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P03015 (DNIV_BPMU) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA-invertase
Gene names
Name:gin
Synonyms:51
OrganismEnterobacteria phage Mu (Bacteriophage Mu) [Reference proteome]
Taxonomic identifier10677 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stageCaudoviralesMyoviridaeMulikevirus
Virus hostEnterobacteriaceae [TaxID: 543]

Protein attributes

Sequence length193 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This protein catalyzes the inversion of a 3000-bp segment of phage DNA. The inversion results in a modification of the 3'-end of the tail fiber gene and alters the host specificity. Ref.5

Sequence similarities

Belongs to the site-specific recombinase resolvase family.

Ontologies

Keywords
   Biological processDNA integration
DNA recombination
   LigandDNA-binding
   Molecular functionDNA invertase
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processDNA integration

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

recombinase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 193193DNA-invertase
PRO_0000196354

Regions

DNA binding161 – 18020H-T-H motif Potential

Sites

Active site91O-(5'-phospho-DNA)-serine intermediate

Experimental info

Sequence conflict481A → D Ref.1

Sequences

Sequence LengthMass (Da)Tools
P03015 [UniParc].

Last modified December 1, 2000. Version 2.
Checksum: 86ED5D1C6ADD17DB

FASTA19321,766
        10         20         30         40         50         60 
MLIGYVRVST NDQNTDLQRN ALVCAGCEQI FEDKLSGTRT DRPGLKRALK RLQKGDTLVV 

        70         80         90        100        110        120 
WKLDRLGRSM KHLISLVGEL RERGINFRSL TDSIDTSSPM GRFFFHVMGA LAEMERELII 

       130        140        150        160        170        180 
ERTMAGLAAA RNKGRIGGRP PKLTKAEWEQ AGRLLAQGIP RKQVALIYDV ALSTLYKKHP 

       190 
AKRAHIENDD RIN 

« Hide

References

« Hide 'large scale' references
[1]"DNA inversions in the chromosome of Escherichia coli and in bacteriophage Mu: relationship to other site-specific recombination systems."
Plasterk R.H.A., Brinkman A., van de Putte P.
Proc. Natl. Acad. Sci. U.S.A. 80:5355-5358(1983) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Bacteriophage Mu genome sequence: analysis and comparison with Mu-like prophages in Haemophilus, Neisseria and Deinococcus."
Morgan G.J., Hatfull G.F., Casjens S., Hendrix R.W.
J. Mol. Biol. 317:337-359(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"Methylation regulates the expression of a DNA-modification function encoded by bacteriophage Mu."
Kahmann R.
Cold Spring Harb. Symp. Quant. Biol. 47:639-646(1983) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 173-193.
[4]"The DNA invertase Gin of phage Mu: formation of a covalent complex with DNA via a phosphoserine at amino acid position 9."
Klippel A., Mertens G., Patschinsky T., Kahmann R.
EMBO J. 7:1229-1237(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-16, COVALENT COMPLEX WITH DNA.
[5]"Isolation and characterization of unusual gin mutants."
Klippel A., Cloppenborg K., Kahmann R.
EMBO J. 7:3983-3989(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION OF MUTANTS.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF083977 Genomic DNA. Translation: AAF01129.1.
V01463 Genomic DNA. Translation: CAA24708.1.
PIRJWBPU. S02705.
RefSeqNP_050655.1. NC_000929.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3UJ3X-ray3.80X1-193[»]
ProteinModelPortalP03015.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID2636258.

Phylogenomic databases

ProtClustDBCLSP2343583.

Family and domain databases

Gene3D1.10.10.60. 1 hit.
3.40.50.1390. 1 hit.
InterProIPR009057. Homeodomain-like.
IPR006118. Recombinase_CS.
IPR006119. Resolv_N.
IPR006120. Resolvase_HTH_dom.
[Graphical view]
PfamPF02796. HTH_7. 1 hit.
PF00239. Resolvase. 1 hit.
[Graphical view]
SMARTSM00857. Resolvase. 1 hit.
[Graphical view]
SUPFAMSSF46689. SSF46689. 1 hit.
SSF53041. SSF53041. 1 hit.
PROSITEPS00397. RECOMBINASES_1. 1 hit.
PS00398. RECOMBINASES_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDNIV_BPMU
AccessionPrimary (citable) accession number: P03015
Secondary accession number(s): Q9T1U8
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: December 1, 2000
Last modified: October 16, 2013
This is version 91 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references