Reviewed, UniProtKB/Swiss-Prot P03126 (VE6_HPV16)

Names and origin

Protein names

Recommended name:
    Protein E6

Gene names

Name:

E6

Organism

Human papillomavirus type 16

Taxonomic identifier

333760 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Papillomaviridae › Alphapapillomavirus

Virus host

Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	158 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Transcriptional transactivator. Binds double stranded DNA. Has transforming activity. Inactivates, with E6-AP ubiquitin-protein ligase, the human TP53/p53 tumor suppressor protein by targeting it to degradation. Binds and targets human MUPP1/MPDZ protein to degradation. Those two functions presumably contribute to transforming activity By similarity. Interaction with human FBLN1 protein also seems to be linked to cell transformation.
Subunit structure	Forms a complex with E6-AP ubiquitin-protein ligase which interacts with human P53. Binds to human FBLN1 and MPDZ By similarity. Interacts with human NFX1 and MAGI3. Ref.4 Ref.5 Ref.6 Ref.7 Ref.8
Subcellular location	Host nucleus matrix.
Miscellaneous	HPV16, in comparison to HPV types 6 and 11, is more often associated with malignant genital cancers in humans.
Sequence similarities	Belongs to the papillomaviridae E6 protein family.

Ontologies

Keywords
Biological process	Host-virus interaction Transcription Transcription regulation
Cellular component	Host nucleus
Developmental stage	Early protein
Disease	Oncogene
Domain	Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Activator
Technical term	3D-structure
Gene Ontology (GO)
Biological process	interspecies interaction between organisms Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	host cell nuclear matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
TP73	O15350-1	1	EBI-1177242,EBI-389619	From a different organism.
TP73	O15350-2	1	EBI-1177242,EBI-389623	From a different organism.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 158

158

Protein E6

PRO_0000133336

Regions

Zinc finger

37 – 73

37

Potential

Zinc finger

110 – 146

37

Potential

Motif

155 – 158

4

PDZ-binding By similarity

Experimental info

Sequence conflict

17

1

R → G in AAQ10712. Ref.2

Sequence conflict

90

1

L → V in AAQ10712. Ref.2

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

158

Helix Strand Turn

Details...

Beta strand

87 – 90

4

Helix

93 – 100

8

Turn

101 – 105

5

Beta strand

111 – 113

3

Helix

119 – 128

10

Beta strand

132 – 135

4

Beta strand

138 – 141

4

Helix

142 – 146

5

Sequences

	Sequence		Length	Mass (Da)
	P03126-1 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: 01FEF5ADCFDB37EB	FASTA	158	19,187
	10 20 30 40 50 60 MHQKRTAMFQ DPQERPRKLP QLCTELQTTI HDIILECVYC KQQLLRREVY DFAFRDLCIV 70 80 90 100 110 120 YRDGNPYAVC DKCLKFYSKI SEYRHYCYSL YGTTLEQQYN KPLCDLLIRC INCQKPLCPE 130 140 150 EKQRHLDKKQ RFHNIRGRWT GRCMSCCRSS RTRRETQL

References

[1]	"Human papillomavirus type 16 DNA sequence." Seedorf K., Krammer G., Durst M., Suhai S., Rowekamp W.G. Virology 145:181-185(1985) [PubMed: 2990099] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Cloning and sequencing of non-European human papillomavirus (HPV) variant complete genomes from cervicovaginal cells by an overlapping PCR method." Terai M., Fu L., Ma Z., Burk R.D. Submitted (AUG-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Isolate European German 131.
[3]	"Expression of the human papillomavirus type 16 genome in SK-v cells, a line derived from a vulvar intraepithelial neoplasia." Schneider-Maunoury S., Pehau-Arnaudet G., Breitburd F., Orth G. J. Gen. Virol. 71:809-817(1990) [PubMed: 2157796] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 31-50.
[4]	"Interaction of oncogenic papillomavirus E6 proteins with fibulin-1." Du M., Fan X., Hong E., Chen J.J. Biochem. Biophys. Res. Commun. 296:962-969(2002) [PubMed: 12200142] [Abstract] Cited for: INTERACTION WITH HUMAN FBLN1, INHIBITION OF E6-MEDIATED TRANSFORMATION.
[5]	"Identification of a novel telomerase repressor that interacts with the human papillomavirus type-16 E6/E6-AP complex." Gewin L., Myers H., Kiyono T., Galloway D.A. Genes Dev. 18:2269-2282(2004) [PubMed: 15371341] [Abstract] Cited for: INTERACTION WITH HUMAN NFX1.
[6]	"Oncogenic human papillomavirus E6 proteins target the MAGI-2 and MAGI-3 proteins for degradation." Thomas M., Laura R., Hepner K., Guccione E., Sawyers C., Lasky L., Banks L. Oncogene 21:5088-5096(2002) [PubMed: 12140759] [Abstract] Cited for: INTERACTION WITH HUMAN MAGI3.
[7]	"Role of the PDZ domain-binding motif of the oncoprotein E6 in the pathogenesis of human papillomavirus type 31." Lee C., Laimins L.A. J. Virol. 78:12366-12377(2004) [PubMed: 15507623] [Abstract] Cited for: INTERACTION WITH HUMAN MAGI3.
[8]	"HPV E6 specifically targets different cellular pools of its PDZ domain-containing tumour suppressor substrates for proteasome-mediated degradation." Massimi P., Gammoh N., Thomas M., Banks L. Oncogene 23:8033-8039(2004) [PubMed: 15378012] [Abstract] Cited for: INTERACTION WITH HUMAN MAGI3.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

K02718 Genomic DNA. Translation: AAA46939.1.
AF536179 Genomic DNA. Translation: AAQ10712.1.
D00735 Genomic DNA. Translation: BAA00632.1.

PIR

W6WLHS. A03682.

RefSeq

NP_041325.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1VZN	model	-	A	1-158	[»]
2FK4	NMR	-	A	88-158	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

P03126. 5 interactions.

Genome annotation databases

GeneID

1489078.

Family and domain databases

InterPro

IPR001334. E6.
[Graphical view]

Pfam

PF00518. E6. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

VE6_HPV16

Accession

Primary (citable) accession number: P03126
Secondary accession number(s): Q71BI7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	January 19, 2010
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)