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Protein

Minor capsid protein VP2

Gene
N/A
Organism
Murine polyomavirus (strain A2) (MPyV)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Isoform VP2 is a structural protein that resides within the core of the capsid surrounded by 72 VP1 pentamers. Participates in host cell receptor binding together with VP1. Following virus endocytosis and trafficking to the endoplasmic reticulum, VP2 and VP3 form oligomers and integrate into the endoplasmic reticulum membrane. Heterooligomer VP2-VP3 may create a viroporin for transporting the viral genome across the endoplasmic reticulum membrane to the cytoplasm. Nuclear entry of the viral DNA involves the selective exposure and importin recognition of VP2 or Vp3 nuclear localization signal (shared C-terminus). Plays a role in virion assembly within the nucleus in particular through a DNA-binding domain located in the C-terminal region. A N-terminal myristoylation suggests a scaffold function for virion assembly (By similarity).By similarity
Isoform VP3: structural protein that resides within the core of the capsid surrounded by 72 VP1 pentamers. Following virus endocytosis and trafficking to the endoplasmic reticulum, VP2 and VP3 form oligomers and integrate into the endoplasmic reticulum membrane. Heterooligomer VP2-VP3 may create a viroporin for transporting the viral genome across the endoplasmic reticulum membrane to the cytoplasm. Nuclear entry of the viral DNA involves the selective exposure and importin recognition of VP2 or Vp3 nuclear localization signal (shared C-terminus). Isoform VP3 plays a role in virion assembly within the nucleus (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding
Biological processViral penetration into host nucleus, Virus entry into host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Minor capsid protein VP2
Alternative name(s):
Minor structural protein VP2
OrganismiMurine polyomavirus (strain A2) (MPyV)
Taxonomic identifieri10636 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePolyomaviridae
Virus hostiMus musculus (Mouse) [TaxID: 10090]
Proteomesi
  • UP000008479 Componenti: Genome

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei287 – 307HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Keywords - Cellular componenti

Capsid protein, Host endoplasmic reticulum, Host membrane, Host nucleus, Membrane, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved; by host
ChainiPRO_00000392172 – 319Minor capsid protein VP2Add BLAST318

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycine; by host1 Publication1

Keywords - PTMi

Lipoprotein, Myristate

Expressioni

Keywords - Developmental stagei

Late protein

Interactioni

Subunit structurei

Isoform VP2 forms homooligomers, and heterooligomers with VP3 in the endoplasmic reticulum membrane. Isoform VP2 interacts (via D1 domain) with VP1. Isoform VP3 interacts (via D1 domain) with VP1 (By similarity).By similarity

Protein-protein interaction databases

DIPiDIP-1089N.
IntActiP03096. 2 interactors.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni266 – 301D1By similarityAdd BLAST36
Regioni306 – 319DNA-bindingBy similarityAdd BLAST14

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi311 – 319Nuclear localization signalBy similarity9

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

OrthoDBiVOG090000AE.

Family and domain databases

InterProiView protein in InterPro
IPR001070. Polyoma_coat_VP2.
PfamiView protein in Pfam
PF00761. Polyoma_coat2. 1 hit.
PIRSFiPIRSF003377. Polyoma_coat2. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing and alternative initiation. AlignAdd to basket

Isoform VP2 (identifier: P03096-1) [UniParc]FASTAAdd to basket
Also known as: Minor capsid protein VP2

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGAALTILVD LIEGLAEVST LTGLSAEAIL SGEALAALDG EITALTLEGV
60 70 80 90 100
MSSETALATM GISEEVYGFV STVPVFVSRT AGAIWLMQTV QGASTISLGI
110 120 130 140 150
QRYLHNEEVP TVNRNMALIP WRDPALLDIY FPGVNQFAHA LNVVHDWGHG
160 170 180 190 200
LLHSVGRYVW QMVVQETQHR LEGAVRELTV RQTHTFLDGL ARLLENTRWV
210 220 230 240 250
VSNAPQSAID AINRGASSAS SGYSSLSDYY RQLGLNPPQR RALFNRIEGS
260 270 280 290 300
MGNGGPTPAA HIQDESGEVI KFYQAQVVSH QRVTPDWMLP LILGLYGDIT
310
PTWATVIEED GPQKKKRRL
Note: Produced by alternative splicing of the late mRNA.
Length:319
Mass (Da):34,801
Last modified:January 23, 2007 - v3
Checksum:iF7168DBCC7A8654C
GO
Isoform VP3 (identifier: P03096-2) [UniParc]FASTAAdd to basket
Also known as: Minor capsid protein VP3

The sequence of this isoform differs from the canonical sequence as follows:
     1-115: Missing.

Note: Produced by alternative initiation at Met-116 of isoform VP2.
Show »
Length:204
Mass (Da):22,892
Checksum:i8A13184798745A44
GO
Isoform VP1 (identifier: P03090-1) [UniParc]FASTAAdd to basket
The sequence of this isoform can be found in the external entry P03090.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Note: Produced by alternative splicing of the late mRNA.
Length:383
Mass (Da):42,452
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0189221 – 115Missing in isoform VP3. CuratedAdd BLAST115

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02288 Genomic DNA. Translation: AAB59903.1.
J02288 Genomic DNA. Translation: AAB59904.1.
PIRiA03635. VVVP2.

Keywords - Coding sequence diversityi

Alternative initiation, Alternative splicing

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiVP2_POVMA
AccessioniPrimary (citable) accession number: P03096
Secondary accession number(s): Q85070
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: June 7, 2017
This is version 91 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families