Transposon gamma-delta resolvase - Escherichia coli (strain K12)

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P03012 (TNR1_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified August 10, 2010. Version 90. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Customize display Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Names and origin

Protein names

Recommended name:
Transposon gamma-delta resolvase

Alternative name(s):
Transposon Tn1000 resolvase

Gene names

Name:	tnpR
Ordered Locus Names:	ECOK12F009

Encoded on

Plasmid F Ref.4

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	183 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	This protein catalyzes the site-specific recombination of the transposon and also regulates its frequency of transposition.
Sequence similarities	Belongs to the site-specific recombinase resolvase family.

Ontologies

Keywords
Biological process	DNA integration DNA recombination
Ligand	DNA-binding
Technical term	3D-structure Complete proteome Plasmid Transposable element
Gene Ontology (GO)
Biological process	DNA integration Inferred from electronic annotation. Source: UniProtKB-KW DNA recombination Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription Inferred from electronic annotation. Source: InterPro
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW recombinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 183

183

Transposon gamma-delta resolvase

PRO_0000196367

Regions

DNA binding

161 – 180

H-T-H motif Potential

Sites

Active site

O-(5'-phospho-DNA)-serine intermediate Ref.5

Secondary structure

183

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P03012-1 [UniParc].

Last modified July 21, 1986. Version 1.
Checksum: B2EBA6037F926FAB
Show »

FASTA

183

20,362

        10         20         30         40         50         60 
MRLFGYARVS TSQQSLDIQV RALKDAGVKA NRIFTDKASG SSSDRKGLDL LRMKVEEGDV 

        70         80         90        100        110        120 
ILVKKLDRLG RDTADMIQLI KEFDAQGVSI RFIDDGISTD GEMGKMVVTI LSAVAQAERQ 

       130        140        150        160        170        180 
RILERTNEGR QEAMAKGVVF GRKRKIDRDA VLNMWQQGLG ASHISKTMNI ARSTVYKVIN 


ESN

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of gamma delta resolvase gene and demonstration that its gene product acts as a repressor of transcription." Reed R.R., Shibuya G.I., Steitz J.A. Nature 300:381-383(1982) [PubMed: 6292730] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Identification of the region that determines the specificity of binding of the transposases encoded by Tn3 and gamma delta to the terminal inverted repeat sequences." Maekawa T., Ohtsubo E. Jpn. J. Genet. 69:269-285(1994) [PubMed: 8080658] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Sequence of a transposon identified as Tn1000 (gamma delta)." Broom J.E., Hill D.F., Hughes G., Jones W.A., McNaughton J.C., Stockwell P.A., Petersen G.B. DNA Seq. 5:185-189(1995) [PubMed: 7612932] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Complete nucleotide sequence of the F plasmid: its implications for organization and diversification of plasmid genomes." Shimizu H., Saitoh Y., Suda Y., Uehara K., Sampei G., Mizobuchi K. Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / CR63.
[5]	"Resolvase-mediated recombination intermediates contain a serine residue covalently linked to DNA." Reed R.R., Moser C.D. Cold Spring Harb. Symp. Quant. Biol. 49:245-249(1984) [PubMed: 6099239] [Abstract] Cited for: ACTIVE SITE SER-10.
[6]	"Mutants of the gamma delta resolvase: a genetic analysis of the recombination function." Newman B.J., Grindley N.D.F. Cell 38:463-469(1984) [PubMed: 6088082] [Abstract] Cited for: MUTAGENESIS.
[7]	"The crystal structure of the catalytic domain of the site-specific recombination enzyme gamma delta resolvase at 2.7-A resolution." Sanderson M.R., Freemont P.S., Rice P.A., Goldman A., Hatfull G.F., Grindley N.D.F., Steitz T.A. Cell 63:1323-1329(1990) [PubMed: 2175678] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 1-140.
[8]	"Crystal structure of the site-specific recombinase gamma delta resolvase complexed with a 34 bp cleavage site." Yang W., Steitz T.A. Cell 82:193-207(1995) [PubMed: 7628011] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS).
[9]	"Determination of the structure of the DNA binding domain of gamma delta resolvase in solution." Liu T., Derose E.F., Mullen G.P. Protein Sci. 3:1286-1295(1994) [PubMed: 7987224] [Abstract] Cited for: STRUCTURE BY NMR OF 141-183.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

J01844 Genomic DNA. No translation available.
X60200 Genomic DNA. Translation: CAA42759.1.
D16449 Genomic DNA. Translation: BAA03915.1.
AP001918 Genomic DNA. Translation: BAA97879.1.

PIR

RPECTG. A03542.

RefSeq

NP_061388.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1GDR	X-ray	3.50	A	1-140	[»]
1GDT	X-ray	3.00	A/B	1-183	[»]
1GHT	NMR	-	A	1-105	[»]
1HX7	NMR	-	A	1-105	[»]
1RES	NMR	-	A	141-183	[»]
1RET	NMR	-	A	141-183	[»]
1ZR2	X-ray	3.90	A/B	3-183	[»]
1ZR4	X-ray	3.40	A/B/D/E	3-183	[»]
2GM4	X-ray	3.50	A/B	3-183	[»]
2GM5	X-ray	2.10	A/B/C/D	3-134	[»]
2RSL	X-ray	2.30	A/B/C	1-140	[»]

ProteinModelPortal

P03012.

ModBase

Search...

Genome annotation databases

EnsemblBacteria

EBESCT00000005433; EBESCP00000005120; EBESCG00000004497.

GeneID

1263577.

GenomeReviews

Gene locus ECOK12F009 in contig AP001918_GR.

Organism-specific databases

EcoGene

EG40024. tnpR.

CMR

Search...

Phylogenomic databases

eggNOG

COG1961.

HOGENOM

HBG739917.

OMA

AMGKMVV.

ProtClustDB

CLSK861849.

Gene expression databases

Genevestigator

P03012.

Family and domain databases

InterPro

IPR009057. Homeodomain-like.
IPR012287. Homeodomain-rel.
IPR006118. Recombinase_CS.
IPR006119. Resolv_N.
IPR006120. Resolvase_HTH_dom.
[Graphical view]

Gene3D

G3DSA:1.10.10.60. Homeodomain-rel. 1 hit.

Pfam

PF02796. HTH_7. 1 hit.
PF00239. Resolvase. 1 hit.
[Graphical view]

SMART

SM00857. Resolvase. 1 hit.
[Graphical view]

SUPFAM

SSF46689. Homeodomain_like. 1 hit.
SSF53041. Resolv_N. 1 hit.

PROSITE

PS00397. RECOMBINASES_1. 1 hit.
PS00398. RECOMBINASES_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

TNR1_ECOLI

Accession

Primary (citable) accession number: P03012

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	August 10, 2010
This is version 90 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Customize display

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents