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Protein

ICP47 protein

Gene

US12

Organism
Human herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in the inhibition of host immune response. Binds specifically to transporters associated with antigen processing (TAP), thereby blocking peptide-binding and translocation by TAP as well as subsequent loading of peptides onto MHC class I molecules. Empty MHC I molecules are retained in the endoplasmic reticulum and ultimately directed to proteasomal degradation. In consequence, infected cells are masked for immune recognition by cytotoxic T-lymphocytes.4 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei22 – 221Binding to TAP1 subunitBy similarity

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Host-virus interaction, Inhibition of host adaptive immune response by virus, Inhibition of host TAP by virus, Viral immunoevasion

Protein family/group databases

TCDBi8.A.72.1.1. the immune evasion protein, icp47 (icp47) family.

Names & Taxonomyi

Protein namesi
Recommended name:
ICP47 protein
Alternative name(s):
Immediate-early protein IE12
Immediate-early-5
Infected cell protein 47
US12 protein
Vmw12
Gene namesi
Name:US12
OrganismiHuman herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1)
Taxonomic identifieri10299 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeSimplexvirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000009294 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Host nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi24 – 241D → G: Reduced ability to inhibit peptide-binding to TAP. 1 Publication
Mutagenesisi31 – 311K → G: Reduced ability to inhibit peptide-binding to TAP. 1 Publication
Mutagenesisi32 – 321R → G: Reduced ability to inhibit peptide-binding to TAP. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 8888ICP47 proteinPRO_0000115810Add
BLAST

Expressioni

Keywords - Developmental stagei

Early protein

Interactioni

Subunit structurei

Interacts with host TAP1 and TAP2; these interactions inhibit the loading of peptides onto MHC class I molecules.1 Publication

Structurei

Secondary structure

1
88
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi5 – 139Combined sources
Beta strandi16 – 183Combined sources
Helixi22 – 3110Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1QLONMR-A1-34[»]
ProteinModelPortaliP03170.
SMRiP03170. Positions 1-34.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP03170.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2 – 3534Active domainBy similarityAdd
BLAST

Domaini

The N-terminal active domain blocks peptide binding to and peptide transport by TAP.By similarity

Sequence similaritiesi

Belongs to the herpesviridae US12 family.Curated

Phylogenomic databases

KOiK19446.

Family and domain databases

InterProiIPR008026. Herpes_ICP47.
[Graphical view]
PfamiPF05363. Herpes_US12. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P03170-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSWALEMADT FLDTMRVGPR TYADVRDEIN KRGREDREAA RTAVHDPERP
60 70 80
LLRSPGLLPE IAPNASLGVA HRRTGGTVTD SPRNPVTR
Length:88
Mass (Da):9,793
Last modified:March 1, 2004 - v2
Checksum:iC5D2EA8C35006612
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti14 – 141T → N (PubMed:6278443).Curated
Sequence conflicti14 – 141T → N (PubMed:11117552).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02220 Genomic DNA. Translation: AAA45795.1.
L00036 Genomic DNA. Translation: AAA96676.1.
X00428 Genomic RNA. Translation: CAA25124.1.
X02138 Genomic DNA. Translation: CAA26066.1.
X14112 Genomic DNA. Translation: CAA32277.1.
V00462 Genomic DNA. Translation: CAA23737.1.
AF290017 Genomic DNA. Translation: AAG33133.1.
AF290018 Genomic DNA. Translation: AAG33134.1.
AF324428 Genomic DNA. Translation: AAK12110.1.
PIRiA93454. EDBE51.
RefSeqiYP_009137148.1. NC_001806.2.

Genome annotation databases

GeneIDi2703441.
KEGGivg:2703441.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02220 Genomic DNA. Translation: AAA45795.1.
L00036 Genomic DNA. Translation: AAA96676.1.
X00428 Genomic RNA. Translation: CAA25124.1.
X02138 Genomic DNA. Translation: CAA26066.1.
X14112 Genomic DNA. Translation: CAA32277.1.
V00462 Genomic DNA. Translation: CAA23737.1.
AF290017 Genomic DNA. Translation: AAG33133.1.
AF290018 Genomic DNA. Translation: AAG33134.1.
AF324428 Genomic DNA. Translation: AAK12110.1.
PIRiA93454. EDBE51.
RefSeqiYP_009137148.1. NC_001806.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1QLONMR-A1-34[»]
ProteinModelPortaliP03170.
SMRiP03170. Positions 1-34.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

TCDBi8.A.72.1.1. the immune evasion protein, icp47 (icp47) family.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi2703441.
KEGGivg:2703441.

Phylogenomic databases

KOiK19446.

Miscellaneous databases

EvolutionaryTraceiP03170.

Family and domain databases

InterProiIPR008026. Herpes_ICP47.
[Graphical view]
PfamiPF05363. Herpes_US12. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiICP47_HHV11
AccessioniPrimary (citable) accession number: P03170
Secondary accession number(s): Q99BW2, Q9E0N0, Q9E0N1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: March 1, 2004
Last modified: September 7, 2016
This is version 84 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.