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P03177

- KITH_EBVB9

UniProt

P03177 - KITH_EBVB9

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Protein
Thymidine kinase
Gene
TK, BXLF1
Organism
Epstein-Barr virus (strain B95-8) (HHV-4) (Human herpesvirus 4)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

In latent infection, may allow the virus to be reactivated and to grow in cells lacking a high concentration of phosphorylated nucleic acid precursors, such as memory B-cells that do not replicate their genome. Catalyzes the transfer of the gamma-phospho group of ATP to thymidine to generate dTMP in the salvage pathway of pyrimidine synthesis. The dTMP serves as a substrate for DNA polymerase during DNA replication By similarity.

Catalytic activityi

ATP + thymidine = ADP + thymidine 5'-phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei317 – 3171Proton acceptor Reviewed prediction
Binding sitei355 – 3551Substrate By similarity
Binding sitei402 – 4021Substrate By similarity
Binding sitei445 – 4451ATP By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi291 – 2988ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. thymidine kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA replication Source: UniProtKB-KW
  2. TMP biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

DNA synthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

SABIO-RKP03177.

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidine kinase (EC:2.7.1.21)
Gene namesi
Name:TK
ORF Names:BXLF1
OrganismiEpstein-Barr virus (strain B95-8) (HHV-4) (Human herpesvirus 4)
Taxonomic identifieri10377 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeGammaherpesvirinaeLymphocryptovirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
ProteomesiUP000007640: Genome

Subcellular locationi

Virion tegument. Host nucleus
Note: Localizes to the centrosome and more precisely to the periphery of the centriole, tightly encircling the tubulin-rich centrioles.2 Publications

GO - Cellular componenti

  1. host cell nucleus Source: UniProtKB-SubCell
  2. viral tegument Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Host nucleus, Virion, Virion tegument

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 607607Thymidine kinase
PRO_0000175059Add
BLAST

Expressioni

Keywords - Developmental stagei

Early protein

Interactioni

Subunit structurei

Homodimer By similarity.

Structurei

3D structure databases

ProteinModelPortaliP03177.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR001889. Herpes_TK.
IPR013672. Herpes_TK_C.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00693. Herpes_TK. 1 hit.
PF08465. Herpes_TK_C. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

P03177-1 [UniParc]FASTAAdd to Basket

« Hide

MAGFPGKEAG PPGGWRKCQE DESPENERHE NFYAEIDDFA PSVLTPTGSD    50
SGAGEEDDDG LYQVPTHWPP LMAPTGLSGE RVPCRTQAAV TSNTGNSPGS 100
RHTSCPFTLP RGAQPPAPAH QKPTAPTPKP RSRECGPSKT PDPFSWFRKT 150
SCTEGGADST SRSFMYQKGF EEGLAGLGLD DKSDCESEDE SNFRRPSSHS 200
ALKQKNGGKG KPSGLFEHLA AHGREFSKLS KHAAQLKRLS GSVMNVLNLD 250
DAQDTRQAKA QRKESMRVPI VTHLTNHVPV IKPACSLFLE GAPGVGKTTM 300
LNHLKAVFGD LTIVVPEPMR YWTHVYENAI KAMHKNVTRA RHGREDTSAE 350
VLACQMKFTT PFRVLASRKR SLLVTESGAR SVAPLDCWIL HDRHLLSASV 400
VFPLMLLRSQ LLSYSDFIQV LATFTADPGD TIVWMKLNVE ENMRRLKKRG 450
RKHESGLDAG YLKSVNDAYH AVYCAWLLTQ YFAPEDIVKV CAGLTTITTV 500
CHQSHTPIIR SGVAEKLYKN SIFSVLKEVI QPFRADAVLL EVCLAFTRTL 550
AYLQFVLVDL SEFQDDLPGC WTEIYMQALK NPAIRSQFFD WAGLSKVISD 600
FERGNRD 607
Length:607
Mass (Da):67,193
Last modified:November 1, 1995 - v2
Checksum:i97A4CCDB598A09F1
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
V01555 Genomic DNA. Translation: CAA24799.1.
AJ507799 Genomic DNA. Translation: CAD53451.1.
PIRiA00615. KIBETE.
RefSeqiYP_401701.1. NC_007605.1.

Genome annotation databases

GeneIDi3783741.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
V01555 Genomic DNA. Translation: CAA24799.1 .
AJ507799 Genomic DNA. Translation: CAD53451.1 .
PIRi A00615. KIBETE.
RefSeqi YP_401701.1. NC_007605.1.

3D structure databases

ProteinModelPortali P03177.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 3783741.

Enzyme and pathway databases

SABIO-RK P03177.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
InterProi IPR001889. Herpes_TK.
IPR013672. Herpes_TK_C.
IPR027417. P-loop_NTPase.
[Graphical view ]
Pfami PF00693. Herpes_TK. 1 hit.
PF08465. Herpes_TK_C. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence analysis of the 17,166 base-pair EcoRI fragment C of B95-8 Epstein-Barr virus."
    Bankier A.T., Deininger P.L., Farrell P.J., Barrell B.G.
    Mol. Biol. Med. 1:21-45(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "Identification of an Epstein-Barr virus-coded thymidine kinase."
    Littler E., Zeuthen J., McBride A.A., Soerensen E.T., Powell K.L., Walsh-Arrand J.E., Arrand J.R.
    EMBO J. 5:1959-1966(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION OF PROTEIN.
  4. Cited for: SUBCELLULAR LOCATION.
  5. "Epstein-Barr virus thymidine kinase is a centrosomal resident precisely localized to the periphery of centrioles."
    Gill M.B., Kutok J.L., Fingeroth J.D.
    J. Virol. 81:6523-6535(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.

Entry informationi

Entry nameiKITH_EBVB9
AccessioniPrimary (citable) accession number: P03177
Secondary accession number(s): Q777B9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: November 1, 1995
Last modified: May 14, 2014
This is version 72 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

Phosphorylates and thereby activates certain drugs like acyclovir (ACV), valacyclovir, and famciclovir to a toxic form, that leads to successful suppression of the infection, while the uninfected cell does not have this ability because it lacks TK.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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