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Protein

Serine recombinase gin

Gene

gin

Organism
Enterobacteria phage Mu (Bacteriophage Mu)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Performs inversion of a viral 3 kp segment (G-segment) that encodes two alternate pairs of tail fiber proteins thereby modifying the host specificity of the virus. Binds as a dimer to the viral gix sites which are 34-bp palindromic sequences that flank the invertible G-segment. Catalyzes site-specific recombination in the presence of the host factor Fis. Gin dimers bound to each of the gix sites and host factor Fis bound to the enhancer come together to form the synaptic complex. Each Gin monomer introduces a nick and becomes covalently attached to the 5'-phosphate of the DNA, resulting in double-stranded staggered breaks at both recombination sites. A 180 degrees rotation of one of the two Gin dimers followed by religation of the DNA leads to the inversion of the G-segment (G+ or G- orientation).4 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei9O-(5'-phospho-DNA)-serine intermediatePROSITE-ProRule annotation1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi138 – 183H-T-H motifAdd BLAST46

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

DNA invertase, Hydrolase, Ligase

Keywords - Biological processi

DNA integration, DNA recombination, Viral receptor tropism switching, Virus entry into host cell

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine recombinase gin (EC:3.1.22.-, EC:6.5.1.-)
Alternative name(s):
G-segment invertase
Short name:
Gin
Gene product 53
Short name:
gp53
Gene namesi
Name:gin
Ordered Locus Names:Mup53
OrganismiEnterobacteria phage Mu (Bacteriophage Mu)
Taxonomic identifieri10677 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesMyoviridaeMulikevirus
Virus hostiEnterobacteriaceae [TaxID: 543]
Proteomesi
  • UP000002611 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi9S → A, L or T: Recombination deficient. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001963541 – 193Serine recombinase ginAdd BLAST193

Expressioni

Inductioni

Expressed in the late phase of the viral replicative cycle. Expression of late genes is activated by the viral late transcription activator C.1 Publication

Keywords - Developmental stagei

Late protein

Interactioni

Subunit structurei

Homodimer. During inversion, two dimers associate to form a homotetramer.2 Publications

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3UJ3X-ray3.80X1-193[»]
4M6FX-ray4.99A1-193[»]
ProteinModelPortaliP03015.
SMRiP03015.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 134Resolvase/invertase-type recombinase catalyticPROSITE-ProRule annotationAdd BLAST134

Domaini

The dimerization region is in the N-terminus.

Sequence similaritiesi

Contains 1 resolvase/invertase-type recombinase catalytic domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
3.40.50.1390. 1 hit.
InterProiIPR009057. Homeodomain-like.
IPR006118. Recombinase_CS.
IPR006119. Resolv_N.
IPR006120. Resolvase_HTH_dom.
[Graphical view]
PfamiPF02796. HTH_7. 1 hit.
PF00239. Resolvase. 1 hit.
[Graphical view]
SMARTiSM00857. Resolvase. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF53041. SSF53041. 1 hit.
PROSITEiPS00397. RECOMBINASES_1. 1 hit.
PS00398. RECOMBINASES_2. 1 hit.
PS51736. RECOMBINASES_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P03015-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLIGYVRVST NDQNTDLQRN ALVCAGCEQI FEDKLSGTRT DRPGLKRALK
60 70 80 90 100
RLQKGDTLVV WKLDRLGRSM KHLISLVGEL RERGINFRSL TDSIDTSSPM
110 120 130 140 150
GRFFFHVMGA LAEMERELII ERTMAGLAAA RNKGRIGGRP PKLTKAEWEQ
160 170 180 190
AGRLLAQGIP RKQVALIYDV ALSTLYKKHP AKRAHIENDD RIN
Length:193
Mass (Da):21,766
Last modified:December 1, 2000 - v2
Checksum:i86ED5D1C6ADD17DB
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti48A → D no nucleotide entry (PubMed:6310572).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF083977 Genomic DNA. Translation: AAF01129.1.
V01463 Genomic DNA. Translation: CAA24708.1.
PIRiS02705. JWBPU.
RefSeqiNP_050655.1. NC_000929.1.

Genome annotation databases

GeneIDi2636258.
KEGGivg:2636258.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF083977 Genomic DNA. Translation: AAF01129.1.
V01463 Genomic DNA. Translation: CAA24708.1.
PIRiS02705. JWBPU.
RefSeqiNP_050655.1. NC_000929.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3UJ3X-ray3.80X1-193[»]
4M6FX-ray4.99A1-193[»]
ProteinModelPortaliP03015.
SMRiP03015.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi2636258.
KEGGivg:2636258.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
3.40.50.1390. 1 hit.
InterProiIPR009057. Homeodomain-like.
IPR006118. Recombinase_CS.
IPR006119. Resolv_N.
IPR006120. Resolvase_HTH_dom.
[Graphical view]
PfamiPF02796. HTH_7. 1 hit.
PF00239. Resolvase. 1 hit.
[Graphical view]
SMARTiSM00857. Resolvase. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF53041. SSF53041. 1 hit.
PROSITEiPS00397. RECOMBINASES_1. 1 hit.
PS00398. RECOMBINASES_2. 1 hit.
PS51736. RECOMBINASES_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGIN_BPMU
AccessioniPrimary (citable) accession number: P03015
Secondary accession number(s): Q9T1U8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: December 1, 2000
Last modified: November 2, 2016
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

The orientation of the G segment is defined as G+ and G-. G+ orientation provides S-U fibers whereas G- provides S'-U' fibers. S-U and S'-U' dont have the same host range (e.g. respectively E.coli and C.freundii).

Caution

Translation initiates from a non-canonical start codon (GUG).Curated

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.