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P03012

- TNR1_ECOLI

UniProt

P03012 - TNR1_ECOLI

Protein

Transposon gamma-delta resolvase

Gene

tnpR

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 114 (01 Oct 2014)
      Sequence version 1 (21 Jul 1986)
      Previous versions | rss
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    Functioni

    This protein catalyzes the site-specific recombination of the transposon and also regulates its frequency of transposition.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei10 – 101O-(5'-phospho-DNA)-serine intermediate1 PublicationPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi161 – 18020H-T-H motifSequence AnalysisAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. recombinase activity Source: InterPro

    GO - Biological processi

    1. DNA integration Source: UniProtKB-KW

    Keywords - Biological processi

    DNA integration, DNA recombination

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transposon gamma-delta resolvase
    Alternative name(s):
    Transposon Tn1000 resolvase
    Gene namesi
    Name:tnpR
    Ordered Locus Names:ECOK12F009
    Encoded oniPlasmid F1 Publication
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 183183Transposon gamma-delta resolvasePRO_0000196367Add
    BLAST

    Proteomic databases

    PRIDEiP03012.

    Expressioni

    Gene expression databases

    GenevestigatoriP03012.

    Structurei

    Secondary structure

    1
    183
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi3 – 119
    Helixi13 – 2513
    Helixi30 – 323
    Beta strandi33 – 375
    Beta strandi41 – 433
    Helixi46 – 549
    Beta strandi60 – 656
    Helixi66 – 683
    Beta strandi69 – 724
    Helixi73 – 8513
    Beta strandi89 – 924
    Turni93 – 964
    Helixi102 – 12726
    Turni135 – 1373
    Beta strandi143 – 1453
    Helixi148 – 1569
    Helixi161 – 1688
    Helixi172 – 1809

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1GDRX-ray3.50A1-140[»]
    1GDTX-ray3.00A/B1-183[»]
    1GHTNMR-A1-105[»]
    1HX7NMR-A1-105[»]
    1RESNMR-A141-183[»]
    1RETNMR-A141-183[»]
    1ZR2X-ray3.90A/B1-183[»]
    1ZR4X-ray3.40A/B/D/E1-183[»]
    2GM4X-ray3.50A/B1-183[»]
    2GM5X-ray2.10A/B/C/D2-134[»]
    2RSLX-ray2.30A/B/C1-140[»]
    ProteinModelPortaliP03012.
    SMRiP03012. Positions 1-183.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP03012.

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    HOGENOMiHOG000275578.
    OMAiYIFMRLF.
    PhylomeDBiP03012.

    Family and domain databases

    Gene3Di1.10.10.60. 1 hit.
    3.40.50.1390. 1 hit.
    InterProiIPR009057. Homeodomain-like.
    IPR006118. Recombinase_CS.
    IPR006119. Resolv_N.
    IPR006120. Resolvase_HTH_dom.
    [Graphical view]
    PfamiPF02796. HTH_7. 1 hit.
    PF00239. Resolvase. 1 hit.
    [Graphical view]
    SMARTiSM00857. Resolvase. 1 hit.
    [Graphical view]
    SUPFAMiSSF46689. SSF46689. 1 hit.
    SSF53041. SSF53041. 1 hit.
    PROSITEiPS00397. RECOMBINASES_1. 1 hit.
    PS00398. RECOMBINASES_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P03012-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRLFGYARVS TSQQSLDIQV RALKDAGVKA NRIFTDKASG SSSDRKGLDL    50
    LRMKVEEGDV ILVKKLDRLG RDTADMIQLI KEFDAQGVSI RFIDDGISTD 100
    GEMGKMVVTI LSAVAQAERQ RILERTNEGR QEAMAKGVVF GRKRKIDRDA 150
    VLNMWQQGLG ASHISKTMNI ARSTVYKVIN ESN 183
    Length:183
    Mass (Da):20,362
    Last modified:July 21, 1986 - v1
    Checksum:iB2EBA6037F926FAB
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J01844 Genomic DNA. No translation available.
    X60200 Genomic DNA. Translation: CAA42759.1.
    D16449 Genomic DNA. Translation: BAA03915.1.
    AP001918 Genomic DNA. Translation: BAA97879.1.
    PIRiA03542. RPECTG.
    RefSeqiNP_061388.1. NC_002483.1.
    WP_001235704.1. NC_002483.1.

    Genome annotation databases

    GeneIDi1263577.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J01844 Genomic DNA. No translation available.
    X60200 Genomic DNA. Translation: CAA42759.1 .
    D16449 Genomic DNA. Translation: BAA03915.1 .
    AP001918 Genomic DNA. Translation: BAA97879.1 .
    PIRi A03542. RPECTG.
    RefSeqi NP_061388.1. NC_002483.1.
    WP_001235704.1. NC_002483.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1GDR X-ray 3.50 A 1-140 [» ]
    1GDT X-ray 3.00 A/B 1-183 [» ]
    1GHT NMR - A 1-105 [» ]
    1HX7 NMR - A 1-105 [» ]
    1RES NMR - A 141-183 [» ]
    1RET NMR - A 141-183 [» ]
    1ZR2 X-ray 3.90 A/B 1-183 [» ]
    1ZR4 X-ray 3.40 A/B/D/E 1-183 [» ]
    2GM4 X-ray 3.50 A/B 1-183 [» ]
    2GM5 X-ray 2.10 A/B/C/D 2-134 [» ]
    2RSL X-ray 2.30 A/B/C 1-140 [» ]
    ProteinModelPortali P03012.
    SMRi P03012. Positions 1-183.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi P03012.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 1263577.

    Phylogenomic databases

    HOGENOMi HOG000275578.
    OMAi YIFMRLF.
    PhylomeDBi P03012.

    Miscellaneous databases

    EvolutionaryTracei P03012.
    PROi P03012.

    Gene expression databases

    Genevestigatori P03012.

    Family and domain databases

    Gene3Di 1.10.10.60. 1 hit.
    3.40.50.1390. 1 hit.
    InterProi IPR009057. Homeodomain-like.
    IPR006118. Recombinase_CS.
    IPR006119. Resolv_N.
    IPR006120. Resolvase_HTH_dom.
    [Graphical view ]
    Pfami PF02796. HTH_7. 1 hit.
    PF00239. Resolvase. 1 hit.
    [Graphical view ]
    SMARTi SM00857. Resolvase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46689. SSF46689. 1 hit.
    SSF53041. SSF53041. 1 hit.
    PROSITEi PS00397. RECOMBINASES_1. 1 hit.
    PS00398. RECOMBINASES_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of gamma delta resolvase gene and demonstration that its gene product acts as a repressor of transcription."
      Reed R.R., Shibuya G.I., Steitz J.A.
      Nature 300:381-383(1982) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Identification of the region that determines the specificity of binding of the transposases encoded by Tn3 and gamma delta to the terminal inverted repeat sequences."
      Maekawa T., Ohtsubo E.
      Jpn. J. Genet. 69:269-285(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Sequence of a transposon identified as Tn1000 (gamma delta)."
      Broom J.E., Hill D.F., Hughes G., Jones W.A., McNaughton J.C., Stockwell P.A., Petersen G.B.
      DNA Seq. 5:185-189(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    4. "Complete nucleotide sequence of the F plasmid: its implications for organization and diversification of plasmid genomes."
      Shimizu H., Saitoh Y., Suda Y., Uehara K., Sampei G., Mizobuchi K.
      Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / CR63.
      Plasmid: F
    5. "Resolvase-mediated recombination intermediates contain a serine residue covalently linked to DNA."
      Reed R.R., Moser C.D.
      Cold Spring Harb. Symp. Quant. Biol. 49:245-249(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACTIVE SITE SER-10.
    6. "Mutants of the gamma delta resolvase: a genetic analysis of the recombination function."
      Newman B.J., Grindley N.D.F.
      Cell 38:463-469(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS.
    7. "The crystal structure of the catalytic domain of the site-specific recombination enzyme gamma delta resolvase at 2.7-A resolution."
      Sanderson M.R., Freemont P.S., Rice P.A., Goldman A., Hatfull G.F., Grindley N.D.F., Steitz T.A.
      Cell 63:1323-1329(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 1-140.
    8. "Crystal structure of the site-specific recombinase gamma delta resolvase complexed with a 34 bp cleavage site."
      Yang W., Steitz T.A.
      Cell 82:193-207(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS).
    9. "Determination of the structure of the DNA binding domain of gamma delta resolvase in solution."
      Liu T., Derose E.F., Mullen G.P.
      Protein Sci. 3:1286-1295(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF 141-183.

    Entry informationi

    Entry nameiTNR1_ECOLI
    AccessioniPrimary (citable) accession number: P03012
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: July 21, 1986
    Last modified: October 1, 2014
    This is version 114 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Plasmid, Transposable element

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3