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P03018

- UVRD_ECOLI

UniProt

P03018 - UVRD_ECOLI

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Protein

DNA helicase II

Gene

uvrD

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Has both ATPase and helicase activities. Unwinds DNA duplexes with 3' to 5' polarity with respect to the bound strand and initiates unwinding most effectively when a single-stranded region is present. Involved in the post-incision events of nucleotide excision repair and methyl-directed mismatch repair.

Catalytic activityi

ATP + H2O = ADP + phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei284 – 2841ATPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi32 – 376ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA binding Source: UniProtKB-KW
  3. DNA helicase activity Source: CACAO
  4. DNA translocase activity Source: EcoCyc
  5. single-stranded DNA-dependent ATP-dependent DNA helicase activity Source: EcoCyc

GO - Biological processi

  1. ATP catabolic process Source: GOC
  2. DNA duplex unwinding Source: EcoCyc
  3. DNA unwinding involved in DNA replication Source: InterPro
  4. mismatch repair Source: EcoCyc
  5. nucleotide-excision repair Source: EcoCyc
  6. protein homooligomerization Source: EcoCyc
  7. replication fork processing Source: EcoCyc
  8. rolling circle DNA replication Source: EcoCyc
  9. SOS response Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

DNA damage, DNA repair, DNA replication, SOS response

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG11064-MONOMER.
ECOL316407:JW3786-MONOMER.
MetaCyc:EG11064-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA helicase II (EC:3.6.4.12)
Gene namesi
Name:uvrD
Synonyms:mutU, pdeB, rad, recL
Ordered Locus Names:b3813, JW3786
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG11064. uvrD.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: EcoCyc
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 720720DNA helicase IIPRO_0000102072Add
BLAST

Proteomic databases

PaxDbiP03018.
PRIDEiP03018.

Expressioni

Gene expression databases

GenevestigatoriP03018.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
lpdAP0A9P02EBI-559573,EBI-542856
metEP256652EBI-559573,EBI-551247
mutLP233677EBI-559573,EBI-554913

Protein-protein interaction databases

DIPiDIP-11103N.
IntActiP03018. 37 interactions.
MINTiMINT-1223204.
STRINGi511145.b3813.

Structurei

Secondary structure

1
720
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi4 – 74Combined sources
Beta strandi8 – 103Combined sources
Helixi12 – 187Combined sources
Beta strandi25 – 295Combined sources
Beta strandi31 – 333Combined sources
Helixi35 – 4814Combined sources
Helixi54 – 563Combined sources
Beta strandi57 – 637Combined sources
Helixi64 – 7815Combined sources
Beta strandi86 – 894Combined sources
Helixi90 – 10011Combined sources
Helixi103 – 1053Combined sources
Beta strandi112 – 1143Combined sources
Helixi116 – 12914Combined sources
Turni134 – 1363Combined sources
Helixi139 – 15113Combined sources
Turni156 – 1583Combined sources
Helixi166 – 18419Combined sources
Beta strandi186 – 1883Combined sources
Helixi189 – 20214Combined sources
Helixi204 – 21310Combined sources
Beta strandi216 – 2216Combined sources
Helixi222 – 2243Combined sources
Helixi227 – 23711Combined sources
Turni238 – 2403Combined sources
Beta strandi242 – 2476Combined sources
Helixi249 – 2513Combined sources
Helixi255 – 2573Combined sources
Helixi263 – 2708Combined sources
Beta strandi275 – 2795Combined sources
Beta strandi283 – 2853Combined sources
Helixi287 – 29711Combined sources
Beta strandi300 – 3023Combined sources
Beta strandi318 – 3258Combined sources
Helixi326 – 34217Combined sources
Helixi347 – 3493Combined sources
Beta strandi350 – 3567Combined sources
Helixi357 – 3593Combined sources
Helixi360 – 36910Combined sources
Beta strandi374 – 3796Combined sources
Helixi382 – 3843Combined sources
Helixi386 – 39914Combined sources
Helixi404 – 4107Combined sources
Helixi420 – 43213Combined sources
Helixi437 – 44610Combined sources
Helixi452 – 47120Combined sources
Turni472 – 4743Combined sources
Helixi477 – 48711Combined sources
Helixi490 – 4956Combined sources
Helixi500 – 51920Combined sources
Helixi530 – 54011Combined sources
Helixi542 – 5443Combined sources
Beta strandi554 – 5585Combined sources
Helixi560 – 5623Combined sources
Beta strandi567 – 5726Combined sources
Turni579 – 5813Combined sources
Helixi583 – 5864Combined sources
Beta strandi587 – 5904Combined sources
Helixi592 – 60312Combined sources
Beta strandi606 – 62015Combined sources
Beta strandi623 – 6264Combined sources
Helixi631 – 6355Combined sources
Helixi638 – 6403Combined sources
Beta strandi641 – 6433Combined sources
Beta strandi646 – 6494Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2IS1X-ray2.90A/B1-680[»]
2IS2X-ray3.00A/B1-680[»]
2IS4X-ray2.60A/B1-680[»]
2IS6X-ray2.20A/B1-680[»]
3LFUX-ray1.80A1-647[»]
DisProtiDP00684.
ProteinModelPortaliP03018.
SMRiP03018. Positions 1-656.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP03018.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini8 – 286279UvrD-like helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini287 – 564278UvrD-like helicase C-terminalPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the helicase family. UvrD subfamily.Curated
Contains 1 uvrD-like helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 uvrD-like helicase C-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0210.
HOGENOMiHOG000033016.
InParanoidiP03018.
KOiK03657.
OMAiRLQIAFQ.
OrthoDBiEOG64N9TW.
PhylomeDBiP03018.

Family and domain databases

Gene3Di1.10.10.160. 1 hit.
3.40.50.300. 4 hits.
InterProiIPR013986. DExx_box_DNA_helicase_dom.
IPR005753. DNA_helicase_ATP-dep_UvrD.
IPR014017. DNA_helicase_UvrD-like_C.
IPR000212. DNA_helicase_UvrD/REP.
IPR027417. P-loop_NTPase.
IPR014016. UvrD-like_ATP-bd.
[Graphical view]
PANTHERiPTHR11070. PTHR11070. 1 hit.
PfamiPF00580. UvrD-helicase. 1 hit.
PF13361. UvrD_C. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01075. uvrD. 1 hit.
PROSITEiPS51198. UVRD_HELICASE_ATP_BIND. 1 hit.
PS51217. UVRD_HELICASE_CTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P03018-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDVSYLLDSL NDKQREAVAA PRSNLLVLAG AGSGKTRVLV HRIAWLMSVE
60 70 80 90 100
NCSPYSIMAV TFTNKAAAEM RHRIGQLMGT SQGGMWVGTF HGLAHRLLRA
110 120 130 140 150
HHMDANLPQD FQILDSEDQL RLLKRLIKAM NLDEKQWPPR QAMWYINSQK
160 170 180 190 200
DEGLRPHHIQ SYGNPVEQTW QKVYQAYQEA CDRAGLVDFA ELLLRAHELW
210 220 230 240 250
LNKPHILQHY RERFTNILVD EFQDTNNIQY AWIRLLAGDT GKVMIVGDDD
260 270 280 290 300
QSIYGWRGAQ VENIQRFLND FPGAETIRLE QNYRSTSNIL SAANALIENN
310 320 330 340 350
NGRLGKKLWT DGADGEPISL YCAFNELDEA RFVVNRIKTW QDNGGALAEC
360 370 380 390 400
AILYRSNAQS RVLEEALLQA SMPYRIYGGM RFFERQEIKD ALSYLRLIAN
410 420 430 440 450
RNDDAAFERV VNTPTRGIGD RTLDVVRQTS RDRQLTLWQA CRELLQEKAL
460 470 480 490 500
AGRAASALQR FMELIDALAQ ETADMPLHVQ TDRVIKDSGL RTMYEQEKGE
510 520 530 540 550
KGQTRIENLE ELVTATRQFS YNEEDEDLMP LQAFLSHAAL EAGEGQADTW
560 570 580 590 600
QDAVQLMTLH SAKGLEFPQV FIVGMEEGMF PSQMSLDEGG RLEEERRLAY
610 620 630 640 650
VGVTRAMQKL TLTYAETRRL YGKEVYHRPS RFIGELPEEC VEEVRLRATV
660 670 680 690 700
SRPVSHQRMG TPMVENDSGY KLGQRVRHAK FGEGTIVNME GSGEHSRLQV
710 720
AFQGQGIKWL VAAYARLESV
Length:720
Mass (Da):81,990
Last modified:April 1, 1988 - v1
Checksum:iFA68E7267C77B49A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti224 – 2241D → N in CAA25321. (PubMed:6379604)Curated
Sequence conflicti291 – 2933SAA → NAR in CAA25321. (PubMed:6379604)Curated
Sequence conflicti330 – 3301A → T in CAA25321. (PubMed:6379604)Curated
Sequence conflicti540 – 5401L → V in CAA25321. (PubMed:6379604)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti30 – 301G → D in uvrD252, which is UV sensitive.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X00738 Genomic DNA. Translation: CAA25321.1.
D00069 Genomic DNA. Translation: BAA00048.1.
X04037 Genomic DNA. Translation: CAA27671.1.
M87049 Genomic DNA. Translation: AAA67609.1.
U00096 Genomic DNA. Translation: AAC76816.1.
AP009048 Genomic DNA. Translation: BAE77487.1.
X00225 Genomic DNA. Translation: CAA25043.1.
M38257 Genomic DNA. Translation: AAA24765.1.
PIRiF65185. HJECD2.
RefSeqiNP_418258.1. NC_000913.3.
YP_491628.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC76816; AAC76816; b3813.
BAE77487; BAE77487; BAE77487.
GeneIDi12934346.
948347.
KEGGiecj:Y75_p3364.
eco:b3813.
PATRICi32123127. VBIEscCol129921_3929.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X00738 Genomic DNA. Translation: CAA25321.1 .
D00069 Genomic DNA. Translation: BAA00048.1 .
X04037 Genomic DNA. Translation: CAA27671.1 .
M87049 Genomic DNA. Translation: AAA67609.1 .
U00096 Genomic DNA. Translation: AAC76816.1 .
AP009048 Genomic DNA. Translation: BAE77487.1 .
X00225 Genomic DNA. Translation: CAA25043.1 .
M38257 Genomic DNA. Translation: AAA24765.1 .
PIRi F65185. HJECD2.
RefSeqi NP_418258.1. NC_000913.3.
YP_491628.1. NC_007779.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2IS1 X-ray 2.90 A/B 1-680 [» ]
2IS2 X-ray 3.00 A/B 1-680 [» ]
2IS4 X-ray 2.60 A/B 1-680 [» ]
2IS6 X-ray 2.20 A/B 1-680 [» ]
3LFU X-ray 1.80 A 1-647 [» ]
DisProti DP00684.
ProteinModelPortali P03018.
SMRi P03018. Positions 1-656.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-11103N.
IntActi P03018. 37 interactions.
MINTi MINT-1223204.
STRINGi 511145.b3813.

Proteomic databases

PaxDbi P03018.
PRIDEi P03018.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC76816 ; AAC76816 ; b3813 .
BAE77487 ; BAE77487 ; BAE77487 .
GeneIDi 12934346.
948347.
KEGGi ecj:Y75_p3364.
eco:b3813.
PATRICi 32123127. VBIEscCol129921_3929.

Organism-specific databases

EchoBASEi EB1057.
EcoGenei EG11064. uvrD.

Phylogenomic databases

eggNOGi COG0210.
HOGENOMi HOG000033016.
InParanoidi P03018.
KOi K03657.
OMAi RLQIAFQ.
OrthoDBi EOG64N9TW.
PhylomeDBi P03018.

Enzyme and pathway databases

BioCyci EcoCyc:EG11064-MONOMER.
ECOL316407:JW3786-MONOMER.
MetaCyc:EG11064-MONOMER.

Miscellaneous databases

EvolutionaryTracei P03018.
PROi P03018.

Gene expression databases

Genevestigatori P03018.

Family and domain databases

Gene3Di 1.10.10.160. 1 hit.
3.40.50.300. 4 hits.
InterProi IPR013986. DExx_box_DNA_helicase_dom.
IPR005753. DNA_helicase_ATP-dep_UvrD.
IPR014017. DNA_helicase_UvrD-like_C.
IPR000212. DNA_helicase_UvrD/REP.
IPR027417. P-loop_NTPase.
IPR014016. UvrD-like_ATP-bd.
[Graphical view ]
PANTHERi PTHR11070. PTHR11070. 1 hit.
Pfami PF00580. UvrD-helicase. 1 hit.
PF13361. UvrD_C. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
TIGRFAMsi TIGR01075. uvrD. 1 hit.
PROSITEi PS51198. UVRD_HELICASE_ATP_BIND. 1 hit.
PS51217. UVRD_HELICASE_CTER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The nucleotide sequence of the uvrD gene of E. coli."
    Finch P.W., Emmerson P.T.
    Nucleic Acids Res. 12:5789-5799(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Determination of the initiation sites of transcription and translation of the uvrD gene of Escherichia coli."
    Yamamoto Y., Ogawa T., Shinagawa H., Nakayama T., Matsuo H., Ogawa H.
    J. Biochem. 99:1579-1590(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
  3. "Analysis of the Escherichia coli genome: DNA sequence of the region from 84.5 to 86.5 minutes."
    Daniels D.L., Plunkett G. III, Burland V.D., Blattner F.R.
    Science 257:771-778(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "Transcription of the uvrD gene of Escherichia coli is controlled by the lexA repressor and by attenuation."
    Easton A.M., Kushner S.R.
    Nucleic Acids Res. 11:8625-8640(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-258.
  7. "Recombination at ColE1 cer requires the Escherichia coli xerC gene product, a member of the lambda integrase family of site-specific recombinases."
    Colloms S.D., Sykora P., Szatmari G., Sherratt D.J.
    J. Bacteriol. 172:6973-6980(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-17.
    Strain: K12.
  8. "Characterization of DNA helicase II from a uvrD252 mutant of Escherichia coli."
    Washburn B.K., Kushner S.R.
    J. Bacteriol. 175:341-350(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: VARIANT UVRD252.

Entry informationi

Entry nameiUVRD_ECOLI
AccessioniPrimary (citable) accession number: P03018
Secondary accession number(s): P76758, Q2M8B9, Q47709
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: April 1, 1988
Last modified: November 26, 2014
This is version 144 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3