Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Major capsid protein L1

Gene

L1

Organism
Human papillomavirus type 16
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Forms an icosahedral capsid with a T=7 symmetry and a 50 nm diameter. The capsid is composed of 72 pentamers linked to each other by disulfide bonds and associated with L2 proteins. Binds to heparan sulfate proteoglycans on cell surface of basal layer keratinocytes to provide initial virion attachment. This binding mediates a conformational change in the virus capsid that facilitates efficient infection. The virion enters the host cell via endocytosis. During virus trafficking, L1 protein dissociates from the viral DNA and the genomic DNA is released to the host nucleus. The virion assembly takes place within the cell nucleus. Encapsulates the genomic DNA together with protein L2.UniRule annotation3 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Host-virus interaction, Viral attachment to host cell, Viral penetration into host cytoplasm, Virus endocytosis by host, Virus entry into host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Major capsid protein L1UniRule annotation
Gene namesi
Name:L1UniRule annotation
OrganismiHuman papillomavirus type 16
Taxonomic identifieri333760 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePapillomaviridaeAlphapapillomavirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000009251 Componenti: Genome

Subcellular locationi

  • Virion UniRule annotation
  • Host nucleus UniRule annotation

GO - Cellular componenti

  • host cell nucleus Source: UniProtKB-SubCell
  • T=7 icosahedral viral capsid Source: CACAO
Complete GO annotation...

Keywords - Cellular componenti

Capsid protein, Host nucleus, T=7 icosahedral capsid protein, Virion

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3562172.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001335001 – 505Major capsid protein L1Add BLAST505

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi175Interchain (with C-428)UniRule annotation1 Publication
Disulfide bondi428Interchain (with C-175)UniRule annotation1 Publication

Post-translational modificationi

ISGylated by host HERC5, this results in dominant-negative effect on virus infectivity.1 Publication

Keywords - PTMi

Disulfide bond, Ubl conjugation

Expressioni

Keywords - Developmental stagei

Late protein

Interactioni

Subunit structurei

Self-assembles into homopentamers. The capsid has an icosahedral symmetry and consists of 72 capsomers, with each capsomer being a pentamer of L1. Interacts with the minor capsid protein L2; this interaction is necessary for viral genome encapsidation. Interacts with protein E2; this interaction enhances E2-dependent replication and transcription activation (PubMed:25911730). Interacts with host KPNA2; this interaction mediates the nuclear localization of L1 capsomers (PubMed:11971900). Interacts with host ITGA6 (PubMed:11341777). Interacts with host SDC1; this interaction promotes efficient infection of keratinocytes (PubMed:26289843).UniRule annotation4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
KPNA2P522923EBI-7362698,EBI-349938From a different organism.
KPNB1Q149742EBI-7362698,EBI-286758From a different organism.

Protein-protein interaction databases

IntActiP03101. 3 interactors.
MINTiMINT-255544.

Structurei

Secondary structure

1505
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi24 – 26Combined sources3
Beta strandi29 – 38Combined sources10
Beta strandi42 – 50Combined sources9
Beta strandi55 – 57Combined sources3
Beta strandi60 – 62Combined sources3
Beta strandi71 – 76Combined sources6
Turni80 – 82Combined sources3
Turni93 – 95Combined sources3
Beta strandi96 – 109Combined sources14
Beta strandi118 – 123Combined sources6
Beta strandi125 – 128Combined sources4
Beta strandi145 – 150Combined sources6
Beta strandi153 – 162Combined sources10
Beta strandi165 – 171Combined sources7
Beta strandi176 – 178Combined sources3
Beta strandi188 – 194Combined sources7
Helixi210 – 213Combined sources4
Helixi222 – 225Combined sources4
Beta strandi229 – 232Combined sources4
Helixi234 – 239Combined sources6
Beta strandi247 – 254Combined sources8
Beta strandi256 – 263Combined sources8
Helixi273 – 275Combined sources3
Turni281 – 284Combined sources4
Beta strandi291 – 296Combined sources6
Turni302 – 304Combined sources3
Beta strandi307 – 312Combined sources6
Beta strandi317 – 320Combined sources4
Beta strandi322 – 324Combined sources3
Helixi325 – 327Combined sources3
Beta strandi330 – 335Combined sources6
Beta strandi342 – 348Combined sources7
Helixi357 – 359Combined sources3
Beta strandi360 – 382Combined sources23
Helixi385 – 394Combined sources10
Helixi397 – 401Combined sources5
Helixi414 – 425Combined sources12
Turni440 – 443Combined sources4
Beta strandi447 – 450Combined sources4
Helixi459 – 461Combined sources3
Helixi463 – 472Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DZLX-ray3.50A1-505[»]
3J6Relectron microscopy9.10A/B/C/D/E/F9-486[»]
3J7Gelectron microscopy13.60A/B/C/D/E21-474[»]
3J8Velectron microscopy13.90A/B/C/D/E21-474[»]
3J8Welectron microscopy13.00A/B/C/D/E21-474[»]
3J8Zelectron microscopy14.00A/B/C/D/E21-474[»]
3JBAelectron microscopy12.00A/B/C/D/E/F9-486[»]
ProteinModelPortaliP03101.
SMRiP03101.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP03101.

Family & Domainsi

Sequence similaritiesi

Belongs to the papillomaviridae L1 protein family.UniRule annotation

Phylogenomic databases

KOiK19260.

Family and domain databases

Gene3Di2.60.175.20. 1 hit.
HAMAPiMF_04002. PPV_L1. 1 hit.
InterProiIPR002210. Capsid_L1_Papillomavir.
IPR011222. dsDNA_vir_gr_I_capsid.
[Graphical view]
PfamiPF00500. Late_protein_L1. 1 hit.
[Graphical view]
PRINTSiPR00865. HPVCAPSIDL1.
SUPFAMiSSF88648. SSF88648. 1 hit.

Sequencei

Sequence statusi: Complete.

P03101-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLWLPSEAT VYLPPVPVSK VVSTDEYVAR TNIYYHAGTS RLLAVGHPYF
60 70 80 90 100
PIKKPNNNKI LVPKVSGLQY RVFRIHLPDP NKFGFPDTSF YNPDTQRLVW
110 120 130 140 150
ACVGVEVGRG QPLGVGISGH PLLNKLDDTE NASAYAANAG VDNRECISMD
160 170 180 190 200
YKQTQLCLIG CKPPIGEHWG KGSPCTNVAV NPGDCPPLEL INTVIQDGDM
210 220 230 240 250
VDTGFGAMDF TTLQANKSEV PLDICTSICK YPDYIKMVSE PYGDSLFFYL
260 270 280 290 300
RREQMFVRHL FNRAGAVGEN VPDDLYIKGS GSTANLASSN YFPTPSGSMV
310 320 330 340 350
TSDAQIFNKP YWLQRAQGHN NGICWGNQLF VTVVDTTRST NMSLCAAIST
360 370 380 390 400
SETTYKNTNF KEYLRHGEEY DLQFIFQLCK ITLTADVMTY IHSMNSTILE
410 420 430 440 450
DWNFGLQPPP GGTLEDTYRF VTSQAIACQK HTPPAPKEDP LKKYTFWEVN
460 470 480 490 500
LKEKFSADLD QFPLGRKFLL QAGLKAKPKF TLGKRKATPT TSSTSTTAKR

KKRKL
Length:505
Mass (Da):56,278
Last modified:January 20, 2016 - v3
Checksum:iD426CEC3DCE6B0E2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti202D → H in AAA46943 (PubMed:2990099).Curated1
Sequence conflicti266A → T in AAA46943 (PubMed:2990099).Curated1
Sequence conflicti423Missing in AAA46943 (PubMed:2990099).Curated1
Sequence conflicti439D → DD in AAA46943 (PubMed:2990099).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K02718 Genomic DNA. Translation: AAA46943.1.
AF001600 Genomic DNA. Translation: AAC31789.1.
AF125673 Genomic DNA. Translation: AAD33259.1.
AF536179 Genomic DNA. Translation: AAQ10719.1.
M96285 Genomic DNA. Translation: AAA47024.1.
A06331 Unassigned DNA. Translation: CAA00546.1.
PIRiA03640. P1WLHS.

Genome annotation databases

KEGGivg:1489082.

Cross-referencesi

Web resourcesi

Virus Particle ExploreR db

Icosahedral capsid structure

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K02718 Genomic DNA. Translation: AAA46943.1.
AF001600 Genomic DNA. Translation: AAC31789.1.
AF125673 Genomic DNA. Translation: AAD33259.1.
AF536179 Genomic DNA. Translation: AAQ10719.1.
M96285 Genomic DNA. Translation: AAA47024.1.
A06331 Unassigned DNA. Translation: CAA00546.1.
PIRiA03640. P1WLHS.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DZLX-ray3.50A1-505[»]
3J6Relectron microscopy9.10A/B/C/D/E/F9-486[»]
3J7Gelectron microscopy13.60A/B/C/D/E21-474[»]
3J8Velectron microscopy13.90A/B/C/D/E21-474[»]
3J8Welectron microscopy13.00A/B/C/D/E21-474[»]
3J8Zelectron microscopy14.00A/B/C/D/E21-474[»]
3JBAelectron microscopy12.00A/B/C/D/E/F9-486[»]
ProteinModelPortaliP03101.
SMRiP03101.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP03101. 3 interactors.
MINTiMINT-255544.

Chemistry databases

ChEMBLiCHEMBL3562172.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGivg:1489082.

Phylogenomic databases

KOiK19260.

Miscellaneous databases

EvolutionaryTraceiP03101.

Family and domain databases

Gene3Di2.60.175.20. 1 hit.
HAMAPiMF_04002. PPV_L1. 1 hit.
InterProiIPR002210. Capsid_L1_Papillomavir.
IPR011222. dsDNA_vir_gr_I_capsid.
[Graphical view]
PfamiPF00500. Late_protein_L1. 1 hit.
[Graphical view]
PRINTSiPR00865. HPVCAPSIDL1.
SUPFAMiSSF88648. SSF88648. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiVL1_HPV16
AccessioniPrimary (citable) accession number: P03101
Secondary accession number(s): O00530
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 20, 2016
Last modified: November 30, 2016
This is version 103 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

HPV16, in comparison to HPV types 6 and 11, is more often associated with malignant genital cancers in humans.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.