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P03051

- ROP_ECOLX

UniProt

P03051 - ROP_ECOLX

Protein

Regulatory protein rop

Gene

rop

Organism
Escherichia coli
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Regulates plasmid DNA replication by modulating the initiation of transcription of the primer RNA precursor. Processing of the precursor of the primer, RNAII, is inhibited by hydrogen bonding of RNAII to its complementary sequence in RNAI. ROP increases the affinity of RNAI for RNAII and thus decreases the rate of replication initiation events.

    GO - Biological processi

    1. regulation of transcription, DNA-templated Source: UniProtKB-KW
    2. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Biological processi

    Transcription, Transcription regulation

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Regulatory protein rop
    Alternative name(s):
    RNA one modulator
    Short name:
    ROM
    Gene namesi
    Name:rop
    Encoded oniPlasmid ColE12 Publications
    Plasmid pMB11 Publication
    OrganismiEscherichia coli
    Taxonomic identifieri562 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 6363Regulatory protein ropPRO_0000068439Add
    BLAST

    Interactioni

    Subunit structurei

    Antiparallel homodimer.

    Protein-protein interaction databases

    DIPiDIP-48900N.

    Structurei

    Secondary structure

    1
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi3 – 2826
    Helixi32 – 5625
    Helixi58 – 636

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1B6QX-ray1.80A1-63[»]
    1F4MX-ray2.25A/B/C/D/E/F1-63[»]
    1F4NX-ray1.90A/B1-63[»]
    1GMGX-ray1.90A/B1-63[»]
    1GTOX-ray1.82A/B/C2-62[»]
    1NKDX-ray1.09A1-63[»]
    1QX8X-ray2.02A/B1-63[»]
    1ROPX-ray1.70A1-63[»]
    1RPOX-ray1.40A1-63[»]
    1RPRNMR-A/B1-63[»]
    1YO7X-ray2.80A/B1-63[»]
    2GHYX-ray2.50A/B1-63[»]
    2IJHX-ray1.80A/B/C1-63[»]
    2IJIX-ray2.30A1-63[»]
    2IJJX-ray1.90A/B/C1-63[»]
    2IJKX-ray1.55A/B1-63[»]
    3K79X-ray1.96A2-63[»]
    4DO2X-ray1.40A/B1-63[»]
    ProteinModelPortaliP03051.
    SMRiP03051. Positions 1-63.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP03051.

    Family & Domainsi

    Family and domain databases

    InterProiIPR000769. Regulatory_Rop.
    [Graphical view]
    PfamiPF01815. Rop. 1 hit.
    [Graphical view]
    PIRSFiPIRSF003229. Rop_reg. 1 hit.
    PRINTSiPR00835. ROPREGULATRY.
    ProDomiPD012167. Rop_reg. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SUPFAMiSSF47380. SSF47380. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P03051-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTKQEKTALN MARFIRSQTL TLLEKLNELD ADEQADICES LHDHADELYR   50
    SCLARFGDDG ENL 63
    Length:63
    Mass (Da):7,228
    Last modified:July 21, 1986 - v1
    Checksum:i0A1986BD29CF6FE4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J01749 Genomic DNA. Translation: AAB59736.1.
    J01564 Genomic DNA. Translation: AAA87380.1.
    PIRiA03587. RGECRE.
    RefSeqiNP_040367.1. NC_001371.1.
    NP_863575.1. NC_005019.1.
    YP_006940171.1. NC_018997.1.
    YP_006953570.1. NC_019076.1.
    YP_007316613.1. NC_019982.1.
    YP_794132.1. NC_008488.1.

    Genome annotation databases

    GeneIDi13877151.
    13906508.
    14401266.
    1446623.
    2693963.
    4397513.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J01749 Genomic DNA. Translation: AAB59736.1 .
    J01564 Genomic DNA. Translation: AAA87380.1 .
    PIRi A03587. RGECRE.
    RefSeqi NP_040367.1. NC_001371.1.
    NP_863575.1. NC_005019.1.
    YP_006940171.1. NC_018997.1.
    YP_006953570.1. NC_019076.1.
    YP_007316613.1. NC_019982.1.
    YP_794132.1. NC_008488.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1B6Q X-ray 1.80 A 1-63 [» ]
    1F4M X-ray 2.25 A/B/C/D/E/F 1-63 [» ]
    1F4N X-ray 1.90 A/B 1-63 [» ]
    1GMG X-ray 1.90 A/B 1-63 [» ]
    1GTO X-ray 1.82 A/B/C 2-62 [» ]
    1NKD X-ray 1.09 A 1-63 [» ]
    1QX8 X-ray 2.02 A/B 1-63 [» ]
    1ROP X-ray 1.70 A 1-63 [» ]
    1RPO X-ray 1.40 A 1-63 [» ]
    1RPR NMR - A/B 1-63 [» ]
    1YO7 X-ray 2.80 A/B 1-63 [» ]
    2GHY X-ray 2.50 A/B 1-63 [» ]
    2IJH X-ray 1.80 A/B/C 1-63 [» ]
    2IJI X-ray 2.30 A 1-63 [» ]
    2IJJ X-ray 1.90 A/B/C 1-63 [» ]
    2IJK X-ray 1.55 A/B 1-63 [» ]
    3K79 X-ray 1.96 A 2-63 [» ]
    4DO2 X-ray 1.40 A/B 1-63 [» ]
    ProteinModelPortali P03051.
    SMRi P03051. Positions 1-63.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-48900N.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 13877151.
    13906508.
    14401266.
    1446623.
    2693963.
    4397513.

    Miscellaneous databases

    EvolutionaryTracei P03051.

    Family and domain databases

    InterProi IPR000769. Regulatory_Rop.
    [Graphical view ]
    Pfami PF01815. Rop. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF003229. Rop_reg. 1 hit.
    PRINTSi PR00835. ROPREGULATRY.
    ProDomi PD012167. Rop_reg. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SUPFAMi SSF47380. SSF47380. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Control of ColE1 DNA replication: the rop gene product negatively affects transcription from the replication primer promoter."
      Cesareni G., Muesing M.A., Polisky B.
      Proc. Natl. Acad. Sci. U.S.A. 79:6313-6317(1982) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Plasmid: ColE1
    2. "Regulatory regions of ColE1 that are involved in determination of plasmid copy number."
      Som T., Tomizawa J.
      Proc. Natl. Acad. Sci. U.S.A. 80:3232-3236(1983) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Plasmid: ColE1
    3. "Complete nucleotide sequence of the Escherichia coli plasmid pBR322."
      Sutcliffe J.G.
      Cold Spring Harb. Symp. Quant. Biol. 43:77-90(1979) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Plasmid: pMB1
    4. "Proton nuclear magnetic resonance assignments and secondary structure determination of the ColE1 rop (rom) protein."
      Eberle W., Klaus W., Cesarini G., Sander C., Roesch P.
      Biochemistry 29:7402-7407(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR.
    5. "Structure of the ColE1 rop protein at 1.7-A resolution."
      Banner D.W., Kokkinidis M., Tsernoglou D.
      J. Mol. Biol. 196:657-675(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS).
    6. "Amino-acid substitutions in a surface turn modulate protein stability."
      Predki P.F., Agrawal V., Brunger A.T., Regan L.
      Nat. Struct. Biol. 3:54-58(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.82 ANGSTROMS).
    7. "Structural parameters for proteins derived from the atomic resolution (1.09-A) structure of a designed variant of the ColE1 ROP protein."
      Vlassi M., Dauter Z., Wilson K.S., Kokkinidis M.
      Acta Crystallogr. D 54:1245-1260(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.07 ANGSTROMS).
    8. Cited for: STRUCTURE BY NMR.

    Entry informationi

    Entry nameiROP_ECOLX
    AccessioniPrimary (citable) accession number: P03051
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: July 21, 1986
    Last modified: May 14, 2014
    This is version 89 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Plasmid

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references

    External Data

    Dasty 3