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P03070

- LT_SV40

UniProt

P03070 - LT_SV40

Protein

Large T antigen

Gene
N/A
Organism
Simian virus 40 (SV40)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 136 (01 Oct 2014)
      Sequence version 2 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    Isoform large T antigen is a key early protein essential for both driving viral replication and inducing cellular transformation. Plays a role in viral genome replication by driving entry of quiescent cells into the cell cycle and by autoregulating the synthesis of viral early mRNA. Displays highly oncogenic activities by corrupting the host cellular checkpoint mechanisms that guard cell division and the transcription, replication, and repair of DNA. Participates in the modulation of cellular gene expression preceeding viral DNA replication. This step involves binding to host key cell cycle regulators retinoblastoma protein RB1/pRb and TP53. Induces the disassembly of host E2F1 transcription factors from RB1, thus promoting transcriptional activation of E2F1-regulated S-phase genes. Inhibits host TP53 binding to DNA, abrogating the ability of TP53 to stimulate gene expression. Plays the role of a TFIID-associated factor (TAF) in transcription initiation for all three RNA polymerases, by stabilizing the TBP-TFIIA complex on promoters. Initiates viral DNA replication and unwinding via interactions with the viral origin of replication. Binds two adjacent sites in the SV40 origin. The replication fork movement is facilitated by Large T antigen helicase activity. Activates the transcription of viral late mRNA, through host TBP and TFIIA stabilization. Interferes with histone deacetylation mediated by HDAC1, leading to activation of transcription. May inactivate the growth-suppressing properties of the E3 ubiquitin ligase CUL7.
    Isoform 17kT antigen targets host RBL2 for degradation and promotes cell proliferation. Transactivates host cyclin A promoter through its J domain.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi139 – 254116T-ag OBDPROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri265 – 35793T-ag D1-typePROSITE-ProRule annotationAdd
    BLAST
    Nucleotide bindingi426 – 4338ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. DNA replication origin binding Source: InterPro
    3. helicase activity Source: UniProtKB-KW
    4. metal ion binding Source: UniProtKB-KW
    5. protein binding Source: UniProtKB

    GO - Biological processi

    1. DNA replication Source: UniProtKB-KW
    2. modulation by virus of host G1/S transition checkpoint Source: UniProtKB-KW
    3. modulation by virus of host ubiquitin-protein ligase activity Source: UniProtKB-KW
    4. regulation of transcription, DNA-templated Source: UniProtKB-KW
    5. suppression by virus of host JAK1 activity Source: UniProtKB-KW
    6. suppression by virus of host type I interferon-mediated signaling pathway Source: UniProtKB-KW
    7. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator, Helicase, Hydrolase

    Keywords - Biological processi

    DNA replication, G1/S host cell cycle checkpoint dysregulation by virus, Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host interferon signaling pathway by virus, Inhibition of host JAK1 by virus, Modulation of host cell cycle by virus, Modulation of host E3 ubiquitin ligases by virus, Modulation of host ubiquitin pathway by virus, Transcription, Transcription regulation, Viral immunoevasion

    Keywords - Ligandi

    ATP-binding, DNA-binding, Metal-binding, Nucleotide-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Large T antigen (EC:3.6.4.-)
    Short name:
    LT
    Short name:
    LT-AG
    OrganismiSimian virus 40 (SV40)
    Taxonomic identifieri10633 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePolyomaviridaePolyomavirus
    Virus hostiMacaca (macaques) [TaxID: 9539]
    ProteomesiUP000007705: Genome

    Subcellular locationi

    Host nucleus 1 Publication

    GO - Cellular componenti

    1. host cell nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi98 – 981F → A: Complete loss of interaction with host CUL7. 1 Publication
    Mutagenesisi124 – 1241T → A: 200-fold reduction in phosphorylation by CDC2. No DNA replication activation. 1 Publication
    Mutagenesisi679 – 6791S → A: Enhanced DNA replication.
    Mutagenesisi701 – 7011T → A: Complete loss of interaction with host FBW7gamma isoform. 1 Publication

    Keywords - Diseasei

    Oncogene

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 708708Large T antigenPRO_0000115046Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionine; by host2 Publications
    Modified residuei106 – 1061Phosphoserine; by host
    Modified residuei112 – 1121Phosphoserine; by host
    Modified residuei120 – 1201Phosphoserine; by host
    Modified residuei123 – 1231Phosphoserine; by host
    Modified residuei124 – 1241Phosphothreonine; by host2 Publications
    Modified residuei639 – 6391Phosphoserine; by host
    Modified residuei676 – 6761Phosphoserine; by host
    Modified residuei677 – 6771Phosphoserine; by host
    Modified residuei679 – 6791Phosphoserine; by host
    Modified residuei697 – 6971N6-acetyllysine; by host1 Publication
    Modified residuei701 – 7011Phosphothreonine; by host

    Post-translational modificationi

    Phosphorylated on both serine and threonine residues. Phosphorylation on Ser-120 and Ser-123 inhibits viral replication, while phosphorylation on Thr-124 enhances replication by activating the DNA-binding domain. Phosphorylation on Thr-701 is required for binding to host FBW7gamma isoform. Dephosphorylated preferentially by PP2A on Ser-120, Ser-123, Ser-677 and perhaps Ser-679. Small t antigen inhibits the dephosphorylation by the AC form of PP2A.4 Publications
    O-Glycosylated near the C-terminal region.1 Publication
    Acetylated by CBP in a TP53-dependent manner.2 Publications

    Keywords - PTMi

    Acetylation, Glycoprotein, Phosphoprotein

    Proteomic databases

    PRIDEiP03070.

    PTM databases

    UniCarbKBiP03070.

    Expressioni

    Keywords - Developmental stagei

    Early protein

    Interactioni

    Subunit structurei

    Isoform large T antigen forms homohexamers in the presence of ATP. Interacts with host HDAC1. Interacts (via LXCXE domain) with host RB1; the interaction induces the aberrant dissociation of RB1-E2F1 complex thereby disrupting RB1's activity. Interacts (via LXCXE domain) with host pRB-related proteins RBL1 and RBL2. Interacts (via C-terminus) with host TOP1 and POLA1 allowing DNA replication. Interacts with host TP53, inhibiting TP53 binding to DNA. Interacts with host preinitiation complex components TBP, TFIIA and TFIID to regulate transcription initiation. LT interacts (via CPD region) with host FBW7gamma isoform (via WD repeats); seems to function as a competitive inhibitor of FBW7gamma function for physiologic substrates. LT interacts with host E3 ubiquitin ligase CUL7; this interaction seems to inhibit CUL7. Component of a SCF(CUL7)-like complex composed of SV40 Lt and host proteins CUL7, SKP1, RBX1, and FBXW8. LT interacts with host BUB1; this interaction induces activation of a DNA damage response and promotes p53 stabilization and phosphorylation Probable. Interacts with host FAM111A and this interaction is required for efficient viral replication and sustained viral gene expression in restrictive cell types.13 PublicationsCurated

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    BUB1O436832EBI-617698,EBI-748936From a different organism.
    CCNA2P202482EBI-617698,EBI-457097From a different organism.
    CDC28P005463EBI-617698,EBI-4253From a different organism.
    CDK1P064932EBI-617698,EBI-444308From a different organism.
    CREBBPQ927932EBI-617698,EBI-81215From a different organism.
    EP300Q094722EBI-617698,EBI-447295From a different organism.
    IRS1P355682EBI-617698,EBI-517592From a different organism.
    Kpna2P522933EBI-617698,EBI-3043908From a different organism.
    MYCP011062EBI-617698,EBI-447544From a different organism.
    POLA1P098846EBI-617698,EBI-850026From a different organism.
    PURAQ005772EBI-617698,EBI-1045860From a different organism.
    RB1P064006EBI-617698,EBI-491274From a different organism.
    RPA1P276943EBI-617698,EBI-621389From a different organism.
    TP53P0463718EBI-617698,EBI-366083From a different organism.
    Tp53P0234012EBI-617698,EBI-474016From a different organism.

    Protein-protein interaction databases

    DIPiDIP-24251N.
    IntActiP03070. 36 interactions.
    MINTiMINT-91005.

    Structurei

    Secondary structure

    1
    708
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi7 – 1610
    Helixi27 – 3610
    Turni37 – 404
    Turni43 – 453
    Turni48 – 525
    Helixi53 – 6715
    Beta strandi87 – 893
    Helixi91 – 10212
    Beta strandi136 – 1383
    Helixi140 – 1456
    Beta strandi156 – 1638
    Helixi165 – 17814
    Beta strandi182 – 1898
    Beta strandi192 – 20312
    Helixi205 – 21511
    Beta strandi217 – 2193
    Beta strandi221 – 2277
    Helixi229 – 2368
    Beta strandi242 – 2487
    Helixi251 – 2544
    Helixi270 – 27910
    Helixi285 – 2939
    Helixi294 – 2963
    Turni299 – 3013
    Helixi303 – 3064
    Helixi311 – 3144
    Helixi317 – 32711
    Helixi333 – 35422
    Helixi357 – 37519
    Beta strandi377 – 3793
    Helixi384 – 39411
    Turni395 – 3973
    Helixi401 – 41414
    Beta strandi421 – 4255
    Beta strandi428 – 4314
    Helixi432 – 44312
    Beta strandi446 – 4483
    Beta strandi450 – 4523
    Turni454 – 4563
    Helixi457 – 4615
    Helixi462 – 4643
    Beta strandi470 – 4723
    Turni479 – 4846
    Helixi490 – 4956
    Helixi498 – 5025
    Beta strandi507 – 5093
    Beta strandi512 – 5143
    Beta strandi517 – 5193
    Beta strandi524 – 5285
    Helixi535 – 5384
    Beta strandi541 – 5466
    Helixi551 – 5588
    Helixi562 – 5654
    Helixi572 – 58211
    Helixi585 – 5873
    Helixi590 – 5923
    Helixi593 – 60614
    Helixi609 – 62113

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1EJLX-ray2.80A/B126-132[»]
    1GH6X-ray3.20A7-117[»]
    1N25X-ray2.80A/B260-627[»]
    1Q1SX-ray2.30A/B110-133[»]
    1Q1TX-ray2.50A/B110-134[»]
    1SVLX-ray1.95A/B/C251-627[»]
    1SVMX-ray1.94A/B/C/D/E/F251-627[»]
    1SVOX-ray2.60A/B251-627[»]
    1TBDNMR-A131-260[»]
    1Z1DNMR-B131-259[»]
    2FUFX-ray1.45A131-259[»]
    2H1LX-ray3.16A/B/C/D/E/F/G/H/I/J/K/L260-627[»]
    2IF9X-ray2.59A/B131-260[»]
    2IPRX-ray1.50A/B131-259[»]
    2ITJX-ray2.50A/B131-259[»]
    2ITLX-ray1.65A/B131-259[»]
    2NL8X-ray2.30A131-259[»]
    2NTCX-ray2.40A/B131-260[»]
    2TBDNMR-A131-260[»]
    3QK2X-ray1.64A131-260[»]
    3QN2X-ray1.66A131-260[»]
    4E2IX-ray5.00A/B/C/D/E/F/G/H/I/J/K/L266-627[»]
    4FGNX-ray3.20A/B131-260[»]
    4GDFX-ray2.80A/B/E/F131-627[»]
    ProteinModelPortaliP03070.
    SMRiP03070. Positions 7-117, 131-260, 265-627.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP03070.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini12 – 7564JPROSITE-ProRule annotationAdd
    BLAST
    Domaini400 – 560161SF3 helicasePROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni63 – 8927Binding of LT to the CUL7 complexAdd
    BLAST
    Regioni337 – 672336Binding to host TP53 proteinAdd
    BLAST
    Regioni418 – 616199ATPase activityAdd
    BLAST
    Regioni627 – 70882C-terminal regionAdd
    BLAST
    Regioni699 – 70810CPD

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi103 – 1075LXCXE motif
    Motifi125 – 1328Nuclear localization signal1 Publication

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi633 – 6386Poly-Asp

    Domaini

    The J domain is essential for multiple viral activities, including virion assembly, viral DNA replication, transformation and transcriptional activation.1 Publication
    The LXCXE motif specifically binds to host pRB, RBL1, and RBL2.
    The origin-binding domain (T-ag OBD) interacts specifically with several pentameric sequences 5'-GAGGC-3' in the SV40 origin of DNA replication.
    The zinc finger region contributes to protein-protein interactions essential for the assembly of stable T-antigen hexamers at the origin of replication. The hexamers are required for subsequent alterations in the structure of origin DNA (PubMed:2173794, PubMed:1851875).2 Publications
    The C-terminal region is involved in interaction with host FAM111A. It is also required for the host range and adenovirus helper functions of the virus (PubMed:23093934).1 Publication
    The ATP binding/ATPase domain is required for proper hexamer assembly and helicase activity.1 Publication
    Cdc4 phospho-degron (CPD) region is involved in interaction with host FBW7gamma isoform.

    Sequence similaritiesi

    Contains 1 J domain.PROSITE-ProRule annotation
    Contains 1 SF3 helicase domain.PROSITE-ProRule annotation
    Contains 1 T-ag D1-type zinc finger.PROSITE-ProRule annotation
    Contains 1 T-ag OBD DNA-binding domain.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri265 – 35793T-ag D1-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Family and domain databases

    Gene3Di1.10.10.510. 1 hit.
    1.10.287.110. 1 hit.
    3.40.50.300. 1 hit.
    InterProiIPR001623. DnaJ_domain.
    IPR014015. Helicase_SF3_DNA-vir.
    IPR016392. Lg_T_Ag_polyomavir.
    IPR010932. Lg_T_Ag_Polyomavir_C.
    IPR027417. P-loop_NTPase.
    IPR003133. T_Ag_DNA-bd.
    IPR017910. Znf_lg_T-Ag_D1-typ.
    [Graphical view]
    PfamiPF00226. DnaJ. 1 hit.
    PF06431. Polyoma_lg_T_C. 1 hit.
    PF02217. T_Ag_DNA_bind. 1 hit.
    [Graphical view]
    PIRSFiPIRSF003368. Large_T_antigen_polyomaV. 1 hit.
    SMARTiSM00271. DnaJ. 1 hit.
    [Graphical view]
    SUPFAMiSSF46565. SSF46565. 1 hit.
    SSF52540. SSF52540. 1 hit.
    PROSITEiPS50076. DNAJ_2. 1 hit.
    PS51206. SF3_HELICASE_1. 1 hit.
    PS51287. T_AG_OBD. 1 hit.
    PS51341. ZF_LTAG_D1. 1 hit.
    [Graphical view]

    Sequences (4)i

    Sequence statusi: Complete.

    This entry describes 4 isoformsi produced by alternative splicing and alternative initiation. Align

    Isoform Large T antigen (identifier: P03070-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MDKVLNREES LQLMDLLGLE RSAWGNIPLM RKAYLKKCKE FHPDKGGDEE    50
    KMKKMNTLYK KMEDGVKYAH QPDFGGFWDA TEIPTYGTDE WEQWWNAFNE 100
    ENLFCSEEMP SSDDEATADS QHSTPPKKKR KVEDPKDFPS ELLSFLSHAV 150
    FSNRTLACFA IYTTKEKAAL LYKKIMEKYS VTFISRHNSY NHNILFFLTP 200
    HRHRVSAINN YAQKLCTFSF LICKGVNKEY LMYSALTRDP FSVIEESLPG 250
    GLKEHDFNPE EAEETKQVSW KLVTEYAMET KCDDVLLLLG MYLEFQYSFE 300
    MCLKCIKKEQ PSHYKYHEKH YANAAIFADS KNQKTICQQA VDTVLAKKRV 350
    DSLQLTREQM LTNRFNDLLD RMDIMFGSTG SADIEEWMAG VAWLHCLLPK 400
    MDSVVYDFLK CMVYNIPKKR YWLFKGPIDS GKTTLAAALL ELCGGKALNV 450
    NLPLDRLNFE LGVAIDQFLV VFEDVKGTGG ESRDLPSGQG INNLDNLRDY 500
    LDGSVKVNLE KKHLNKRTQI FPPGIVTMNE FSVPKTLQAR FVKQIDFRAK 550
    DYLKHCLERS EFLLEKRIIQ SGIALLLMLI WYRPVAEFAQ SIQSRIVEWK 600
    ERLDKEFSLS VYQKMKFNVA MGIGVLDWLR NSDDDDEDSQ ENADKNEDGG 650
    EKNMEDSGHE TGIDSQSQGS FQAPQSSQSV HDHNQPYHIC RGFTCFKKPP 700
    TPPPEPET 708
    Length:708
    Mass (Da):81,582
    Last modified:February 1, 1996 - v2
    Checksum:iCB81306EF9E4E2C0
    GO
    Isoform Small t antigen (identifier: P03081-1) [UniParc]FASTAAdd to Basket

    The sequence of this isoform can be found in the external entry P03081.
    Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

    Note: Produced by alternative splicing.

    Length:174
    Mass (Da):20,449
    GO
    Isoform 17kT antigen (identifier: P03070-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         132-135: VEDP → ALLT
         136-708: Missing.

    Note: Produced by alternative splicing.

    Show »
    Length:135
    Mass (Da):15,754
    Checksum:i34517296D3E87E4F
    GO
    Isoform SELP (identifier: P0C6L2-1) [UniParc]FASTAAdd to Basket

    The sequence of this isoform can be found in the external entry P0C6L2.
    Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

    Note: Produced by alternative initiation.

    Length:23
    Mass (Da):2,705
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti531 – 5311F → Y in strain: 776.
    Natural varianti549 – 5491A → P in strain: 776.
    Natural varianti552 – 5521Y → P in strain: 776.

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei132 – 1354VEDP → ALLT in isoform 17kT antigen. 1 PublicationVSP_035893
    Alternative sequencei136 – 708573Missing in isoform 17kT antigen. 1 PublicationVSP_035894Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J02400 Genomic DNA. Translation: AAB59924.1.
    RefSeqiYP_003708382.1. NC_001669.1.

    Genome annotation databases

    GeneIDi1489531.

    Keywords - Coding sequence diversityi

    Alternative initiation, Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J02400 Genomic DNA. Translation: AAB59924.1 .
    RefSeqi YP_003708382.1. NC_001669.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1EJL X-ray 2.80 A/B 126-132 [» ]
    1GH6 X-ray 3.20 A 7-117 [» ]
    1N25 X-ray 2.80 A/B 260-627 [» ]
    1Q1S X-ray 2.30 A/B 110-133 [» ]
    1Q1T X-ray 2.50 A/B 110-134 [» ]
    1SVL X-ray 1.95 A/B/C 251-627 [» ]
    1SVM X-ray 1.94 A/B/C/D/E/F 251-627 [» ]
    1SVO X-ray 2.60 A/B 251-627 [» ]
    1TBD NMR - A 131-260 [» ]
    1Z1D NMR - B 131-259 [» ]
    2FUF X-ray 1.45 A 131-259 [» ]
    2H1L X-ray 3.16 A/B/C/D/E/F/G/H/I/J/K/L 260-627 [» ]
    2IF9 X-ray 2.59 A/B 131-260 [» ]
    2IPR X-ray 1.50 A/B 131-259 [» ]
    2ITJ X-ray 2.50 A/B 131-259 [» ]
    2ITL X-ray 1.65 A/B 131-259 [» ]
    2NL8 X-ray 2.30 A 131-259 [» ]
    2NTC X-ray 2.40 A/B 131-260 [» ]
    2TBD NMR - A 131-260 [» ]
    3QK2 X-ray 1.64 A 131-260 [» ]
    3QN2 X-ray 1.66 A 131-260 [» ]
    4E2I X-ray 5.00 A/B/C/D/E/F/G/H/I/J/K/L 266-627 [» ]
    4FGN X-ray 3.20 A/B 131-260 [» ]
    4GDF X-ray 2.80 A/B/E/F 131-627 [» ]
    ProteinModelPortali P03070.
    SMRi P03070. Positions 7-117, 131-260, 265-627.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-24251N.
    IntActi P03070. 36 interactions.
    MINTi MINT-91005.

    Chemistry

    ChEMBLi CHEMBL1075257.

    PTM databases

    UniCarbKBi P03070.

    Proteomic databases

    PRIDEi P03070.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 1489531.

    Miscellaneous databases

    EvolutionaryTracei P03070.

    Family and domain databases

    Gene3Di 1.10.10.510. 1 hit.
    1.10.287.110. 1 hit.
    3.40.50.300. 1 hit.
    InterProi IPR001623. DnaJ_domain.
    IPR014015. Helicase_SF3_DNA-vir.
    IPR016392. Lg_T_Ag_polyomavir.
    IPR010932. Lg_T_Ag_Polyomavir_C.
    IPR027417. P-loop_NTPase.
    IPR003133. T_Ag_DNA-bd.
    IPR017910. Znf_lg_T-Ag_D1-typ.
    [Graphical view ]
    Pfami PF00226. DnaJ. 1 hit.
    PF06431. Polyoma_lg_T_C. 1 hit.
    PF02217. T_Ag_DNA_bind. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF003368. Large_T_antigen_polyomaV. 1 hit.
    SMARTi SM00271. DnaJ. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46565. SSF46565. 1 hit.
    SSF52540. SSF52540. 1 hit.
    PROSITEi PS50076. DNAJ_2. 1 hit.
    PS51206. SF3_HELICASE_1. 1 hit.
    PS51287. T_AG_OBD. 1 hit.
    PS51341. ZF_LTAG_D1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ACETYLATION AT MET-1.
      Strain: 776.
    3. "Independent expression of the transforming amino-terminal domain of SV40 large I antigen from an alternatively spliced third SV40 early mRNA."
      Zerrahn J., Knippschild U., Winkler T., Deppert W.
      EMBO J. 12:4739-4746(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE (ISOFORM 17KT ANTIGEN), ALTERNATIVE SPLICING.
    4. "Phosphorylation of large tumour antigen by cdc2 stimulates SV40 DNA replication."
      McVey D., Brizuela L., Mohr I., Marshak D.R., Gluzman Y., Beach D.
      Nature 341:503-507(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 102-118, PHOSPHORYLATION AT THR-124 BY CDC2.
    5. "T antigen is bound to a host protein in SV40-transformed cells."
      Lane D.P., Crawford L.V.
      Nature 278:261-263(1979) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH HOST TP53.
    6. "Topography of simian virus 40 A protein-DNA complexes: arrangement of pentanucleotide interaction sites at the origin of replication."
      DeLucia A.L., Lewton B.A., Tjian R., Tegtmeyer P.
      J. Virol. 46:143-150(1983) [PubMed] [Europe PMC] [Abstract]
      Cited for: DNA-BINDING.
    7. "A short amino acid sequence able to specify nuclear location."
      Kalderon D., Roberts B.L., Richardson W.D., Smith A.E.
      Cell 39:499-509(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEAR LOCALIZATION SIGNAL.
    8. "T-antigen-DNA polymerase alpha complex implicated in simian virus 40 DNA replication."
      Smale S.T., Tjian R.
      Mol. Cell. Biol. 6:4077-4087(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH HOST POLA1.
    9. "Glycosylation of simian virus 40 T antigen and localization of glycosylated T antigen in the nuclear matrix."
      Schmitt M.K., Mann K.
      Virology 156:268-281(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION, SUBCELLULAR LOCATION.
    10. "SV40 large tumor antigen forms a specific complex with the product of the retinoblastoma susceptibility gene."
      Decaprio J.A., Ludlow J.W., Figge J., Shew J.-Y., Huang C.-M., Lee W.-H., Marsilio E., Paucha E., Livingston D.M.
      Cell 54:275-283(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH HOST RB PROTEIN.
    11. Cited for: PHOSPHORYLATION.
    12. "Activation of SV40 DNA replication in vitro by cellular protein phosphatase 2A."
      Virshup D.M., Kauffman M.G., Kelly T.J.
      EMBO J. 8:3891-3898(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACTIVATION OF DNA REPLICATION BY HOST PP2A.
    13. "The cellular 107K protein that binds to adenovirus E1A also associates with the large T antigens of SV40 and JC virus."
      Dyson N., Buchkovich K., Whyte P., Harlow E.
      Cell 58:249-255(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH HOST RBL1.
    14. "The replication functions of SV40 T antigen are regulated by phosphorylation."
      Prives C.
      Cell 61:735-738(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION.
    15. "The finger domain of simian virus 40 large T antigen controls DNA-binding specificity."
      Hoess A., Moarefi I.F., Fanning E., Arthur A.K.
      J. Virol. 64:6291-6296(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: ZINC-FINGER.
    16. "The zinc finger region of simian virus 40 large T antigen is needed for hexamer assembly and origin melting."
      Loeber G., Stenger J.E., Ray S., Parsons R.E., Anderson M.E., Tegtmeyer P.
      J. Virol. 65:3167-3174(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: ZINC-FINGER.
    17. "Dephosphorylation of simian virus 40 large-T antigen and p53 protein by protein phosphatase 2A: inhibition by small-t antigen."
      Scheidtmann K.H., Mumby M.C., Rundell K., Walter G.
      Mol. Cell. Biol. 11:1996-2003(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: DEPHOSPHORYLATION BY HOST PP2A.
    18. "Protein phosphatase 2A dephosphorylates simian virus 40 large T antigen specifically at residues involved in regulation of DNA-binding activity."
      Scheidtmann K.H., Virshup D.M., Kelly T.J.
      J. Virol. 65:2098-2101(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: DEPHOSPHORYLATION BY HOST PP2A.
    19. "Adenovirus E1A, simian virus 40 tumor antigen, and human papillomavirus E7 protein share the capacity to disrupt the interaction between transcription factor E2F and the retinoblastoma gene product."
      Chellappan S., Kraus V.B., Kroger B., Munger K., Howley P.M., Phelps W.C., Nevins J.R.
      Proc. Natl. Acad. Sci. U.S.A. 89:4549-4553(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH HOST RB1.
    20. "Mechanisms of simian virus 40 T-antigen activation by phosphorylation of threonine 124."
      McVey D., Woelker B., Tegtmeyer P.
      J. Virol. 70:3887-3893(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION AT THR-124, MUTAGENESIS OF THR-124.
    21. "TAF-like function of SV40 large T antigen."
      Damania B., Alwine J.C.
      Genes Dev. 10:1369-1381(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH HOST TBP AND TFIID.
    22. "Simian virus 40 large T antigen stabilizes the TATA-binding protein-TFIIA complex on the TATA element."
      Damania B., Lieberman P., Alwine J.C.
      Mol. Cell. Biol. 18:3926-3935(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN TRANSCRIPTION INITIATION, INTERACTION WITH HOST TFIIA.
    23. "The J domain of simian virus 40 large T antigen is required to functionally inactivate RB family proteins."
      Zalvide J., Stubdal H., DeCaprio J.A.
      Mol. Cell. Biol. 18:1408-1415(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: J DOMAIN.
    24. "SV40 large T antigen hexamer structure: domain organization and DNA-induced conformational changes."
      VanLoock M.S., Alexandrov A., Yu X., Cozzarelli N.R., Egelman E.H.
      Curr. Biol. 12:472-476(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: ATPASE REGION.
    25. "p53 targets simian virus 40 large T antigen for acetylation by CBP."
      Poulin D.L., Kung A.L., DeCaprio J.A.
      J. Virol. 78:8245-8253(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION.
    26. "PP2A-dependent transactivation of the cyclin A promoter by SV40 ST is mediated by a cell cycle-regulated E2F site."
      Skoczylas C., Henglein B., Rundell K.
      Virology 332:596-601(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION OF ISOFORM 17KT.
    27. "The SV40 large T antigen contains a decoy phosphodegron that mediates its interactions with Fbw7/hCdc4."
      Welcker M., Clurman B.E.
      J. Biol. Chem. 280:7654-7658(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH HUMAN FBW7GAMMA ISOFORM, MUTAGENESIS OF THR-701.
    28. "Simian virus 40 large T antigen's association with the CUL7 SCF complex contributes to cellular transformation."
      Kasper J.S., Kuwabara H., Arai T., Ali S.H., DeCaprio J.A.
      J. Virol. 79:11685-11692(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH HUMAN CUL7, IDENTIFICATION IN A SCF(CUL7)-LIKE COMPLEX, MUTAGENESIS OF PHE-98.
    29. "Involvement of chromatin and histone deacetylation in SV40 T antigen transcription regulation."
      Valls E., Blanco-Garcia N., Aquizu N., Piedra D., Estaras C., de la Cruz X., Martinez-Balbas M.A.
      Nucleic Acids Res. 35:1958-1968(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH HOST HDAC1.
    30. "Simian virus 40 DNA replication is dependent on an interaction between topoisomerase I and the C-terminal end of T antigen."
      Khopde S., Simmons D.T.
      J. Virol. 82:1136-1145(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH HOST TOP1.
    31. "Simian virus 40 large T antigen disrupts genome integrity and activates a DNA damage response via Bub1 binding."
      Hein J., Boichuk S., Wu J., Cheng Y., Freire R., Jat P.S., Roberts T.M., Gjoerup O.V.
      J. Virol. 83:117-127(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH HUMAN BUB1.
    32. Cited for: INTERACTION WITH HOST FAM111A, C-TERMINAL REGION.
    33. "Structural basis for the inactivation of retinoblastoma tumor suppressor by SV40 large T antigen."
      Kim H.-Y., Ahn B.-Y., Cho Y.
      EMBO J. 20:295-304(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.2 ANGSTROMS) OF 7-117.
    34. "Structure of the replicative helicase of the oncoprotein SV40 large tumour antigen."
      Li D., Zhao R., Lilyestrom W., Gai D., Zhang R., DeCaprio J.A., Fanning E., Jochimiak A., Szakonyi G., Chen X.S.
      Nature 423:512-518(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 260-627.
    35. "Mechanisms of conformational change for a replicative hexameric helicase of SV40 large tumor antigen."
      Gai D., Zhao R., Li D., Finkielstein C.V., Chen X.S.
      Cell 119:47-60(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS) OF 251-627.
    36. "Crystal structure of SV40 large T-antigen bound to p53: interplay between a viral oncoprotein and a cellular tumor suppressor."
      Lilyestrom W., Klein M.G., Zhang R., Joachimiak A., Chen X.S.
      Genes Dev. 20:2373-2382(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.16 ANGSTROMS) OF 251-627.

    Entry informationi

    Entry nameiLT_SV40
    AccessioniPrimary (citable) accession number: P03070
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 136 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3