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Protein

PI protein

Gene

pir

Organism
Escherichia coli
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Initiation for plasmid R6K DNA replication.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

DNA replication

Names & Taxonomyi

Protein namesi
Recommended name:
PI protein
Alternative name(s):
Replication initiation protein
Gene namesi
Name:pir
Encoded oniPlasmid R6K0 Publication
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 305305PI proteinPRO_0000068415Add
BLAST

Proteomic databases

PRIDEiP03067.

Interactioni

Subunit structurei

Homodimer.

Structurei

Secondary structure

1
305
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi14 – 185Combined sources
Helixi19 – 213Combined sources
Helixi22 – 276Combined sources
Helixi30 – 4112Combined sources
Beta strandi55 – 584Combined sources
Helixi59 – 668Combined sources
Helixi70 – 8617Combined sources
Helixi95 – 1028Combined sources
Beta strandi119 – 1279Combined sources
Turni128 – 1314Combined sources
Beta strandi132 – 1376Combined sources
Turni139 – 1413Combined sources
Helixi142 – 1443Combined sources
Helixi152 – 1543Combined sources
Beta strandi155 – 1595Combined sources
Helixi160 – 1656Combined sources
Helixi169 – 18012Combined sources
Beta strandi185 – 1873Combined sources
Beta strandi189 – 1946Combined sources
Helixi195 – 2028Combined sources
Beta strandi209 – 2124Combined sources
Beta strandi214 – 2174Combined sources
Helixi220 – 2267Combined sources
Helixi228 – 23811Combined sources
Beta strandi239 – 25012Combined sources
Beta strandi258 – 2658Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2NRAX-ray3.10C1-276[»]
ProteinModelPortaliP03067.
SMRiP03067. Positions 9-268.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP03067.

Family & Domainsi

Sequence similaritiesi

Belongs to the initiator RepB protein family.Curated

Family and domain databases

Gene3Di1.10.10.10. 2 hits.
InterProiIPR000525. Initiator_Rep_prot.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF01051. Rep_3. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 2 hits.

Sequencei

Sequence statusi: Complete.

P03067-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLKVMMDVN KKTKIRHRNE LNHTLAQLPL PAKRVMYMAL APIDSKEPLE
60 70 80 90 100
RGRVFKIRAE DLAALAKITP SLAYRQLKEG GKLLGASKIS LRGDDIIALA
110 120 130 140 150
KELNLPFTAK NSPEELDLNI IEWIAYSPDE GYLSLKFTRT IEPYISSLIG
160 170 180 190 200
KKNKFTTQLL TASLRLSSQY SSSLYQLIRK HYSNFKKKNY FIISVDELKE
210 220 230 240 250
ELIAYTFDKD GNIEYKYPDF PIFKRDVLNK AIAEIKKKTE ISFVGFTVHE
260 270 280 290 300
KEGRKISKLK FEFVVDEDEF SGDKDDEAFF MNLSEADAAF LKVFDETVPP

KKAKG
Length:305
Mass (Da):34,994
Last modified:January 1, 1990 - v2
Checksum:i9E463E3E2AEDAEA2
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti128 – 1281P → N (PubMed:6291046).Curated
Sequence conflicti246 – 2461F → L (PubMed:3000771).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti108 – 1081T → I in mutant COP41.
Natural varianti113 – 1131P → S in mutant COP50.
Natural varianti138 – 1381T → I in mutants TS22 and TRCOP21.
Natural varianti162 – 1621A → S in mutants COP21 and TRCOP21.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00768 Genomic DNA. Translation: CAA68737.1.
J01779 Genomic DNA. No translation available.
M11128 Genomic DNA. Translation: AAA26084.1.
PIRiS02261. IDECRK.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00768 Genomic DNA. Translation: CAA68737.1.
J01779 Genomic DNA. No translation available.
M11128 Genomic DNA. Translation: AAA26084.1.
PIRiS02261. IDECRK.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2NRAX-ray3.10C1-276[»]
ProteinModelPortaliP03067.
SMRiP03067. Positions 9-268.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP03067.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP03067.

Family and domain databases

Gene3Di1.10.10.10. 2 hits.
InterProiIPR000525. Initiator_Rep_prot.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF01051. Rep_3. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiPIR_ECOLX
AccessioniPrimary (citable) accession number: P03067
Secondary accession number(s): P10029
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 1, 1990
Last modified: May 11, 2016
This is version 71 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The expression of PI protein is autoregulated.
PI protein binds to direct repeated sequences within ori-gamma region for replication.

Keywords - Technical termi

3D-structure, Plasmid

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.