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Protein

Epstein-Barr nuclear antigen 6

Gene

EBNA6

Organism
Epstein-Barr virus (strain B95-8) (HHV-4) (Human herpesvirus 4)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Plays an essential role for the activation and immortalization of human B-cells. Represses transcription of viral promoters TP1 and Cp through interaction with host RBPJ, and inhibits EBNA2-mediated activation of these promoters. Since Cp is the promoter for all EBNA mRNAs, EBNA6 probably contributes to a negative autoregulatory control loop. Alternatively, EBNA6 also regulates the transcription of the EBV oncogene LMP1 in a cell cycle-dependent manner. Modulates the activity of several host proteins involved in cell cycle regulation including host cyclin A, MYC, RB, p21 and p27 mainly through binding to the host SCF(SKP2) complex. Inhibits the promoter of host H2AX and targets H2AX to proteasomal degradation in order to promote latency and cell proliferation.3 Publications

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

G1/S host cell cycle checkpoint dysregulation by virus, Host-virus interaction, Modulation of host cell apoptosis by virus, Modulation of host cell cycle by virus, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Epstein-Barr nuclear antigen 6
Short name:
EBNA-6
Short name:
EBV nuclear antigen 6
Alternative name(s):
Epstein-Barr nuclear antigen 3C
Short name:
EBNA-3C
Short name:
EBV nuclear antigen 3C
Epstein-Barr nuclear antigen 4B
Short name:
EBNA-4B
Short name:
EBV nuclear antigen 4B
Gene namesi
Name:EBNA6
ORF Names:BERF3-BERF4
OrganismiEpstein-Barr virus (strain B95-8) (HHV-4) (Human herpesvirus 4)
Taxonomic identifieri10377 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeGammaherpesvirinaeLymphocryptovirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000007640 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 992992Epstein-Barr nuclear antigen 6PRO_0000116179Add
BLAST

Interactioni

Subunit structurei

Interacts with host CTPB1; this interaction seems important for EBNA6-mediated transcriptional repression. Interacts with host MYC; this interaction enhances MYC stability. Interacts (via N-terminus) with host RBPJ. Interacts (via N-terminus) with host histone H2AX; this interaction facilitates H2AX proteasomal degradation. Interacts with host TP73; this interaction inhibits TP73-mediated apoptotic pathway.5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
MYCP0110611EBI-9255985,EBI-447544From a different organism.
RBPJQ063303EBI-9255985,EBI-632552From a different organism.

Protein-protein interaction databases

BioGridi3509134. 1 interaction.
DIPiDIP-29893N.
IntActiP03204. 6 interactions.
MINTiMINT-97004.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1CG9X-ray2.70C98-106[»]
2BSRX-ray2.30C258-266[»]
3DX6X-ray1.70C281-290[»]
3DX7X-ray1.60C281-290[»]
3DX8X-ray2.10C281-290[»]
3DXAX-ray3.50C/H/M281-290[»]
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP03204.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati551 – 55551-1; approximate
Repeati556 – 56052-1; approximate
Repeati561 – 56553-1; approximate
Repeati566 – 57054-1
Repeati571 – 57555-1
Repeati576 – 58056-1
Repeati581 – 58557-1
Repeati586 – 59058-1
Repeati591 – 59559-1
Repeati596 – 600510-1; approximate
Repeati601 – 605511-1; approximate
Repeati606 – 610512-1
Repeati741 – 753131-2Add
BLAST
Repeati754 – 766132-2Add
BLAST
Repeati767 – 779133-2Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni551 – 6106012 X 5 AA approximate tandem repeats of P-P-A-A-GAdd
BLAST
Regioni741 – 779393 X 13 AA tandem repeats of P-[AP]-P-Q-A-P-Y-Q-G-Y-Q-E-PAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi74 – 807Poly-Arg

Sequence similaritiesi

Belongs to the herpesviridae EBNA-6 family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

KOiK19458.

Family and domain databases

InterProiIPR007706. EBNA-3.
[Graphical view]
PfamiPF05009. EBV-NA3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P03204-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESFEGQGDS RQSPDNERGD NVQTTGEHDQ DPGPGPPSSG ASERLVPEES
60 70 80 90 100
YSRDQQPWGQ SRGDENRGWM QRIRRRRRRR AALSGHLLDT EDNVPPWLPP
110 120 130 140 150
HDITPYTARN IRDAACRAVK QSHLQALSNL ILDSGLDTQH ILCFVMAARQ
160 170 180 190 200
RLQDIRRGPL VAEGGVGWRH WLLTSPSQSW PMGYRTATLR TLTPVPNRVG
210 220 230 240 250
ADSIMLTATF GCQNAARTLN TFSATVWTPP HAGPREQERY AREAEVRFLR
260 270 280 290 300
GKWQRRYRRI YDLIELCGSL HHIWQNLLQT EENLLDFVRF MGVMSSCNNP
310 320 330 340 350
AVNYWFHKTI GNFKPYYPWN APPNENPYHA RRGIKEHVIQ NAFRKAQIQG
360 370 380 390 400
LSMLATGGEP RGDATSETSS DEDTGRQGSD VELESSDDEL PYIDPNMEPV
410 420 430 440 450
QQRPVMFVSR VPAKKPRKLP WPTPKTHPVK RTNVKTSDRS DKAEAQSTPE
460 470 480 490 500
RPGPSEQSSV TVEPAHPTPV EMPMVILHQP PPVPKPVPVK PTPPPSRRRR
510 520 530 540 550
GACVVYDDDV IEVIDVETTE DSSSVSQPNK PHRKHQDGFQ RSGRRQKRAA
560 570 580 590 600
PPTVSPSDTG PPAVGPPAAG PPAAGPPAAG PPAAGPPAAG PPAAGPRILA
610 620 630 640 650
PLSAGPPAAG PHIVTPPSAR PRIMAPPVVR MFMRERQLPQ STGRKPQCFW
660 670 680 690 700
EMRAGREITQ MQQEPSSHLQ SATQPTTPRP SWAPSVCALS VMDAGKAQPI
710 720 730 740 750
ESSHLSSMSP TQPISHEEQP RYEDPDAPLD LSLHPDVAAQ PAPQAPYQGY
760 770 780 790 800
QEPPAPQAPY QGYQEPPPPQ APYQGYQEPP AHGLQSSSYP GYAGPWTPRS
810 820 830 840 850
QHPCYRHPWA PWSQDPVHGH TQGPWDPRAP HLPPQWDGSA GHGQDQVSQF
860 870 880 890 900
PHLQSETGPP RLQLSLVPLV SSSAPSWSSP QPRAPIRPIP TRFPPPPMPL
910 920 930 940 950
QDSMAVGCDS SGTACPSMPF ASDYSQGAFT PLDINATTPK RPRVEESSHG
960 970 980 990
PARCSQATAE AQEILSDNSE ISVFPKDAKQ TDYDASTESE LD
Length:992
Mass (Da):109,129
Last modified:August 1, 1988 - v1
Checksum:i39BEAB9BC515BD84
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01555 Genomic DNA. Translation: CAA24859.1.
AJ507799 Genomic DNA. Translation: CAD53421.1.
RefSeqiYP_401671.1. NC_007605.1.

Genome annotation databases

GeneIDi17494242.
KEGGivg:17494242.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01555 Genomic DNA. Translation: CAA24859.1.
AJ507799 Genomic DNA. Translation: CAD53421.1.
RefSeqiYP_401671.1. NC_007605.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1CG9X-ray2.70C98-106[»]
2BSRX-ray2.30C258-266[»]
3DX6X-ray1.70C281-290[»]
3DX7X-ray1.60C281-290[»]
3DX8X-ray2.10C281-290[»]
3DXAX-ray3.50C/H/M281-290[»]
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi3509134. 1 interaction.
DIPiDIP-29893N.
IntActiP03204. 6 interactions.
MINTiMINT-97004.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi17494242.
KEGGivg:17494242.

Phylogenomic databases

KOiK19458.

Miscellaneous databases

EvolutionaryTraceiP03204.

Family and domain databases

InterProiIPR007706. EBNA-3.
[Graphical view]
PfamiPF05009. EBV-NA3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEBNA6_EBVB9
AccessioniPrimary (citable) accession number: P03204
Secondary accession number(s): Q777E7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: August 1, 1988
Last modified: September 7, 2016
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.