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Protein

PI protein

Gene

pir

Organism
Escherichia coli
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Initiation for plasmid R6K DNA replication.

Miscellaneous

The expression of PI protein is autoregulated.
PI protein binds to direct repeated sequences within ori-gamma region for replication.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processDNA replication

Names & Taxonomyi

Protein namesi
Recommended name:
PI protein
Alternative name(s):
Replication initiation protein
Gene namesi
Name:pir
Encoded oniPlasmid R6K0 Publication
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000684151 – 305PI proteinAdd BLAST305

Interactioni

Subunit structurei

Homodimer.

Structurei

Secondary structure

1305
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi14 – 18Combined sources5
Helixi19 – 21Combined sources3
Helixi22 – 27Combined sources6
Helixi30 – 41Combined sources12
Beta strandi55 – 58Combined sources4
Helixi59 – 66Combined sources8
Helixi70 – 86Combined sources17
Helixi95 – 102Combined sources8
Beta strandi119 – 127Combined sources9
Turni128 – 131Combined sources4
Beta strandi132 – 137Combined sources6
Turni139 – 141Combined sources3
Helixi142 – 144Combined sources3
Helixi152 – 154Combined sources3
Beta strandi155 – 159Combined sources5
Helixi160 – 165Combined sources6
Helixi169 – 180Combined sources12
Beta strandi185 – 187Combined sources3
Beta strandi189 – 194Combined sources6
Helixi195 – 202Combined sources8
Beta strandi209 – 212Combined sources4
Beta strandi214 – 217Combined sources4
Helixi220 – 226Combined sources7
Helixi228 – 238Combined sources11
Beta strandi239 – 250Combined sources12
Beta strandi258 – 265Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2NRAX-ray3.10C1-276[»]
ProteinModelPortaliP03067
SMRiP03067
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP03067

Family & Domainsi

Sequence similaritiesi

Belongs to the initiator RepB protein family.Curated

Family and domain databases

Gene3Di1.10.10.10, 2 hits
InterProiView protein in InterPro
IPR000525 Initiator_Rep_prot
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PfamiView protein in Pfam
PF01051 Rep_3, 1 hit
SUPFAMiSSF46785 SSF46785, 2 hits

Sequencei

Sequence statusi: Complete.

P03067-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLKVMMDVN KKTKIRHRNE LNHTLAQLPL PAKRVMYMAL APIDSKEPLE
60 70 80 90 100
RGRVFKIRAE DLAALAKITP SLAYRQLKEG GKLLGASKIS LRGDDIIALA
110 120 130 140 150
KELNLPFTAK NSPEELDLNI IEWIAYSPDE GYLSLKFTRT IEPYISSLIG
160 170 180 190 200
KKNKFTTQLL TASLRLSSQY SSSLYQLIRK HYSNFKKKNY FIISVDELKE
210 220 230 240 250
ELIAYTFDKD GNIEYKYPDF PIFKRDVLNK AIAEIKKKTE ISFVGFTVHE
260 270 280 290 300
KEGRKISKLK FEFVVDEDEF SGDKDDEAFF MNLSEADAAF LKVFDETVPP

KKAKG
Length:305
Mass (Da):34,994
Last modified:January 1, 1990 - v2
Checksum:i9E463E3E2AEDAEA2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti128P → N (PubMed:6291046).Curated1
Sequence conflicti246F → L (PubMed:3000771).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti108T → I in mutant COP41. 1
Natural varianti113P → S in mutant COP50. 1
Natural varianti138T → I in mutants TS22 and TRCOP21. 1
Natural varianti162A → S in mutants COP21 and TRCOP21. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00768 Genomic DNA Translation: CAA68737.1
J01779 Genomic DNA No translation available.
M11128 Genomic DNA Translation: AAA26084.1
PIRiS02261 IDECRK

Similar proteinsi

Entry informationi

Entry nameiPIR_ECOLX
AccessioniPrimary (citable) accession number: P03067
Secondary accession number(s): P10029
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 1, 1990
Last modified: May 23, 2018
This is version 75 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Plasmid

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