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Protein

Middle T antigen

Gene
N/A
Organism
Murine polyomavirus (strain A2) (MPyV)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in transformation by modulating the activities of cellular proteins involved in control of cell proliferation and by acting as a functional homolog of an activated tyrosine kinase-associated growth-factor receptor. Recruits upon association with host Ppp2/PP2A the Src tyrosine kinase components Src, Yes and Fyn, thereby activating their kinase activity. Activation of Shc1, Pclg1 and p85 mediate signal transduction pathways leading to cell cycle progression and cell division. MT plays also a role in regulation of early and late gene expression and in viral DNA replication.1 Publication

GO - Biological processi

Keywordsi

Biological processHost-virus interaction

Names & Taxonomyi

Protein namesi
Recommended name:
Middle T antigen
Short name:
MT
Short name:
MT-AG
OrganismiMurine polyomavirus (strain A2) (MPyV)
Taxonomic identifieri10636 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePolyomaviridae
Virus hostiMus musculus (Mouse) [TaxID: 10090]
Proteomesi
  • UP000008479 Componenti: Genome

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 394CytoplasmicSequence analysisAdd BLAST394
Transmembranei395 – 415HelicalSequence analysisAdd BLAST21
Topological domaini416 – 421ExtracellularSequence analysis6

GO - Cellular componenti

Keywords - Cellular componenti

Host membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi160T → A: Complete loss of transformation ability. 1 Publication1
Mutagenesisi248P → H: Complete loss of association with host Shc1. 1 Publication1
Mutagenesisi250Y → F: Complete loss of association with host Shc1. 1 Publication1
Mutagenesisi257S → A: Complete loss of interaction with host Ywhaz. 1 Publication1
Mutagenesisi315Y → F: 90% loss of association with host p85. 1 Publication1
Mutagenesisi322Y → F: Complete loss of association with host Plcg1. 1 Publication1

Keywords - Diseasei

Oncogene

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001150491 – 421Middle T antigenAdd BLAST421

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei250Phosphotyrosine; by hostBy similarity1
Modified residuei257Phosphoserine; by hostBy similarity1
Modified residuei315Phosphotyrosine; by hostBy similarity1
Modified residuei322Phosphotyrosine; by hostBy similarity1

Post-translational modificationi

Tyrosine-phosphorylated on three residues 250, 315 and 322, providing docking sites for host Shc1, p85, and Plcg1, respectively.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP03077.

Expressioni

Keywords - Developmental stagei

Early protein

Interactioni

Subunit structurei

Interacts with host Ppp2/PP2A A and C subunits; this interaction alters Ppp2/PP2A substrate specificity and localization. Interacts with host Src, Yes1, and Fyn. Interacts with host Shc1, Plcg1 and p85; these interactions lead to cell cycle progression. Interacts with host 14-3-3 proteins.7 Publications

Protein-protein interaction databases

ELMiP03077.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1YGUX-ray2.90C/D248-251[»]
ProteinModelPortaliP03077.
SMRiP03077.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP03077.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini12 – 75JAdd BLAST64

Domaini

The NPTY motif is required for interaction with host Shc1 protein.

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

OrthoDBiVOG0900002Y.

Family and domain databases

Gene3Di1.10.287.110. 1 hit.
InterProiView protein in InterPro
IPR001623. DnaJ_domain.
IPR003354. Papo_T_antigen.
PfamiView protein in Pfam
PF02380. Papo_T_antigen. 1 hit.
SMARTiView protein in SMART
SM00271. DnaJ. 1 hit.
SUPFAMiSSF161240. SSF161240. 1 hit.
SSF46565. SSF46565. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Middle T antigen (identifier: P03077-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDRVLSRADK ERLLELLKLP RQLWGDFGRM QQAYKQQSLL LHPDKGGSHA
60 70 80 90 100
LMQELNSLWG TFKTEVYNLR MNLGGTGFQV RRLHADGWNL STKDTFGDRY
110 120 130 140 150
YQRFCRMPLT CLVNVKYSSC SCILCLLRKQ HRELKDKCDA RCLVLGECFC
160 170 180 190 200
LECYMQWFGT PTRDVLNLYA DFIASMPIDW LDLDVHSVYN PKRRSEELRR
210 220 230 240 250
AATVHYTMTT GHSAMEASTS QGNGMISSES GTPATSRRLR LPSLLSNPTY
260 270 280 290 300
SVMRSHSYPP TRVLQQIHPH ILLEEDEILV LLSPMTAYPR TPPELLYPES
310 320 330 340 350
DQDQLEPLEE EEEEYMPMED LYLDILPGEQ VPQLIPPPII PRAGLSPWEG
360 370 380 390 400
LILRDLQRAH FDPILDASQR MRATHRAALR AHSMQRHLRR LGRTLLLVTF
410 420
LAALLGICLM LFILIKRSRH F
Length:421
Mass (Da):48,622
Last modified:July 21, 1986 - v1
Checksum:iCA0C25C4984CACB7
GO
Isoform Small t antigen (identifier: P68835-1) [UniParc]FASTAAdd to basket
The sequence of this isoform can be found in the external entry P68835.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:195
Mass (Da):22,811
GO
Isoform Large T antigen (identifier: P03073-1) [UniParc]FASTAAdd to basket
The sequence of this isoform can be found in the external entry P03073.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:785
Mass (Da):88,091
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02288 Genomic DNA. Translation: AAB59900.1.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiMT_POVMA
AccessioniPrimary (citable) accession number: P03077
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: August 30, 2017
This is version 84 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references