Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Non-structural protein NS1

Gene

NS1

Organism
Murine minute virus (strain MVM prototype) (MVM) (Murine minute virus (strain MVM(p)))
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Multifunctional protein that plays an essential role in viral DNA replication. Inhibits the host cell cycle during the G1/S transition, the S-phase, and the G2/M transition. These arrests may provide essential cellular factors for viral DNA replication. Interacts specifically with the viral DNA origin of replication and plays a direct role in DNA replication. Participates in the transcriptional regulation of several promoters including the viral p38 promoter that regulates the expression of VP1 and VP2 transcripts. Promotes apoptosis in host cell.4 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi399 – 406ATPPROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding
Biological processDNA replication, G1/S host cell cycle checkpoint dysregulation by virus, Host G2/M cell cycle arrest by virus, Host-virus interaction, Modulation of host cell apoptosis by virus, Modulation of host cell cycle by virus
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Non-structural protein NS1
Gene namesi
Name:NS1
OrganismiMurine minute virus (strain MVM prototype) (MVM) (Murine minute virus (strain MVM(p)))
Taxonomic identifieri648235 [NCBI]
Taxonomic lineageiVirusesssDNA virusesParvoviridaeParvovirinaeProtoparvovirus
Virus hostiMus musculus (Mouse) [TaxID: 10090]
Proteomesi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002224661 – 672Non-structural protein NS1Add BLAST672

Post-translational modificationi

Phosphorylated.1 Publication

Proteomic databases

PRIDEiP03134

Interactioni

Subunit structurei

Forms homooligomers. Interacts with human SYNCRIP.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Smn1P978013EBI-9515229,EBI-309807From Mus musculus.

Protein-protein interaction databases

DIPiDIP-46505N
IntActiP03134, 5 interactors

Structurei

Secondary structure

1672
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi8 – 19Combined sources12
Turni20 – 22Combined sources3
Beta strandi24 – 31Combined sources8
Beta strandi35 – 37Combined sources3
Beta strandi40 – 42Combined sources3
Helixi54 – 70Combined sources17
Beta strandi73 – 75Combined sources3
Helixi81 – 104Combined sources24
Helixi109 – 111Combined sources3
Beta strandi112 – 121Combined sources10
Turni122 – 124Combined sources3
Beta strandi125 – 134Combined sources10
Helixi139 – 141Combined sources3
Helixi142 – 160Combined sources19
Helixi167 – 179Combined sources13
Beta strandi182 – 185Combined sources4
Beta strandi192 – 194Combined sources3
Helixi203 – 209Combined sources7
Helixi211 – 213Combined sources3
Beta strandi219 – 221Combined sources3
Beta strandi227 – 232Combined sources6
Beta strandi235 – 237Combined sources3
Helixi242 – 252Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3WRNX-ray1.52A1-255[»]
3WROX-ray1.48A1-255[»]
3WRQX-ray1.53A1-255[»]
3WRRX-ray1.62A1-255[»]
3WRSX-ray1.58A1-255[»]
4PP4X-ray1.45A1-255[»]
4R94X-ray1.67A1-255[»]
ProteinModelPortaliP03134
SMRiP03134
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini373 – 528SF3 helicasePROSITE-ProRule annotationAdd BLAST156

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 276Interaction with SYNCRIP1 PublicationAdd BLAST276

Domaini

The N-terminus (residues 1-275) possesses a negative effect on transactivation.
The C-terminus (residues 543-672) possesses an activation domain.

Sequence similaritiesi

Phylogenomic databases

OrthoDBiVOG09000052

Family and domain databases

InterProiView protein in InterPro
IPR014015 Helicase_SF3_DNA-vir
IPR027417 P-loop_NTPase
IPR021972 Parvovirus_NS1_C
IPR001257 Parvovirus_NS1_helicase
IPR021076 Parvovirus_NS1_N
PfamiView protein in Pfam
PF12117 DUF3580, 1 hit
PF01057 Parvo_NS1, 1 hit
PF12433 PV_NSP1, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS51206 SF3_HELICASE_1, 1 hit

Sequencei

Sequence statusi: Complete.

P03134-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGNAYSDEV LGATNWLKEK SNQEVFSFVF KNENVQLNGK DIGWNSYKKE
60 70 80 90 100
LQEDELKSLQ RGAETTWDQS EDMEWETTVD EMTKKQVFIF DSLVKKCLFE
110 120 130 140 150
VLNTKNIFPG DVNWFVQHEW GKDQGWHCHV LIGGKDFSQA QGKWWRRQLN
160 170 180 190 200
VYWSRWLVTA CNVQLTPAER IKLREIAEDN EWVTLLTYKH KQTKKDYTKC
210 220 230 240 250
VLFGNMIAYY FLTKKKISTS PPRDGGYFLS SDSGWKTNFL KEGERHLVSK
260 270 280 290 300
LYTDDMRPET VETTVTTAQE TKRGRIQTKK EVSIKTTLKE LVHKRVTSPE
310 320 330 340 350
DWMMMQPDSY IEMMAQPGGE NLLKNTLEIC TLTLARTKTA FDLILEKAET
360 370 380 390 400
SKLTNFSLPD TRTCRIFAFH GWNYVKVCHA ICCVLNRQGG KRNTVLFHGP
410 420 430 440 450
ASTGKSIIAQ AIAQAVGNVG CYNAANVNFP FNDCTNKNLI WVEEAGNFGQ
460 470 480 490 500
QVNQFKAICS GQTIRIDQKG KGSKQIEPTP VIMTTNENIT VVRIGCEERP
510 520 530 540 550
EHTQPIRDRM LNIHLTHTLP GDFGLVDKNE WPMICAWLVK NGYQSTMASY
560 570 580 590 600
CAKWGKVPDW SENWAEPKVP TPINLLGSAR SPFTTPKSTP LSQNYALTPL
610 620 630 640 650
ASDLEDLALE PWSTPNTPVA GTAETQNTGE AGSKACQDGQ LSPTWSEIEE
660 670
DLRACFGAEP LKKDFSEPLN LD
Length:672
Mass (Da):76,249
Last modified:July 21, 1986 - v1
Checksum:i50298F27662E3C1D
GO

Sequence cautioni

The sequence CAA24309 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02275 Genomic DNA Translation: AAA67109.1
V01115 Genomic DNA Translation: CAA24309.1 Different initiation.
PIRiA03696 UYPV1M
RefSeqiNP_041242.1, NC_001510.1
NP_041243.1, NC_001510.1

Genome annotation databases

GeneIDi1489590
KEGGivg:1489590

Entry informationi

Entry nameiNS1_MUMIP
AccessioniPrimary (citable) accession number: P03134
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: May 23, 2018
This is version 96 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health