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Protein

Capsid protein

Gene

C

Organism
Hepatitis B virus genotype D subtype adw (isolate United Kingdom/adyw/1979) (HBV-D)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Self assembles to form an icosahedral capsid. Most capsid appear to be large particles with a icosahedral symmetry of T=4 and consist of 240 copies of capsid protein, though a fraction forms smaller T=3 particles consisting of 180 capsid proteins. Entering capsid are transported along microtubules to the nucleus. Phosphorylation of the capsid is thought to induce exposure of nuclear localization signal in the C-terminal portion of the capsid protein that allows binding to the nuclear pore complex via the importin (karyopherin-) alpha and beta. Capsids are imported in intact form through the nuclear pore into the nuclear basket, where it probably binds NUP153. Only capsids that contain the mature viral genome can release the viral DNA and capsid protein into the nucleoplasm. Immature capsids get stucked in the basket. Capsids encapsulate the pre-genomic RNA and the P protein. Pre-genomic RNA is reverse transcribed into DNA while the capsid is still in the cytoplasm. The capsid can then either be directed to the nucleus, providing more genome for transcription, or bud through the endoplasmic reticulum to provide new virions.UniRule annotation
Encapsidates hepatitis delta genome.UniRule annotation

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • identical protein binding Source: IntAct
  • RNA binding Source: UniProtKB-KW
  • structural molecule activity Source: InterPro

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, RNA-binding
Biological processCytoplasmic inwards viral transport, Host-virus interaction, Microtubular inwards viral transport, Viral penetration into host nucleus, Virus entry into host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Capsid proteinUniRule annotation
Alternative name(s):
Core antigenUniRule annotation
Core proteinUniRule annotation
HBcAgUniRule annotation
p21.5UniRule annotation
Gene namesi
Name:CUniRule annotation
OrganismiHepatitis B virus genotype D subtype adw (isolate United Kingdom/adyw/1979) (HBV-D)
Taxonomic identifieri10419 [NCBI]
Taxonomic lineageiVirusesRetro-transcribing virusesHepadnaviridaeOrthohepadnavirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Pan troglodytes (Chimpanzee) [TaxID: 9598]

Subcellular locationi

  • Virion UniRule annotation
  • Host cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Capsid protein, Host cytoplasm, T=4 icosahedral capsid protein, Virion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi97F → L: Enhances capsid assembly. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002223181 – 183Capsid proteinAdd BLAST183

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei155Phosphoserine; by hostUniRule annotation1
Modified residuei162Phosphoserine; by hostUniRule annotation1
Modified residuei170Phosphoserine; by hostUniRule annotation1

Post-translational modificationi

Phosphorylated by host SRPK1, SRPK2, and maybe protein kinase C or GAPDH. Phosphorylation is critical for pregenomic RNA packaging. Protein kinase C phosphorylation is stimulated by HBx protein and may play a role in transport of the viral genome to the nucleus at the late step during viral replication cycle.UniRule annotation

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP03147

Interactioni

Subunit structurei

Homodimerizes, then multimerizes. Interacts with cytosol exposed regions of viral L glycoprotein present in the reticulum-to-Golgi compartment. Interacts with human FLNB. Phosphorylated form interacts with host importin alpha; this interaction depends on the exposure of the NLS, which itself depends upon genome maturation and/or phosphorylation of the capsid protein. Interacts with host NUP153.UniRule annotation

Binary interactionsi

WithEntry#Exp.IntActNotes
itself7EBI-15711833,EBI-15711833

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

DIPiDIP-60476N

Structurei

Secondary structure

1183
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi7 – 9Combined sources3
Helixi13 – 16Combined sources4
Helixi21 – 23Combined sources3
Helixi27 – 43Combined sources17
Beta strandi44 – 46Combined sources3
Helixi50 – 74Combined sources25
Helixi79 – 91Combined sources13
Helixi93 – 110Combined sources18
Helixi112 – 126Combined sources15
Helixi130 – 132Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2G33X-ray3.96A/B/C/D1-149[»]
2G34X-ray5.05A/B/C/D1-149[»]
2QIJX-ray8.90A/B/C/D3-148[»]
3KXSX-ray2.25A/B/C/D/E/F1-143[»]
4BMGX-ray3.00A/B/C/D/E/F1-149[»]
4G93X-ray4.20A/B/C/D1-149[»]
5D7YX-ray3.89A/B/C/D1-149[»]
5E0IX-ray1.95A/B/C/D/E/F1-149[»]
5GMZX-ray1.70A/B/C/D/E/F1-149[»]
5WTWX-ray2.62A/B1-142[»]
6BVFelectron microscopy4.00A/B/C/D1-149[»]
6BVNelectron microscopy4.00A/B/C1-149[»]
ProteinModelPortaliP03147
SMRiP03147
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP03147

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati155 – 1611; half-length7
Repeati162 – 16928
Repeati170 – 17738

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni155 – 1773 X 8 AA repeats of S-P-R-R-R-[PR]-S-QAdd BLAST23
Regioni177 – 183RNA bindingUniRule annotation7

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi158 – 175Bipartite nuclear localization signalUniRule annotationAdd BLAST18

Sequence similaritiesi

Belongs to the orthohepadnavirus core antigen family.UniRule annotation

Keywords - Domaini

Repeat

Family and domain databases

Gene3Di1.10.4090.10, 1 hit
HAMAPiMF_04076 HBV_HBEAG, 1 hit
InterProiView protein in InterPro
IPR002006 Hepatitis_core
IPR036459 Viral_capsid_core_dom_sf_HBV
PfamiView protein in Pfam
PF00906 Hepatitis_core, 3 hits
SUPFAMiSSF47852 SSF47852, 1 hit

Sequencei

Sequence statusi: Complete.

P03147-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDIDPYKEFG ATVELLSFLP SDFFPSVRDL LDTAAALYRD ALESPEHCSP
60 70 80 90 100
HHTALRQAIL CWGDLMTLAT WVGTNLEDPA SRDLVVSYVN TNVGLKFRQL
110 120 130 140 150
LWFHISCLTF GRETVLEYLV SFGVWIRTPP AYRPPNAPIL STLPETTVVR
160 170 180
RRGRSPRRRT PSPRRRRSQS PRRRRSQSRE SQC
Length:183
Mass (Da):21,042
Last modified:July 21, 1986 - v1
Checksum:i545ED0E55527F26C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02202 Genomic RNA Translation: AAA45486.1
PIRiB93217 NKVLA2

Similar proteinsi

Entry informationi

Entry nameiCAPSD_HBVD1
AccessioniPrimary (citable) accession number: P03147
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: May 23, 2018
This is version 102 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

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