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Protein

Protein Rep68

Gene

Rep68

Organism
Adeno-associated virus 2 (isolate Srivastava/1982) (AAV-2)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Plays an essential role in the initiation of viral DNA synthesis. Binds specifically to an inverted terminal repeat element (ITR) on the 3' and 5' ends of the viral DNA, where it cleaves a site specifically to generate a priming site for initiation of the synthesis of a complementary strand. Plays also a role as transcriptional regulator, DNA helicase and as key factor in site-specific integration of the viral genome. Inhibits the host cell cycle G1/S and G2/M transitions. These arrests may provide essential cellular factors for viral DNA replication.1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi334 – 341ATPPROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

DNA replication, G1/S host cell cycle checkpoint dysregulation by virus, Host G2/M cell cycle arrest by virus, Host-virus interaction, Modulation of host cell cycle by virus, Viral penetration into host cytoplasm, Viral penetration via permeabilization of host membrane, Virus entry into host cell

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Protein Rep68 (EC:3.6.4.12)
Gene namesi
Name:Rep68
OrganismiAdeno-associated virus 2 (isolate Srivastava/1982) (AAV-2)
Taxonomic identifieri648242 [NCBI]
Taxonomic lineageiVirusesssDNA virusesParvoviridaeParvovirinaeDependoparvovirus
Virus hostiMammalia [TaxID: 40674]
Proteomesi
  • UP000008469 Componenti: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002224741 – 536Protein Rep68Add BLAST536

Interactioni

Subunit structurei

Interacts with host TOPORS. Interacts with host KCTD5.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
E2P031202EBI-7387242,EBI-1779322From a different organism.
KCTD5Q9NXV23EBI-7387242,EBI-1056857From a different organism.
SUB1P539993EBI-7387242,EBI-998260From a different organism.
TOPORSQ9NS563EBI-7387242,EBI-1996473From a different organism.

Protein-protein interaction databases

IntActiP03132. 4 interactors.
MINTiMINT-6732280.

Structurei

Secondary structure

1536
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 11Combined sources8
Helixi15 – 18Combined sources4
Helixi24 – 32Combined sources9
Helixi45 – 47Combined sources3
Helixi50 – 71Combined sources22
Beta strandi78 – 84Combined sources7
Beta strandi89 – 96Combined sources8
Helixi102 – 104Combined sources3
Helixi105 – 119Combined sources15
Turni120 – 123Combined sources4
Beta strandi132 – 134Combined sources3
Beta strandi136 – 140Combined sources5
Helixi152 – 156Combined sources5
Turni157 – 159Combined sources3
Turni162 – 164Combined sources3
Beta strandi165 – 170Combined sources6
Helixi173 – 175Combined sources3
Turni176 – 180Combined sources5
Helixi182 – 204Combined sources23
Helixi225 – 234Combined sources10
Helixi239 – 254Combined sources16
Helixi260 – 277Combined sources18
Helixi280 – 284Combined sources5
Helixi293 – 295Combined sources3
Helixi297 – 304Combined sources8
Helixi309 – 320Combined sources12
Turni322 – 326Combined sources5
Beta strandi329 – 333Combined sources5
Helixi340 – 350Combined sources11
Beta strandi354 – 356Combined sources3
Turni360 – 363Combined sources4
Helixi367 – 369Combined sources3
Beta strandi373 – 377Combined sources5
Turni384 – 386Combined sources3
Helixi387 – 394Combined sources8
Beta strandi399 – 401Combined sources3
Turni404 – 406Combined sources3
Beta strandi409 – 411Combined sources3
Beta strandi416 – 422Combined sources7
Beta strandi427 – 429Combined sources3
Beta strandi432 – 434Combined sources3
Helixi438 – 442Combined sources5
Beta strandi445 – 449Combined sources5
Helixi463 – 475Combined sources13
Beta strandi483 – 485Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1S9HX-ray2.40A/B/C225-490[»]
1U0JX-ray2.10A225-490[»]
4ZO0X-ray2.30A/B/C1-206[»]
4ZQ9X-ray2.60A/B/C1-208[»]
5DCXX-ray2.60A/B/C/D/E/F/G/H/I/J/K1-224[»]
ProteinModelPortaliP03132.
SMRiP03132.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP03132.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini308 – 463SF3 helicasePROSITE-ProRule annotationAdd BLAST156

Sequence similaritiesi

Contains 1 SF3 helicase domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR014015. Helicase_SF3_DNA-vir.
IPR027417. P-loop_NTPase.
IPR001257. Parvovirus_NS1_helicase.
IPR014835. Rep_N.
[Graphical view]
PfamiPF01057. Parvo_NS1. 1 hit.
PF08724. Rep_N. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS51206. SF3_HELICASE_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P03132-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPGFYEIVIK VPSDLDGHLP GISDSFVNWV AEKEWELPPD SDMDLNLIEQ
60 70 80 90 100
APLTVAEKLQ RDFLTEWRRV SKAPEALFFV QFEKGESYFH MHVLVETTGV
110 120 130 140 150
KSMVLGRFLS QIREKLIQRI YRGIEPTLPN WFAVTKTRNG AGGGNKVVDE
160 170 180 190 200
CYIPNYLLPK TQPELQWAWT NMEQYLSACL NLTERKRLVA QHLTHVSQTQ
210 220 230 240 250
EQNKENQNPN SDAPVIRSKT SARYMELVGW LVDKGITSEK QWIQEDQASY
260 270 280 290 300
ISFNAASNSR SQIKAALDNA GKIMSLTKTA PDYLVGQQPV EDISSNRIYK
310 320 330 340 350
ILELNGYDPQ YAASVFLGWA TKKFGKRNTI WLFGPATTGK TNIAEAIAHT
360 370 380 390 400
VPFYGCVNWT NENFPFNDCV DKMVIWWEEG KMTAKVVESA KAILGGSKVR
410 420 430 440 450
VDQKCKSSAQ IDPTPVIVTS NTNMCAVIDG NSTTFEHQQP LQDRMFKFEL
460 470 480 490 500
TRRLDHDFGK VTKQEVKDFF RWAKDHVVEV EHEFYVKKGG AKKRPAPSDA
510 520 530
DISEPKRVRE SVAQPSTSDA EASINYADRL ARGHSL
Length:536
Mass (Da):60,754
Last modified:July 21, 1986 - v1
Checksum:i7C1ECDD4E07703C8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti17G → E in AAC03774 (PubMed:7996133).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01901 Genomic DNA. Translation: AAA42372.1.
AF043303 Genomic DNA. Translation: AAC03774.1.
PIRiA03694. UYAD1A.
RefSeqiYP_680422.1. NC_001401.2.

Genome annotation databases

GeneIDi4192013.
KEGGivg:4192013.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01901 Genomic DNA. Translation: AAA42372.1.
AF043303 Genomic DNA. Translation: AAC03774.1.
PIRiA03694. UYAD1A.
RefSeqiYP_680422.1. NC_001401.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1S9HX-ray2.40A/B/C225-490[»]
1U0JX-ray2.10A225-490[»]
4ZO0X-ray2.30A/B/C1-206[»]
4ZQ9X-ray2.60A/B/C1-208[»]
5DCXX-ray2.60A/B/C/D/E/F/G/H/I/J/K1-224[»]
ProteinModelPortaliP03132.
SMRiP03132.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP03132. 4 interactors.
MINTiMINT-6732280.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi4192013.
KEGGivg:4192013.

Miscellaneous databases

EvolutionaryTraceiP03132.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR014015. Helicase_SF3_DNA-vir.
IPR027417. P-loop_NTPase.
IPR001257. Parvovirus_NS1_helicase.
IPR014835. Rep_N.
[Graphical view]
PfamiPF01057. Parvo_NS1. 1 hit.
PF08724. Rep_N. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS51206. SF3_HELICASE_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiREP68_AAV2S
AccessioniPrimary (citable) accession number: P03132
Secondary accession number(s): O56650
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: November 2, 2016
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.