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Protein

Thymidine kinase

Gene

TK

Organism
Epstein-Barr virus (strain B95-8) (HHV-4) (Human herpesvirus 4)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

In latent infection, may allow the virus to be reactivated and to grow in cells lacking a high concentration of phosphorylated nucleic acid precursors, such as memory B-cells that do not replicate their genome. Catalyzes the transfer of the gamma-phospho group of ATP to thymidine to generate dTMP in the salvage pathway of pyrimidine synthesis. The dTMP serves as a substrate for DNA polymerase during DNA replication (By similarity).By similarity

Catalytic activityi

ATP + thymidine = ADP + thymidine 5'-phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei317 – 3171Proton acceptorSequence Analysis
Binding sitei355 – 3551SubstrateBy similarity
Binding sitei402 – 4021SubstrateBy similarity
Binding sitei445 – 4451ATPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi291 – 2988ATPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

DNA synthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

SABIO-RKP03177.

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidine kinase (EC:2.7.1.21)
Gene namesi
Name:TK
ORF Names:BXLF1
OrganismiEpstein-Barr virus (strain B95-8) (HHV-4) (Human herpesvirus 4)
Taxonomic identifieri10377 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeGammaherpesvirinaeLymphocryptovirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
ProteomesiUP000007640 Componenti: Genome

Subcellular locationi

  • Virion tegument
  • Host nucleus

  • Note: Localizes to the centrosome and more precisely to the periphery of the centriole, tightly encircling the tubulin-rich centrioles.

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host nucleus, Virion, Virion tegument

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 607607Thymidine kinasePRO_0000175059Add
BLAST

Expressioni

Keywords - Developmental stagei

Early protein

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP03177.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR001889. Herpes_TK.
IPR013672. Herpes_TK_C.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00693. Herpes_TK. 1 hit.
PF08465. Herpes_TK_C. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

P03177-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGFPGKEAG PPGGWRKCQE DESPENERHE NFYAEIDDFA PSVLTPTGSD
60 70 80 90 100
SGAGEEDDDG LYQVPTHWPP LMAPTGLSGE RVPCRTQAAV TSNTGNSPGS
110 120 130 140 150
RHTSCPFTLP RGAQPPAPAH QKPTAPTPKP RSRECGPSKT PDPFSWFRKT
160 170 180 190 200
SCTEGGADST SRSFMYQKGF EEGLAGLGLD DKSDCESEDE SNFRRPSSHS
210 220 230 240 250
ALKQKNGGKG KPSGLFEHLA AHGREFSKLS KHAAQLKRLS GSVMNVLNLD
260 270 280 290 300
DAQDTRQAKA QRKESMRVPI VTHLTNHVPV IKPACSLFLE GAPGVGKTTM
310 320 330 340 350
LNHLKAVFGD LTIVVPEPMR YWTHVYENAI KAMHKNVTRA RHGREDTSAE
360 370 380 390 400
VLACQMKFTT PFRVLASRKR SLLVTESGAR SVAPLDCWIL HDRHLLSASV
410 420 430 440 450
VFPLMLLRSQ LLSYSDFIQV LATFTADPGD TIVWMKLNVE ENMRRLKKRG
460 470 480 490 500
RKHESGLDAG YLKSVNDAYH AVYCAWLLTQ YFAPEDIVKV CAGLTTITTV
510 520 530 540 550
CHQSHTPIIR SGVAEKLYKN SIFSVLKEVI QPFRADAVLL EVCLAFTRTL
560 570 580 590 600
AYLQFVLVDL SEFQDDLPGC WTEIYMQALK NPAIRSQFFD WAGLSKVISD

FERGNRD
Length:607
Mass (Da):67,193
Last modified:November 1, 1995 - v2
Checksum:i97A4CCDB598A09F1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01555 Genomic DNA. Translation: CAA24799.1.
AJ507799 Genomic DNA. Translation: CAD53451.1.
PIRiA00615. KIBETE.
RefSeqiYP_401701.1. NC_007605.1.

Genome annotation databases

GeneIDi3783741.
KEGGivg:3783741.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01555 Genomic DNA. Translation: CAA24799.1.
AJ507799 Genomic DNA. Translation: CAD53451.1.
PIRiA00615. KIBETE.
RefSeqiYP_401701.1. NC_007605.1.

3D structure databases

ProteinModelPortaliP03177.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi3783741.
KEGGivg:3783741.

Enzyme and pathway databases

SABIO-RKP03177.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR001889. Herpes_TK.
IPR013672. Herpes_TK_C.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00693. Herpes_TK. 1 hit.
PF08465. Herpes_TK_C. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence analysis of the 17,166 base-pair EcoRI fragment C of B95-8 Epstein-Barr virus."
    Bankier A.T., Deininger P.L., Farrell P.J., Barrell B.G.
    Mol. Biol. Med. 1:21-45(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "Identification of an Epstein-Barr virus-coded thymidine kinase."
    Littler E., Zeuthen J., McBride A.A., Soerensen E.T., Powell K.L., Walsh-Arrand J.E., Arrand J.R.
    EMBO J. 5:1959-1966(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION OF PROTEIN.
  4. Cited for: SUBCELLULAR LOCATION.
  5. "Epstein-Barr virus thymidine kinase is a centrosomal resident precisely localized to the periphery of centrioles."
    Gill M.B., Kutok J.L., Fingeroth J.D.
    J. Virol. 81:6523-6535(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.

Entry informationi

Entry nameiKITH_EBVB9
AccessioniPrimary (citable) accession number: P03177
Secondary accession number(s): Q777B9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: November 1, 1995
Last modified: April 1, 2015
This is version 76 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

Phosphorylates and thereby activates certain drugs like acyclovir (ACV), valacyclovir, and famciclovir to a toxic form, that leads to successful suppression of the infection, while the uninfected cell does not have this ability because it lacks TK.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.