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Protein

Major capsid protein VP1

Gene
N/A
Organism
Murine polyomavirus (strain 3) (MPyV)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Forms an icosahedral capsid with a T=7 symmetry and a 40 nm diameter. The capsid is composed of 72 pentamers linked to each other by disulfide bonds and associated with VP2 or VP3 proteins. Interacts with terminal alpha(2,3)-linked sialic acids on the cell surface to provide virion attachment to target cell. This attachment induces virion internalization predominantly through caveolin-mediated endocytosis. Once attached, the virion is internalized by caveolin-mediated endocytosis and traffics to the endoplasmic reticulum. Inside the endoplasmic reticulum, the protein folding machinery isomerizes VP1 interpentamer disulfide bonds, thereby triggering initial uncoating. Next, the virion uses the endoplasmic reticulum-associated degradation machinery to probably translocate in the cytosol before reaching the nucleus. Nuclear entry of the viral DNA involves the selective exposure and importin recognition of VP2/Vp3 nuclear localization signal. In late phase of infection, neo-synthesized VP1 encapsulates replicated genomic DNA in the nucleus, and participates in rearranging nucleosomes around the viral DNA (By similarity).By similarity1 Publication

Miscellaneous

The virus attaches to the surface of cells by recognition of oligosaccharides terminating in alpha(2,3)-linked sialic acid. Strains that have Gly-92 (small-plaque strains) can also recognize branched oligosaccharides that carry a second alpha(2,6)-linked sialic acid.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processCaveolin-mediated endocytosis of virus by host, Host-virus interaction, Viral attachment to host cell, Viral penetration into host cytoplasm, Virus endocytosis by host, Virus entry into host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Major capsid protein VP1
Alternative name(s):
Major structural protein VP1
OrganismiMurine polyomavirus (strain 3) (MPyV)
Taxonomic identifieri10635 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePolyomaviridae
Virus hostiMus musculus (Mouse) [TaxID: 10090]

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Capsid protein, Host nucleus, T=7 icosahedral capsid protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved; by hostBy similarity
ChainiPRO_00001150232 – 384Major capsid protein VP1Add BLAST383

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi12Interchain (with C-12)By similarity
Disulfide bondi115Interchain (with C-115)By similarity
Modified residuei358Phosphothreonine; by hostBy similarity1

Keywords - PTMi

Disulfide bond, Phosphoprotein

Expressioni

Keywords - Developmental stagei

Late protein

Interactioni

Subunit structurei

Homomultimer; disulfide-linked. The virus capsid is composed of 72 icosahedral units, each one composed of five disulfide-linked copies of VP1. Interacts with minor capsid proteins VP2 and VP3 (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliP03091.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni322 – 384Intrinsically disorderedBy similarityAdd BLAST63

Domaini

The intrinsically disordered C-terminal region interacts with neighboring pentamers. The unstructured nature of this region allows to make different interactions depending on the structural context: pentamers present at the 12 icosahedral fivefold axes bind five pentamers, when pentamers present at the 60 icosahedral six-fold axes interacts with six pentamers (By similarity).By similarity

Sequence similaritiesi

Family and domain databases

Gene3Di2.60.175.10. 1 hit.
InterProiView protein in InterPro
IPR000662. Capsid_VP1_Polyomavir.
IPR011222. dsDNA_vir_gr_I_capsid.
PfamiView protein in Pfam
PF00718. Polyoma_coat. 1 hit.
PIRSFiPIRSF003376. Capsid_VP1_Polyomavir. 1 hit.
SUPFAMiSSF88648. SSF88648. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform VP1 (identifier: P03091-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAPKRKSGVS KCETKCTKAC PRPAPVPKLL IKGGMEVLDL VTGPDSVTEI
60 70 80 90 100
EAFLNPRMGQ PPTPESLTEG GQYYGWSRGI NLATSDTEDS PENNTLPTWS
110 120 130 140 150
MAKLQLPMLN EDLTCDTLQM WEAVSVKTEV VGSGSLLDVH GFNKPTDTVN
160 170 180 190 200
TKVISTPVEG SQYHVFAVGG EPLDLQGLVT DARTKYKEEG VVTIKTITKK
210 220 230 240 250
DMVNKDQVLN PISKAKLDKD GMYPVEIWHP DPAKNENTRY FGNYTGGTTT
260 270 280 290 300
PPVLQFTNTL TTVLLDENGV GPLCKGEGLY LSCVDIMGRR VTRNYDVHHW
310 320 330 340 350
RGLPRYFKIT LRKRWVKNPY PMASLISSLF NNMLPQVQGQ PMEGENTQVE
360 370 380
EVRVYDGTEP VPGDPDMTRY VDRFGKTKTV FPGN
Note: Produced by alternative splicing of the late mRNA.
Length:384
Mass (Da):42,590
Last modified:January 23, 2007 - v3
Checksum:iB68CEED77A86CBF9
GO
Isoform VP2 (identifier: P03097-1) [UniParc]FASTAAdd to basket
Also known as: Minor capsid protein VP2
The sequence of this isoform can be found in the external entry P03097.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Note: Produced by alternative splicing of the late mRNA.
Length:319
Mass (Da):34,724
GO
Isoform VP3 (identifier: P03097-2) [UniParc]FASTAAdd to basket
Also known as: Minor capsid protein VP3
The sequence of this isoform can be found in the external entry P03097.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Note: Produced by alternative initiation at Met-116 of isoform VP2.
Length:204
Mass (Da):22,789
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01151 Genomic DNA. Translation: CAA24468.1.
PIRiA03629. VVVP13.

Keywords - Coding sequence diversityi

Alternative initiation, Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiVP1_POVM3
AccessioniPrimary (citable) accession number: P03091
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: July 5, 2017
This is version 85 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families