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Protein

Middle T antigen

Gene
N/A
Organism
Murine polyomavirus (strain 3) (MPyV)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in transformation by modulating the activities of cellular proteins involved in control of cell proliferation and by acting as a functional homolog of an activated tyrosine kinase-associated growth-factor receptor. Recruits upon association with host Ppp2/PP2A the Src tyrosine kinase components Src, Yes and Fyn, thereby activating their kinase activity. Activation of Shc1, Pclg1 and p85 mediate signal transduction pathways leading to cell cycle progression and cell division. MT plays also a role in regulation of early and late gene expression and in viral DNA replication.

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Host-virus interaction

Names & Taxonomyi

Protein namesi
Recommended name:
Middle T antigen
Short name:
MT
Short name:
MT-AG
OrganismiMurine polyomavirus (strain 3) (MPyV)
Taxonomic identifieri10635 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePolyomaviridaePolyomavirus
Virus hostiMus musculus (Mouse) [TaxID: 10090]
Proteomesi
  • UP000006847 Componenti: Genome

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 394394CytoplasmicSequence analysisAdd
BLAST
Transmembranei395 – 41521HelicalSequence analysisAdd
BLAST
Topological domaini416 – 4216ExtracellularSequence analysis

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host membrane, Membrane

Pathology & Biotechi

Keywords - Diseasei

Oncogene

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 421421Middle T antigenPRO_0000115048Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei250 – 2501Phosphotyrosine; by hostBy similarity
Modified residuei257 – 2571Phosphoserine; by hostBy similarity
Modified residuei315 – 3151Phosphotyrosine; by hostBy similarity
Modified residuei322 – 3221Phosphotyrosine; by hostBy similarity

Post-translational modificationi

Tyrosine-phosphorylated on three residues 250, 315 and 322, providing docking sites for host Shc1, p85, and Plcg1, respectively.

Keywords - PTMi

Phosphoprotein

Expressioni

Keywords - Developmental stagei

Early protein

Interactioni

Subunit structurei

Interacts with host Ppp2/PP2A A and C subunits; this interaction alters Ppp2/PP2A substrate specificity and localization. Interacts with host Src, Yes1, and Fyn. Interacts with host Shc1, Plcg1 and p85; these interactions lead to cell cycle progression. Interacts with host 14-3-3 proteins.

Protein-protein interaction databases

IntActiP03076. 8 interactions.
MINTiMINT-6376645.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1FU5NMR-B312-326[»]
1QJBX-ray2.00Q/S254-259[»]
ProteinModelPortaliP03076.
SMRiP03076. Positions 1-79.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP03076.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini12 – 7564JAdd
BLAST

Domaini

The NPTY motif is required for interaction with host Shc1 protein.

Sequence similaritiesi

Contains 1 J domain.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Family and domain databases

Gene3Di1.10.287.110. 1 hit.
InterProiIPR001623. DnaJ_domain.
IPR003354. Papo_T_antigen.
[Graphical view]
PfamiPF02380. Papo_T_antigen. 1 hit.
[Graphical view]
SMARTiSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Middle T antigen (identifier: P03076-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDRVLSRADK ERLLELLKLP RQLWGDFGRM QQAYKQQSLL LHPDKGGSHA
60 70 80 90 100
LMQELNSLWG TFKTEVYNLR MNLGGTGFQV RRLHADGWNL STKDTFGDRY
110 120 130 140 150
YQRFCRMPLT CLVNVKYSSC SCILCLLRKQ HRELKDKCDA RCLVLGECFC
160 170 180 190 200
LECYMQWFGT PTRDVLNLYA DFIASMPIDW LDLDVHSVYN PKRRSEELRR
210 220 230 240 250
AATVHYTMTT GHSAMEASTS QGNGMISSES GTPATSRRLR LPSLLSNPTY
260 270 280 290 300
SVMRSHSYPP TRVLQQIHPH ILLEEDEILV LLSPMTAYPR TPPELLYPES
310 320 330 340 350
DQDQLEPLEE EEEEYMPMED LYLDILPEEQ VPQLIPPPII PRAGLSPWEG
360 370 380 390 400
LILRDLQRAH FDPILDASQR MRATHRAALR AHSMQRHLRR LGRTLLLVTF
410 420
LAALLGICLM LFILIKRSRH F
Length:421
Mass (Da):48,694
Last modified:July 10, 2007 - v2
Checksum:iFC394691F84E8EB5
GO
Isoform Small t antigen (identifier: P68834-1) [UniParc]FASTAAdd to basket

The sequence of this isoform can be found in the external entry P68834.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:195
Mass (Da):22,811
GO
Isoform Large T antigen (identifier: P03074-1) [UniParc]FASTAAdd to basket

The sequence of this isoform can be found in the external entry P03074.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:782
Mass (Da):87,662
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U27812 Genomic DNA. Translation: AAA93240.1.
U27813 Genomic DNA. Translation: AAA93246.1.
J02289 Genomic DNA. Translation: AAA46873.1.
AF442959 Genomic DNA. Translation: AAL35608.1.
PIRiA93718. TVVPM.
RefSeqiYP_009111414.1. NC_001515.2.

Genome annotation databases

GeneIDi22619583.
KEGGivg:22619583.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U27812 Genomic DNA. Translation: AAA93240.1.
U27813 Genomic DNA. Translation: AAA93246.1.
J02289 Genomic DNA. Translation: AAA46873.1.
AF442959 Genomic DNA. Translation: AAL35608.1.
PIRiA93718. TVVPM.
RefSeqiYP_009111414.1. NC_001515.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1FU5NMR-B312-326[»]
1QJBX-ray2.00Q/S254-259[»]
ProteinModelPortaliP03076.
SMRiP03076. Positions 1-79.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP03076. 8 interactions.
MINTiMINT-6376645.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi22619583.
KEGGivg:22619583.

Miscellaneous databases

EvolutionaryTraceiP03076.

Family and domain databases

Gene3Di1.10.287.110. 1 hit.
InterProiIPR001623. DnaJ_domain.
IPR003354. Papo_T_antigen.
[Graphical view]
PfamiPF02380. Papo_T_antigen. 1 hit.
[Graphical view]
SMARTiSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The nucleotide sequence and restriction enzyme sites of the polyoma genome."
    Deininger P.L., Esty A., LaPorte P., Hsu H., Friedmann T.
    Nucleic Acids Res. 8:855-860(1980) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Genetic and structural analysis of a virulence determinant in polyomavirus VP1."
    Bauer P.H., Bronson R.T., Fung S.C., Freund R., Stehle T., Harrison S.C., Benjamin T.L.
    J. Virol. 69:7925-7931(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Isolate LID and Isolate PTA.
  3. Clark B.E., Griffin B.E.
    Submitted (NOV-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: BG.
  4. "NMR structure of the N-SH2 of the p85 subunit of phosphoinositide 3-kinase complexed to a doubly phosphorylated peptide reveals a second phosphotyrosine binding site."
    Weber T., Schaffhausen B., Liu Y., Gunther U.L.
    Biochemistry 39:15860-15869(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 331-345.

Entry informationi

Entry nameiMT_POVM3
AccessioniPrimary (citable) accession number: P03076
Secondary accession number(s): Q76TX4, Q76W01, Q89765
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 10, 2007
Last modified: December 9, 2015
This is version 78 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.