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P03051

- ROP_ECOLX

UniProt

P03051 - ROP_ECOLX

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Protein

Regulatory protein rop

Gene

rop

Organism
Escherichia coli
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli

Functioni

Regulates plasmid DNA replication by modulating the initiation of transcription of the primer RNA precursor. Processing of the precursor of the primer, RNAII, is inhibited by hydrogen bonding of RNAII to its complementary sequence in RNAI. ROP increases the affinity of RNAI for RNAII and thus decreases the rate of replication initiation events.

GO - Biological processi

  1. regulation of transcription, DNA-templated Source: UniProtKB-KW
  2. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Regulatory protein rop
Alternative name(s):
RNA one modulator
Short name:
ROM
Gene namesi
Name:rop
Encoded oniPlasmid ColE12 Publications
Plasmid pMB11 Publication
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 6363Regulatory protein ropPRO_0000068439Add
BLAST

Interactioni

Subunit structurei

Antiparallel homodimer.

Protein-protein interaction databases

DIPiDIP-48900N.

Structurei

Secondary structure

1
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi3 – 2826
Helixi32 – 5625
Helixi58 – 636

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1B6QX-ray1.80A1-63[»]
1F4MX-ray2.25A/B/C/D/E/F1-63[»]
1F4NX-ray1.90A/B1-63[»]
1GMGX-ray1.90A/B1-63[»]
1GTOX-ray1.82A/B/C2-62[»]
1NKDX-ray1.09A1-63[»]
1QX8X-ray2.02A/B1-63[»]
1ROPX-ray1.70A1-63[»]
1RPOX-ray1.40A1-63[»]
1RPRNMR-A/B1-63[»]
1YO7X-ray2.80A/B1-63[»]
2GHYX-ray2.50A/B1-63[»]
2IJHX-ray1.80A/B/C1-63[»]
2IJIX-ray2.30A1-63[»]
2IJJX-ray1.90A/B/C1-63[»]
2IJKX-ray1.55A/B1-63[»]
3K79X-ray1.96A2-63[»]
4DO2X-ray1.40A/B1-63[»]
ProteinModelPortaliP03051.
SMRiP03051. Positions 1-63.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP03051.

Family & Domainsi

Family and domain databases

InterProiIPR000769. Regulatory_Rop.
[Graphical view]
PfamiPF01815. Rop. 1 hit.
[Graphical view]
PIRSFiPIRSF003229. Rop_reg. 1 hit.
PRINTSiPR00835. ROPREGULATRY.
ProDomiPD012167. Rop_reg. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF47380. SSF47380. 1 hit.

Sequencei

Sequence statusi: Complete.

P03051-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTKQEKTALN MARFIRSQTL TLLEKLNELD ADEQADICES LHDHADELYR
60
SCLARFGDDG ENL
Length:63
Mass (Da):7,228
Last modified:July 21, 1986 - v1
Checksum:i0A1986BD29CF6FE4
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J01749 Genomic DNA. Translation: AAB59736.1.
J01564 Genomic DNA. Translation: AAA87380.1.
PIRiA03587. RGECRE.
RefSeqiNP_040367.1. NC_001371.1.
NP_863575.1. NC_005019.1.
YP_006940171.1. NC_018997.1.
YP_006953570.1. NC_019076.1.
YP_007316613.1. NC_019982.1.
YP_794132.1. NC_008488.1.

Genome annotation databases

GeneIDi13877151.
13906508.
14401266.
1446623.
2693963.
4397513.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J01749 Genomic DNA. Translation: AAB59736.1 .
J01564 Genomic DNA. Translation: AAA87380.1 .
PIRi A03587. RGECRE.
RefSeqi NP_040367.1. NC_001371.1.
NP_863575.1. NC_005019.1.
YP_006940171.1. NC_018997.1.
YP_006953570.1. NC_019076.1.
YP_007316613.1. NC_019982.1.
YP_794132.1. NC_008488.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1B6Q X-ray 1.80 A 1-63 [» ]
1F4M X-ray 2.25 A/B/C/D/E/F 1-63 [» ]
1F4N X-ray 1.90 A/B 1-63 [» ]
1GMG X-ray 1.90 A/B 1-63 [» ]
1GTO X-ray 1.82 A/B/C 2-62 [» ]
1NKD X-ray 1.09 A 1-63 [» ]
1QX8 X-ray 2.02 A/B 1-63 [» ]
1ROP X-ray 1.70 A 1-63 [» ]
1RPO X-ray 1.40 A 1-63 [» ]
1RPR NMR - A/B 1-63 [» ]
1YO7 X-ray 2.80 A/B 1-63 [» ]
2GHY X-ray 2.50 A/B 1-63 [» ]
2IJH X-ray 1.80 A/B/C 1-63 [» ]
2IJI X-ray 2.30 A 1-63 [» ]
2IJJ X-ray 1.90 A/B/C 1-63 [» ]
2IJK X-ray 1.55 A/B 1-63 [» ]
3K79 X-ray 1.96 A 2-63 [» ]
4DO2 X-ray 1.40 A/B 1-63 [» ]
ProteinModelPortali P03051.
SMRi P03051. Positions 1-63.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-48900N.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 13877151.
13906508.
14401266.
1446623.
2693963.
4397513.

Miscellaneous databases

EvolutionaryTracei P03051.

Family and domain databases

InterProi IPR000769. Regulatory_Rop.
[Graphical view ]
Pfami PF01815. Rop. 1 hit.
[Graphical view ]
PIRSFi PIRSF003229. Rop_reg. 1 hit.
PRINTSi PR00835. ROPREGULATRY.
ProDomi PD012167. Rop_reg. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
SUPFAMi SSF47380. SSF47380. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Control of ColE1 DNA replication: the rop gene product negatively affects transcription from the replication primer promoter."
    Cesareni G., Muesing M.A., Polisky B.
    Proc. Natl. Acad. Sci. U.S.A. 79:6313-6317(1982) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Plasmid: ColE1
  2. "Regulatory regions of ColE1 that are involved in determination of plasmid copy number."
    Som T., Tomizawa J.
    Proc. Natl. Acad. Sci. U.S.A. 80:3232-3236(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Plasmid: ColE1
  3. "Complete nucleotide sequence of the Escherichia coli plasmid pBR322."
    Sutcliffe J.G.
    Cold Spring Harb. Symp. Quant. Biol. 43:77-90(1979) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Plasmid: pMB1
  4. "Proton nuclear magnetic resonance assignments and secondary structure determination of the ColE1 rop (rom) protein."
    Eberle W., Klaus W., Cesarini G., Sander C., Roesch P.
    Biochemistry 29:7402-7407(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR.
  5. "Structure of the ColE1 rop protein at 1.7-A resolution."
    Banner D.W., Kokkinidis M., Tsernoglou D.
    J. Mol. Biol. 196:657-675(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS).
  6. "Amino-acid substitutions in a surface turn modulate protein stability."
    Predki P.F., Agrawal V., Brunger A.T., Regan L.
    Nat. Struct. Biol. 3:54-58(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.82 ANGSTROMS).
  7. "Structural parameters for proteins derived from the atomic resolution (1.09-A) structure of a designed variant of the ColE1 ROP protein."
    Vlassi M., Dauter Z., Wilson K.S., Kokkinidis M.
    Acta Crystallogr. D 54:1245-1260(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.07 ANGSTROMS).
  8. Cited for: STRUCTURE BY NMR.

Entry informationi

Entry nameiROP_ECOLX
AccessioniPrimary (citable) accession number: P03051
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: May 14, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Plasmid

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

External Data

Dasty 3