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Protein

Nuclear egress protein 2

Gene

NEC2

Organism
Epstein-Barr virus (strain B95-8) (HHV-4) (Human herpesvirus 4)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Plays an essential role in virion nuclear egress, the first step of virion release from infected cell. Within the host nucleus, NEC1 interacts with the newly formed capsid through the vertexes and directs it to the inner nuclear membrane by associating with NEC2. Induces the budding of the capsid at the inner nuclear membrane as well as its envelopment into the perinuclear space. There, the NEC1/NEC2 complex promotes the fusion of the enveloped capsid with the outer nuclear membrane and the subsequent release of the viral capsid into the cytoplasm where it will reach the secondary budding sites in the host Golgi or trans-Golgi network.UniRule annotation2 Publications

GO - Biological processi

  • viral budding from nuclear membrane Source: UniProtKB
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear egress protein 2UniRule annotation
Gene namesi
Name:NEC2UniRule annotation
ORF Names:BFRF1
OrganismiEpstein-Barr virus (strain B95-8) (HHV-4) (Human herpesvirus 4)
Taxonomic identifieri10377 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeGammaherpesvirinaeLymphocryptovirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000007640 Componenti: Genome

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 315Perinuclear spaceUniRule annotationAdd BLAST315
Transmembranei316 – 333HelicalUniRule annotationAdd BLAST18
Topological domaini334 – 336NuclearUniRule annotation3

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host membrane, Host nucleus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001160331 – 336Nuclear egress protein 2Add BLAST336

Post-translational modificationi

Phosphorylated.UniRule annotation

Keywords - PTMi

Phosphoprotein

Expressioni

Keywords - Developmental stagei

Late protein

Interactioni

Subunit structurei

Forms a heterohexameric complex with NEC1.UniRule annotation2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
NEC1P0CK473EBI-2620196,EBI-2620189

Protein-protein interaction databases

IntActiP03185. 17 interactors.

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi206 – 209Poly-Gly4

Sequence similaritiesi

Belongs to the herpesviridae NEC2 protein family.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Family and domain databases

HAMAPiMF_04024. HSV_NEC2. 1 hit.
InterProiIPR007626. Herpesvirus_viron_egress-type.
[Graphical view]
PfamiPF04541. Herpes_U34. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P03185-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASPEERLLD ELNNVIVSFL CDSGSLEVER CSGAHVFSRG SSQPLCTVKL
60 70 80 90 100
RHGQIYHLEF VYKFLAFKLK NCNYPSSPVF VISNNGLATT LRCFLHEPSG
110 120 130 140 150
LRSGQSGPCL GLSTDVDLPK NSIIMLGQDD FIKFKSPLVF PAELDLLKSM
160 170 180 190 200
VVCRAYITEH RTTMQFLVFQ AANAQKASRV MDMISDMSQQ LSRSGQVEDT
210 220 230 240 250
GARVTGGGGP RPGVTHSGCL GDSHVRGRGG WDLDNFSEAE TEDEASYAPW
260 270 280 290 300
RDKDSWSESE AAPWKKELVR HPIRRHRTRE TRRMRGSHSR VEHVPPETRE
310 320 330
TVVGGAWRYS WRATPYLARV LAVTAVALLL MFLRWT
Length:336
Mass (Da):37,632
Last modified:July 21, 1986 - v1
Checksum:i0F2E3430DA95A944
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01555 Genomic DNA. Translation: CAA24879.1.
M11923 Genomic DNA. Translation: AAA45869.1.
AJ507799 Genomic DNA. Translation: CAD53399.1.
PIRiF93065. QQBE7.
RefSeqiYP_401649.1. NC_007605.1.

Genome annotation databases

GeneIDi3783699.
KEGGivg:3783699.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01555 Genomic DNA. Translation: CAA24879.1.
M11923 Genomic DNA. Translation: AAA45869.1.
AJ507799 Genomic DNA. Translation: CAD53399.1.
PIRiF93065. QQBE7.
RefSeqiYP_401649.1. NC_007605.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP03185. 17 interactors.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi3783699.
KEGGivg:3783699.

Family and domain databases

HAMAPiMF_04024. HSV_NEC2. 1 hit.
InterProiIPR007626. Herpesvirus_viron_egress-type.
[Graphical view]
PfamiPF04541. Herpes_U34. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNEC2_EBVB9
AccessioniPrimary (citable) accession number: P03185
Secondary accession number(s): Q777G7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: November 30, 2016
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.