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P03018 (UVRD_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 139. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA helicase II

EC=3.6.4.12
Gene names
Name:uvrD
Synonyms:mutU, pdeB, rad, recL
Ordered Locus Names:b3813, JW3786
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length720 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Has both ATPase and helicase activities. Unwinds DNA duplexes with 3' to 5' polarity with respect to the bound strand and initiates unwinding most effectively when a single-stranded region is present. Involved in the post-incision events of nucleotide excision repair and methyl-directed mismatch repair.

Catalytic activity

ATP + H2O = ADP + phosphate.

Sequence similarities

Belongs to the helicase family. UvrD subfamily.

Contains 1 uvrD-like helicase ATP-binding domain.

Contains 1 uvrD-like helicase C-terminal domain.

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 720720DNA helicase II
PRO_0000102072

Regions

Domain8 – 286279UvrD-like helicase ATP-binding
Domain287 – 564278UvrD-like helicase C-terminal
Nucleotide binding32 – 376ATP By similarity

Sites

Binding site2841ATP By similarity

Natural variations

Natural variant301G → D in uvrD252, which is UV sensitive.

Experimental info

Sequence conflict2241D → N in CAA25321. Ref.1
Sequence conflict291 – 2933SAA → NAR in CAA25321. Ref.1
Sequence conflict3301A → T in CAA25321. Ref.1
Sequence conflict5401L → V in CAA25321. Ref.1

Secondary structure

..................................................................................................................... 720
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P03018 [UniParc].

Last modified April 1, 1988. Version 1.
Checksum: FA68E7267C77B49A

FASTA72081,990
        10         20         30         40         50         60 
MDVSYLLDSL NDKQREAVAA PRSNLLVLAG AGSGKTRVLV HRIAWLMSVE NCSPYSIMAV 

        70         80         90        100        110        120 
TFTNKAAAEM RHRIGQLMGT SQGGMWVGTF HGLAHRLLRA HHMDANLPQD FQILDSEDQL 

       130        140        150        160        170        180 
RLLKRLIKAM NLDEKQWPPR QAMWYINSQK DEGLRPHHIQ SYGNPVEQTW QKVYQAYQEA 

       190        200        210        220        230        240 
CDRAGLVDFA ELLLRAHELW LNKPHILQHY RERFTNILVD EFQDTNNIQY AWIRLLAGDT 

       250        260        270        280        290        300 
GKVMIVGDDD QSIYGWRGAQ VENIQRFLND FPGAETIRLE QNYRSTSNIL SAANALIENN 

       310        320        330        340        350        360 
NGRLGKKLWT DGADGEPISL YCAFNELDEA RFVVNRIKTW QDNGGALAEC AILYRSNAQS 

       370        380        390        400        410        420 
RVLEEALLQA SMPYRIYGGM RFFERQEIKD ALSYLRLIAN RNDDAAFERV VNTPTRGIGD 

       430        440        450        460        470        480 
RTLDVVRQTS RDRQLTLWQA CRELLQEKAL AGRAASALQR FMELIDALAQ ETADMPLHVQ 

       490        500        510        520        530        540 
TDRVIKDSGL RTMYEQEKGE KGQTRIENLE ELVTATRQFS YNEEDEDLMP LQAFLSHAAL 

       550        560        570        580        590        600 
EAGEGQADTW QDAVQLMTLH SAKGLEFPQV FIVGMEEGMF PSQMSLDEGG RLEEERRLAY 

       610        620        630        640        650        660 
VGVTRAMQKL TLTYAETRRL YGKEVYHRPS RFIGELPEEC VEEVRLRATV SRPVSHQRMG 

       670        680        690        700        710        720 
TPMVENDSGY KLGQRVRHAK FGEGTIVNME GSGEHSRLQV AFQGQGIKWL VAAYARLESV 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of the uvrD gene of E. coli."
Finch P.W., Emmerson P.T.
Nucleic Acids Res. 12:5789-5799(1984) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Determination of the initiation sites of transcription and translation of the uvrD gene of Escherichia coli."
Yamamoto Y., Ogawa T., Shinagawa H., Nakayama T., Matsuo H., Ogawa H.
J. Biochem. 99:1579-1590(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
[3]"Analysis of the Escherichia coli genome: DNA sequence of the region from 84.5 to 86.5 minutes."
Daniels D.L., Plunkett G. III, Burland V.D., Blattner F.R.
Science 257:771-778(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[5]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]"Transcription of the uvrD gene of Escherichia coli is controlled by the lexA repressor and by attenuation."
Easton A.M., Kushner S.R.
Nucleic Acids Res. 11:8625-8640(1983) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-258.
[7]"Recombination at ColE1 cer requires the Escherichia coli xerC gene product, a member of the lambda integrase family of site-specific recombinases."
Colloms S.D., Sykora P., Szatmari G., Sherratt D.J.
J. Bacteriol. 172:6973-6980(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-17.
Strain: K12.
[8]"Characterization of DNA helicase II from a uvrD252 mutant of Escherichia coli."
Washburn B.K., Kushner S.R.
J. Bacteriol. 175:341-350(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANT UVRD252.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X00738 Genomic DNA. Translation: CAA25321.1.
D00069 Genomic DNA. Translation: BAA00048.1.
X04037 Genomic DNA. Translation: CAA27671.1.
M87049 Genomic DNA. Translation: AAA67609.1.
U00096 Genomic DNA. Translation: AAC76816.1.
AP009048 Genomic DNA. Translation: BAE77487.1.
X00225 Genomic DNA. Translation: CAA25043.1.
M38257 Genomic DNA. Translation: AAA24765.1.
PIRHJECD2. F65185.
RefSeqNP_418258.1. NC_000913.3.
YP_491628.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2IS1X-ray2.90A/B1-680[»]
2IS2X-ray3.00A/B1-680[»]
2IS4X-ray2.60A/B1-680[»]
2IS6X-ray2.20A/B1-680[»]
3LFUX-ray1.80A1-647[»]
DisProtDP00684.
ProteinModelPortalP03018.
SMRP03018. Positions 1-656.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-11103N.
IntActP03018. 37 interactions.
MINTMINT-1223204.
STRING511145.b3813.

Proteomic databases

PaxDbP03018.
PRIDEP03018.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC76816; AAC76816; b3813.
BAE77487; BAE77487; BAE77487.
GeneID12934346.
948347.
KEGGecj:Y75_p3364.
eco:b3813.
PATRIC32123127. VBIEscCol129921_3929.

Organism-specific databases

EchoBASEEB1057.
EcoGeneEG11064. uvrD.

Phylogenomic databases

eggNOGCOG0210.
HOGENOMHOG000033016.
KOK03657.
OMAEDGVFPH.
OrthoDBEOG64N9TW.
PhylomeDBP03018.
ProtClustDBPRK11773.

Enzyme and pathway databases

BioCycEcoCyc:EG11064-MONOMER.
ECOL316407:JW3786-MONOMER.
MetaCyc:EG11064-MONOMER.

Gene expression databases

GenevestigatorP03018.

Family and domain databases

Gene3D1.10.10.160. 1 hit.
3.40.50.300. 4 hits.
InterProIPR013986. DExx_box_DNA_helicase_dom.
IPR005753. DNA_helicase_ATP-dep_UvrD.
IPR014017. DNA_helicase_UvrD-like_C.
IPR000212. DNA_helicase_UvrD/REP.
IPR027417. P-loop_NTPase.
IPR014016. UvrD-like_ATP-bd.
[Graphical view]
PANTHERPTHR11070. PTHR11070. 1 hit.
PfamPF00580. UvrD-helicase. 1 hit.
PF13361. UvrD_C. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR01075. uvrD. 1 hit.
PROSITEPS51198. UVRD_HELICASE_ATP_BIND. 1 hit.
PS51217. UVRD_HELICASE_CTER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP03018.
PROP03018.

Entry information

Entry nameUVRD_ECOLI
AccessionPrimary (citable) accession number: P03018
Secondary accession number(s): P76758, Q2M8B9, Q47709
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: April 1, 1988
Last modified: April 16, 2014
This is version 139 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene