P03034 (RPC1_LAMBD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Repressor protein CI

Gene names

Name:

CI

Organism

Enterobacteria phage lambda (Bacteriophage lambda)

Taxonomic identifier

10710 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Caudovirales › Siphoviridae › Lambda-like viruses

Virus host

Escherichia coli [TaxID: 562]

Protein attributes

Sequence length	237 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Repressor protein CI allows phage lambda to reside inactively in the chromosome of its host bacterium. This lysogenic state is maintained by binding of regulatory protein CI to the OR and OL operators, preventing transcription of proteins necessary for lytic development.
Subunit structure	The active form is believed to be composed of 2 identical chains. The dimeric form binds more strongly to the lambda operator sites than does the monomer.
Miscellaneous	Bacterial cells harboring a lysogenic lambda phage are immune to further infection by lambda. The CI repressor protein inhibits the lytic development of any additional infecting phage particles. The region of the genome that codes for the CI repressor protein is known as the immunity region.
Sequence similarities	Contains 1 HTH cro/C1-type DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Ligand	DNA-binding
Molecular function	Repressor
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed; by host Ref.3

Chain

2 – 237

236

Repressor protein CI

PRO_0000149715

Regions

Domain

19 – 77

59

HTH cro/C1-type

DNA binding

30 – 49

20

H-T-H motif

Experimental info

Sequence conflict

67

1

A → T Ref.2

Sequence conflict

118

1

E → K Ref.2

Secondary structure

1

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.

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237

Helix Strand Turn

Details...

Helix

10 – 30

21

Helix

34 – 40

7

Helix

45 – 52

8

Helix

60 – 70

11

Helix

74 – 76

3

Helix

79 – 90

12

Turn

115 – 118

4

Beta strand

142 – 145

4

Beta strand

166 – 170

5

Beta strand

180 – 184

5

Beta strand

190 – 198

9

Beta strand

201 – 205

5

Beta strand

221 – 230

10

Helix

233 – 236

4

Sequences

	Sequence		Length	Mass (Da)
	P03034 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 4785A4915479ED97	FASTA	237	26,212
	10 20 30 40 50 60 MSTKKKPLTQ EQLEDARRLK AIYEKKKNEL GLSQESVADK MGMGQSGVGA LFNGINALNA 70 80 90 100 110 120 YNAALLAKIL KVSVEEFSPS IAREIYEMYE AVSMQPSLRS EYEYPVFSHV QAGMFSPELR 130 140 150 160 170 180 TFTKGDAERW VSTTKKASDS AFWLEVEGNS MTAPTGSKPS FPDGMLILVD PEQAVEPGDF 190 200 210 220 230 CIARLGGDEF TFKKLIRDSG QVFLQPLNPQ YPMIPCNESC SVVGKVIASQ WPEETFG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of bacteriophage lambda DNA." Sanger F., Coulson A.R., Hong G.F., Hill D.F., Petersen G.B. J. Mol. Biol. 162:729-773(1982) [PubMed: 6221115] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"DNA sequence of the bacteriophage gama cI gene." Sauer R.T. Nature 276:301-302(1978) [PubMed: 714163] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Primary structure of the lambda repressor." Sauer R.T., Anderegg R. Biochemistry 17:1092-1100(1978) [PubMed: 629949] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-237.
[4]	"Comparison of the structures of cro and lambda repressor proteins from bacteriophage lambda." Ohlendorf D.H., Anderson W.F., Lewis M., Pabo C.O., Matthews B.W. J. Mol. Biol. 169:757-769(1983) [PubMed: 6226802] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.2 ANGSTROMS) OF 1-93.
[5]	"Structure of the lambda complex at 2.5-A resolution: details of the repressor-operator interactions." Jordan S.R., Pabo C.O. Science 242:893-898(1988) [PubMed: 3187530] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 1-93.
[6]	Chattopadhyaya R., Ghosh K. Submitted (MAR-1999) to the PDB data bank Cited for: 3D-STRUCTURE MODELING.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

J02459 Genomic DNA. Translation: AAA96581.1.
X00166 Genomic DNA. Translation: CAA24991.1.

PIR

RPBPL. A14086.

RefSeq

NP_040628.1. NC_001416.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1F39	X-ray	1.90	A/B	137-236	[»]
1GFX	model	-	A/B/C/D	1-237	[»]
1J5G	model	-	A/B/C/D	1-237	[»]
1KCA	X-ray	2.91	A/B/C/D/E/F/G/H	137-236	[»]
1LLI	X-ray	2.10	A/B	2-92	[»]
1LMB	X-ray	1.80	3/4	2-92	[»]
1LRP	X-ray	3.20	A/B/C	2-93	[»]
1LWQ	model	-	A/B/C/D	1-237	[»]
1RIO	X-ray	2.30	A/B	1-91	[»]
3BDN	X-ray	3.91	A/B	2-237	[»]
3KZ3	X-ray	1.64	A/B	8-85	[»]

ProteinModelPortal

P03034.

SMR

P03034. Positions 2-237.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-17006N.

IntAct

P03034. 4 interactions.

Protein family/group databases

MEROPS

S24.002.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

2703538.

GenomeReviews

Gene locus CI in contig J02459_GR.

Phylogenomic databases

ProtClustDB

CLSP2509562.

Family and domain databases

InterPro

IPR001387. HTH_3.
IPR010982. Lambda_DNA-bd.
IPR019759. Peptidase_S24_S26.
IPR015927. Peptidase_S24_S26A/B/C.
IPR011056. Peptidase_S24_S26A/B/C_b-rbn.
[Graphical view]

Gene3D

G3DSA:1.10.260.40. G3DSA:1.10.260.40. 1 hit.
G3DSA:2.10.109.10. Pept_S24_S26_C. 1 hit.

Pfam

PF01381. HTH_3. 1 hit.
PF00717. Peptidase_S24. 1 hit.
[Graphical view]

SMART

SM00530. HTH_XRE. 1 hit.
[Graphical view]

SUPFAM

SSF47413. Lambda_like_DNA. 1 hit.
SSF51306. Pept_S24_S26_C. 1 hit.

PROSITE

PS50943. HTH_CROC1. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

RPC1_LAMBD

Accession

Primary (citable) accession number: P03034

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	January 23, 2007
Last modified:	December 14, 2011
This is version 104 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program