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Protein

TrfB transcriptional repressor protein

Gene

trfB

Organism
Escherichia coli
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

In conjunction with KorB, inhibits the transcription of kilA, trfA and korAB operons. In conjunction with KorC is responsible for the negative control of kilC and kilE operons.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi37 – 5620H-T-H motifSequence analysisAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
TrfB transcriptional repressor protein
Alternative name(s):
Regulatory protein KorA
Gene namesi
Name:trfB
Synonyms:korA
Encoded oniPlasmid IncP-alpha RK20 Publication
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 101101TrfB transcriptional repressor proteinPRO_0000068380Add
BLAST

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
korBP076742EBI-6405477,EBI-6405482

Protein-protein interaction databases

DIPiDIP-27644N.
IntActiP03052. 1 interaction.

Structurei

Secondary structure

1
101
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi7 – 148Combined sources
Helixi21 – 3111Combined sources
Helixi37 – 448Combined sources
Helixi48 – 6417Combined sources
Beta strandi71 – 788Combined sources
Helixi80 – 9415Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2W7NX-ray1.85A/B1-101[»]
5CKTX-ray2.00A/B/C1-99[»]
D1-101[»]
5CLVX-ray2.50A/B2-97[»]
E/F/I/J/M/N2-66[»]
5CM3X-ray2.30A/B1-97[»]
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP03052.

Family & Domainsi

Family and domain databases

InterProiIPR032428. TrfB.
[Graphical view]
PfamiPF16509. KORA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P03052-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKRLTESQF QEAIQGLEVG QQTIEIARGV LVDGKPQATF ATSLGLTRGA
60 70 80 90 100
VSQAVHRVWA AFEDKNLPEG YARVTAVLPE HQAYIVRKWE ADAKKKQETK

R
Length:101
Mass (Da):11,306
Last modified:July 21, 1986 - v1
Checksum:i25622FB8F98F21A5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03962 Genomic DNA. Translation: CAA27596.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03962 Genomic DNA. Translation: CAA27596.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2W7NX-ray1.85A/B1-101[»]
5CKTX-ray2.00A/B/C1-99[»]
D1-101[»]
5CLVX-ray2.50A/B2-97[»]
E/F/I/J/M/N2-66[»]
5CM3X-ray2.30A/B1-97[»]
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-27644N.
IntActiP03052. 1 interaction.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP03052.

Family and domain databases

InterProiIPR032428. TrfB.
[Graphical view]
PfamiPF16509. KORA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Map location and nucleotide sequence of korA, a key regulatory gene of promiscuous plasmid RK2."
    Bechhofer D.H., Figurski D.H.
    Nucleic Acids Res. 11:7453-7469(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The trfB region of broad host range plasmid RK2: the nucleotide sequence reveals incC and key regulatory gene trfB/korA/korD as overlapping genes."
    Thomas C.M., Smith C.A.
    Nucleic Acids Res. 14:4453-4469(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiKORA2_ECOLX
AccessioniPrimary (citable) accession number: P03052
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: June 8, 2016
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Plasmid

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.