Trans-activator protein BZLF1 - Epstein-Barr virus (strain B95-8) (HHV-4)

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P03206 (BZLF1_EBVB9) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 98. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Trans-activator protein BZLF1
Short name=EB1

Alternative name(s):
Zebra

Gene names

ORF Names:

BZLF1

Organism

Epstein-Barr virus (strain B95-8) (HHV-4) (Human herpesvirus 4) [Reference proteome]

Taxonomic identifier

10377 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Herpesvirales › Herpesviridae › Gammaherpesvirinae › Lymphocryptovirus ›

Virus host

Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	245 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays a key role in the switch from latent infection to lytic cycle producing new virions. Acts as a transcription factor, inducing early lytic cycle genes, and as a origin binding protein for genome replication. BZLF1 activates the promoter of another EBV gene (BSLF2+BMLF1). Ref.4 Ref.5 Ref.6 Ref.9 Ref.11
Subunit structure	Interacts with human UBN1, CRTC2 and GNB2L1/RACK1. Ref.7 Ref.8 Ref.10
Subcellular location	Host nucleus Ref.7.
Sequence similarities	Belongs to the bZIP family. Contains 1 bZIP (basic-leucine zipper) domain.

Ontologies

Keywords
Biological process	G0/G1 host cell cycle checkpoint dysregulation by virus Host-virus interaction Inhibition of host IFN-mediated response initiation by virus Inhibition of host IRF7 by virus Inhibition of host innate immune response by virus Modulation of host cell cycle by virus Transcription Transcription regulation Viral immunoevasion
Cellular component	Host nucleus
Developmental stage	Early protein
Ligand	DNA-binding
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	modulation by virus of host G0/G1 transition checkpoint Inferred from electronic annotation. Source: UniProtKB-KW suppression by virus of host IRF7 activity Inferred from electronic annotation. Source: UniProtKB-KW suppression by virus of host type I interferon production Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	host cell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 245

245

Trans-activator protein BZLF1

PRO_0000076541

Regions

Domain

170 – 228

bZIP

Region

178 – 195

Basic motif By similarity

Region

196 – 228

Leucine-zipper By similarity

Secondary structure

245

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P03206 [UniParc].

Last modified July 1, 1989. Version 2.
Checksum: 7F3D55D79F1F0196
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FASTA

245

26,860

        10         20         30         40         50         60 
MMDPNSTSED VKFTPDPYQV PFVQAFDQAT RVYQDLGGPS QAPLPCVLWP VLPEPLPQGQ 

        70         80         90        100        110        120 
LTAYHVSTAP TGSWFSAPQP APENAYQAYA APQLFPVSDI TQNQQTNQAG GEAPQPGDNS 

       130        140        150        160        170        180 
TVQTAAAVVF ACPGANQGQQ LADIGVPQPA PVAAPARRTR KPQQPESLEE CDSELEIKRY 

       190        200        210        220        230        240 
KNRVASRKCR AKFKQLLQHY REVAAAKSSE NDRLRLLLKQ MCPSLDVDSI IPRTPDVLHE 


DLLNF

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"DNA sequence and expression of the B95-8 Epstein-Barr virus genome." Baer R., Bankier A.T., Biggin M.D., Deininger P.L., Farrell P.J., Gibson T.J., Hatfull G., Hudson G.S., Satchwell S.C., Seguin C., Tuffnell P.S., Barrell B.G. Nature 310:207-211(1984) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"Epstein-Barr virus gene expression in P3HR1-superinfected Raji cells." Biggin M., Bodescot M., Perricaudet M., Farrel P. J. Virol. 61:3120-3132(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA], CHARACTERIZATION.
[3]	"Epstein-Barr virus BZLF1 trans-activator specifically binds to a consensus AP-1 site and is related to c-fos." Farrel P.J., Rowe D.T., Rooney C.M., Kouzarides T. EMBO J. 8:127-132(1989) [PubMed] [Europe PMC] [Abstract] Cited for: DNA-BINDING.
[4]	"Structure and function of the Epstein-Barr virus BZLF1 protein." Packham G., Economou A., Rooney C.M., Rowe D.T., Farrel P.J. J. Virol. 64:2110-2116(1990) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE, FUNCTION.
[5]	"The BZLF1 protein of EBV has a coiled coil dimerisation domain without a heptad leucine repeat but with homology to the C/EBP leucine zipper." Kouzarides T., Packham G., Cook A., Farrell P.J. Oncogene 6:195-204(1991) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[6]	"A transcription factor with homology to the AP-1 family links RNA transcription and DNA replication in the lytic cycle of Epstein-Barr virus." Schepers A., Pich D., Hammerschmidt W. EMBO J. 12:3921-3929(1993) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[7]	"The PKC targeting protein RACK1 interacts with the Epstein-Barr virus activator protein BZLF1." Baumann M., Gires O., Kolch W., Mischak H., Zeidler R., Pich D., Hammerschmidt W. Eur. J. Biochem. 267:3891-3901(2000) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH HUMAN GNB2L1, SUBCELLULAR LOCATION.
[8]	"Ubinuclein, a novel nuclear protein interacting with cellular and viral transcription factors." Aho S., Buisson M., Pajunen T., Ryoo Y.W., Giot J.-F., Gruffat H., Sergeant A., Uitto J. J. Cell Biol. 148:1165-1176(2000) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH HUMAN UBN1.
[9]	"Epstein-Barr virus BZLF1 gene, a switch from latency to lytic infection, is expressed as an immediate-early gene after primary infection of B lymphocytes." Wen W., Iwakiri D., Yamamoto K., Maruo S., Kanda T., Takada K. J. Virol. 81:1037-1042(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[10]	"TORC2, a coactivator of cAMP-response element-binding protein, promotes Epstein-Barr virus reactivation from latency through interaction with viral BZLF1 protein." Murata T., Sato Y., Nakayama S., Kudoh A., Iwahori S., Isomura H., Tajima M., Hishiki T., Ohshima T., Hijikata M., Shimotohno K., Tsurumi T. J. Biol. Chem. 284:8033-8041(2009) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH HUMAN CRTC2.
[11]	"Interaction of Epstein-Barr virus BZLF1 C-terminal tail structure and core zipper is required for DNA replication but not for promoter transactivation." McDonald C.M., Petosa C., Farrell P.J. J. Virol. 83:3397-3401(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[12]	"Structural basis of lytic cycle activation by the Epstein-Barr virus ZEBRA protein." Petosa C., Morand P., Baudin F., Moulin M., Artero J.B., Muller C.W. Mol. Cell 21:565-572(2006) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.25 ANGSTROMS) OF 175-236.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

V01555 Genomic DNA. Translation: CAA24861.1.
AJ507799 Genomic DNA. Translation: CAD53423.1.
M17547 mRNA. Translation: AAA66529.1.

RefSeq

YP_401673.1. NC_007605.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1ZSD	X-ray	1.70	C	54-64	[»]
2AK4	X-ray	2.50	C/H/M/S	52-64	[»]
2AXF	X-ray	1.80	C	77-86	[»]
2AXG	X-ray	2.00	C	77-86	[»]
2C9L	X-ray	2.25	Y/Z	175-236	[»]
2C9N	X-ray	3.30	Y/Z	175-236	[»]
3KWW	X-ray	2.18	C	52-64	[»]
3KXF	X-ray	3.10	Q/R/S/T	52-64	[»]
3SPV	X-ray	1.30	C	190-197	[»]
3VFN	X-ray	1.50	C	52-64	[»]
3VFO	X-ray	1.70	C	52-64	[»]
3VFP	X-ray	1.85	C	52-64	[»]
3VFR	X-ray	1.85	C	52-64	[»]

ProteinModelPortal

P03206.

SMR

P03206. Positions 178-236.

ModBase

Search...

Protein-protein interaction databases

IntAct

P03206. 3 interactions.

MINT

MINT-6768270.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

3783744.

Phylogenomic databases

ProtClustDB

CLSP2512075.

Family and domain databases

InterPro

IPR004827. bZIP.
IPR017339. Trans-activator_BZLF1_HHV-4.
[Graphical view]

Pfam

PF00170. bZIP_1. 1 hit.
[Graphical view]

PIRSF

PIRSF037966. BZLF1. 1 hit.

SMART

SM00338. BRLZ. 1 hit.
[Graphical view]

PROSITE

PS50217. BZIP. False negative.
PS00036. BZIP_BASIC. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

BindingDB

P03206.

ChEMBL

CHEMBL1293280.

EvolutionaryTrace

P03206.

Entry information

Entry name

BZLF1_EBVB9

Accession

Primary (citable) accession number: P03206
Secondary accession number(s): Q69127, Q777E5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 1, 1989
Last modified:	April 3, 2013
This is version 98 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Family and domain databases

Other

Entry information

Relevant documents