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Protein

Serine recombinase PinE

Gene

pinE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

This protein catalyzes the inversion of an 1800-bp E.coli DNA fragment, the P region, which can exist in either orientation. The function of the inversion is not yet clear.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei9O-(5'-phospho-DNA)-serine intermediatePROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi161 – 180H-T-H motifSequence analysisAdd BLAST20

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • hydrolase activity Source: UniProtKB-KW
  • ligase activity Source: UniProtKB-KW
  • recombinase activity Source: EcoCyc

GO - Biological processi

  • DNA integration Source: UniProtKB-KW
  • DNA recombination Source: EcoCyc

Keywordsi

Molecular functionDNA invertase, DNA-binding, Hydrolase, Ligase
Biological processDNA integration, DNA recombination

Enzyme and pathway databases

BioCyciEcoCyc:EG10737-MONOMER
MetaCyc:EG10737-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Serine recombinase PinEBy similarity (EC:3.1.22.-, EC:6.5.1.-)
Alternative name(s):
DNA-invertase PinECurated
Short name:
DNA-invertase from lambdoid prophage e14
Site-specific recombinase PinECurated
Gene namesi
Name:pinE
Synonyms:pin
Ordered Locus Names:b1158, JW1144
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10737 pinE

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001963601 – 184Serine recombinase PinEAdd BLAST184

Proteomic databases

PaxDbiP03014
PRIDEiP03014

Interactioni

Protein-protein interaction databases

BioGridi4262859, 144 interactors
IntActiP03014, 2 interactors
STRINGi316407.1651570

Structurei

3D structure databases

ProteinModelPortaliP03014
SMRiP03014
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 134Resolvase/invertase-type recombinase catalyticPROSITE-ProRule annotationAdd BLAST134

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4108C33 Bacteria
COG1961 LUCA
HOGENOMiHOG000275578
InParanoidiP03014
OMAiMALKMYD
PhylomeDBiP03014

Family and domain databases

InterProiView protein in InterPro
IPR009057 Homeobox-like_sf
IPR006118 Recombinase_CS
IPR006119 Resolv_N
IPR036162 Resolvase-like_N_sf
IPR006120 Resolvase_HTH_dom
PfamiView protein in Pfam
PF02796 HTH_7, 1 hit
PF00239 Resolvase, 1 hit
SMARTiView protein in SMART
SM00857 Resolvase, 1 hit
SUPFAMiSSF46689 SSF46689, 1 hit
SSF53041 SSF53041, 1 hit
PROSITEiView protein in PROSITE
PS00397 RECOMBINASES_1, 1 hit
PS00398 RECOMBINASES_2, 1 hit
PS51736 RECOMBINASES_3, 1 hit

Sequencei

Sequence statusi: Complete.

P03014-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLIGYVRVST NDQNTDLQRN ALNCAGCELI FEDKISGTKS ERPGLKKLLR
60 70 80 90 100
TLSAGDTLVV WKLDRLGRSM RHLVVLVEEL RERGINFRSL TDSIDTSTPM
110 120 130 140 150
GRFFFHVMGA LAEMERELIV ERTKAGLETA RAQGRIGGRR PKLTPEQWAQ
160 170 180
AGRLIAAGTP RQKVAIIYDV GVSTLYKRFP AGDK
Length:184
Mass (Da):20,573
Last modified:November 1, 1997 - v2
Checksum:i5CD5F23F1E21A1DE
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti178R → T in CAA25948 (PubMed:3894006).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K00676 Genomic DNA Translation: AAA24391.1
X01805 Genomic DNA Translation: CAA25948.1
U00096 Genomic DNA Translation: AAC74242.1
AP009048 Genomic DNA Translation: BAA35994.1
PIRiA03545 JWEC
RefSeqiNP_415676.1, NC_000913.3
WP_000905001.1, NZ_CP010440.1

Genome annotation databases

EnsemblBacteriaiAAC74242; AAC74242; b1158
BAA35994; BAA35994; BAA35994
GeneIDi945721
KEGGiecj:JW1144
eco:b1158
PATRICifig|511145.12.peg.1199

Similar proteinsi

Entry informationi

Entry nameiPINE_ECOLI
AccessioniPrimary (citable) accession number: P03014
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: November 1, 1997
Last modified: March 28, 2018
This is version 140 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health