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P03129 (VE7_HPV16) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein E7
Gene names
Name:E7
OrganismHuman papillomavirus type 16 [Reference proteome]
Taxonomic identifier333760 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stagePapillomaviridaeAlphapapillomavirus
Virus hostHomo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length98 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

E7 protein has both transforming and trans-activating activities. Disrupts the function of host retinoblastoma protein RB1/pRb, which is a key regulator of the cell cycle. Induces the disassembly of the E2F1 transcription factors from RB1, with subsequent transcriptional activation of E2F1-regulated S-phase genes. Inactivation of the ability of RB1 to arrest the cell cycle is critical for cellular transformation, uncontrolled cellular growth and proliferation induced by viral infection. Stimulation of progression from G1 to S phase allows the virus to efficiently use the cellular DNA replicating machinery to achieve viral genome replication. Interferes with histone deacetylation mediated by HDAC1 and HDAC2, leading to activation of transcription By similarity. Ref.6

Subunit structure

Homodimer. Homooligomer. Interaction with host RB1 induces the aberrant dissociation of RB1-E2F1 complex thereby disrupting RB1's activity. Binds to CHD3 through its zinc-finger domain. Forms a complex with CHD3 and HDAC1, thereby altering the action of host histone deacetylation. A similar complex involving E7, CHD3 and HDAC2 might also form. Interacts with E2; this interaction inhibits E7 oncogenic activity. Ref.7 Ref.8 Ref.9 Ref.10 Ref.11 Ref.12

Miscellaneous

HPV16, in comparison to HPV types 6 and 11, is more often associated with malignant genital cancers in humans.

Sequence similarities

Belongs to the papillomaviridae E7 protein family.

Ontologies

Keywords
   Biological processEukaryotic host gene expression shutoff by virus
Eukaryotic host transcription shutoff by virus
G1/S host cell cycle checkpoint dysregulation by virus
Host gene expression shutoff by virus
Host-virus interaction
Inhibition of eukaryotic host transcription initiation by virus
Inhibition of host innate immune response by virus
Inhibition of host interferon signaling pathway by virus
Inhibition of host IRF9 by virus
Modulation of host cell cycle by virus
Transcription
Transcription regulation
Viral immunoevasion
   Developmental stageEarly protein
   DiseaseOncogene
   DomainZinc-finger
   LigandDNA-binding
Metal-binding
Zinc
   Molecular functionActivator
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processmodulation by virus of host G1/S transition checkpoint

Inferred from electronic annotation. Source: UniProtKB-KW

suppression by virus of host IRF9 activity

Inferred from electronic annotation. Source: UniProtKB-KW

suppression by virus of host transcription initiation from RNA polymerase II promoter

Inferred from electronic annotation. Source: UniProtKB-KW

suppression by virus of host type I interferon-mediated signaling pathway

Inferred from electronic annotation. Source: UniProtKB-KW

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

protein binding

Inferred from physical interaction PubMed 10523841Ref.9. Source: UniProtKB

sequence-specific DNA binding transcription factor activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

FOXM1Q08050-13EBI-866453,EBI-866499From a different organism.
IRF1P109143EBI-866453,EBI-1055781From a different organism.
KAT2BQ928315EBI-866453,EBI-477430From a different organism.
MYCP011062EBI-866453,EBI-447544From a different organism.
PPP2CAP677753EBI-866453,EBI-712311From a different organism.
PPP2R1AP301533EBI-866453,EBI-302388From a different organism.
RB1P0640021EBI-866453,EBI-491274From a different organism.
SIVA1O153044EBI-866453,EBI-520756From a different organism.
UBR4Q5T4S74EBI-866453,EBI-1995940From a different organism.
USP11P517846EBI-866453,EBI-306876From a different organism.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 9898Protein E7
PRO_0000133414

Regions

Zinc finger58 – 9437 Potential
Region1 – 4040Disordered Potential
Region22 – 265Interaction with RB1 Potential

Experimental info

Mutagenesis671L → R: Complete loss of interaction with HDAC1 and transformation ability. Ref.8
Mutagenesis82 – 832LL → RR: Complete loss of interaction with HDAC1 and transformation ability.
Mutagenesis911C → G: Complete loss of interaction with HDAC1 and transformation ability. Ref.8

Sequences

Sequence LengthMass (Da)Tools
P03129 [UniParc].

Last modified July 21, 1986. Version 1.
Checksum: 9BD612534CD2C9EB

FASTA9811,022
        10         20         30         40         50         60 
MHGDTPTLHE YMLDLQPETT DLYCYEQLND SSEEEDEIDG PAGQAEPDRA HYNIVTFCCK 

        70         80         90 
CDSTLRLCVQ STHVDIRTLE DLLMGTLGIV CPICSQKP 

« Hide

References

[1]"Human papillomavirus type 16 DNA sequence."
Seedorf K., Krammer G., Durst M., Suhai S., Rowekamp W.G.
Virology 145:181-185(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Expression of the human papillomavirus type 16 genome in SK-v cells, a line derived from a vulvar intraepithelial neoplasia."
Schneider-Maunoury S., Pehau-Arnaudet G., Breitburd F., Orth G.
J. Gen. Virol. 71:809-817(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Sequence variations and viral genomic state of human papillomavirus type 16 in penile carcinomas from Ugandan patients."
Tornesello M.L., Buonaguro F.M., Meglio A., Buonaguro L., Beth-Giraldo E., Giraldo G.
J. Gen. Virol. 78:2199-2208(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"Major sequence variations in E7 gene of human papillomavirus type 16 from cervical cancerous and non-cancerous lesions of Korean women."
Song Y.S., Kee S.H., Kim J.W., Park N.H., Kang S.B., Lee H.P.
Submitted (NOV-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]"Cloning and sequencing of non-European human papillomavirus (HPV) variant complete genomes from cervicovaginal cells by an overlapping PCR method."
Terai M., Fu L., Ma Z., Burk R.D.
Submitted (AUG-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Isolate African 1 and Isolate European German 131.
[6]"The human papillomavirus type 16 E7 gene encodes transactivation and transformation functions similar to those of adenovirus E1A."
Phelps W.C., Yee C.L., Munger K., Howley P.M.
Cell 53:539-547(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[7]"Adenovirus E1A, simian virus 40 tumor antigen, and human papillomavirus E7 protein share the capacity to disrupt the interaction between transcription factor E2F and the retinoblastoma gene product."
Chellappan S., Kraus V.B., Kroger B., Munger K., Howley P.M., Phelps W.C., Nevins J.R.
Proc. Natl. Acad. Sci. U.S.A. 89:4549-4553(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH HUMAN RB1.
[8]"The E7 oncoprotein associates with Mi2 and histone deacetylase activity to promote cell growth."
Brehm A., Nielsen S.J., Miska E.A., McCance D.J., Reid J.L., Bannister A.J., Kouzarides T.
EMBO J. 18:2449-2458(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH CHD3, IDENTIFICATION IN A COMPLEX WITH HUMAN HDAC1 AND CHD3, MUTAGENESIS OF LEU-67; 82-LEU-LEU-83 AND CYS-91.
[9]"Translational regulation of human papillomavirus type 16 E7 mRNA by the peptide SEQIKA, shared by rabbit alpha(1)-globin and human cytokeratin 7."
Kanduc D.
J. Virol. 76:7040-7048(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH HUMAN KRT7.
[10]"The HPV16 E7 viral oncoprotein self-assembles into defined spherical oligomers."
Alonso L.G., Garcia-Alai M.M., Smal C., Centeno J.M., Iacono R., Castano E., Gualfetti P., de Prat-Gay G.
Biochemistry 43:3310-3317(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBUNIT.
[11]"Association of the human papillomavirus type 16 E7 oncoprotein with the 600-kDa retinoblastoma protein-associated factor, p600."
Huh K.-W., DeMasi J., Ogawa H., Nakatani Y., Howley P.M., Muenger K.
Proc. Natl. Acad. Sci. U.S.A. 102:11492-11497(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH HUMAN ZUBR1.
[12]"Regulation of human papillomavirus type 16 E7 activity through direct protein interaction with the E2 transcriptional activator."
Gammoh N., Grm H.S., Massimi P., Banks L.
J. Virol. 80:1787-1797(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH E2.
[13]"The N-terminal module of HPV16 E7 is an intrinsically disordered domain that confers conformational and recognition plasticity to the oncoprotein."
Garcia-Alai M.M., Alonso L.G., de Prat-Gay G.
Biochemistry 46:10405-10412(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: DISORDERED REGION.
[14]"Interactions of SV40 large T antigen and other viral proteins with retinoblastoma tumour suppressor."
Lee C., Cho Y.
Rev. Med. Virol. 12:81-92(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
K02718 Genomic DNA. Translation: AAA46940.1.
D00735 Genomic DNA. Translation: BAA00633.1.
AF003020 Genomic DNA. Translation: AAB70737.1.
AF003023 Genomic DNA. Translation: AAB70740.1.
AF003024 Genomic DNA. Translation: AAB70741.1.
AF003025 Genomic DNA. Translation: AAB70742.1.
AF003026 Genomic DNA. Translation: AAB70743.1.
U76411 Genomic DNA. Translation: AAB18962.1.
U76412 Genomic DNA. Translation: AAB18963.1.
U76413 Genomic DNA. Translation: AAB18964.1.
AF536179 Genomic DNA. Translation: AAQ10713.1.
AF536180 Genomic DNA. Translation: AAQ10721.1.
PIRW7WLHS. A03688.
RefSeqNP_041326.1. NC_001526.2.

3D structure databases

DisProtDP00024.
ProteinModelPortalP03129.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-1090N.
IntActP03129. 25 interactions.
MINTMINT-87652.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1489079.

Family and domain databases

InterProIPR000148. Papilloma_E7.
[Graphical view]
PfamPF00527. E7. 1 hit.
[Graphical view]
PIRSFPIRSF003407. Papvi_E7. 1 hit.
ProtoNetSearch...

Entry information

Entry nameVE7_HPV16
AccessionPrimary (citable) accession number: P03129
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: July 9, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families