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Protein

Apoptosis regulator BHRF1

Gene

BHRF1

Organism
Epstein-Barr virus (strain B95-8) (HHV-4) (Human herpesvirus 4)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Prevents premature death of the host cell during virus production, which would otherwise reduce the amount of progeny virus. Acts as a host B-cell leukemia/lymphoma 2 (Bcl-2) homolog, and interacts with pro-apoptotic proteins to prevent mitochondria permeabilization, release of cytochrome c and subsequent apoptosis of the host cell.

GO - Biological processi

  • negative regulation by symbiont of host apoptotic process Source: CACAO
  • negative regulation of release of cytochrome c from mitochondria Source: CACAO
Complete GO annotation...

Keywords - Biological processi

Apoptosis

Names & Taxonomyi

Protein namesi
Recommended name:
Apoptosis regulator BHRF1
Alternative name(s):
Early antigen protein R
Short name:
EA-R
Nuclear antigen
Gene namesi
ORF Names:BHRF1
OrganismiEpstein-Barr virus (strain B95-8) (HHV-4) (Human herpesvirus 4)
Taxonomic identifieri10377 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeGammaherpesvirinaeLymphocryptovirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000007640 Componenti: Genome

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei166 – 18621HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host membrane, Host mitochondrion, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 191191Apoptosis regulator BHRF1PRO_0000143091Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi22 – 221N-linked (GlcNAc...); by hostSequence analysis
Glycosylationi118 – 1181N-linked (GlcNAc...); by hostSequence analysis

Keywords - PTMi

Glycoprotein

Expressioni

Keywords - Developmental stagei

Early protein

Interactioni

Subunit structurei

Interacts with isoform 1 of host VRK2; this interaction is involved in protecting cells from apoptosis. Interacts with host PRA1; this interaction seems to modulate BHRF1 anti-apoptotic activity. Interacts with host BCL2L11.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
VRK2Q86Y077EBI-1207659,EBI-1207615From a different organism.

Protein-protein interaction databases

DIPiDIP-39168N.
IntActiP03182. 1 interaction.
MINTiMINT-199358.

Structurei

Secondary structure

1
191
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi5 – 2016Combined sources
Beta strandi22 – 243Combined sources
Helixi27 – 359Combined sources
Helixi45 – 6016Combined sources
Helixi62 – 7514Combined sources
Helixi79 – 9113Combined sources
Helixi98 – 11720Combined sources
Beta strandi118 – 1214Combined sources
Helixi123 – 13715Combined sources
Helixi138 – 1403Combined sources
Helixi141 – 1466Combined sources
Helixi150 – 1556Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1Q59NMR-A1-160[»]
2XPXX-ray2.05A1-160[»]
ProteinModelPortaliP03182.
SMRiP03182. Positions 1-160.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP03182.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 1818Interaction with host VRK2Add
BLAST
Regioni89 – 14254Interaction with host VRK2Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi89 – 10921BH1Add
BLAST
Motifi142 – 15716BH2Add
BLAST

Sequence similaritiesi

Belongs to the Bcl-2 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

KOiK19463.

Family and domain databases

InterProiIPR002475. Bcl2-like.
IPR020726. Bcl2_BH2_motif_CS.
IPR026298. Blc2_fam.
[Graphical view]
PfamiPF00452. Bcl-2. 1 hit.
[Graphical view]
PROSITEiPS50062. BCL2_FAMILY. 1 hit.
PS01258. BH2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P03182-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAYSTREILL ALCIRDSRVH GNGTLHPVLE LAARETPLRL SPEDTVVLRY
60 70 80 90 100
HVLLEEIIER NSETFTETWN RFITHTEHVD LDFNSVFLEI FHRGDPSLGR
110 120 130 140 150
ALAWMAWCMH ACRTLCCNQS TPYYVVDLSV RGMLEASEGL DGWIHQQGGW
160 170 180 190
STLIEDNIPG SRRFSWTLFL AGLTLSLLVI CSYLFISRGR H
Length:191
Mass (Da):21,893
Last modified:July 21, 1986 - v1
Checksum:i8108BCB94F81DC8B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M17416 Genomic DNA. Translation: AAA45873.1.
V01555 Genomic DNA. No translation available.
M17293 Genomic DNA. Translation: AAA45875.1.
AJ507799 Genomic DNA. Translation: CAD53396.1.
EF192979 Genomic DNA. Translation: ABM92330.1.
PIRiC93065. QQBE4.
RefSeqiYP_401646.1. NC_007605.1.

Genome annotation databases

GeneIDi3783706.
KEGGivg:3783706.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M17416 Genomic DNA. Translation: AAA45873.1.
V01555 Genomic DNA. No translation available.
M17293 Genomic DNA. Translation: AAA45875.1.
AJ507799 Genomic DNA. Translation: CAD53396.1.
EF192979 Genomic DNA. Translation: ABM92330.1.
PIRiC93065. QQBE4.
RefSeqiYP_401646.1. NC_007605.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1Q59NMR-A1-160[»]
2XPXX-ray2.05A1-160[»]
ProteinModelPortaliP03182.
SMRiP03182. Positions 1-160.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-39168N.
IntActiP03182. 1 interaction.
MINTiMINT-199358.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi3783706.
KEGGivg:3783706.

Phylogenomic databases

KOiK19463.

Miscellaneous databases

EvolutionaryTraceiP03182.

Family and domain databases

InterProiIPR002475. Bcl2-like.
IPR020726. Bcl2_BH2_motif_CS.
IPR026298. Blc2_fam.
[Graphical view]
PfamiPF00452. Bcl-2. 1 hit.
[Graphical view]
PROSITEiPS50062. BCL2_FAMILY. 1 hit.
PS01258. BH2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. "Isolation and characterization of cDNA clones corresponding to transcripts from the BamHI H and F regions of the Epstein-Barr virus genome."
    Pfitzner A.J., Tsai E.C., Strominger J.L., Speck S.H.
    J. Virol. 61:2902-2909(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Epstein-Barr virus BHRF1 micro- and stable RNAs during latency III and after induction of replication."
    Xing L., Kieff E.
    J. Virol. 81:9967-9975(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Akata.
  4. "Identification of an Epstein-Barr virus early gene encoding a second component of the restricted early antigen complex."
    Pearson G.R., Luka J., Petti L., Sample J., Birkenbach M., Braun D., Kieff E.
    Virology 160:151-161(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  5. "BHRF1, a viral homologue of the Bcl-2 oncogene, is conserved at both the sequence and functional level in different Epstein-Barr virus isolates."
    Khanim F., Dawson C., Meseda C.A., Dawson J., Mackett M., Young L.S.
    J. Gen. Virol. 78:2987-2999(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  6. "The cellular protein PRA1 modulates the anti-apoptotic activity of Epstein-Barr virus BHRF1, a homologue of Bcl-2, through direct interaction."
    Li L.Y., Shih H.M., Liu M.Y., Chen J.Y.
    J. Biol. Chem. 276:27354-27362(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH HUMAN PRA1.
  7. "Human cellular protein VRK2 interacts specifically with Epstein-Barr virus BHRF1, a homologue of Bcl-2, and enhances cell survival."
    Li L.Y., Liu M.Y., Shih H.M., Tsai C.H., Chen J.Y.
    J. Gen. Virol. 87:2869-2878(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH HUMAN VRK2.
  8. "BH3 domains define selective inhibitory interactions with BHRF-1 and KSHV BCL-2."
    Flanagan A.M., Letai A.
    Cell Death Differ. 15:580-588(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH HUMAN BCL2L11.
  9. "Solution structure of the BHRF1 protein from Epstein-Barr virus, a homolog of human Bcl-2."
    Huang Q., Petros A.M., Virgin H.W., Fesik S.W., Olejniczak E.T.
    J. Mol. Biol. 332:1123-1130(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 1-160.

Entry informationi

Entry nameiEAR_EBVB9
AccessioniPrimary (citable) accession number: P03182
Secondary accession number(s): Q777H0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: June 8, 2016
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

EA-R is part of the restricted EA-complex.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.