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P03018

- UVRD_ECOLI

UniProt

P03018 - UVRD_ECOLI

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Protein
DNA helicase II
Gene
uvrD, mutU, pdeB, rad, recL, b3813, JW3786
Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Has both ATPase and helicase activities. Unwinds DNA duplexes with 3' to 5' polarity with respect to the bound strand and initiates unwinding most effectively when a single-stranded region is present. Involved in the post-incision events of nucleotide excision repair and methyl-directed mismatch repair.

Catalytic activityi

ATP + H2O = ADP + phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei284 – 2841ATP By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi32 – 376ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ATP-dependent DNA helicase activity Source: InterPro
  3. DNA binding Source: UniProtKB-KW
  4. DNA helicase activity Source: CACAO
  5. protein binding Source: IntAct
Complete GO annotation...

GO - Biological processi

  1. DNA repair Source: UniProtKB-KW
  2. DNA unwinding involved in DNA replication Source: InterPro
  3. SOS response Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

DNA damage, DNA repair, DNA replication, SOS response

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG11064-MONOMER.
ECOL316407:JW3786-MONOMER.
MetaCyc:EG11064-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA helicase II (EC:3.6.4.12)
Gene namesi
Name:uvrD
Synonyms:mutU, pdeB, rad, recL
Ordered Locus Names:b3813, JW3786
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG11064. uvrD.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 720720DNA helicase II
PRO_0000102072Add
BLAST

Proteomic databases

PaxDbiP03018.
PRIDEiP03018.

Expressioni

Gene expression databases

GenevestigatoriP03018.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
lpdAP0A9P02EBI-559573,EBI-542856
metEP256652EBI-559573,EBI-551247
mutLP233677EBI-559573,EBI-554913

Protein-protein interaction databases

DIPiDIP-11103N.
IntActiP03018. 37 interactions.
MINTiMINT-1223204.
STRINGi511145.b3813.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi4 – 74
Beta strandi8 – 103
Helixi12 – 187
Beta strandi25 – 295
Beta strandi31 – 333
Helixi35 – 4814
Helixi54 – 563
Beta strandi57 – 637
Helixi64 – 7815
Beta strandi86 – 894
Helixi90 – 10011
Helixi103 – 1053
Beta strandi112 – 1143
Helixi116 – 12914
Turni134 – 1363
Helixi139 – 15113
Turni156 – 1583
Helixi166 – 18419
Beta strandi186 – 1883
Helixi189 – 20214
Helixi204 – 21310
Beta strandi216 – 2216
Helixi222 – 2243
Helixi227 – 23711
Turni238 – 2403
Beta strandi242 – 2476
Helixi249 – 2513
Helixi255 – 2573
Helixi263 – 2708
Beta strandi275 – 2795
Beta strandi283 – 2853
Helixi287 – 29711
Beta strandi300 – 3023
Beta strandi318 – 3258
Helixi326 – 34217
Helixi347 – 3493
Beta strandi350 – 3567
Helixi357 – 3593
Helixi360 – 36910
Beta strandi374 – 3796
Helixi382 – 3843
Helixi386 – 39914
Helixi404 – 4107
Helixi420 – 43213
Helixi437 – 44610
Helixi452 – 47120
Turni472 – 4743
Helixi477 – 48711
Helixi490 – 4956
Helixi500 – 51920
Helixi530 – 54011
Helixi542 – 5443
Beta strandi554 – 5585
Helixi560 – 5623
Beta strandi567 – 5726
Turni579 – 5813
Helixi583 – 5864
Beta strandi587 – 5904
Helixi592 – 60312
Beta strandi606 – 62015
Beta strandi623 – 6264
Helixi631 – 6355
Helixi638 – 6403
Beta strandi641 – 6433
Beta strandi646 – 6494

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2IS1X-ray2.90A/B1-680[»]
2IS2X-ray3.00A/B1-680[»]
2IS4X-ray2.60A/B1-680[»]
2IS6X-ray2.20A/B1-680[»]
3LFUX-ray1.80A1-647[»]
DisProtiDP00684.
ProteinModelPortaliP03018.
SMRiP03018. Positions 1-656.

Miscellaneous databases

EvolutionaryTraceiP03018.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini8 – 286279UvrD-like helicase ATP-binding
Add
BLAST
Domaini287 – 564278UvrD-like helicase C-terminal
Add
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0210.
HOGENOMiHOG000033016.
KOiK03657.
OMAiRLQIAFQ.
OrthoDBiEOG64N9TW.
PhylomeDBiP03018.

Family and domain databases

Gene3Di1.10.10.160. 1 hit.
3.40.50.300. 4 hits.
InterProiIPR013986. DExx_box_DNA_helicase_dom.
IPR005753. DNA_helicase_ATP-dep_UvrD.
IPR014017. DNA_helicase_UvrD-like_C.
IPR000212. DNA_helicase_UvrD/REP.
IPR027417. P-loop_NTPase.
IPR014016. UvrD-like_ATP-bd.
[Graphical view]
PANTHERiPTHR11070. PTHR11070. 1 hit.
PfamiPF00580. UvrD-helicase. 1 hit.
PF13361. UvrD_C. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01075. uvrD. 1 hit.
PROSITEiPS51198. UVRD_HELICASE_ATP_BIND. 1 hit.
PS51217. UVRD_HELICASE_CTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P03018-1 [UniParc]FASTAAdd to Basket

« Hide

MDVSYLLDSL NDKQREAVAA PRSNLLVLAG AGSGKTRVLV HRIAWLMSVE    50
NCSPYSIMAV TFTNKAAAEM RHRIGQLMGT SQGGMWVGTF HGLAHRLLRA 100
HHMDANLPQD FQILDSEDQL RLLKRLIKAM NLDEKQWPPR QAMWYINSQK 150
DEGLRPHHIQ SYGNPVEQTW QKVYQAYQEA CDRAGLVDFA ELLLRAHELW 200
LNKPHILQHY RERFTNILVD EFQDTNNIQY AWIRLLAGDT GKVMIVGDDD 250
QSIYGWRGAQ VENIQRFLND FPGAETIRLE QNYRSTSNIL SAANALIENN 300
NGRLGKKLWT DGADGEPISL YCAFNELDEA RFVVNRIKTW QDNGGALAEC 350
AILYRSNAQS RVLEEALLQA SMPYRIYGGM RFFERQEIKD ALSYLRLIAN 400
RNDDAAFERV VNTPTRGIGD RTLDVVRQTS RDRQLTLWQA CRELLQEKAL 450
AGRAASALQR FMELIDALAQ ETADMPLHVQ TDRVIKDSGL RTMYEQEKGE 500
KGQTRIENLE ELVTATRQFS YNEEDEDLMP LQAFLSHAAL EAGEGQADTW 550
QDAVQLMTLH SAKGLEFPQV FIVGMEEGMF PSQMSLDEGG RLEEERRLAY 600
VGVTRAMQKL TLTYAETRRL YGKEVYHRPS RFIGELPEEC VEEVRLRATV 650
SRPVSHQRMG TPMVENDSGY KLGQRVRHAK FGEGTIVNME GSGEHSRLQV 700
AFQGQGIKWL VAAYARLESV 720
Length:720
Mass (Da):81,990
Last modified:April 1, 1988 - v1
Checksum:iFA68E7267C77B49A
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti30 – 301G → D in uvrD252, which is UV sensitive.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti224 – 2241D → N in CAA25321. 1 Publication
Sequence conflicti291 – 2933SAA → NAR in CAA25321. 1 Publication
Sequence conflicti330 – 3301A → T in CAA25321. 1 Publication
Sequence conflicti540 – 5401L → V in CAA25321. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X00738 Genomic DNA. Translation: CAA25321.1.
D00069 Genomic DNA. Translation: BAA00048.1.
X04037 Genomic DNA. Translation: CAA27671.1.
M87049 Genomic DNA. Translation: AAA67609.1.
U00096 Genomic DNA. Translation: AAC76816.1.
AP009048 Genomic DNA. Translation: BAE77487.1.
X00225 Genomic DNA. Translation: CAA25043.1.
M38257 Genomic DNA. Translation: AAA24765.1.
PIRiF65185. HJECD2.
RefSeqiNP_418258.1. NC_000913.3.
YP_491628.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC76816; AAC76816; b3813.
BAE77487; BAE77487; BAE77487.
GeneIDi12934346.
948347.
KEGGiecj:Y75_p3364.
eco:b3813.
PATRICi32123127. VBIEscCol129921_3929.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X00738 Genomic DNA. Translation: CAA25321.1 .
D00069 Genomic DNA. Translation: BAA00048.1 .
X04037 Genomic DNA. Translation: CAA27671.1 .
M87049 Genomic DNA. Translation: AAA67609.1 .
U00096 Genomic DNA. Translation: AAC76816.1 .
AP009048 Genomic DNA. Translation: BAE77487.1 .
X00225 Genomic DNA. Translation: CAA25043.1 .
M38257 Genomic DNA. Translation: AAA24765.1 .
PIRi F65185. HJECD2.
RefSeqi NP_418258.1. NC_000913.3.
YP_491628.1. NC_007779.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2IS1 X-ray 2.90 A/B 1-680 [» ]
2IS2 X-ray 3.00 A/B 1-680 [» ]
2IS4 X-ray 2.60 A/B 1-680 [» ]
2IS6 X-ray 2.20 A/B 1-680 [» ]
3LFU X-ray 1.80 A 1-647 [» ]
DisProti DP00684.
ProteinModelPortali P03018.
SMRi P03018. Positions 1-656.
ModBasei Search...

Protein-protein interaction databases

DIPi DIP-11103N.
IntActi P03018. 37 interactions.
MINTi MINT-1223204.
STRINGi 511145.b3813.

Proteomic databases

PaxDbi P03018.
PRIDEi P03018.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC76816 ; AAC76816 ; b3813 .
BAE77487 ; BAE77487 ; BAE77487 .
GeneIDi 12934346.
948347.
KEGGi ecj:Y75_p3364.
eco:b3813.
PATRICi 32123127. VBIEscCol129921_3929.

Organism-specific databases

EchoBASEi EB1057.
EcoGenei EG11064. uvrD.

Phylogenomic databases

eggNOGi COG0210.
HOGENOMi HOG000033016.
KOi K03657.
OMAi RLQIAFQ.
OrthoDBi EOG64N9TW.
PhylomeDBi P03018.

Enzyme and pathway databases

BioCyci EcoCyc:EG11064-MONOMER.
ECOL316407:JW3786-MONOMER.
MetaCyc:EG11064-MONOMER.

Miscellaneous databases

EvolutionaryTracei P03018.
PROi P03018.

Gene expression databases

Genevestigatori P03018.

Family and domain databases

Gene3Di 1.10.10.160. 1 hit.
3.40.50.300. 4 hits.
InterProi IPR013986. DExx_box_DNA_helicase_dom.
IPR005753. DNA_helicase_ATP-dep_UvrD.
IPR014017. DNA_helicase_UvrD-like_C.
IPR000212. DNA_helicase_UvrD/REP.
IPR027417. P-loop_NTPase.
IPR014016. UvrD-like_ATP-bd.
[Graphical view ]
PANTHERi PTHR11070. PTHR11070. 1 hit.
Pfami PF00580. UvrD-helicase. 1 hit.
PF13361. UvrD_C. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
TIGRFAMsi TIGR01075. uvrD. 1 hit.
PROSITEi PS51198. UVRD_HELICASE_ATP_BIND. 1 hit.
PS51217. UVRD_HELICASE_CTER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of the uvrD gene of E. coli."
    Finch P.W., Emmerson P.T.
    Nucleic Acids Res. 12:5789-5799(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Determination of the initiation sites of transcription and translation of the uvrD gene of Escherichia coli."
    Yamamoto Y., Ogawa T., Shinagawa H., Nakayama T., Matsuo H., Ogawa H.
    J. Biochem. 99:1579-1590(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
  3. "Analysis of the Escherichia coli genome: DNA sequence of the region from 84.5 to 86.5 minutes."
    Daniels D.L., Plunkett G. III, Burland V.D., Blattner F.R.
    Science 257:771-778(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "Transcription of the uvrD gene of Escherichia coli is controlled by the lexA repressor and by attenuation."
    Easton A.M., Kushner S.R.
    Nucleic Acids Res. 11:8625-8640(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-258.
  7. "Recombination at ColE1 cer requires the Escherichia coli xerC gene product, a member of the lambda integrase family of site-specific recombinases."
    Colloms S.D., Sykora P., Szatmari G., Sherratt D.J.
    J. Bacteriol. 172:6973-6980(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-17.
    Strain: K12.
  8. "Characterization of DNA helicase II from a uvrD252 mutant of Escherichia coli."
    Washburn B.K., Kushner S.R.
    J. Bacteriol. 175:341-350(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: VARIANT UVRD252.

Entry informationi

Entry nameiUVRD_ECOLI
AccessioniPrimary (citable) accession number: P03018
Secondary accession number(s): P76758, Q2M8B9, Q47709
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: April 1, 1988
Last modified: June 11, 2014
This is version 141 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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