLHPHLEEV
560 570 580 590 600
KVAVEGNSSQ DSAVAIASII GESCPLPCAF VHTKDKTSSL QWPMYLLTNE
610 620 630 640 650
KSKAFERLIY AVNTASLSAS QVTVSNTIQL SFDPVLYLIS QIRAIKPIPL
660 670 680 690
RDGTYTYTGK QRNLSDDVLV ALVMAHFLAT TQKHTFKKVH
Length:690
Mass (Da):76,694
Last modified:April 1, 1993 - v2
Checksum:i849EBABCEFF1977C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01555 Genomic DNA. Translation: CAA24829.1. Sequence problems.
V01555 Genomic DNA. Translation: CAA24834.1.
AJ507799 Genomic DNA. Translation: CAD53440.1.
PIRiA43044. QQBE38.
RefSeqiYP_401690.1. NC_007605.1.

Genome annotation databases

GeneIDi17494212.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01555 Genomic DNA. Translation: CAA24829.1. Sequence problems.
V01555 Genomic DNA. Translation: CAA24834.1.
AJ507799 Genomic DNA. Translation: CAD53440.1.
PIRiA43044. QQBE38.
RefSeqiYP_401690.1. NC_007605.1.

3D structure databases

ProteinModelPortaliP03219.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP03219. 26 interactors.
MINTiMINT-6768148.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi17494212.

Family and domain databases

HAMAPiMF_04013. HSV_TRM3. 1 hit.
InterProiIPR003498. DNA_pack_C.
IPR003499. DNA_pack_N.
IPR033663. HSV_TRM3.
[Graphical view]
PfamiPF02499. DNA_pack_C. 1 hit.
PF02500. DNA_pack_N. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRM3_EBVB9
AccessioniPrimary (citable) accession number: P03219
Secondary accession number(s): Q777C9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: April 1, 1993
Last modified: October 5, 2016
This is version 71 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.