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Protein

Non-structural protein NS1

Gene

NS1

Organism
Murine minute virus (strain MVM prototype) (MVM) (Murine minute virus (strain MVM(p)))
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Multifunctional protein that plays an essential role in viral DNA replication. Inhibits the host cell cycle during the G1/S transition, the S-phase, and the G2/M transition. These arrests may provide essential cellular factors for viral DNA replication. Interacts specifically with the viral DNA origin of replication and plays a direct role in DNA replication. Participates in the transcriptional regulation of several promoters including the viral p38 promoter that regulates the expression of VP1 and VP2 transcripts. Promotes apoptosis in host cell.4 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi399 – 4068ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

DNA replication, G1/S host cell cycle checkpoint dysregulation by virus, Host G2/M cell cycle arrest by virus, Host-virus interaction, Modulation of host cell apoptosis by virus, Modulation of host cell cycle by virus

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Non-structural protein NS1
Gene namesi
Name:NS1
OrganismiMurine minute virus (strain MVM prototype) (MVM) (Murine minute virus (strain MVM(p)))
Taxonomic identifieri648235 [NCBI]
Taxonomic lineageiVirusesssDNA virusesParvoviridaeParvovirinaeProtoparvovirus
Virus hostiMus musculus (Mouse) [TaxID: 10090]
Proteomesi
  • UP000007019 Componenti: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 672672Non-structural protein NS1PRO_0000222466Add
BLAST

Post-translational modificationi

Phosphorylated.1 Publication

Interactioni

Subunit structurei

Forms homooligomers. Interacts with human SYNCRIP.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Smn1P978013EBI-9515229,EBI-309807From a different organism.

Protein-protein interaction databases

DIPiDIP-46505N.
IntActiP03134. 2 interactions.

Structurei

Secondary structure

1
672
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi8 – 1912Combined sources
Turni20 – 223Combined sources
Beta strandi24 – 318Combined sources
Beta strandi35 – 373Combined sources
Beta strandi40 – 423Combined sources
Helixi54 – 7017Combined sources
Beta strandi73 – 753Combined sources
Helixi81 – 10424Combined sources
Helixi109 – 1113Combined sources
Beta strandi112 – 12110Combined sources
Turni122 – 1243Combined sources
Beta strandi125 – 13410Combined sources
Helixi139 – 1413Combined sources
Helixi142 – 16019Combined sources
Helixi167 – 17913Combined sources
Beta strandi182 – 1854Combined sources
Beta strandi192 – 1943Combined sources
Helixi203 – 2097Combined sources
Helixi211 – 2133Combined sources
Beta strandi219 – 2213Combined sources
Beta strandi227 – 2326Combined sources
Beta strandi235 – 2373Combined sources
Helixi242 – 25211Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3WRNX-ray1.52A1-255[»]
3WROX-ray1.48A1-255[»]
3WRQX-ray1.53A1-255[»]
3WRRX-ray1.62A1-255[»]
3WRSX-ray1.58A1-255[»]
4PP4X-ray1.45A1-255[»]
4R94X-ray1.67A1-255[»]
ProteinModelPortaliP03134.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini373 – 528156SF3 helicasePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 276276Interacts with SYNCRIPAdd
BLAST

Domaini

The N-terminus (residues 1-275) possesses a negative effect on transactivation.
The C-terminus (residues 543-672) possesses an activation domain.

Sequence similaritiesi

Contains 1 SF3 helicase domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR014015. Helicase_SF3_DNA-vir.
IPR027417. P-loop_NTPase.
IPR021972. Parvovirus_NS1_C.
IPR001257. Parvovirus_NS1_helicase.
IPR021076. Parvovirus_NS1_N.
[Graphical view]
PfamiPF12117. DUF3580. 1 hit.
PF01057. Parvo_NS1. 1 hit.
PF12433. PV_NSP1. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS51206. SF3_HELICASE_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P03134-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGNAYSDEV LGATNWLKEK SNQEVFSFVF KNENVQLNGK DIGWNSYKKE
60 70 80 90 100
LQEDELKSLQ RGAETTWDQS EDMEWETTVD EMTKKQVFIF DSLVKKCLFE
110 120 130 140 150
VLNTKNIFPG DVNWFVQHEW GKDQGWHCHV LIGGKDFSQA QGKWWRRQLN
160 170 180 190 200
VYWSRWLVTA CNVQLTPAER IKLREIAEDN EWVTLLTYKH KQTKKDYTKC
210 220 230 240 250
VLFGNMIAYY FLTKKKISTS PPRDGGYFLS SDSGWKTNFL KEGERHLVSK
260 270 280 290 300
LYTDDMRPET VETTVTTAQE TKRGRIQTKK EVSIKTTLKE LVHKRVTSPE
310 320 330 340 350
DWMMMQPDSY IEMMAQPGGE NLLKNTLEIC TLTLARTKTA FDLILEKAET
360 370 380 390 400
SKLTNFSLPD TRTCRIFAFH GWNYVKVCHA ICCVLNRQGG KRNTVLFHGP
410 420 430 440 450
ASTGKSIIAQ AIAQAVGNVG CYNAANVNFP FNDCTNKNLI WVEEAGNFGQ
460 470 480 490 500
QVNQFKAICS GQTIRIDQKG KGSKQIEPTP VIMTTNENIT VVRIGCEERP
510 520 530 540 550
EHTQPIRDRM LNIHLTHTLP GDFGLVDKNE WPMICAWLVK NGYQSTMASY
560 570 580 590 600
CAKWGKVPDW SENWAEPKVP TPINLLGSAR SPFTTPKSTP LSQNYALTPL
610 620 630 640 650
ASDLEDLALE PWSTPNTPVA GTAETQNTGE AGSKACQDGQ LSPTWSEIEE
660 670
DLRACFGAEP LKKDFSEPLN LD
Length:672
Mass (Da):76,249
Last modified:July 21, 1986 - v1
Checksum:i50298F27662E3C1D
GO

Sequence cautioni

The sequence CAA24309 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02275 Genomic DNA. Translation: AAA67109.1.
V01115 Genomic DNA. Translation: CAA24309.1. Different initiation.
PIRiA03696. UYPV1M.
RefSeqiNP_041242.1. NC_001510.1.
NP_041243.1. NC_001510.1.

Genome annotation databases

GeneIDi1489590.
KEGGivg:1489590.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02275 Genomic DNA. Translation: AAA67109.1.
V01115 Genomic DNA. Translation: CAA24309.1. Different initiation.
PIRiA03696. UYPV1M.
RefSeqiNP_041242.1. NC_001510.1.
NP_041243.1. NC_001510.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3WRNX-ray1.52A1-255[»]
3WROX-ray1.48A1-255[»]
3WRQX-ray1.53A1-255[»]
3WRRX-ray1.62A1-255[»]
3WRSX-ray1.58A1-255[»]
4PP4X-ray1.45A1-255[»]
4R94X-ray1.67A1-255[»]
ProteinModelPortaliP03134.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-46505N.
IntActiP03134. 2 interactions.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1489590.
KEGGivg:1489590.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR014015. Helicase_SF3_DNA-vir.
IPR027417. P-loop_NTPase.
IPR021972. Parvovirus_NS1_C.
IPR001257. Parvovirus_NS1_helicase.
IPR021076. Parvovirus_NS1_N.
[Graphical view]
PfamiPF12117. DUF3580. 1 hit.
PF01057. Parvo_NS1. 1 hit.
PF12433. PV_NSP1. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS51206. SF3_HELICASE_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNS1_MUMIP
AccessioniPrimary (citable) accession number: P03134
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: June 8, 2016
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.