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Protein

Transposon gamma-delta resolvase

Gene

tnpR

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

This protein catalyzes the site-specific recombination of the transposon and also regulates its frequency of transposition.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei10O-(5'-phospho-DNA)-serine intermediatePROSITE-ProRule annotation1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi161 – 180H-T-H motifSequence analysisAdd BLAST20

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

DNA integration, DNA recombination

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciECOL316385:GJ8B-1333-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Transposon gamma-delta resolvase
Alternative name(s):
Transposon Tn1000 resolvase
Gene namesi
Name:tnpR
Ordered Locus Names:ECOK12F009
Encoded oniPlasmid F1 Publication
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001963671 – 183Transposon gamma-delta resolvaseAdd BLAST183

Proteomic databases

PRIDEiP03012.

Interactioni

Protein-protein interaction databases

DIPiDIP-61217N.
STRINGi316385.ECDH10B_1363.

Structurei

Secondary structure

1183
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 11Combined sources9
Helixi13 – 25Combined sources13
Helixi30 – 32Combined sources3
Beta strandi33 – 37Combined sources5
Beta strandi41 – 43Combined sources3
Helixi46 – 54Combined sources9
Beta strandi60 – 65Combined sources6
Helixi66 – 68Combined sources3
Beta strandi69 – 72Combined sources4
Helixi73 – 85Combined sources13
Beta strandi89 – 92Combined sources4
Turni93 – 96Combined sources4
Helixi102 – 127Combined sources26
Turni135 – 137Combined sources3
Beta strandi143 – 145Combined sources3
Helixi148 – 156Combined sources9
Helixi161 – 168Combined sources8
Helixi172 – 180Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GDRX-ray3.50A1-140[»]
1GDTX-ray3.00A/B1-183[»]
1GHTNMR-A1-105[»]
1HX7NMR-A1-105[»]
1RESNMR-A141-183[»]
1RETNMR-A141-183[»]
1ZR2X-ray3.90A/B1-183[»]
1ZR4X-ray3.40A/B/D/E1-183[»]
2GM4X-ray3.50A/B1-183[»]
2GM5X-ray2.10A/B/C/D2-134[»]
2RSLX-ray2.30A/B/C1-140[»]
ProteinModelPortaliP03012.
SMRiP03012.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP03012.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 137Resolvase/invertase-type recombinase catalyticPROSITE-ProRule annotationAdd BLAST136

Sequence similaritiesi

Contains 1 resolvase/invertase-type recombinase catalytic domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4108C33. Bacteria.
COG1961. LUCA.
HOGENOMiHOG000275578.
OMAiRKQGMTY.
PhylomeDBiP03012.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
3.40.50.1390. 1 hit.
InterProiIPR009057. Homeodomain-like.
IPR006118. Recombinase_CS.
IPR006119. Resolv_N.
IPR006120. Resolvase_HTH_dom.
[Graphical view]
PfamiPF02796. HTH_7. 1 hit.
PF00239. Resolvase. 1 hit.
[Graphical view]
SMARTiSM00857. Resolvase. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF53041. SSF53041. 1 hit.
PROSITEiPS00397. RECOMBINASES_1. 1 hit.
PS00398. RECOMBINASES_2. 1 hit.
PS51736. RECOMBINASES_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P03012-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLFGYARVS TSQQSLDIQV RALKDAGVKA NRIFTDKASG SSSDRKGLDL
60 70 80 90 100
LRMKVEEGDV ILVKKLDRLG RDTADMIQLI KEFDAQGVSI RFIDDGISTD
110 120 130 140 150
GEMGKMVVTI LSAVAQAERQ RILERTNEGR QEAMAKGVVF GRKRKIDRDA
160 170 180
VLNMWQQGLG ASHISKTMNI ARSTVYKVIN ESN
Length:183
Mass (Da):20,362
Last modified:July 21, 1986 - v1
Checksum:iB2EBA6037F926FAB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01844 Genomic DNA. No translation available.
X60200 Genomic DNA. Translation: CAA42759.1.
D16449 Genomic DNA. Translation: BAA03915.1.
AP001918 Genomic DNA. Translation: BAA97879.1.
PIRiA03542. RPECTG.
RefSeqiNP_061388.1. NC_002483.1.
WP_001235704.1. NZ_CP014273.1.

Genome annotation databases

GeneIDi1263577.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01844 Genomic DNA. No translation available.
X60200 Genomic DNA. Translation: CAA42759.1.
D16449 Genomic DNA. Translation: BAA03915.1.
AP001918 Genomic DNA. Translation: BAA97879.1.
PIRiA03542. RPECTG.
RefSeqiNP_061388.1. NC_002483.1.
WP_001235704.1. NZ_CP014273.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GDRX-ray3.50A1-140[»]
1GDTX-ray3.00A/B1-183[»]
1GHTNMR-A1-105[»]
1HX7NMR-A1-105[»]
1RESNMR-A141-183[»]
1RETNMR-A141-183[»]
1ZR2X-ray3.90A/B1-183[»]
1ZR4X-ray3.40A/B/D/E1-183[»]
2GM4X-ray3.50A/B1-183[»]
2GM5X-ray2.10A/B/C/D2-134[»]
2RSLX-ray2.30A/B/C1-140[»]
ProteinModelPortaliP03012.
SMRiP03012.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-61217N.
STRINGi316385.ECDH10B_1363.

Proteomic databases

PRIDEiP03012.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1263577.

Phylogenomic databases

eggNOGiENOG4108C33. Bacteria.
COG1961. LUCA.
HOGENOMiHOG000275578.
OMAiRKQGMTY.
PhylomeDBiP03012.

Enzyme and pathway databases

BioCyciECOL316385:GJ8B-1333-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP03012.
PROiP03012.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
3.40.50.1390. 1 hit.
InterProiIPR009057. Homeodomain-like.
IPR006118. Recombinase_CS.
IPR006119. Resolv_N.
IPR006120. Resolvase_HTH_dom.
[Graphical view]
PfamiPF02796. HTH_7. 1 hit.
PF00239. Resolvase. 1 hit.
[Graphical view]
SMARTiSM00857. Resolvase. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF53041. SSF53041. 1 hit.
PROSITEiPS00397. RECOMBINASES_1. 1 hit.
PS00398. RECOMBINASES_2. 1 hit.
PS51736. RECOMBINASES_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTNR1_ECOLI
AccessioniPrimary (citable) accession number: P03012
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: November 30, 2016
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Plasmid, Transposable element

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.