P03134 (VNCS_MUMIP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Non-capsid protein NS-1

Alternative name(s):
NCVP1
Non-structural protein NS1

Gene names

Name:

NS1

Organism

Murine minute virus (strain MVM prototype) (MVM) (Murine minute virus (strain MVM(p)))

Taxonomic identifier

648235 [NCBI]

Taxonomic lineage

Viruses › ssDNA viruses › Parvoviridae › Parvovirinae › Parvovirus

Virus host

Mus musculus (Mouse) [TaxID: 10090]

Protein attributes

Sequence length	672 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Seems necessary for viral DNA replication.
Subunit structure	Forms homooligomers. Interacts with human SYNCRIP. Ref.2
Domain	The N-terminus (residues 1-275) possess a negative effect on transactivation. The C-terminus (residues 543-672) possess an activation domain.
Sequence similarities	Belongs to the parvoviruses non-capsid protein family. Contains 1 SF3 helicase domain.
Sequence caution	The sequence CAA24309.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	DNA replication Host-virus interaction Initiation of viral infection Viral penetration into host cytoplasm Viral penetration via permeabilization of host organellar membrane
Ligand	ATP-binding Nucleotide-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW interspecies interaction between organisms Inferred from electronic annotation. Source: UniProtKB-KW viral genome replication Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 672	672	Non-capsid protein NS-1		PRO_0000222466
Regions
	Domain	373 – 528	156	SF3 helicase
	Nucleotide binding	399 – 406	8	ATP Potential
	Region	1 – 276	276	Interacts with SYNCRIP

Sequences

	Sequence		Length	Mass (Da)
	P03134 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: 50298F27662E3C1D	FASTA	672	76,249
	10 20 30 40 50 60 MAGNAYSDEV LGATNWLKEK SNQEVFSFVF KNENVQLNGK DIGWNSYKKE LQEDELKSLQ 70 80 90 100 110 120 RGAETTWDQS EDMEWETTVD EMTKKQVFIF DSLVKKCLFE VLNTKNIFPG DVNWFVQHEW 130 140 150 160 170 180 GKDQGWHCHV LIGGKDFSQA QGKWWRRQLN VYWSRWLVTA CNVQLTPAER IKLREIAEDN 190 200 210 220 230 240 EWVTLLTYKH KQTKKDYTKC VLFGNMIAYY FLTKKKISTS PPRDGGYFLS SDSGWKTNFL 250 260 270 280 290 300 KEGERHLVSK LYTDDMRPET VETTVTTAQE TKRGRIQTKK EVSIKTTLKE LVHKRVTSPE 310 320 330 340 350 360 DWMMMQPDSY IEMMAQPGGE NLLKNTLEIC TLTLARTKTA FDLILEKAET SKLTNFSLPD 370 380 390 400 410 420 TRTCRIFAFH GWNYVKVCHA ICCVLNRQGG KRNTVLFHGP ASTGKSIIAQ AIAQAVGNVG 430 440 450 460 470 480 CYNAANVNFP FNDCTNKNLI WVEEAGNFGQ QVNQFKAICS GQTIRIDQKG KGSKQIEPTP 490 500 510 520 530 540 VIMTTNENIT VVRIGCEERP EHTQPIRDRM LNIHLTHTLP GDFGLVDKNE WPMICAWLVK 550 560 570 580 590 600 NGYQSTMASY CAKWGKVPDW SENWAEPKVP TPINLLGSAR SPFTTPKSTP LSQNYALTPL 610 620 630 640 650 660 ASDLEDLALE PWSTPNTPVA GTAETQNTGE AGSKACQDGQ LSPTWSEIEE DLRACFGAEP 670 LKKDFSEPLN LD

References

[1]	"The complete DNA sequence of minute virus of mice, an autonomous parvovirus." Astell C.R., Thomson M., Merchlinsky M., Ward D.C. Nucleic Acids Res. 11:999-1018(1983) [PubMed: 6298737] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"A novel heterogeneous nuclear ribonucleoprotein-like protein interacts with NS1 of the minute virus of mice." Harris C.E., Boden R.A., Astell C.R. J. Virol. 73:72-80(1999) [PubMed: 9847309] [Abstract] Cited for: HOMOOLIGOMERIZATION, INTERACTION WITH SYNCRIP.

Cross-references

Sequence databases
EMBL GenBank DDBJ	J02275 Genomic DNA. Translation: AAA67109.1. V01115 Genomic DNA. Translation: CAA24309.1. Different initiation.
PIR	UYPV1M. A03696.
RefSeq	NP_041243.1. NC_001510.1.
3D structure databases
ProteinModelPortal	P03134.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-46505N.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1489590.
Family and domain databases
InterPro	IPR014015. Helicase_SF3_DNA-vir. IPR021972. Parvovirus_NS1_C. IPR001257. Parvovirus_NS1_helicase. IPR021076. Parvovirus_NS1_N. [Graphical view]
Pfam	PF12117. DUF3580. 1 hit. PF01057. Parvo_NS1. 1 hit. PF12433. PV_NSP1. 1 hit. [Graphical view]
PROSITE	PS51206. SF3_HELICASE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

VNCS_MUMIP

Accession

Primary (citable) accession number: P03134

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	September 21, 2011
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program