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Protein

Transcription factor IIIA

Gene

gtf3a

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as both a positive transcription factor for 5S RNA genes and a specific RNA binding protein that complexes with 5S RNA in oocytes to form the 7S ribonucleoprotein storage particle. May play an essential role in the developmental change in 5S RNA gene expression. Interacts with the internal control region (ICR) of approximately 50 bases within the 5S RNA genes, is required for correct transcription of these genes by RNA polymerase III. Also binds the transcribed 5S RNA's.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri35 – 59C2H2-type 1PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri65 – 89C2H2-type 2PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri95 – 120C2H2-type 3PROSITE-ProRule annotationAdd BLAST26
Zinc fingeri127 – 151C2H2-type 4PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri157 – 181C2H2-type 5PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri184 – 210C2H2-type 6PROSITE-ProRule annotationAdd BLAST27
Zinc fingeri214 – 236C2H2-type 7PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri243 – 268C2H2-type 8PROSITE-ProRule annotationAdd BLAST26
Zinc fingeri274 – 298C2H2-type 9PROSITE-ProRule annotationAdd BLAST25

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, RNA-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor IIIA
Short name:
TFIIIA
Alternative name(s):
S-TFIIIA/O-TFIIIA
Gene namesi
Name:gtf3a
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-6252591. gtf3a.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi38S → E or A: Abolishes phosphorylation by CK2. 1 Publication1
Mutagenesisi42C → S: Loss of DNA binding. 1 Publication1
Mutagenesisi55H → L: Inhibition of specific DNA binding. 1 Publication1
Mutagenesisi91G → S: Loss of DNA binding. 1 Publication1
Mutagenesisi93K → E: Loss of DNA binding. 1 Publication1
Mutagenesisi134C → S: No effect. 1 Publication1
Mutagenesisi186C → S: No effect. 1 Publication1
Mutagenesisi336S → E or A: Slightly decreases phosphorylation by CK2. 1 Publication1
Mutagenesisi350S → E or A: No effect on phosphorylation by CK2. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000416321 – 366Transcription factor IIIAAdd BLAST366

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei38Phosphoserine; by CK21 Publication1
Modified residuei336Phosphoserine; by CK2; in vitro1 Publication1

Post-translational modificationi

The N-terminus is blocked.

Keywords - PTMi

Phosphoprotein

PTM databases

iPTMnetiP03001.

Expressioni

Tissue specificityi

Synthesized in oocytes and, in much lower levels, in somatic cells.

Developmental stagei

The levels follow the transcriptional activity of oocyte type 5S RNA genes during embryogenesis, present in very high levels in maturing oocytes when oocyte type 5S genes are being expressed, and in much lower levels in somatic cells where the oocyte type genes are not expressed.

Structurei

Secondary structure

1366
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi38 – 41Combined sources4
Beta strandi45 – 48Combined sources4
Helixi49 – 60Combined sources12
Beta strandi64 – 66Combined sources3
Beta strandi75 – 78Combined sources4
Helixi79 – 86Combined sources8
Turni87 – 91Combined sources5
Beta strandi99 – 101Combined sources3
Beta strandi105 – 107Combined sources3
Helixi109 – 117Combined sources9
Turni122 – 124Combined sources3
Beta strandi131 – 134Combined sources4
Beta strandi137 – 140Combined sources4
Helixi141 – 152Combined sources12
Beta strandi156 – 158Combined sources3
Beta strandi161 – 163Combined sources3
Beta strandi167 – 170Combined sources4
Helixi171 – 182Combined sources12
Beta strandi183 – 185Combined sources3
Turni189 – 191Combined sources3
Beta strandi195 – 198Combined sources4
Helixi199 – 208Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1TF3NMR-A32-123[»]
1TF6X-ray3.10A/D23-212[»]
1UN6X-ray3.10B/C/D127-212[»]
2HGHNMR-A127-212[»]
2J7JX-ray1.65A127-210[»]
ProteinModelPortaliP03001.
SMRiP03001.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP03001.

Family & Domainsi

Sequence similaritiesi

Contains 9 C2H2-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri35 – 59C2H2-type 1PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri65 – 89C2H2-type 2PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri95 – 120C2H2-type 3PROSITE-ProRule annotationAdd BLAST26
Zinc fingeri127 – 151C2H2-type 4PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri157 – 181C2H2-type 5PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri184 – 210C2H2-type 6PROSITE-ProRule annotationAdd BLAST27
Zinc fingeri214 – 236C2H2-type 7PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri243 – 268C2H2-type 8PROSITE-ProRule annotationAdd BLAST26
Zinc fingeri274 – 298C2H2-type 9PROSITE-ProRule annotationAdd BLAST25

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

HOVERGENiHBG017915.
KOiK09191.

Family and domain databases

Gene3Di3.30.160.60. 9 hits.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 9 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 8 hits.
PS50157. ZINC_FINGER_C2H2_2. 7 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform Somatic (identifier: P03001-1) [UniParc]FASTAAdd to basket
Also known as: S-TFIIIA

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAKVASTSS EEAEGSLVTE GEMGEKALPV VYKRYICSFA DCGAAYNKNW
60 70 80 90 100
KLQAHLCKHT GEKPFPCKEE GCEKGFTSLH HLTRHSLTHT GEKNFTCDSD
110 120 130 140 150
GCDLRFTTKA NMKKHFNRFH NIKICVYVCH FENCGKAFKK HNQLKVHQFS
160 170 180 190 200
HTQQLPYECP HEGCDKRFSL PSRLKRHEKV HAGYPCKKDD SCSFVGKTWT
210 220 230 240 250
LYLKHVAECH QDLAVCDVCN RKFRHKDYLR DHQKTHEKER TVYLCPRDGC
260 270 280 290 300
DRSYTTAFNL RSHIQSFHEE QRPFVCEHAG CGKCFAMKKS LERHSVVHDP
310 320 330 340 350
EKRKLKEKCP RPKRSLASRL TGYIPPKSKE KNASVSGTEK TDSLVKNKPS
360
GTETNGSLVL DKLTIQ
Length:366
Mass (Da):41,911
Last modified:July 15, 1998 - v2
Checksum:i29237E710292FA24
GO
Isoform Oocyte (identifier: P03001-2) [UniParc]FASTAAdd to basket
Also known as: O-TFIIIA

The sequence of this isoform differs from the canonical sequence as follows:
     1-22: Missing.

Show »
Length:344
Mass (Da):39,746
Checksum:i8ACFE717EA8A1322
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti4K → T in CAA33786 (PubMed:2744458).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti96T → K.1
Natural varianti319R → C.1
Natural varianti335V → I.1
Natural varianti356G → D.1
Natural varianti365I → L.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0189621 – 22Missing in isoform Oocyte. CuratedAdd BLAST22

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K02938 mRNA. Translation: AAA49967.1.
X03681
, X03735, X03736, X03737, X03738, X03739 Genomic DNA. Translation: CAB51745.1.
X15785 Genomic DNA. Translation: CAA33786.1.
PIRiA35916.
A90857. TWXL3.
RefSeqiNP_001081328.1. NM_001087859.1. [P03001-2]
UniGeneiXl.860.

Genome annotation databases

GeneIDi397777.
KEGGixla:397777.

Keywords - Coding sequence diversityi

Alternative initiation, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K02938 mRNA. Translation: AAA49967.1.
X03681
, X03735, X03736, X03737, X03738, X03739 Genomic DNA. Translation: CAB51745.1.
X15785 Genomic DNA. Translation: CAA33786.1.
PIRiA35916.
A90857. TWXL3.
RefSeqiNP_001081328.1. NM_001087859.1. [P03001-2]
UniGeneiXl.860.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1TF3NMR-A32-123[»]
1TF6X-ray3.10A/D23-212[»]
1UN6X-ray3.10B/C/D127-212[»]
2HGHNMR-A127-212[»]
2J7JX-ray1.65A127-210[»]
ProteinModelPortaliP03001.
SMRiP03001.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

iPTMnetiP03001.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi397777.
KEGGixla:397777.

Organism-specific databases

CTDi2971.
XenbaseiXB-GENE-6252591. gtf3a.

Phylogenomic databases

HOVERGENiHBG017915.
KOiK09191.

Miscellaneous databases

EvolutionaryTraceiP03001.

Family and domain databases

Gene3Di3.30.160.60. 9 hits.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 9 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 8 hits.
PS50157. ZINC_FINGER_C2H2_2. 7 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTF3A_XENLA
AccessioniPrimary (citable) accession number: P03001
Secondary accession number(s): Q91856
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 15, 1998
Last modified: November 2, 2016
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.