P03045 (REGN_LAMBD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Antitermination protein N

Alternative name(s):
Nucleocapsid protein
Regulatory protein N
Short name=PN

Gene names

Name:

N

Organism

Enterobacteria phage lambda (Bacteriophage lambda) [Reference proteome]

Taxonomic identifier

10710 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Caudovirales › Siphoviridae › Lambdalikevirus

Virus host

Escherichia coli [TaxID: 562]

Protein attributes

Sequence length	107 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function

Antitermination proteins positively regulate expression of the phage early and late gene operons. Bacterial host RNA polymerase modified by these antitermination proteins transcribes through termination sites that otherwise prevent expression of the regulated genes. N protein regulates the transition from the early to the middle stage of lytic development. It is a transcription antitermination protein that prevents termination at the rho-dependent tL and tR transcription termination sites.

Ontologies

Keywords
Biological process	Transcription Transcription regulation Transcription termination
Ligand	DNA-binding
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA-dependent transcription, termination Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 107

107

Antitermination protein N

PRO_0000077583

Experimental info

Sequence conflict

35

1

R → L in AAA32249. Ref.1

Secondary structure

1

.

.

.

.

.

.

.

.

107

Helix Strand Turn

Details...

Helix

4 – 10

7

Helix

12 – 20

9

Helix

23 – 25

3

Beta strand

26 – 29

4

Sequences

	Sequence		Length	Mass (Da)
	P03045 [UniParc]. Last modified February 8, 2011. Version 3. Checksum: F1EBAA32F3A1FE21	FASTA	107	12,298
	10 20 30 40 50 60 MDAQTRRRER RAEKQAQWKA ANPLLVGVSA KPVNRPILSL NRKPKSRVES ALNPIDLTVL 70 80 90 100 AEYHKQIESN LQRIERKNQR TWYSKPGERG ITCSGRQKIK GKSIPLI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Lambda N antitermination system: functional analysis of phage interactions with the host NusA protein." Schauer A.T., Carver D.L., Bigelow B., Baron L.S., Friedman D.I. J. Mol. Biol. 194:679-690(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Nucleotide sequence of bacteriophage lambda DNA." Sanger F., Coulson A.R., Hong G.F., Hill D.F., Petersen G.B. J. Mol. Biol. 162:729-773(1982) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The DNA sequence of the phage lambda genome between PL and the gene bet." Ineichen K., Shepherd J.C.W., Bickle T.A. Nucleic Acids Res. 9:4639-4653(1981) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"The N protein of bacteriophage lambda, defined by its DNA sequence, is highly basic." Franklin N.C., Bennett G.N. Gene 8:107-119(1979) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]	"Antitermination in bacteriophage lambda. The structure of the N36 peptide-boxB RNA complex." Scharpf M., Sticht H., Schweimer K., Boehm M., Hoffmann S., Rosch P. Eur. J. Biochem. 267:2397-2408(2000) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR OF 2-34.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

J02459 Genomic DNA. Translation: AAA96578.1.
M29653 Genomic DNA. Translation: AAA32249.1.

PIR

VNBPL. D94614.

RefSeq

NP_040625.1. NC_001416.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1QFQ	NMR	-	B	2-36	[»]

DisProt

DP00005.

ProteinModelPortal

P03045.

SMR

P03045. Positions 2-36.

ModBase

Search...

Protein-protein interaction databases

IntAct

P03045. 7 interactions.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

2703540.

Phylogenomic databases

ProtClustDB

CLSP2491390.

Family and domain databases

InterPro

IPR020952. Antiterm_prot_N_Arg-rich.
[Graphical view]

Pfam

PF11438. N36. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P03045.

Entry information

Entry name

REGN_LAMBD

Accession

Primary (citable) accession number: P03045
Secondary accession number(s): Q38647

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	February 8, 2011
Last modified:	May 1, 2013
This is version 76 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program