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Protein

DNA-invertase from lambdoid prophage e14

Gene

pinE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

This protein catalyzes the inversion of an 1800-bp E.coli DNA fragment, the P region, which can exist in either orientation. The function of the inversion is not yet clear.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei9 – 91O-(5'-phospho-DNA)-serine intermediatePROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi161 – 18020H-T-H motifSequence analysisAdd
BLAST

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • recombinase activity Source: EcoCyc

GO - Biological processi

  • DNA integration Source: UniProtKB-KW
  • DNA recombination Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

DNA invertase

Keywords - Biological processi

DNA integration, DNA recombination

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG10737-MONOMER.
ECOL316407:JW1144-MONOMER.
MetaCyc:EG10737-MONOMER.
RETL1328306-WGS:GSTH-4163-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-invertase from lambdoid prophage e14
Gene namesi
Name:pinE
Synonyms:pin
Ordered Locus Names:b1158, JW1144
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10737. pinE.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 184184DNA-invertase from lambdoid prophage e14PRO_0000196360Add
BLAST

Proteomic databases

PaxDbiP03014.
PRIDEiP03014.

Interactioni

Protein-protein interaction databases

BioGridi4262859. 142 interactions.
IntActiP03014. 2 interactions.
STRINGi511145.b1158.

Structurei

3D structure databases

ProteinModelPortaliP03014.
SMRiP03014. Positions 2-181.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 134134Resolvase/invertase-type recombinase catalyticPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 resolvase/invertase-type recombinase catalytic domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4108C33. Bacteria.
COG1961. LUCA.
HOGENOMiHOG000275578.
InParanoidiP03014.
OMAiRWQIANI.
PhylomeDBiP03014.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
3.40.50.1390. 1 hit.
InterProiIPR009057. Homeodomain-like.
IPR006118. Recombinase_CS.
IPR006119. Resolv_N.
IPR006120. Resolvase_HTH_dom.
[Graphical view]
PfamiPF02796. HTH_7. 1 hit.
PF00239. Resolvase. 1 hit.
[Graphical view]
SMARTiSM00857. Resolvase. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF53041. SSF53041. 1 hit.
PROSITEiPS00397. RECOMBINASES_1. 1 hit.
PS00398. RECOMBINASES_2. 1 hit.
PS51736. RECOMBINASES_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P03014-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLIGYVRVST NDQNTDLQRN ALNCAGCELI FEDKISGTKS ERPGLKKLLR
60 70 80 90 100
TLSAGDTLVV WKLDRLGRSM RHLVVLVEEL RERGINFRSL TDSIDTSTPM
110 120 130 140 150
GRFFFHVMGA LAEMERELIV ERTKAGLETA RAQGRIGGRR PKLTPEQWAQ
160 170 180
AGRLIAAGTP RQKVAIIYDV GVSTLYKRFP AGDK
Length:184
Mass (Da):20,573
Last modified:November 1, 1997 - v2
Checksum:i5CD5F23F1E21A1DE
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti178 – 1781R → T in CAA25948 (PubMed:3894006).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K00676 Genomic DNA. Translation: AAA24391.1.
X01805 Genomic DNA. Translation: CAA25948.1.
U00096 Genomic DNA. Translation: AAC74242.1.
AP009048 Genomic DNA. Translation: BAA35994.1.
PIRiA03545. JWEC.
RefSeqiNP_415676.1. NC_000913.3.
WP_000905001.1. NZ_CP010440.1.

Genome annotation databases

EnsemblBacteriaiAAC74242; AAC74242; b1158.
BAA35994; BAA35994; BAA35994.
GeneIDi945721.
KEGGiecj:JW1144.
eco:b1158.
PATRICi32117553. VBIEscCol129921_1199.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K00676 Genomic DNA. Translation: AAA24391.1.
X01805 Genomic DNA. Translation: CAA25948.1.
U00096 Genomic DNA. Translation: AAC74242.1.
AP009048 Genomic DNA. Translation: BAA35994.1.
PIRiA03545. JWEC.
RefSeqiNP_415676.1. NC_000913.3.
WP_000905001.1. NZ_CP010440.1.

3D structure databases

ProteinModelPortaliP03014.
SMRiP03014. Positions 2-181.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262859. 142 interactions.
IntActiP03014. 2 interactions.
STRINGi511145.b1158.

Proteomic databases

PaxDbiP03014.
PRIDEiP03014.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74242; AAC74242; b1158.
BAA35994; BAA35994; BAA35994.
GeneIDi945721.
KEGGiecj:JW1144.
eco:b1158.
PATRICi32117553. VBIEscCol129921_1199.

Organism-specific databases

EchoBASEiEB0730.
EcoGeneiEG10737. pinE.

Phylogenomic databases

eggNOGiENOG4108C33. Bacteria.
COG1961. LUCA.
HOGENOMiHOG000275578.
InParanoidiP03014.
OMAiRWQIANI.
PhylomeDBiP03014.

Enzyme and pathway databases

BioCyciEcoCyc:EG10737-MONOMER.
ECOL316407:JW1144-MONOMER.
MetaCyc:EG10737-MONOMER.
RETL1328306-WGS:GSTH-4163-MONOMER.

Miscellaneous databases

PROiP03014.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
3.40.50.1390. 1 hit.
InterProiIPR009057. Homeodomain-like.
IPR006118. Recombinase_CS.
IPR006119. Resolv_N.
IPR006120. Resolvase_HTH_dom.
[Graphical view]
PfamiPF02796. HTH_7. 1 hit.
PF00239. Resolvase. 1 hit.
[Graphical view]
SMARTiSM00857. Resolvase. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF53041. SSF53041. 1 hit.
PROSITEiPS00397. RECOMBINASES_1. 1 hit.
PS00398. RECOMBINASES_2. 1 hit.
PS51736. RECOMBINASES_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPINE_ECOLI
AccessioniPrimary (citable) accession number: P03014
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: November 1, 1997
Last modified: September 7, 2016
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.