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Protein

Capsid protein

Gene

C

Organism
Hepatitis B virus genotype D subtype adw (isolate United Kingdom/adyw/1979) (HBV-D)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Self assembles to form an icosahedral capsid. Most capsid appear to be large particles with a icosahedral symmetry of T=4 and consist of 240 copies of capsid protein, though a fraction forms smaller T=3 particles consisting of 180 capsid proteins. Entering capsid are transported along microtubules to the nucleus. Phosphorylation of the capsid is thought to induce exposure of nuclear localization signal in the C-terminal portion of the capsid protein that allows binding to the nuclear pore complex via the importin (karyopherin-) alpha and beta. Capsids are imported in intact form through the nuclear pore into the nuclear basket, where it probably binds NUP153. Only capsids that contain the mature viral genome can release the viral DNA and capsid protein into the nucleoplasm. Immature capsids get stucked in the basket. Capsids encapsulate the pre-genomic RNA and the P protein. Pre-genomic RNA is reverse transcribed into DNA while the capsid is still in the cytoplasm. The capsid can then either be directed to the nucleus, providing more genome for transcription, or bud through the endoplasmic reticulum to provide new virions (By similarity).By similarity
Encapsidates hepatitis delta genome.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cytoplasmic inwards viral transport, Host-virus interaction, Microtubular inwards viral transport, Viral penetration into host nucleus, Virus entry into host cell

Keywords - Ligandi

DNA-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Capsid protein
Alternative name(s):
Core antigen
Core protein
HBcAg
p21.5
Gene namesi
Name:C
OrganismiHepatitis B virus genotype D subtype adw (isolate United Kingdom/adyw/1979) (HBV-D)
Taxonomic identifieri10419 [NCBI]
Taxonomic lineageiVirusesRetro-transcribing virusesHepadnaviridaeOrthohepadnavirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Pan troglodytes (Chimpanzee) [TaxID: 9598]

Subcellular locationi

Capsid protein :
  • Virion By similarity
  • Host cytoplasm By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Capsid protein, Host cytoplasm, T=4 icosahedral capsid protein, Virion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi97 – 971F → L: Enhances capsid assembly. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 183183Capsid proteinPRO_0000222318Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei87 – 871Phosphoserine; by host PKABy similarity
Modified residuei155 – 1551Phosphoserine; by hostBy similarity
Modified residuei162 – 1621Phosphoserine; by hostBy similarity
Modified residuei170 – 1701Phosphoserine; by hostBy similarity

Post-translational modificationi

Phosphorylated by host SRPK1, SRPK2, and maybe protein kinase A, protein kinase C or GAPDH. Phosphorylation is critical for pregenomic RNA packaging. Protein kinase C phosphorylation is stimulated by HBx protein and may play a role in transport of the viral genome to the nucleus at the late step during viral replication cycle. Protein kinase A phosphorylation may promote capsid assembly (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Homodimerizes, then multimerizes. Interacts with cytosol exposed regions of viral L glycoprotein present in the reticulum-to-Golgi compartment. Interacts with human FLNB (By similarity).By similarity

Protein-protein interaction databases

DIPiDIP-60476N.

Structurei

Secondary structure

1
183
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi7 – 93Combined sources
Helixi13 – 164Combined sources
Helixi21 – 233Combined sources
Helixi27 – 4216Combined sources
Beta strandi44 – 463Combined sources
Helixi50 – 7526Combined sources
Helixi79 – 9012Combined sources
Helixi92 – 11019Combined sources
Helixi112 – 12615Combined sources
Helixi130 – 1323Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2G33X-ray3.96A/B/C/D1-149[»]
2G34X-ray5.05A/B/C/D1-149[»]
2QIJX-ray8.90A/B/C/D3-148[»]
3KXSX-ray2.25A/B/C/D/E/F1-143[»]
4BMGX-ray3.00A/B/C/D/E/F1-149[»]
4G93X-ray4.20A/B/C/D1-149[»]
5D7YX-ray3.89A/B/C/D1-149[»]
5E0IX-ray1.95A/B/C/D/E/F1-149[»]
ProteinModelPortaliP03147.
SMRiP03147. Positions 3-143.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP03147.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati155 – 16171; half-length
Repeati162 – 16982
Repeati170 – 17783

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni155 – 177233 X 8 AA repeats of S-P-R-R-R-[PR]-S-QAdd
BLAST
Regioni177 – 1837RNA bindingBy similarity

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi158 – 17518Bipartite nuclear localization signalSequence analysisAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Repeat

Family and domain databases

InterProiIPR002006. Viral_capsid_core_Hepatitis.
[Graphical view]
PfamiPF00906. Hepatitis_core. 3 hits.
[Graphical view]
SUPFAMiSSF47852. SSF47852. 1 hit.

Sequencei

Sequence statusi: Complete.

P03147-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDIDPYKEFG ATVELLSFLP SDFFPSVRDL LDTAAALYRD ALESPEHCSP
60 70 80 90 100
HHTALRQAIL CWGDLMTLAT WVGTNLEDPA SRDLVVSYVN TNVGLKFRQL
110 120 130 140 150
LWFHISCLTF GRETVLEYLV SFGVWIRTPP AYRPPNAPIL STLPETTVVR
160 170 180
RRGRSPRRRT PSPRRRRSQS PRRRRSQSRE SQC
Length:183
Mass (Da):21,042
Last modified:July 21, 1986 - v1
Checksum:i545ED0E55527F26C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02202 Genomic RNA. Translation: AAA45486.1.
PIRiB93217. NKVLA2.

Cross-referencesi

Web resourcesi

HepSEQ

Hepatitis virus B database

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02202 Genomic RNA. Translation: AAA45486.1.
PIRiB93217. NKVLA2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2G33X-ray3.96A/B/C/D1-149[»]
2G34X-ray5.05A/B/C/D1-149[»]
2QIJX-ray8.90A/B/C/D3-148[»]
3KXSX-ray2.25A/B/C/D/E/F1-143[»]
4BMGX-ray3.00A/B/C/D/E/F1-149[»]
4G93X-ray4.20A/B/C/D1-149[»]
5D7YX-ray3.89A/B/C/D1-149[»]
5E0IX-ray1.95A/B/C/D/E/F1-149[»]
ProteinModelPortaliP03147.
SMRiP03147. Positions 3-143.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-60476N.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP03147.

Family and domain databases

InterProiIPR002006. Viral_capsid_core_Hepatitis.
[Graphical view]
PfamiPF00906. Hepatitis_core. 3 hits.
[Graphical view]
SUPFAMiSSF47852. SSF47852. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCAPSD_HBVD1
AccessioniPrimary (citable) accession number: P03147
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: June 8, 2016
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.