Transposon gamma-delta resolvase - Escherichia coli (strain K12)

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P03012 (TNR1_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 108. History...

Clusters with 100%, 90%, 50% identity |

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Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Transposon gamma-delta resolvase

Alternative name(s):
Transposon Tn1000 resolvase

Gene names

Name:	tnpR
Ordered Locus Names:	ECOK12F009

Encoded on

Plasmid F Ref.4

Organism

Escherichia coli (strain K12)

Taxonomic identifier

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length	183 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This protein catalyzes the site-specific recombination of the transposon and also regulates its frequency of transposition.
Sequence similarities	Belongs to the site-specific recombinase resolvase family.

Ontologies

Keywords
Biological process	DNA integration DNA recombination
Ligand	DNA-binding
Technical term	3D-structure Plasmid Transposable element
Gene Ontology (GO)
Biological_process	DNA integration Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW recombinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

183

Transposon gamma-delta resolvase

PRO_0000196367

Regions

DNA binding

20

H-T-H motif Potential

Sites

Active site

1

O-(5'-phospho-DNA)-serine intermediate Ref.5

Secondary structure

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183

Helix Strand Turn

Sequences

Sequence

Length

Mass (Da)

Tools

P03012 [UniParc].

Last modified July 21, 1986. Version 1.
Checksum: B2EBA6037F926FAB
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183

20,362

        10         20         30         40         50         60 
MRLFGYARVS TSQQSLDIQV RALKDAGVKA NRIFTDKASG SSSDRKGLDL LRMKVEEGDV 

        70         80         90        100        110        120 
ILVKKLDRLG RDTADMIQLI KEFDAQGVSI RFIDDGISTD GEMGKMVVTI LSAVAQAERQ 

       130        140        150        160        170        180 
RILERTNEGR QEAMAKGVVF GRKRKIDRDA VLNMWQQGLG ASHISKTMNI ARSTVYKVIN 


ESN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of gamma delta resolvase gene and demonstration that its gene product acts as a repressor of transcription." Reed R.R., Shibuya G.I., Steitz J.A. Nature 300:381-383(1982) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Identification of the region that determines the specificity of binding of the transposases encoded by Tn3 and gamma delta to the terminal inverted repeat sequences." Maekawa T., Ohtsubo E. Jpn. J. Genet. 69:269-285(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Sequence of a transposon identified as Tn1000 (gamma delta)." Broom J.E., Hill D.F., Hughes G., Jones W.A., McNaughton J.C., Stockwell P.A., Petersen G.B. DNA Seq. 5:185-189(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Complete nucleotide sequence of the F plasmid: its implications for organization and diversification of plasmid genomes." Shimizu H., Saitoh Y., Suda Y., Uehara K., Sampei G., Mizobuchi K. Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / CR63.
[5]	"Resolvase-mediated recombination intermediates contain a serine residue covalently linked to DNA." Reed R.R., Moser C.D. Cold Spring Harb. Symp. Quant. Biol. 49:245-249(1984) [PubMed] [Europe PMC] [Abstract] Cited for: ACTIVE SITE SER-10.
[6]	"Mutants of the gamma delta resolvase: a genetic analysis of the recombination function." Newman B.J., Grindley N.D.F. Cell 38:463-469(1984) [PubMed] [Europe PMC] [Abstract] Cited for: MUTAGENESIS.
[7]	"The crystal structure of the catalytic domain of the site-specific recombination enzyme gamma delta resolvase at 2.7-A resolution." Sanderson M.R., Freemont P.S., Rice P.A., Goldman A., Hatfull G.F., Grindley N.D.F., Steitz T.A. Cell 63:1323-1329(1990) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 1-140.
[8]	"Crystal structure of the site-specific recombinase gamma delta resolvase complexed with a 34 bp cleavage site." Yang W., Steitz T.A. Cell 82:193-207(1995) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS).
[9]	"Determination of the structure of the DNA binding domain of gamma delta resolvase in solution." Liu T., Derose E.F., Mullen G.P. Protein Sci. 3:1286-1295(1994) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR OF 141-183.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

J01844 Genomic DNA. No translation available.
X60200 Genomic DNA. Translation: CAA42759.1.
D16449 Genomic DNA. Translation: BAA03915.1.
AP001918 Genomic DNA. Translation: BAA97879.1.

PIR

RPECTG. A03542.

RefSeq

NP_061388.1. NC_002483.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1GDR	X-ray	3.50	A	1-140	[»]
1GDT	X-ray	3.00	A/B	1-183	[»]
1GHT	NMR	-	A	1-105	[»]
1HX7	NMR	-	A	1-105	[»]
1RES	NMR	-	A	141-183	[»]
1RET	NMR	-	A	141-183	[»]
1ZR2	X-ray	3.90	A/B	3-183	[»]
1ZR4	X-ray	3.40	A/B/D/E	3-183	[»]
2GM4	X-ray	3.50	A/B	3-183	[»]
2GM5	X-ray	2.10	A/B/C/D	3-134	[»]
2RSL	X-ray	2.30	A/B/C	1-140	[»]

ProteinModelPortal

SMR

P03012. Positions 1-183.

ModBase

Proteomic databases

PRIDE

Protocols and materials databases

StructuralBiologyKnowledgebase

Genome annotation databases

GeneID

Phylogenomic databases

HOGENOM

OMA

ProtClustDB

Gene expression databases

Genevestigator

Family and domain databases

Gene3D

1.10.10.60. 1 hit.
3.40.50.1390. 1 hit.

InterPro

IPR009057. Homeodomain-like.
IPR006118. Recombinase_CS.
IPR006119. Resolv_N.
IPR006120. Resolvase_HTH_dom.
[Graphical view]

Pfam

PF02796. HTH_7. 1 hit.
PF00239. Resolvase. 1 hit.
[Graphical view]

SMART

SM00857. Resolvase. 1 hit.
[Graphical view]

SUPFAM

SSF46689. Homeodomain_like. 1 hit.
SSF53041. Resolv_N. 1 hit.

PROSITE

PS00397. RECOMBINASES_1. 1 hit.
PS00398. RECOMBINASES_2. 1 hit.
[Graphical view]

ProtoNet

Other

EvolutionaryTrace

Entry information

Entry name

TNR1_ECOLI

Accession

Primary (citable) accession number: P03012

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	May 1, 2013
This is version 108 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents