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P03116 (VE1_BPV1) Reviewed, UniProtKB/Swiss-Prot

Last modified January 11, 2011. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Replication protein E1
EC=3.6.4.12
Alternative name(s):
ATP-dependent helicase E1
Gene names
Name:E1
OrganismBovine papillomavirus type 1 [Complete proteome]
Taxonomic identifier10559 [NCBI]
Taxonomic lineageViruses › dsDNA viruses, no RNA stage › Papillomaviridae › Deltapapillomavirus
Virus hostBos taurus (Bovine) [TaxID: 9913]

Protein attributes

Sequence length605 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

ATP-dependent DNA helicase required for initiation of viral DNA replication. It forms a complex with the viral E2 protein. The E1-E2 complex binds to the replication origin which contains binding sites for both proteins.

Catalytic activity

ATP + H2O = ADP + phosphate.

Subcellular location

Host nucleus.

Sequence similarities

Belongs to the papillomaviridae E1 protein family.

Contains 1 SF3 helicase domain.

Ontologies

Keywords
   Biological processDNA replication
   Cellular componentHost nucleus
   Developmental stageEarly protein
   LigandATP-binding
DNA-binding
Nucleotide-binding
   Molecular functionHelicase
Hydrolase
   Technical term3D-structure
Complete proteome
Gene Ontology (GO)
   Biological processDNA replication

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componenthost cell nucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

ATP-dependent DNA helicase activity

Inferred from electronic annotation. Source: InterPro

DNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 605605Replication protein E1
PRO_0000133090

Regions

Domain407 – 557151SF3 helicase
Nucleotide binding433 – 4408ATP Potential

Secondary structure

1 ......................................................................... 605
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P03116 [UniParc].

Last modified July 21, 1986. Version 1.
Checksum: C8400B7B8F606E0B

FASTA60568,190
        10         20         30         40         50         60 
MANDKGSNWD SGLGCSYLLT EAECESDKEN EEPGAGVELS VESDRYDSQD EDFVDNASVF 

        70         80         90        100        110        120 
QGNHLEVFQA LEKKAGEEQI LNLKRKVLGS SQNSSGSEAS ETPVKRRKSG AKRRLFAENE 

       130        140        150        160        170        180 
ANRVLTPLQV QGEGEGRQEL NEEQAISHLH LQLVKSKNAT VFKLGLFKSL FLCSFHDITR 

       190        200        210        220        230        240 
LFKNDKTTNQ QWVLAVFGLA EVFFEASFEL LKKQCSFLQM QKRSHEGGTC AVYLICFNTA 

       250        260        270        280        290        300 
KSRETVRNLM ANTLNVREEC LMLQPAKIRG LSAALFWFKS SLSPATLKHG ALPEWIRAQT 

       310        320        330        340        350        360 
TLNESLQTEK FDFGTMVQWA YDHKYAEESK IAYEYALAAG SDSNARAFLA TNSQAKHVKD 

       370        380        390        400        410        420 
CATMVRHYLR AETQALSMPA YIKARCKLAT GEGSWKSILT FFNYQNIELI TFINALKLWL 

       430        440        450        460        470        480 
KGIPKKNCLA FIGPPNTGKS MLCNSLIHFL GGSVLSFANH KSHFWLASLA DTRAALVDDA 

       490        500        510        520        530        540 
THACWRYFDT YLRNALDGYP VSIDRKHKAA VQIKAPPLLV TSNIDVQAED RYLYLHSRVQ 

       550        560        570        580        590        600 
TFRFEQPCTD ESGEQPFNIT DADWKSFFVR LWGRLDLIDE EEDSEEDGDS MRTFTCSARN 


TNAVD

« Hide

References

[1]"The primary structure and genetic organization of the bovine papillomavirus type 1 genome."
Chen E.Y., Howley P.M., Levinson A.D., Seeburg P.H.
Nature 299:529-534(1982) [PubMed: 6289124] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Transient replication of BPV-1 requires two viral polypeptides encoded by the E1 and E2 open reading frames."
Ustav M., Stenlung A.
EMBO J. 10:449-457(1991) [PubMed: 1846806] [Abstract]
Cited for: REQUIREMENT FOR REPLICATION.
[3]"The E1 protein of bovine papilloma virus 1 is an ATP-dependent DNA helicase."
Yang L., Mohr I., Fouts E., Lim D.A., Nohaile M., Botchan M.
Proc. Natl. Acad. Sci. U.S.A. 90:5086-5090(1993) [PubMed: 8389467] [Abstract]
Cited for: CHARACTERIZATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X02346 Genomic DNA. Translation: CAB46511.1.
PIRW1WLEB. A03663.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1F08X-ray1.90A/B159-303[»]
1KSXX-ray3.20A/B/E/F/I/J/M/N159-303[»]
1KSYX-ray3.05A/B/C159-309[»]
2GXAX-ray3.15A/B/C/D/E/F/G/H/I/J/K/L305-577[»]
2V9PX-ray3.00A/B/C/D/E/F/G/H/I/J/K/L301-605[»]
ProteinModelPortalP03116.
SMRP03116. Positions 159-303, 305-577.
ModBaseSearch...

Protein-protein interaction databases

MINTMINT-91530.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

ProtClustDBPHA2774.

Family and domain databases

InterProIPR001177. DNA_helicase_E1_C_Papillomavir.
IPR014000. DNA_helicase_E1_N_Papillomavir.
IPR014015. Helicase_SF3_DNA-vir.
IPR016393. Rep_E1_papillomaV.
[Graphical view]
PfamPF00519. PPV_E1_C. 1 hit.
PF00524. PPV_E1_N. 1 hit.
[Graphical view]
PIRSFPIRSF003383. Rep_E1_papillomaV. 1 hit.
PROSITEPS51206. SF3_HELICASE_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameVE1_BPV1
AccessionPrimary (citable) accession number: P03116
Secondary accession number(s): Q9WMH1
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: January 11, 2011
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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