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P03015

- GIN_BPMU

UniProt

P03015 - GIN_BPMU

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Protein

Serine recombinase gin

Gene

gin

Organism
Enterobacteria phage Mu (Bacteriophage Mu)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Performs inversion of a viral 3 kp segment (G-segment) that encodes two alternate pairs of tail fiber proteins thereby modifying the host specificity of the virus. Binds as a dimer to the viral gix sites which are 34-bp palindromic sequences that flank the invertible G-segment. Catalyzes site-specific recombination in the presence of the host factor Fis. Gin dimers bound to each of the gix sites and host factor Fis bound to the enhancer come together to form the synaptic complex. Each Gin monomer introduces a nick and becomes covalently attached to the 5'-phosphate of the DNA, resulting in double-stranded staggered breaks at both recombination sites. A 180 degrees rotation of one of the two Gin dimers followed by religation of the DNA leads to the inversion of the G-segment (G+ or G- orientation).4 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei9 – 91O-(5'-phospho-DNA)-serine intermediate1 PublicationPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi138 – 18346H-T-H motifAdd
BLAST

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. hydrolase activity Source: UniProtKB-KW
  3. ligase activity Source: UniProtKB-KW
  4. recombinase activity Source: UniProtKB-KW

GO - Biological processi

  1. DNA integration Source: UniProtKB-KW
  2. viral entry into host cell Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

DNA invertase, Hydrolase, Ligase

Keywords - Biological processi

DNA integration, DNA recombination, Viral host range expansion by genetic inversion, Virus entry into host cell

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine recombinase gin (EC:3.1.22.-, EC:6.5.1.-)
Alternative name(s):
G-segment invertase
Short name:
Gin
Gene product 53
Short name:
gp53
Gene namesi
Name:gin
Ordered Locus Names:Mup53
OrganismiEnterobacteria phage Mu (Bacteriophage Mu)
Taxonomic identifieri10677 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesMyoviridaeMulikevirus
Virus hostiEnterobacteriaceae [TaxID: 543]
ProteomesiUP000002611: Genome

Subcellular locationi

GO - Cellular componenti

  1. host cell cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi9 – 91S → A, L or T: Recombination deficient. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 193193Serine recombinase ginPRO_0000196354Add
BLAST

Expressioni

Inductioni

Expressed in the late phase of the viral replicative cycle. Expression of late genes is activated by the viral late transcription activator C.1 Publication

Keywords - Developmental stagei

Late protein

Interactioni

Subunit structurei

Homodimer. During inversion, two dimers associate to form a homotetramer.2 Publications

Structurei

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3UJ3X-ray3.80X1-193[»]
4M6FX-ray4.99A1-193[»]
ProteinModelPortaliP03015.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The dimerization region is in the N-terminus.

Sequence similaritiesi

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
3.40.50.1390. 1 hit.
InterProiIPR009057. Homeodomain-like.
IPR006118. Recombinase_CS.
IPR006119. Resolv_N.
IPR006120. Resolvase_HTH_dom.
[Graphical view]
PfamiPF02796. HTH_7. 1 hit.
PF00239. Resolvase. 1 hit.
[Graphical view]
SMARTiSM00857. Resolvase. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF53041. SSF53041. 1 hit.
PROSITEiPS00397. RECOMBINASES_1. 1 hit.
PS00398. RECOMBINASES_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P03015-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLIGYVRVST NDQNTDLQRN ALVCAGCEQI FEDKLSGTRT DRPGLKRALK
60 70 80 90 100
RLQKGDTLVV WKLDRLGRSM KHLISLVGEL RERGINFRSL TDSIDTSSPM
110 120 130 140 150
GRFFFHVMGA LAEMERELII ERTMAGLAAA RNKGRIGGRP PKLTKAEWEQ
160 170 180 190
AGRLLAQGIP RKQVALIYDV ALSTLYKKHP AKRAHIENDD RIN
Length:193
Mass (Da):21,766
Last modified:December 1, 2000 - v2
Checksum:i86ED5D1C6ADD17DB
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti48 – 481A → D no nucleotide entry (PubMed:6310572)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF083977 Genomic DNA. Translation: AAF01129.1.
V01463 Genomic DNA. Translation: CAA24708.1.
PIRiS02705. JWBPU.
RefSeqiNP_050655.1. NC_000929.1.

Genome annotation databases

GeneIDi2636258.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF083977 Genomic DNA. Translation: AAF01129.1 .
V01463 Genomic DNA. Translation: CAA24708.1 .
PIRi S02705. JWBPU.
RefSeqi NP_050655.1. NC_000929.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3UJ3 X-ray 3.80 X 1-193 [» ]
4M6F X-ray 4.99 A 1-193 [» ]
ProteinModelPortali P03015.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 2636258.

Family and domain databases

Gene3Di 1.10.10.60. 1 hit.
3.40.50.1390. 1 hit.
InterProi IPR009057. Homeodomain-like.
IPR006118. Recombinase_CS.
IPR006119. Resolv_N.
IPR006120. Resolvase_HTH_dom.
[Graphical view ]
Pfami PF02796. HTH_7. 1 hit.
PF00239. Resolvase. 1 hit.
[Graphical view ]
SMARTi SM00857. Resolvase. 1 hit.
[Graphical view ]
SUPFAMi SSF46689. SSF46689. 1 hit.
SSF53041. SSF53041. 1 hit.
PROSITEi PS00397. RECOMBINASES_1. 1 hit.
PS00398. RECOMBINASES_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "DNA inversions in the chromosome of Escherichia coli and in bacteriophage Mu: relationship to other site-specific recombination systems."
    Plasterk R.H.A., Brinkman A., van de Putte P.
    Proc. Natl. Acad. Sci. U.S.A. 80:5355-5358(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Bacteriophage Mu genome sequence: analysis and comparison with Mu-like prophages in Haemophilus, Neisseria and Deinococcus."
    Morgan G.J., Hatfull G.F., Casjens S., Hendrix R.W.
    J. Mol. Biol. 317:337-359(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "Methylation regulates the expression of a DNA-modification function encoded by bacteriophage Mu."
    Kahmann R.
    Cold Spring Harb. Symp. Quant. Biol. 47:639-646(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 173-193.
  4. "The DNA invertase Gin of phage Mu: formation of a covalent complex with DNA via a phosphoserine at amino acid position 9."
    Klippel A., Mertens G., Patschinsky T., Kahmann R.
    EMBO J. 7:1229-1237(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-16, COVALENT COMPLEX WITH DNA, DNA-BINDING, ACTIVE SITE, MUTAGENESIS OF SER-9.
  5. "A genetic switch in vitro: DNA inversion by Gin protein of phage Mu."
    Plasterk R.H., Kanaar R., van de Putte P.
    Proc. Natl. Acad. Sci. U.S.A. 81:2689-2692(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "G inversion in bacteriophage Mu DNA is stimulated by a site within the invertase gene and a host factor."
    Kahmann R., Rudt F., Koch C., Mertens G.
    Cell 41:771-780(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Isolation and characterization of unusual gin mutants."
    Klippel A., Cloppenborg K., Kahmann R.
    EMBO J. 7:3983-3989(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF MUTANTS.
  8. "Site-specific recombination in bacteriophage Mu: characterization of binding sites for the DNA invertase Gin."
    Mertens G., Klippel A., Fuss H., Blocker H., Frank R., Kahmann R.
    EMBO J. 7:1219-1227(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: DNA-BINDING.
  9. "Mutational analysis of a C-dependent late promoter of bacteriophage Mu."
    Chiang L.W., Howe M.M.
    Genetics 135:619-629(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  10. "Gin invertase of bacteriophage Mu is a dimer in solution, with the domain for dimerization in the N-terminal part of the protein."
    Spaeny-Dekking L., van Hemert M., van de Putte P., Goosen N.
    Biochemistry 34:1779-1786(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT, REGION OF DIMERIZATION.
  11. "Crystal structure of an intermediate of rotating dimers within the synaptic tetramer of the G-segment invertase."
    Ritacco C.J., Kamtekar S., Wang J., Steitz T.A.
    Nucleic Acids Res. 41:2673-2682(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.8 ANGSTROMS) OF VAL-114 MUTANT, DNA-BINDING, SUBUNIT, FUNCTION.

Entry informationi

Entry nameiGIN_BPMU
AccessioniPrimary (citable) accession number: P03015
Secondary accession number(s): Q9T1U8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: December 1, 2000
Last modified: October 29, 2014
This is version 95 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

The orientation of the G segment is defined as G+ and G-. G+ orientation provides S-U fibers whereas G- provides S'-U' fibers. S-U and S'-U' dont have the same host range (e.g. respectively E.coli and C.freundii).

Caution

Translation initiates from a non-canonical start codon (GUG).Curated

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3