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Protein

Envelope glycoprotein GP350

Gene

BLLF1

Organism
Epstein-Barr virus (strain B95-8) (HHV-4) (Human herpesvirus 4)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Initiates virion attachment to host B-lymphocyte cell, leading to virus entry. Acts by binding to host CR2 at the surface of B-lymphocytes, facilitating the binding of viral glycoprotein gp42 to HLA class II molecules. Attachment triggers virion-host membrane fusion and invasion of the host cell.

GO - Biological processi

Keywordsi

Biological processHost-virus interaction

Names & Taxonomyi

Protein namesi
Recommended name:
Envelope glycoprotein GP350
Alternative name(s):
Membrane antigen
Short name:
MA
Gene namesi
ORF Names:BLLF1
OrganismiEpstein-Barr virus (strain B95-8) (HHV-4) (Human herpesvirus 4)
Taxonomic identifieri10377 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeGammaherpesvirinaeLymphocryptovirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000153037 Componenti: Genome

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 860Virion surfaceSequence analysisAdd BLAST860
Transmembranei861 – 881HelicalSequence analysisAdd BLAST21
Topological domaini882 – 907IntravirionSequence analysisAdd BLAST26

GO - Cellular componenti

Keywords - Cellular componenti

Host membrane, Membrane, Virion

Pathology & Biotechi

Biotechnological usei

Primary surface antigen capable of inducing and reacting with virus-neutralizing antibodies. Almost all EBV candidate vaccines are based on gp350 proteins.

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi21E → A: 40% loss of interaction with host CR2. 1 Publication1
Mutagenesisi22D → A: Complete loss of interaction with host CR2. 1 Publication1
Mutagenesisi151Y → A: Complete loss of interaction with host CR2. 1 Publication1
Mutagenesisi155E → A: 60% loss of interaction with host CR2. 1 Publication1
Mutagenesisi160I → A: Complete loss of interaction with host CR2. 1 Publication1
Mutagenesisi162W → A: Complete loss of interaction with host CR2. 1 Publication1
Mutagenesisi201E → A: 30% loss of interaction with host CR2. 1 Publication1
Mutagenesisi208D → A: 60% loss of interaction with host CR2. 1 Publication1
Mutagenesisi210E → A: 60% loss of interaction with host CR2. 1 Publication1
Mutagenesisi215D → A: 30% loss of interaction with host CR2. 1 Publication1
Mutagenesisi296D → A: Complete loss of interaction with host CR2. 1 Publication1

Chemistry databases

DrugBankiDB00632. Docosanol.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001161831 – 907Envelope glycoprotein GP350Add BLAST907

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi47N-linked (GlcNAc...) asparagine; by hostSequence analysis1
Glycosylationi87N-linked (GlcNAc...) asparagine; by hostSequence analysis1
Glycosylationi114N-linked (GlcNAc...) asparagine; by hostSequence analysis1
Glycosylationi166N-linked (GlcNAc...) asparagine; by hostSequence analysis1
Glycosylationi169N-linked (GlcNAc...) asparagine; by hostSequence analysis1
Glycosylationi195N-linked (GlcNAc...) asparagine; by hostSequence analysis1
Glycosylationi229N-linked (GlcNAc...) asparagine; by hostSequence analysis1
Glycosylationi277N-linked (GlcNAc...) asparagine; by hostSequence analysis1
Glycosylationi318N-linked (GlcNAc...) asparagine; by hostSequence analysis1
Glycosylationi328N-linked (GlcNAc...) asparagine; by hostSequence analysis1
Glycosylationi345N-linked (GlcNAc...) asparagine; by hostSequence analysis1
Glycosylationi356N-linked (GlcNAc...) asparagine; by hostSequence analysis1
Glycosylationi378N-linked (GlcNAc...) asparagine; by hostSequence analysis1
Glycosylationi386N-linked (GlcNAc...) asparagine; by hostSequence analysis1
Glycosylationi411N-linked (GlcNAc...) asparagine; by hostSequence analysis1
Glycosylationi435N-linked (GlcNAc...) asparagine; by hostSequence analysis1
Glycosylationi443N-linked (GlcNAc...) asparagine; by hostSequence analysis1
Glycosylationi457N-linked (GlcNAc...) asparagine; by hostSequence analysis1
Glycosylationi497N-linked (GlcNAc...) asparagine; by hostSequence analysis1
Glycosylationi519N-linked (GlcNAc...) asparagine; by hostSequence analysis1
Glycosylationi533N-linked (GlcNAc...) asparagine; by hostSequence analysis1
Glycosylationi547N-linked (GlcNAc...) asparagine; by hostSequence analysis1
Glycosylationi568N-linked (GlcNAc...) asparagine; by hostSequence analysis1
Glycosylationi589N-linked (GlcNAc...) asparagine; by hostSequence analysis1
Glycosylationi610N-linked (GlcNAc...) asparagine; by hostSequence analysis1
Glycosylationi624N-linked (GlcNAc...) asparagine; by hostSequence analysis1
Glycosylationi627N-linked (GlcNAc...) asparagine; by hostSequence analysis1
Glycosylationi645N-linked (GlcNAc...) asparagine; by hostSequence analysis1
Glycosylationi656N-linked (GlcNAc...) asparagine; by hostSequence analysis1
Glycosylationi683N-linked (GlcNAc...) asparagine; by hostSequence analysis1
Glycosylationi701N-linked (GlcNAc...) asparagine; by hostSequence analysis1
Glycosylationi735N-linked (GlcNAc...) asparagine; by hostSequence analysis1
Glycosylationi746N-linked (GlcNAc...) asparagine; by hostSequence analysis1
Glycosylationi755N-linked (GlcNAc...) asparagine; by hostSequence analysis1
Glycosylationi780N-linked (GlcNAc...) asparagine; by hostSequence analysis1
Glycosylationi815N-linked (GlcNAc...) asparagine; by hostSequence analysis1
Glycosylationi858N-linked (GlcNAc...) asparagine; by hostSequence analysis1

Post-translational modificationi

Extensively glycosylated.1 Publication

Keywords - PTMi

Glycoprotein

Expressioni

Keywords - Developmental stagei

Late protein

Interactioni

Subunit structurei

Interacts with host CR2.2 Publications

Protein-protein interaction databases

IntActiP03200. 2 interactors.
MINTiMINT-6768180.

Structurei

3D structure databases

ProteinModelPortaliP03200.
SMRiP03200.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Epstein-Barr GP350 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

KOiK19450.
OrthoDBiVOG0900003J.

Family and domain databases

InterProiView protein in InterPro
IPR007796. Herpes_BLLF1.
PfamiView protein in Pfam
PF05109. Herpes_BLLF1. 2 hits.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform GP350 (identifier: P03200-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEAALLVCQY TIQSLIHLTG EDPGFFNVEI PEFPFYPTCN VCTADVNVTI
60 70 80 90 100
NFDVGGKKHQ LDLDFGQLTP HTKAVYQPRG AFGGSENATN LFLLELLGAG
110 120 130 140 150
ELALTMRSKK LPINVTTGEE QQVSLESVDV YFQDVFGTMW CHHAEMQNPV
160 170 180 190 200
YLIPETVPYI KWDNCNSTNI TAVVRAQGLD VTLPLSLPTS AQDSNFSVKT
210 220 230 240 250
EMLGNEIDIE CIMEDGEISQ VLPGDNKFNI TCSGYESHVP SGGILTSTSP
260 270 280 290 300
VATPIPGTGY AYSLRLTPRP VSRFLGNNSI LYVFYSGNGP KASGGDYCIQ
310 320 330 340 350
SNIVFSDEIP ASQDMPTNTT DITYVGDNAT YSVPMVTSED ANSPNVTVTA
360 370 380 390 400
FWAWPNNTET DFKCKWTLTS GTPSGCENIS GAFASNRTFD ITVSGLGTAP
410 420 430 440 450
KTLIITRTAT NATTTTHKVI FSKAPESTTT SPTLNTTGFA DPNTTTGLPS
460 470 480 490 500
STHVPTNLTA PASTGPTVST ADVTSPTPAG TTSGASPVTP SPSPWDNGTE
510 520 530 540 550
SKAPDMTSST SPVTTPTPNA TSPTPAVTTP TPNATSPTPA VTTPTPNATS
560 570 580 590 600
PTLGKTSPTS AVTTPTPNAT SPTLGKTSPT SAVTTPTPNA TSPTLGKTSP
610 620 630 640 650
TSAVTTPTPN ATGPTVGETS PQANATNHTL GGTSPTPVVT SQPKNATSAV
660 670 680 690 700
TTGQHNITSS STSSMSLRPS SNPETLSPST SDNSTSHMPL LTSAHPTGGE
710 720 730 740 750
NITQVTPASI STHHVSTSSP APRPGTTSQA SGPGNSSTST KPGEVNVTKG
760 770 780 790 800
TPPQNATSPQ APSGQKTAVP TVTSTGGKAN STTGGKHTTG HGARTSTEPT
810 820 830 840 850
TDYGGDSTTP RPRYNATTYL PPSTSSKLRP RWTFTSPPVT TAQATVPVPP
860 870 880 890 900
TSQPRFSNLS MLVLQWASLA VLTLLLLLVM ADCAFRRNLS TSHTYTTPPY

DDAETYV
Length:907
Mass (Da):94,432
Last modified:July 21, 1986 - v1
Checksum:i0750141CBCAC52C9
GO
Isoform GP220 (identifier: P03200-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     502-750: Missing.

Show »
Length:658
Mass (Da):70,085
Checksum:i307AA6B5CF54132F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti721A → E in AAA45881 (PubMed:2987520).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_002070502 – 750Missing in isoform GP220. CuratedAdd BLAST249

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01555 Genomic DNA. Translation: CAA24854.1.
AJ507799 Genomic DNA. Translation: CAD53417.1.
M10593 Genomic DNA. Translation: AAA45881.1.
M10593 Genomic DNA. Translation: AAA45880.1.
PIRiA43042. QQBE21.
B43042. QQBE22.
RefSeqiYP_401667.1. NC_007605.1.

Genome annotation databases

GeneIDi3783713.
KEGGivg:3783713.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiGP350_EBVB9
AccessioniPrimary (citable) accession number: P03200
Secondary accession number(s): P03201
, Q66536, Q66537, Q777F0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: May 10, 2017
This is version 89 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families