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P03079

- MT_POVHA

UniProt

P03079 - MT_POVHA

Protein

Middle T antigen

Gene
N/A
Organism
Hamster polyomavirus (HaPyV)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 77 (01 Oct 2014)
      Sequence version 1 (21 Jul 1986)
      Previous versions | rss
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    Functioni

    Plays a role in transformation by modulating the activities of cellular proteins involved in control of cell proliferation and by acting as a functional homolog of an activated tyrosine kinase-associated growth-factor receptor. Recruits upon association with Ppp2/PP2A the Src tyrosine kinase component Fyn, thereby activating its kinase activity. In turn, MT becomes phosphorylated and mediates signal transduction pathways leading to cell cycle progression and cell division. MT plays also a role in regulation of early and late gene expression as well as viral DNA replication By similarity.By similarity

    GO - Biological processi

    1. viral process Source: UniProtKB-KW

    Keywords - Biological processi

    Host-virus interaction

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Middle T antigen
    Short name:
    MT
    Short name:
    MT-AG
    OrganismiHamster polyomavirus (HaPyV)
    Taxonomic identifieri10626 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePolyomaviridaePolyomavirus
    Virus hostiMesocricetus auratus (Golden hamster) [TaxID: 10036]
    ProteomesiUP000008477: Genome

    Subcellular locationi

    GO - Cellular componenti

    1. host cell membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Host membrane, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi324 – 3241Y → F: 70% loss of interaction with host Fyn. 1 Publication

    Keywords - Diseasei

    Oncogene

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 401401Middle T antigenPRO_0000115047Add
    BLAST

    Keywords - PTMi

    Phosphoprotein

    Expressioni

    Keywords - Developmental stagei

    Early protein

    Interactioni

    Subunit structurei

    Interacts with host Ppp2/PP2A A and C subunits; this interaction alters PP2A substrate specificity and localization By similarity. Interacts with host Fyn.By similarity1 Publication

    Protein-protein interaction databases

    IntActiP03079. 1 interaction.
    MINTiMINT-8019810.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1AOTNMR-P321-331[»]
    1AOUNMR-P321-331[»]
    1LCJX-ray1.80B321-331[»]
    1SPSX-ray2.70D/E/F321-331[»]
    ProteinModelPortaliP03079.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP03079.

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 374374CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini396 – 4016ExtracellularSequence Analysis

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei375 – 39521HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini12 – 7564JPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 J domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Family and domain databases

    Gene3Di1.10.287.110. 1 hit.
    InterProiIPR001623. DnaJ_domain.
    IPR003354. Papo_T_antigen.
    [Graphical view]
    PfamiPF00226. DnaJ. 1 hit.
    PF02380. Papo_T_antigen. 1 hit.
    [Graphical view]
    SMARTiSM00271. DnaJ. 1 hit.
    [Graphical view]
    SUPFAMiSSF46565. SSF46565. 1 hit.
    PROSITEiPS50076. DNAJ_2. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform Middle T antigen (identifier: P03079-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MDRILTKEEK QALISLLDLE PQYWGDYGRM QKCYKKKCLQ LHPDKGGNEE    50
    LMQQLNTLWT KLKDGLYRVR LLLGPSQVRR LGKDQWNLSL QQTFSGTYFR 100
    RLCRLPITCL RNKGISTCNC ILCLLRKQHF LLKKSWRVPC LVLGECYCID 150
    CFALWFGLPV TNMLVPLYAQ FLAPIPVDWL DLNVHEVYNP ASGMTLMLPP 200
    PPADPESSTI LTQEDTGPTL MGQQDTLTSR RNTGKSFSLS GMLMRTSPAK 250
    KSYHHQKMNS PPGIPIPPPP LFLFPVTAPV PPVTRNTQET QAERENEYMP 300
    MAPQIHLYSQ IREPTHQEEE EPQYEEIPIY LELLPENPNQ HLALTSTARR 350
    SLRRKYHKHN SHIITQRQRN RLRRLVLMIF LLSLGGFFLT LFFLIKRKMH 400
    L 401
    Length:401
    Mass (Da):46,562
    Last modified:July 21, 1986 - v1
    Checksum:i91B6172F4293F448
    GO
    Isoform Small t antigen (identifier: P03080-1) [UniParc]FASTAAdd to Basket

    The sequence of this isoform can be found in the external entry P03080.
    Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
    Length:194
    Mass (Da):22,625
    GO
    Isoform Large T antigen (identifier: P03075-1) [UniParc]FASTAAdd to Basket

    The sequence of this isoform can be found in the external entry P03075.
    Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
    Length:751
    Mass (Da):85,988
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M26281 Genomic DNA. Translation: AAA67117.1.
    X02449 Genomic DNA. Translation: CAB59362.1.
    PIRiA03615. TVVPMH.
    RefSeqiNP_056731.1. NC_001663.1. [P03079-1]

    Genome annotation databases

    GeneIDi1489030.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M26281 Genomic DNA. Translation: AAA67117.1 .
    X02449 Genomic DNA. Translation: CAB59362.1 .
    PIRi A03615. TVVPMH.
    RefSeqi NP_056731.1. NC_001663.1. [P03079-1 ]

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1AOT NMR - P 321-331 [» ]
    1AOU NMR - P 321-331 [» ]
    1LCJ X-ray 1.80 B 321-331 [» ]
    1SPS X-ray 2.70 D/E/F 321-331 [» ]
    ProteinModelPortali P03079.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P03079. 1 interaction.
    MINTi MINT-8019810.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 1489030.

    Miscellaneous databases

    EvolutionaryTracei P03079.

    Family and domain databases

    Gene3Di 1.10.287.110. 1 hit.
    InterProi IPR001623. DnaJ_domain.
    IPR003354. Papo_T_antigen.
    [Graphical view ]
    Pfami PF00226. DnaJ. 1 hit.
    PF02380. Papo_T_antigen. 1 hit.
    [Graphical view ]
    SMARTi SM00271. DnaJ. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46565. SSF46565. 1 hit.
    PROSITEi PS50076. DNAJ_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A new member of the polyomavirus family: the hamster papovavirus. Complete nucleotide sequence and transformation properties."
      Delmas V., Bastien C., Scherneck S., Feunteun J.
      EMBO J. 4:1279-1286(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Functional interaction between the SH2 domain of Fyn and tyrosine 324 of hamster polyomavirus middle-T antigen."
      Dunant N.M., Messerschmitt A.S., Ballmer-Hofer K.
      J. Virol. 71:199-206(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS OF TYR-324, INTERACTION WITH HOST FYN.
    3. "The SH2 domain from the tyrosine kinase Fyn in complex with a phosphotyrosyl peptide reveals insights into domain stability and binding specificity."
      Mulhern T.D., Shaw G.L., Morton C.J., Day A.J., Campbell I.D.
      Structure 5:1313-1323(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF 321-331.
    4. "Recognition of a high-affinity phosphotyrosyl peptide by the Src homology-2 domain of p56lck."
      Eck M.J., Shoelson S.E., Harrison S.C.
      Nature 362:87-91(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 321-331.
    5. "Binding of a high affinity phosphotyrosyl peptide to the Src SH2 domain: crystal structures of the complexed and peptide-free forms."
      Waksman G., Shoelson S.E., Pant N., Cowburn D., Kuriyan J.
      Cell 72:779-790(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 321-331.

    Entry informationi

    Entry nameiMT_POVHA
    AccessioniPrimary (citable) accession number: P03079
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: July 21, 1986
    Last modified: October 1, 2014
    This is version 77 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3