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P03015

- GIN_BPMU

UniProt

P03015 - GIN_BPMU

Protein

Serine recombinase gin

Gene

gin

Organism
Enterobacteria phage Mu (Bacteriophage Mu)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 94 (01 Oct 2014)
      Sequence version 2 (01 Dec 2000)
      Previous versions | rss
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    Functioni

    Performs inversion of a viral 3 kp segment (G-segment) that encodes two alternate pairs of tail fiber proteins thereby modifying the host specificity of the virus. Binds as a dimer to the viral gix sites which are 34-bp palindromic sequences that flank the invertible G-segment. Catalyzes site-specific recombination in the presence of the host factor Fis. Gin dimers bound to each of the gix sites and host factor Fis bound to the enhancer come together to form the synaptic complex. Each Gin monomer introduces a nick and becomes covalently attached to the 5'-phosphate of the DNA, resulting in double-stranded staggered breaks at both recombination sites. A 180 degrees rotation of one of the two Gin dimers followed by religation of the DNA leads to the inversion of the G-segment (G+ or G- orientation).4 Publications

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei9 – 91O-(5'-phospho-DNA)-serine intermediate1 PublicationPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi138 – 18346H-T-H motifAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. hydrolase activity Source: UniProtKB-KW
    3. ligase activity Source: UniProtKB-KW
    4. recombinase activity Source: UniProtKB-KW

    GO - Biological processi

    1. DNA integration Source: UniProtKB-KW
    2. viral entry into host cell Source: UniProtKB-KW

    Keywords - Molecular functioni

    DNA invertase, Hydrolase, Ligase

    Keywords - Biological processi

    DNA integration, DNA recombination, Viral host range expansion by genetic inversion, Virus entry into host cell

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine recombinase gin (EC:3.1.22.-, EC:6.5.1.-)
    Alternative name(s):
    G-segment invertase
    Short name:
    Gin
    Gene product 53
    Short name:
    gp53
    Gene namesi
    Name:gin
    Ordered Locus Names:Mup53
    OrganismiEnterobacteria phage Mu (Bacteriophage Mu)
    Taxonomic identifieri10677 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stageCaudoviralesMyoviridaeMulikevirus
    Virus hostiEnterobacteriaceae [TaxID: 543]
    ProteomesiUP000002611: Genome

    Subcellular locationi

    GO - Cellular componenti

    1. host cell cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host cytoplasm

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi9 – 91S → A, L or T: Recombination deficient. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 193193Serine recombinase ginPRO_0000196354Add
    BLAST

    Expressioni

    Inductioni

    Expressed in the late phase of the viral replicative cycle. Expression of late genes is activated by the viral late transcription activator C.1 Publication

    Keywords - Developmental stagei

    Late protein

    Interactioni

    Subunit structurei

    Homodimer. During inversion, two dimers associate to form a homotetramer.2 Publications

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3UJ3X-ray3.80X1-193[»]
    4M6FX-ray4.99A1-193[»]
    ProteinModelPortaliP03015.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domaini

    The dimerization region is in the N-terminus.

    Sequence similaritiesi

    Family and domain databases

    Gene3Di1.10.10.60. 1 hit.
    3.40.50.1390. 1 hit.
    InterProiIPR009057. Homeodomain-like.
    IPR006118. Recombinase_CS.
    IPR006119. Resolv_N.
    IPR006120. Resolvase_HTH_dom.
    [Graphical view]
    PfamiPF02796. HTH_7. 1 hit.
    PF00239. Resolvase. 1 hit.
    [Graphical view]
    SMARTiSM00857. Resolvase. 1 hit.
    [Graphical view]
    SUPFAMiSSF46689. SSF46689. 1 hit.
    SSF53041. SSF53041. 1 hit.
    PROSITEiPS00397. RECOMBINASES_1. 1 hit.
    PS00398. RECOMBINASES_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P03015-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLIGYVRVST NDQNTDLQRN ALVCAGCEQI FEDKLSGTRT DRPGLKRALK    50
    RLQKGDTLVV WKLDRLGRSM KHLISLVGEL RERGINFRSL TDSIDTSSPM 100
    GRFFFHVMGA LAEMERELII ERTMAGLAAA RNKGRIGGRP PKLTKAEWEQ 150
    AGRLLAQGIP RKQVALIYDV ALSTLYKKHP AKRAHIENDD RIN 193
    Length:193
    Mass (Da):21,766
    Last modified:December 1, 2000 - v2
    Checksum:i86ED5D1C6ADD17DB
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti48 – 481A → D no nucleotide entry (PubMed:6310572)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF083977 Genomic DNA. Translation: AAF01129.1.
    V01463 Genomic DNA. Translation: CAA24708.1.
    PIRiS02705. JWBPU.
    RefSeqiNP_050655.1. NC_000929.1.

    Genome annotation databases

    GeneIDi2636258.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF083977 Genomic DNA. Translation: AAF01129.1 .
    V01463 Genomic DNA. Translation: CAA24708.1 .
    PIRi S02705. JWBPU.
    RefSeqi NP_050655.1. NC_000929.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3UJ3 X-ray 3.80 X 1-193 [» ]
    4M6F X-ray 4.99 A 1-193 [» ]
    ProteinModelPortali P03015.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 2636258.

    Family and domain databases

    Gene3Di 1.10.10.60. 1 hit.
    3.40.50.1390. 1 hit.
    InterProi IPR009057. Homeodomain-like.
    IPR006118. Recombinase_CS.
    IPR006119. Resolv_N.
    IPR006120. Resolvase_HTH_dom.
    [Graphical view ]
    Pfami PF02796. HTH_7. 1 hit.
    PF00239. Resolvase. 1 hit.
    [Graphical view ]
    SMARTi SM00857. Resolvase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46689. SSF46689. 1 hit.
    SSF53041. SSF53041. 1 hit.
    PROSITEi PS00397. RECOMBINASES_1. 1 hit.
    PS00398. RECOMBINASES_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "DNA inversions in the chromosome of Escherichia coli and in bacteriophage Mu: relationship to other site-specific recombination systems."
      Plasterk R.H.A., Brinkman A., van de Putte P.
      Proc. Natl. Acad. Sci. U.S.A. 80:5355-5358(1983) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Bacteriophage Mu genome sequence: analysis and comparison with Mu-like prophages in Haemophilus, Neisseria and Deinococcus."
      Morgan G.J., Hatfull G.F., Casjens S., Hendrix R.W.
      J. Mol. Biol. 317:337-359(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "Methylation regulates the expression of a DNA-modification function encoded by bacteriophage Mu."
      Kahmann R.
      Cold Spring Harb. Symp. Quant. Biol. 47:639-646(1983) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 173-193.
    4. "The DNA invertase Gin of phage Mu: formation of a covalent complex with DNA via a phosphoserine at amino acid position 9."
      Klippel A., Mertens G., Patschinsky T., Kahmann R.
      EMBO J. 7:1229-1237(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-16, COVALENT COMPLEX WITH DNA, DNA-BINDING, ACTIVE SITE, MUTAGENESIS OF SER-9.
    5. "A genetic switch in vitro: DNA inversion by Gin protein of phage Mu."
      Plasterk R.H., Kanaar R., van de Putte P.
      Proc. Natl. Acad. Sci. U.S.A. 81:2689-2692(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. "G inversion in bacteriophage Mu DNA is stimulated by a site within the invertase gene and a host factor."
      Kahmann R., Rudt F., Koch C., Mertens G.
      Cell 41:771-780(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "Isolation and characterization of unusual gin mutants."
      Klippel A., Cloppenborg K., Kahmann R.
      EMBO J. 7:3983-3989(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION OF MUTANTS.
    8. "Site-specific recombination in bacteriophage Mu: characterization of binding sites for the DNA invertase Gin."
      Mertens G., Klippel A., Fuss H., Blocker H., Frank R., Kahmann R.
      EMBO J. 7:1219-1227(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: DNA-BINDING.
    9. "Mutational analysis of a C-dependent late promoter of bacteriophage Mu."
      Chiang L.W., Howe M.M.
      Genetics 135:619-629(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    10. "Gin invertase of bacteriophage Mu is a dimer in solution, with the domain for dimerization in the N-terminal part of the protein."
      Spaeny-Dekking L., van Hemert M., van de Putte P., Goosen N.
      Biochemistry 34:1779-1786(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBUNIT, REGION OF DIMERIZATION.
    11. "Crystal structure of an intermediate of rotating dimers within the synaptic tetramer of the G-segment invertase."
      Ritacco C.J., Kamtekar S., Wang J., Steitz T.A.
      Nucleic Acids Res. 41:2673-2682(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.8 ANGSTROMS) OF VAL-114 MUTANT, DNA-BINDING, SUBUNIT, FUNCTION.

    Entry informationi

    Entry nameiGIN_BPMU
    AccessioniPrimary (citable) accession number: P03015
    Secondary accession number(s): Q9T1U8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: December 1, 2000
    Last modified: October 1, 2014
    This is version 94 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The orientation of the G segment is defined as G+ and G-. G+ orientation provides S-U fibers whereas G- provides S'-U' fibers. S-U and S'-U' dont have the same host range (e.g. respectively E.coli and C.freundii).

    Caution

    Translation initiates from a non-canonical start codon (GUG).Curated

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3