Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P03129

- VE7_HPV16

UniProt

P03129 - VE7_HPV16

Protein

Protein E7

Gene

E7

Organism
Human papillomavirus type 16
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    E7 protein has both transforming and trans-activating activities. Disrupts the function of host retinoblastoma protein RB1/pRb, which is a key regulator of the cell cycle. Induces the disassembly of the E2F1 transcription factors from RB1, with subsequent transcriptional activation of E2F1-regulated S-phase genes. Inactivation of the ability of RB1 to arrest the cell cycle is critical for cellular transformation, uncontrolled cellular growth and proliferation induced by viral infection. Stimulation of progression from G1 to S phase allows the virus to efficiently use the cellular DNA replicating machinery to achieve viral genome replication. Interferes with histone deacetylation mediated by HDAC1 and HDAC2, leading to activation of transcription By similarity.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri58 – 9437Sequence AnalysisAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. protein binding Source: UniProtKB
    4. sequence-specific DNA binding transcription factor activity Source: InterPro

    GO - Biological processi

    1. modulation by virus of host G1/S transition checkpoint Source: UniProtKB-KW
    2. suppression by virus of host IRF9 activity Source: UniProtKB-KW
    3. suppression by virus of host type I interferon-mediated signaling pathway Source: UniProtKB-KW
    4. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Eukaryotic host gene expression shutoff by virus, Eukaryotic host transcription shutoff by virus, G1/S host cell cycle checkpoint dysregulation by virus, Host gene expression shutoff by virus, Host-virus interaction, Inhibition of eukaryotic host transcription initiation by virus, Inhibition of host innate immune response by virus, Inhibition of host interferon signaling pathway by virus, Inhibition of host IRF9 by virus, Modulation of host cell cycle by virus, Transcription, Transcription regulation, Viral immunoevasion

    Keywords - Ligandi

    DNA-binding, Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein E7
    Gene namesi
    Name:E7
    OrganismiHuman papillomavirus type 16
    Taxonomic identifieri333760 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePapillomaviridaeAlphapapillomavirus
    Virus hostiHomo sapiens (Human) [TaxID: 9606]
    ProteomesiUP000009251: Genome

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi67 – 671L → R: Complete loss of interaction with HDAC1 and transformation ability. 1 Publication
    Mutagenesisi82 – 832LL → RR: Complete loss of interaction with HDAC1 and transformation ability.
    Mutagenesisi91 – 911C → G: Complete loss of interaction with HDAC1 and transformation ability. 1 Publication

    Keywords - Diseasei

    Oncogene

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 9898Protein E7PRO_0000133414Add
    BLAST

    Expressioni

    Keywords - Developmental stagei

    Early protein

    Interactioni

    Subunit structurei

    Homodimer. Homooligomer. Interaction with host RB1 induces the aberrant dissociation of RB1-E2F1 complex thereby disrupting RB1's activity. Binds to CHD3 through its zinc-finger domain. Forms a complex with CHD3 and HDAC1, thereby altering the action of host histone deacetylation. A similar complex involving E7, CHD3 and HDAC2 might also form. Interacts with E2; this interaction inhibits E7 oncogenic activity.6 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    FOXM1Q08050-13EBI-866453,EBI-866499From a different organism.
    IRF1P109143EBI-866453,EBI-1055781From a different organism.
    KAT2BQ928315EBI-866453,EBI-477430From a different organism.
    MYCP011062EBI-866453,EBI-447544From a different organism.
    PPP2CAP677753EBI-866453,EBI-712311From a different organism.
    PPP2R1AP301533EBI-866453,EBI-302388From a different organism.
    RB1P0640021EBI-866453,EBI-491274From a different organism.
    SIVA1O153044EBI-866453,EBI-520756From a different organism.
    UBR4Q5T4S74EBI-866453,EBI-1995940From a different organism.
    USP11P517846EBI-866453,EBI-306876From a different organism.

    Protein-protein interaction databases

    DIPiDIP-1090N.
    IntActiP03129. 25 interactions.
    MINTiMINT-87652.

    Structurei

    3D structure databases

    DisProtiDP00024.
    ProteinModelPortaliP03129.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 4040DisorderedSequence AnalysisAdd
    BLAST
    Regioni22 – 265Interaction with RB1Sequence Analysis

    Sequence similaritiesi

    Belongs to the papillomaviridae E7 protein family.Curated

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri58 – 9437Sequence AnalysisAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Family and domain databases

    InterProiIPR000148. Papilloma_E7.
    [Graphical view]
    PfamiPF00527. E7. 1 hit.
    [Graphical view]
    PIRSFiPIRSF003407. Papvi_E7. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P03129-1 [UniParc]FASTAAdd to Basket

    « Hide

    MHGDTPTLHE YMLDLQPETT DLYCYEQLND SSEEEDEIDG PAGQAEPDRA   50
    HYNIVTFCCK CDSTLRLCVQ STHVDIRTLE DLLMGTLGIV CPICSQKP 98
    Length:98
    Mass (Da):11,022
    Last modified:July 21, 1986 - v1
    Checksum:i9BD612534CD2C9EB
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    K02718 Genomic DNA. Translation: AAA46940.1.
    D00735 Genomic DNA. Translation: BAA00633.1.
    AF003020 Genomic DNA. Translation: AAB70737.1.
    AF003023 Genomic DNA. Translation: AAB70740.1.
    AF003024 Genomic DNA. Translation: AAB70741.1.
    AF003025 Genomic DNA. Translation: AAB70742.1.
    AF003026 Genomic DNA. Translation: AAB70743.1.
    U76411 Genomic DNA. Translation: AAB18962.1.
    U76412 Genomic DNA. Translation: AAB18963.1.
    U76413 Genomic DNA. Translation: AAB18964.1.
    AF536179 Genomic DNA. Translation: AAQ10713.1.
    AF536180 Genomic DNA. Translation: AAQ10721.1.
    PIRiA03688. W7WLHS.
    RefSeqiNP_041326.1. NC_001526.2.

    Genome annotation databases

    GeneIDi1489079.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    K02718 Genomic DNA. Translation: AAA46940.1 .
    D00735 Genomic DNA. Translation: BAA00633.1 .
    AF003020 Genomic DNA. Translation: AAB70737.1 .
    AF003023 Genomic DNA. Translation: AAB70740.1 .
    AF003024 Genomic DNA. Translation: AAB70741.1 .
    AF003025 Genomic DNA. Translation: AAB70742.1 .
    AF003026 Genomic DNA. Translation: AAB70743.1 .
    U76411 Genomic DNA. Translation: AAB18962.1 .
    U76412 Genomic DNA. Translation: AAB18963.1 .
    U76413 Genomic DNA. Translation: AAB18964.1 .
    AF536179 Genomic DNA. Translation: AAQ10713.1 .
    AF536180 Genomic DNA. Translation: AAQ10721.1 .
    PIRi A03688. W7WLHS.
    RefSeqi NP_041326.1. NC_001526.2.

    3D structure databases

    DisProti DP00024.
    ProteinModelPortali P03129.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-1090N.
    IntActi P03129. 25 interactions.
    MINTi MINT-87652.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 1489079.

    Family and domain databases

    InterProi IPR000148. Papilloma_E7.
    [Graphical view ]
    Pfami PF00527. E7. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF003407. Papvi_E7. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Expression of the human papillomavirus type 16 genome in SK-v cells, a line derived from a vulvar intraepithelial neoplasia."
      Schneider-Maunoury S., Pehau-Arnaudet G., Breitburd F., Orth G.
      J. Gen. Virol. 71:809-817(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Sequence variations and viral genomic state of human papillomavirus type 16 in penile carcinomas from Ugandan patients."
      Tornesello M.L., Buonaguro F.M., Meglio A., Buonaguro L., Beth-Giraldo E., Giraldo G.
      J. Gen. Virol. 78:2199-2208(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    4. "Major sequence variations in E7 gene of human papillomavirus type 16 from cervical cancerous and non-cancerous lesions of Korean women."
      Song Y.S., Kee S.H., Kim J.W., Park N.H., Kang S.B., Lee H.P.
      Submitted (NOV-1996) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    5. "Cloning and sequencing of non-European human papillomavirus (HPV) variant complete genomes from cervicovaginal cells by an overlapping PCR method."
      Terai M., Fu L., Ma Z., Burk R.D.
      Submitted (AUG-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Isolate African 1 and Isolate European German 131.
    6. "The human papillomavirus type 16 E7 gene encodes transactivation and transformation functions similar to those of adenovirus E1A."
      Phelps W.C., Yee C.L., Munger K., Howley P.M.
      Cell 53:539-547(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "Adenovirus E1A, simian virus 40 tumor antigen, and human papillomavirus E7 protein share the capacity to disrupt the interaction between transcription factor E2F and the retinoblastoma gene product."
      Chellappan S., Kraus V.B., Kroger B., Munger K., Howley P.M., Phelps W.C., Nevins J.R.
      Proc. Natl. Acad. Sci. U.S.A. 89:4549-4553(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH HUMAN RB1.
    8. "The E7 oncoprotein associates with Mi2 and histone deacetylase activity to promote cell growth."
      Brehm A., Nielsen S.J., Miska E.A., McCance D.J., Reid J.L., Bannister A.J., Kouzarides T.
      EMBO J. 18:2449-2458(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CHD3, IDENTIFICATION IN A COMPLEX WITH HUMAN HDAC1 AND CHD3, MUTAGENESIS OF LEU-67; 82-LEU-LEU-83 AND CYS-91.
    9. "Translational regulation of human papillomavirus type 16 E7 mRNA by the peptide SEQIKA, shared by rabbit alpha(1)-globin and human cytokeratin 7."
      Kanduc D.
      J. Virol. 76:7040-7048(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH HUMAN KRT7.
    10. "The HPV16 E7 viral oncoprotein self-assembles into defined spherical oligomers."
      Alonso L.G., Garcia-Alai M.M., Smal C., Centeno J.M., Iacono R., Castano E., Gualfetti P., de Prat-Gay G.
      Biochemistry 43:3310-3317(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBUNIT.
    11. "Association of the human papillomavirus type 16 E7 oncoprotein with the 600-kDa retinoblastoma protein-associated factor, p600."
      Huh K.-W., DeMasi J., Ogawa H., Nakatani Y., Howley P.M., Muenger K.
      Proc. Natl. Acad. Sci. U.S.A. 102:11492-11497(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH HUMAN ZUBR1.
    12. "Regulation of human papillomavirus type 16 E7 activity through direct protein interaction with the E2 transcriptional activator."
      Gammoh N., Grm H.S., Massimi P., Banks L.
      J. Virol. 80:1787-1797(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH E2.
    13. "The N-terminal module of HPV16 E7 is an intrinsically disordered domain that confers conformational and recognition plasticity to the oncoprotein."
      Garcia-Alai M.M., Alonso L.G., de Prat-Gay G.
      Biochemistry 46:10405-10412(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISORDERED REGION.
    14. "Interactions of SV40 large T antigen and other viral proteins with retinoblastoma tumour suppressor."
      Lee C., Cho Y.
      Rev. Med. Virol. 12:81-92(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW.

    Entry informationi

    Entry nameiVE7_HPV16
    AccessioniPrimary (citable) accession number: P03129
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: July 21, 1986
    Last modified: October 1, 2014
    This is version 96 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    HPV16, in comparison to HPV types 6 and 11, is more often associated with malignant genital cancers in humans.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3