P03015 (DNIV_BPMU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA-invertase

Gene names

Name:	gin
Synonyms:	51

Organism

Enterobacteria phage Mu (Bacteriophage Mu) [Reference proteome]

Taxonomic identifier

10677 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Caudovirales › Myoviridae › Mulikevirus

Virus host

Enterobacteriaceae [TaxID: 543]

Protein attributes

Sequence length	193 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This protein catalyzes the inversion of a 3000-bp segment of phage DNA. The inversion results in a modification of the 3'-end of the tail fiber gene and alters the host specificity. Ref.5
Sequence similarities	Belongs to the site-specific recombinase resolvase family.

Ontologies

Keywords
Biological process	DNA integration DNA recombination
Ligand	DNA-binding
Molecular function	DNA invertase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA integration Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW recombinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 193	193	DNA-invertase		PRO_0000196354
Regions
	DNA binding	161 – 180	20	H-T-H motif Potential
Sites
	Active site	9	1	O-(5'-phospho-DNA)-serine intermediate
Experimental info
	Sequence conflict	48	1	A → D Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P03015 [UniParc]. Last modified December 1, 2000. Version 2. Checksum: 86ED5D1C6ADD17DB	FASTA	193	21,766
	10 20 30 40 50 60 MLIGYVRVST NDQNTDLQRN ALVCAGCEQI FEDKLSGTRT DRPGLKRALK RLQKGDTLVV 70 80 90 100 110 120 WKLDRLGRSM KHLISLVGEL RERGINFRSL TDSIDTSSPM GRFFFHVMGA LAEMERELII 130 140 150 160 170 180 ERTMAGLAAA RNKGRIGGRP PKLTKAEWEQ AGRLLAQGIP RKQVALIYDV ALSTLYKKHP 190 AKRAHIENDD RIN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"DNA inversions in the chromosome of Escherichia coli and in bacteriophage Mu: relationship to other site-specific recombination systems." Plasterk R.H.A., Brinkman A., van de Putte P. Proc. Natl. Acad. Sci. U.S.A. 80:5355-5358(1983) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Bacteriophage Mu genome sequence: analysis and comparison with Mu-like prophages in Haemophilus, Neisseria and Deinococcus." Morgan G.J., Hatfull G.F., Casjens S., Hendrix R.W. J. Mol. Biol. 317:337-359(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"Methylation regulates the expression of a DNA-modification function encoded by bacteriophage Mu." Kahmann R. Cold Spring Harb. Symp. Quant. Biol. 47:639-646(1983) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 173-193.
[4]	"The DNA invertase Gin of phage Mu: formation of a covalent complex with DNA via a phosphoserine at amino acid position 9." Klippel A., Mertens G., Patschinsky T., Kahmann R. EMBO J. 7:1229-1237(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-16, COVALENT COMPLEX WITH DNA.
[5]	"Isolation and characterization of unusual gin mutants." Klippel A., Cloppenborg K., Kahmann R. EMBO J. 7:3983-3989(1988) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION OF MUTANTS.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF083977 Genomic DNA. Translation: AAF01129.1.
V01463 Genomic DNA. Translation: CAA24708.1.

PIR

JWBPU. S02705.

RefSeq

NP_050655.1. NC_000929.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3UJ3	X-ray	3.80	X	1-193	[»]

ProteinModelPortal

P03015.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

2636258.

Phylogenomic databases

ProtClustDB

CLSP2343583.

Family and domain databases

Gene3D

1.10.10.60. 1 hit.
3.40.50.1390. 1 hit.

InterPro

IPR009057. Homeodomain-like.
IPR006118. Recombinase_CS.
IPR006119. Resolv_N.
IPR006120. Resolvase_HTH_dom.
[Graphical view]

Pfam

PF02796. HTH_7. 1 hit.
PF00239. Resolvase. 1 hit.
[Graphical view]

SMART

SM00857. Resolvase. 1 hit.
[Graphical view]

SUPFAM

SSF46689. Homeodomain_like. 1 hit.
SSF53041. Resolv_N. 1 hit.

PROSITE

PS00397. RECOMBINASES_1. 1 hit.
PS00398. RECOMBINASES_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

DNIV_BPMU

Accession

Primary (citable) accession number: P03015
Secondary accession number(s): Q9T1U8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	December 1, 2000
Last modified:	May 1, 2013
This is version 90 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program