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P03186

- DEN_EBVB9

UniProt

P03186 - DEN_EBVB9

Protein

Deneddylase

Gene

BPLF1

Organism
Epstein-Barr virus (strain B95-8) (HHV-4) (Human herpesvirus 4)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 72 (01 Oct 2014)
      Sequence version 1 (21 Jul 1986)
      Previous versions | rss
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    Functioni

    Deneddylase that deregulates the host cell cycle S phase to create a favorable environment allowing efficient viral genome replication. Interacts with and deneddylates host cullins including CUL1 and CUL4A, thereby reducing their E3 ubiquitin ligase activity. Inhibition of cullins leads to the stabilization of cullin-RING ligase substrates such as host CDN1A/p21, CDKN1B/p27kip and CDC25A, preventing S phase progression. Additionally, acts as a deubiquitinase and cleaves both 'Lys-48' and 'Lys-63'-linked ubiquitin chains.2 Publications

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei48 – 481Important for catalytic activityPROSITE-ProRule annotation
    Active sitei61 – 611PROSITE-ProRule annotation
    Active sitei193 – 1931PROSITE-ProRule annotation
    Active sitei195 – 1951PROSITE-ProRule annotation

    GO - Molecular functioni

    1. cysteine-type peptidase activity Source: UniProtKB-KW

    GO - Biological processi

    1. modulation by virus of host protein ubiquitination Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Host-virus interaction, Modulation of host ubiquitin pathway by viral deubiquitinase, Modulation of host ubiquitin pathway by virus, Ubl conjugation pathway

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Deneddylase (EC:3.4.19.12, EC:3.4.22.-)
    Alternative name(s):
    Large tegument protein
    Short name:
    LTP
    Gene namesi
    ORF Names:BPLF1
    OrganismiEpstein-Barr virus (strain B95-8) (HHV-4) (Human herpesvirus 4)
    Taxonomic identifieri10377 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeGammaherpesvirinaeLymphocryptovirus
    Virus hostiHomo sapiens (Human) [TaxID: 9606]
    ProteomesiUP000007640: Genome

    Subcellular locationi

    Virion tegument 1 Publication. Host cytoplasm 1 Publication. Host nucleus By similarity

    GO - Cellular componenti

    1. host cell cytoplasm Source: UniProtKB-SubCell
    2. host cell nucleus Source: UniProtKB-SubCell
    3. viral tegument Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host cytoplasm, Host nucleus, Virion, Virion tegument

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi61 – 611C → A: Complete loss of ubiquitin and NEDD8 binding. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 31493149DeneddylasePRO_0000116038Add
    BLAST

    Interactioni

    Subunit structurei

    Interacts with host CUL1 and CUL4A; these interactions inhibit the E3 ligase activity of cullins.

    Protein-protein interaction databases

    BioGridi971768. 10 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliP03186.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini41 – 258218Peptidase C76PROSITE-ProRule annotationAdd
    BLAST
    Repeati335 – 33951
    Repeati340 – 34452
    Repeati345 – 34953
    Repeati350 – 35454
    Repeati355 – 35955
    Repeati360 – 36456
    Repeati365 – 36957
    Repeati370 – 37458

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 269269Deubiquitination activityBy similarityAdd
    BLAST
    Regioni335 – 374408 X 5 AA repeats of P-A-S-A-AAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili1100 – 113031Sequence AnalysisAdd
    BLAST
    Coiled coili1394 – 144653Sequence AnalysisAdd
    BLAST
    Coiled coili1706 – 173530Sequence AnalysisAdd
    BLAST
    Coiled coili1875 – 190430Sequence AnalysisAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi412 – 4187Nuclear localization signalBy similarity

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi336 – 37641Ala-richAdd
    BLAST
    Compositional biasi527 – 5304Poly-Ala
    Compositional biasi532 – 5354Poly-Thr
    Compositional biasi1356 – 13594Poly-Asn
    Compositional biasi1475 – 14839Poly-Ala
    Compositional biasi1558 – 15636Poly-Gly
    Compositional biasi1656 – 16594Poly-Pro
    Compositional biasi1830 – 18334Poly-Ala
    Compositional biasi2085 – 20884Poly-Arg
    Compositional biasi2100 – 21034Poly-Ala
    Compositional biasi2845 – 2948104Ala-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the herpesviridae deneddylase family.Curated
    Contains 1 peptidase C76 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil, Repeat

    Family and domain databases

    InterProiIPR006928. Pept_C76_UL36-USP.
    [Graphical view]
    PfamiPF04843. Herpes_teg_N. 1 hit.
    [Graphical view]
    PROSITEiPS51521. HTUSP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P03186-1 [UniParc]FASTAAdd to Basket

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    MSNGDWGQSQ RTRGTGPVRG IRTMDVNAPG GGSGGSALRI LGTASCNQAH     50
    CKFGRFAGIQ CVSNCVLYLV KSFLAGRPLT SRPELDEVLD EGARLDALMR 100
    QSGILKGHEM AQLTDVPSSV VLRGGGRVHI YRSAEIFGLV LFPAQIANSA 150
    VVQSLAEVLH GSYNGVAQFI LYICDIYAGA IIIETDGSFY LFDPHCQKDA 200
    APGTPAHVRV STYAHDILQY VGAPGAQYTC VHLYFLPEAF ETEDPRIFML 250
    EHYGVYDFYE ANGSGFDLVG PELVSSDGEA AGTPGADSSP PVMLPFERRI 300
    IPYNLRPLPS RSFTSDSFPA ARYSPAKTNS PPSSPASAAP ASAAPASAAP 350
    ASAAPASAAP ASAAPASAAP ASAAPASSPP LFIPIPGLGH TPGVPAPSTP 400
    PRASSGAAPQ TPKRKKGLGK DSPHKKPTSG RRLPLSSTTD TEDDQLPRTH 450
    VPPHRPPSAA RLPPPVIPIP HQSPPASPTP HPAPVSTIAP SVTPSPRLPL 500
    QIPIPLPQAA PSNPKIPLTT PSPSPTAAAA PTTTTLSPPP TQQQPPQSAA 550
    PAPSPLLPQQ QPTPSAAPAP SPLLPQQQPP PSAARAPSPL PPQQQPLPSA 600
    TPAPPPAQQL PPSATTLEPE KNHPPAADRA GTEISPSPPF GQQPSFGDDA 650
    SGGSGLVRYL SDLEEPFLSM SDSEEAESDL ASDIPTTEDE DMFEDEVFSN 700
    SLESGSSAPT SPITLDTARS QYYQTTFDIE TPEMDFVPLE SNIARIAGHT 750
    YQEQAIVYDP ASNREVPEAD ALSMIDYLLV TVVLEQGLIR SRDRSSVLNL 800
    LEFLKDWSGH LQVPTLDLEQ LLTSELNIQN LANMLSENKG RAGEFHKHLA 850
    AKLEACLPSL ATKDAVRVDA GAKMLAEIPQ LAESDDGKFD LEAARRRLTD 900
    LLSGGDQEAG EGGGEPEDNS IYRGPHVDVP LVLDDESWKR LLSLAEAART 950
    AVARQQAGVD EEDVRFLALL TAIEYGAPPA ASVPPFVHNV AVRSKNAALH 1000
    VRRCTADIRD KVASAASDYL SYLEDPSLPT VMDFDDLLTH LRHTCQIIAS 1050
    LPLLNIRYTS IEWDYRELLY LGTALSDMSG IPWPLERVEE DDPSIAPLPE 1100
    FETVAKKQKE LETTRENEKR LRTILDDIEA MLGLAGVASA PGAPISPASP 1150
    SATPANHDNP EATPPLADTA ALTIPVIEKY IANAGSIVGA AKNPTYIRLR 1200
    DTIQQIVRSK KYLMNILKSI TFYTIDNYIA SFEESIDHLY RDLPVLDPEV 1250
    QDGIDRILDP MVSEALHTFE MGNRLTLEPA RLVALQNFAT HSTLKETAAA 1300
    VNLLPGLLAV YDATITGQAP EDALRLLSGL QNQLSQTLIP GKLKKRFLSY 1350
    LQKLKNNNND QLRQKEVQAW RLEAEGFKPA TEEQLEAFLD TAPNKELKRQ 1400
    YEKKLRQLME TGRKEKEKLR EQEDKERQER RAREANEAWA RIRKALGARP 1450
    EPAPTSPDDW NTLLASLLPD NTDSAAAAAA AVARNTDILD SLTQILAAML 1500
    LGITRVRRER LRSLLVDDGG AAERMEAAEP GWFTDIETGP LARLDAWPAT 1550
    PAATAKEGGG GRGAEEAAGA LFRARTAADA IRSALAQTRQ ALQSPDMKSA 1600
    VVNTDLEAPY AEYERGLAGL LEKRRAAEAA LTAIVSEYVD RTLPEATNDP 1650
    GQANLPPPPT IPQATAPPRL ASDSALWPKK PQLLTRRERD DLLQATGDFF 1700
    SELLTEAEAA EVRALEEQVR ESQTLMAKAH EMAASTRRGF HTALEAVLSR 1750
    SRDEAPDDEL RSLLPSPPKA PVQAPLEAAL ARAAAGNGSW PYRKSLAAAK 1800
    WIRGICEAVR GLSEGALALA GGAGAWLNLA AAADGEIHEL TRLLEVEGMA 1850
    QNSMDGMEEL RLALATLDPK RVAGGKETVA DWKRRLSRLE AIIQEAQEES 1900
    QLQGTLQDLV TQARGHTDPR QLKIVVEAAR GLALGASAGS QYALLKDKLL 1950
    RYASAKQSFL AFYETAQPTV FVKHPLTNNL PLLITISAPP TGWGNGAPTR 2000
    RAQFLAAAGP AKYAGTLWLE TESPCDPLNP AYVSADTQEP LNYIPVYHNF 2050
    LEYVMPTVLE NPEAFSLTPA GRPQAIGPPQ DDQERRRRTL ASVASARLSA 2100
    AAADSYWDTW PDVESNAGEL LREYVSAPKA LMEDLADNPI VAMTLLAHAS 2150
    LIASRNHPPY PAPATDREVI LLEQREMMAL LVGTHPAYAA AFLGAPSFYA 2200
    GLGLVSALAR DGGLGDLLSD SVLTYRLVRS PASGRGGMPS TTRGSNDGED 2250
    ARRLTRHRIA GPPTGFIFFQ DAWEEMDTRA ALWPHPEFLG LVHNQSTARA 2300
    RACMLLLARR CFAPEALQQL WHSLRPLEGP VAFQDYLRDF VKQAYTRGEE 2350
    LPRAEGLEVP RETPSSYGTV TGRALRNLMP YGTPITGPKR GSGDTIPVSV 2400
    FEAAVAAAFL GRPLTLFVSS QYLFNLKTLG QVRVVAPLLY CDGHSEPFRS 2450
    LVETISLNFL QDLDGYSESF EPEMSIFARQ AVWLRELLTE ARAAKPKEAR 2500
    PPTVAILANR KNIIWKCFTY RHNLPDVQFY FNAAGASRWP TDVLNPSFYE 2550
    HEDPPLPVGY QLPPNPRNVQ ELFSGFPPRV GHGLVSGDGF QSADNTPASS 2600
    DRLQQLGGGE TDQGEKGSTT AESEASGPPS PQSPLLEKVA PGRPRDWLSP 2650
    TSSPRDVTVT PGLAAPITLP GPRLMARPYF GAETRASESP DRSPGSSPRP 2700
    WPKDSLELLP QPAPQQPPSS PWASEQGPIV YTLSPHSTPS TASGSQKKHT 2750
    IQIPGLVPSQ KPSYPPSAPY KPGQSTGGIA PTPSAASLTT FGLQPQDTQA 2800
    SSQDPPYGHS IMQREKKQQG GREEAAEIRP SATRLPTAVG LRPRAPVVAA 2850
    GAAASATPAF DPGEAPSGFP IPQAPALGSG LAAPAHTPVG ALAPRPQKTQ 2900
    AQRPQDAAAL PTPTIKAVGA RPVPKATGAL AAGARPRGQP TAAPPSAASP 2950
    PRVSLPVRSR QQQSPAIPLP PMHSGSEPGA RPEVRLSQYR HAGPQTYTVR 3000
    KEAPPSAASQ LPKMPKCKDS MYYPPSGSAR YPAPFQALSF SQSVASPAPS 3050
    SDQTTLLWNT PSVVTQFLSI EDIIREVVTG GSTSGDLVVP SGSPSSLSTA 3100
    APEQDLRYSL TLSQASRVLS RFVSQLRRKL ERSTHRLIAD LERLKFLYL 3149
    Length:3,149
    Mass (Da):337,959
    Last modified:July 21, 1986 - v1
    Checksum:i3DD0C576587313D8
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    V01555 Genomic DNA. Translation: CAA24839.1.
    AJ507799 Genomic DNA. Translation: CAD53402.1.
    PIRiG93065. QQBE8.
    RefSeqiYP_401652.1. NC_007605.1.

    Genome annotation databases

    GeneIDi3783726.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    V01555 Genomic DNA. Translation: CAA24839.1 .
    AJ507799 Genomic DNA. Translation: CAD53402.1 .
    PIRi G93065. QQBE8.
    RefSeqi YP_401652.1. NC_007605.1.

    3D structure databases

    ProteinModelPortali P03186.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 971768. 10 interactions.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 3783726.

    Family and domain databases

    InterProi IPR006928. Pept_C76_UL36-USP.
    [Graphical view ]
    Pfami PF04843. Herpes_teg_N. 1 hit.
    [Graphical view ]
    PROSITEi PS51521. HTUSP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    2. Cited for: SUBCELLULAR LOCATION.
    3. "The EBV deubiquitinating enzyme, BPLF1, reduces EBV ribonucleotide reductase activity."
      Whitehurst C.B., Ning S., Bentz G.L., Dufour F., Gershburg E., Shackelford J., Langelier Y., Pagano J.S.
      J. Virol. 83:4345-4353(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    4. "A deneddylase encoded by Epstein-Barr virus promotes viral DNA replication by regulating the activity of cullin-RING ligases."
      Gastaldello S., Hildebrand S., Faridani O., Callegari S., Palmkvist M., Di Guglielmo C., Masucci M.G.
      Nat. Cell Biol. 12:351-361(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, MUTAGENESIS OF CYS-61.

    Entry informationi

    Entry nameiDEN_EBVB9
    AccessioniPrimary (citable) accession number: P03186
    Secondary accession number(s): Q777G4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: July 21, 1986
    Last modified: October 1, 2014
    This is version 72 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3