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Protein

Epstein-Barr nuclear antigen 1

Gene

EBNA1

Organism
Epstein-Barr virus (strain B95-8) (HHV-4) (Human herpesvirus 4)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays an essential role in replication and partitioning of viral genomic DNA during latent viral infection. During this phase, the circular double-stranded viral DNA undergoes replication once per cell cycle and is efficiently partitioned to the daughter cells. EBNA1 activates the initiation of viral DNA replication through binding to specific sites in the viral latent origin of replication, oriP. Additionally, it governs the segregation of viral episomes by mediating their attachment to host cell metaphase chromosomes. Also activates the transcription of several viral latency genes. Finally, it can counteract the stabilization of host p53/TP53 by host USP7, thereby decreasing apoptosis and increasing host cell survival.1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding
Biological processHost-virus interaction, Inhibition of host adaptive immune response by virus, Inhibition of host NF-kappa-B by virus, Inhibition of host proteasome antigen processing by virus, Transcription, Transcription regulation, Viral immunoevasion, Viral latency, Viral latency initiation and maintenance

Names & Taxonomyi

Protein namesi
Recommended name:
Epstein-Barr nuclear antigen 1
Short name:
EBNA-1
Short name:
EBV nuclear antigen 1
Gene namesi
Name:EBNA1
ORF Names:BKRF1
OrganismiEpstein-Barr virus (strain B95-8) (HHV-4) (Human herpesvirus 4)
Taxonomic identifieri10377 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeGammaherpesvirinaeLymphocryptovirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000153037 Componenti: Genome

Subcellular locationi

  • Host nucleus 1 Publication

GO - Cellular componenti

Keywords - Cellular componenti

Host nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi444E → A: Slight decrease in binding to USP7. Major decrease in binding to USP7; when associated with A-447. 1 Publication1
Mutagenesisi447S → A: Loss of binding to USP7. Major decrease in binding to USP7; when associated with A-444. 1 Publication1

Chemistry databases

ChEMBLiCHEMBL1293281

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001161751 – 641Epstein-Barr nuclear antigen 1Add BLAST641

Interactioni

Subunit structurei

Interacts with human USP7. Interacts with human EBP2; this interaction is important for the stable segregation of EBV episomes during cell division but not for the replication of the episomes. Interacts with BGLF4; this interaction facilitates the switch from latent to lytic DNA replication by down-regulating EBNA1 replication function.4 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi97175112 interactors.
DIPiDIP-29054N
ELMiP03211
IntActiP03211 51 interactors.
MINTiP03211

Chemistry databases

BindingDBiP03211

Structurei

Secondary structure

1641
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi411 – 413Combined sources3
Helixi478 – 489Combined sources12
Beta strandi503 – 512Combined sources10
Helixi514 – 527Combined sources14
Beta strandi537 – 540Combined sources4
Beta strandi545 – 548Combined sources4
Helixi552 – 554Combined sources3
Beta strandi556 – 567Combined sources12
Helixi569 – 583Combined sources15
Helixi590 – 592Combined sources3
Beta strandi593 – 604Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1B3TX-ray2.20A/B461-607[»]
1VHIX-ray2.50A/B466-607[»]
1YY6X-ray1.70B441-450[»]
2FYYX-ray1.50C407-417[»]
2FZ3X-ray1.90C407-417[»]
3MV7X-ray2.00C407-417[»]
3MV8X-ray2.10C407-417[»]
3MV9X-ray2.70C407-417[»]
4PRAX-ray1.85C407-417[»]
4PREX-ray1.65C407-417[»]
4PRIX-ray2.40C407-417[»]
4PRPX-ray2.50C407-417[»]
5WMFX-ray1.90A/B/C/D/E/F470-619[»]
5WUMX-ray2.00B/C378-386[»]
5WUNX-ray2.20B/C378-386[»]
ProteinModelPortaliP03211
SMRiP03211
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP03211

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni436 – 450Interaction with USP7Add BLAST15

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi87 – 352Ala/Gly-richAdd BLAST266

Sequence similaritiesi

Belongs to the herpesviridae EBNA1 family.Curated

Phylogenomic databases

KOiK19461
OrthoDBiVOG090000Z3

Family and domain databases

Gene3Di3.30.70.3901 hit
InterProiView protein in InterPro
IPR035975 E2/EBNA1_C_sf
IPR004186 EBNA1_DNA-bd
IPR037007 EBNA1_DNA-bd_sf
PfamiView protein in Pfam
PF02905 EBV-NA1, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD037044 EBNA1_DNA_bd, 1 hit
SUPFAMiSSF54957 SSF54957, 1 hit

Sequencei

Sequence statusi: Complete.

P03211-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDEGPGTGP GNGLGEKGDT SGPEGSGGSG PQRRGGDNHG RGRGRGRGRG
60 70 80 90 100
GGRPGAPGGS GSGPRHRDGV RRPQKRPSCI GCKGTHGGTG AGAGAGGAGA
110 120 130 140 150
GGAGAGGGAG AGGGAGGAGG AGGAGAGGGA GAGGGAGGAG GAGAGGGAGA
160 170 180 190 200
GGGAGGAGAG GGAGGAGGAG AGGGAGAGGG AGGAGAGGGA GGAGGAGAGG
210 220 230 240 250
GAGAGGAGGA GGAGAGGAGA GGGAGGAGGA GAGGAGAGGA GAGGAGAGGA
260 270 280 290 300
GGAGAGGAGG AGAGGAGGAG AGGGAGGAGA GGGAGGAGAG GAGGAGAGGA
310 320 330 340 350
GGAGAGGAGG AGAGGGAGAG GAGAGGGGRG RGGSGGRGRG GSGGRGRGGS
360 370 380 390 400
GGRRGRGRER ARGGSRERAR GRGRGRGEKR PRSPSSQSSS SGSPPRRPPP
410 420 430 440 450
GRRPFFHPVG EADYFEYHQE GGPDGEPDVP PGAIEQGPAD DPGEGPSTGP
460 470 480 490 500
RGQGDGGRRK KGGWFGKHRG QGGSNPKFEN IAEGLRALLA RSHVERTTDE
510 520 530 540 550
GTWVAGVFVY GGSKTSLYNL RRGTALAIPQ CRLTPLSRLP FGMAPGPGPQ
560 570 580 590 600
PGPLRESIVC YFMVFLQTHI FAEVLKDAIK DLVMTKPAPT CNIRVTVCSF
610 620 630 640
DDGVDLPPWF PPMVEGAAAE GDDGDDGDEG GDGDEGEEGQ E
Length:641
Mass (Da):56,427
Last modified:July 21, 1986 - v1
Checksum:i4D161653E16FC341
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01555 Genomic DNA Translation: CAA24816.1
M13941 Genomic DNA Translation: AAA45889.1
AJ507799 Genomic DNA Translation: CAD53427.1
PIRiC43043 QQBE31
RefSeqiYP_401677.1, NC_007605.1

Genome annotation databases

GeneIDi3783709
KEGGivg:3783709

Similar proteinsi

Entry informationi

Entry nameiEBNA1_EBVB9
AccessioniPrimary (citable) accession number: P03211
Secondary accession number(s): Q777E1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: March 28, 2018
This is version 139 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome