P03036 (RCRO_BP434) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Regulatory protein cro

Alternative name(s):
Antirepressor

Gene names

Name:

CRO

Organism

Bacteriophage 434

Taxonomic identifier

10712 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Caudovirales › Siphoviridae › Lambda-like viruses

Virus host

Escherichia coli [TaxID: 562]

Protein attributes

Sequence length	71 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Cro represses genes normally expressed in early phage development and is necessary for the late stage of lytic growth. It does this by binding to the OL and OR operators regions normally used by the repressor protein for lysogenic maintenance.
Sequence similarities	Contains 1 HTH cro/C1-type DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Developmental stage	Early protein
Ligand	DNA-binding
Molecular function	Repressor
Technical term	3D-structure
Gene Ontology (GO)
Biological process	transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 71

71

Regulatory protein cro

PRO_0000149707

Regions

Domain

8 – 61

54

HTH cro/C1-type

DNA binding

19 – 38

20

H-T-H motif

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

71

Helix Strand Turn

Details...

Helix

4 – 14

11

Helix

19 – 26

8

Helix

30 – 37

8

Helix

47 – 53

7

Helix

58 – 63

6

Sequences

	Sequence		Length	Mass (Da)
	P03036 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: 8783051BDF6D0606	FASTA	71	8,063
	10 20 30 40 50 60 MQTLSERLKK RRIALKMTQT ELATKAGVKQ QSIQLIEAGV TKRPRFLFEI AMALNCDPVW 70 LQYGTKRGKA A

References

[1]	"Nucleotide sequence of the cro-cII-oop region of bacteriophage 434 DNA." Grosschedl R., Schwarz E. Nucleic Acids Res. 6:867-881(1979) [PubMed: 375198] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Structure of phage 434 Cro protein at 2.35-A resolution." Mondragon A., Wolberger C., Harrison S.C. J. Mol. Biol. 205:179-188(1989) [PubMed: 2647998] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.35 ANGSTROMS).
[3]	"The phage 434 Cro/OR1 complex at 2.5-A resolution." Mondragon A., Harrison S.C. J. Mol. Biol. 219:321-334(1991) [PubMed: 2038059] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).
[4]	"Three-dimensional solution structure and stability of phage 434 Cro protein." Padmanabhan S., Jimenez M.A., Gonzalez C., Sanz J.M., Gimenez-Gallego G., Rico M. Biochemistry 36:6424-6436(1997) [PubMed: 9174359] [Abstract] Cited for: STRUCTURE BY NMR.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

V00635 Genomic DNA. Translation: CAA23908.1.
J02460 Genomic DNA. Translation: AAA32245.1.

PIR

RCBP4. A03572.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1ZUG	NMR	-	A	1-71	[»]
2CRO	X-ray	2.35	A	1-71	[»]
3CRO	X-ray	2.50	L/R	1-71	[»]

ProteinModelPortal

P03036.

SMR

P03036. Positions 1-71.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR001387. HTH_3.
IPR010982. Lambda_DNA-bd.
IPR000655. Phage_lambda_Cro.
[Graphical view]

Gene3D

G3DSA:1.10.260.40. G3DSA:1.10.260.40. 1 hit.

Pfam

PF01381. HTH_3. 1 hit.
[Graphical view]

PRINTS

PR00030. HTHCRO.

SMART

SM00530. HTH_XRE. 1 hit.
[Graphical view]

SUPFAM

SSF47413. Lambda_like_DNA. 1 hit.

PROSITE

PS50943. HTH_CROC1. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

RCRO_BP434

Accession

Primary (citable) accession number: P03036

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	December 14, 2011
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program