Reviewed, UniProtKB/Swiss-Prot P03045 (REGN_LAMBD)

Names and origin

Protein names

Recommended name:
    Antitermination protein N
Alternative name(s):
    Nucleocapsid protein
    Regulatory protein N
      Short name=PN

Gene names

Name:

N

Organism

Enterobacteria phage lambda (Bacteriophage lambda)

Taxonomic identifier

10710 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Caudovirales › Siphoviridae › Lambda-like viruses

Virus host

Escherichia coli [TaxID: 562]

Protein attributes

Sequence length	107 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function

Antitermination proteins positively regulate expression of the phage early and late gene operons. Bacterial host RNA polymerase modified by these antitermination proteins transcribes through termination sites that otherwise prevent expression of the regulated genes. N protein regulates the transition from the early to the middle stage of lytic development. It is a transcription antitermination protein that prevents termination at the rho-dependent tL and tR transcription termination sites.

Ontologies

Keywords
Biological process	Transcription Transcription regulation Transcription termination
Ligand	DNA-binding
Technical term	3D-structure
Gene Ontology (GO)
Biological process	regulation of transcription Inferred from electronic annotation. Source: UniProtKB-KW transcription termination Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 107

107

Antitermination protein N

PRO_0000077583

Secondary structure

1

.

.

.

.

.

.

.

.

107

Helix Strand Turn

Details...

Helix

4 – 10

7

Helix

12 – 20

9

Helix

23 – 25

3

Beta strand

26 – 29

4

Sequences

	Sequence		Length	Mass (Da)
	P03045-1 [UniParc]. Last modified January 1, 1990. Version 2. Checksum: E8144C2B0C47FE21	FASTA	107	12,255
	10 20 30 40 50 60 MDAQTRRRER RAEKQAQWKA ANPLLVGVSA KPVNLPILSL NRKPKSRVES ALNPIDLTVL 70 80 90 100 AEYHKQIESN LQRIERKNQR TWYSKPGERG ITCSGRQKIK GKSIPLI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Lambda N antitermination system: functional analysis of phage interactions with the host NusA protein." Schauer A.T., Carver D.L., Bigelow B., Baron L.S., Friedman D.I. J. Mol. Biol. 194:679-690(1987) [PubMed: 2821265] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Nucleotide sequence of bacteriophage lambda DNA." Sanger F., Coulson A.R., Hong G.F., Hill D.F., Petersen G.B. J. Mol. Biol. 162:729-773(1982) [PubMed: 6221115] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The DNA sequence of the phage lambda genome between PL and the gene bet." Ineichen K., Shepherd J.C.W., Bickle T.A. Nucleic Acids Res. 9:4639-4653(1981) [PubMed: 6458018] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"The N protein of bacteriophage lambda, defined by its DNA sequence, is highly basic." Franklin N.C., Bennett G.N. Gene 8:107-119(1979) [PubMed: 43815] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

J02459 Genomic DNA. Translation: AAA96578.1. Sequence problems.
M29653 Genomic DNA. Translation: AAA32249.1.

PIR

VNBPL. D94614.

RefSeq

NP_040625.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1QFQ	NMR	-	B	2-34	[»]

DisProt

DP00005.

ModBase

Search...

Genome annotation databases

GeneID

2703540.

GenomeReviews

Gene locus N in contig J02459_GR.

Family and domain databases

ProtoNet

Search...

Entry information

Entry name

REGN_LAMBD

Accession

Primary (citable) accession number: P03045
Secondary accession number(s): Q38647

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	January 1, 1990
Last modified:	September 22, 2009
This is version 55 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)