P03000 (IF3_GEOSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Translation initiation factor IF-3

Gene names

Name:

infC

Organism

Geobacillus stearothermophilus (Bacillus stearothermophilus)

Taxonomic identifier

1422 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Geobacillus

Protein attributes

Sequence length	172 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	IF-3 binds to the 30S ribosomal subunit and shifts the equilibrum between 70S ribosomes and their 50S and 30S subunits in favor of the free subunits, thus enhancing the availability of 30S subunits on which protein synthesis initiation begins. HAMAP MF_00080
Subunit structure	Monomer.
Subcellular location	Cytoplasm HAMAP MF_00080.
Sequence similarities	Belongs to the IF-3 family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Molecular function	Initiation factor
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	translation initiation factor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.2

Chain

2 – 172

171

Translation initiation factor IF-3 HAMAP MF_00080

PRO_0000177481

Secondary structure

1

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172

Helix Strand Turn

Details...

Helix

8 – 10

3

Beta strand

14 – 19

6

Beta strand

25 – 30

6

Helix

31 – 40

10

Beta strand

44 – 49

6

Beta strand

52 – 54

3

Beta strand

56 – 60

5

Helix

62 – 75

14

Beta strand

85 – 90

6

Helix

96 – 111

16

Beta strand

115 – 121

7

Helix

129 – 143

15

Turn

144 – 147

4

Beta strand

148 – 157

10

Beta strand

160 – 168

9

Sequences

	Sequence		Length	Mass (Da)
	P03000 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 379902B302ECD929	FASTA	172	19,809
	10 20 30 40 50 60 MSKDFIINEQ IRAREVRLID QNGDQLGIKS KQEALEIAAR RNLDLVLVAP NAKPPVCRIM 70 80 90 100 110 120 DYGKFRFEQQ KKEKEARKKQ KVINVKEVRL SPTIEEHDFN TKLRNARKFL EKGDKVKATI 130 140 150 160 170 RFKGRAITHK EIGQRVLDRL SEACADIAVV ETAPKMDGRN MFLVLAPKND NK

References

[1]	"Cloning and characterization of a gene cluster from Bacillus stearothermophilus comprising infC, rpmI and rplT." Pon C.L., Brombach M., Thamm S., Gualerzi C.O. Mol. Gen. Genet. 218:355-357(1989) [PubMed: 2779520] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The primary structure of initiation factor IF3 from Bacillus stearothermophilus." Kimura M., Ernst H., Appelt K. FEBS Lett. 160:78-81(1983) [PubMed: 6884517] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-172. Strain: ATCC 29609 / DSM 2027 / NCA 1503 / NCIMB 8924.
[3]	"Site-directed mutagenesis and NMR spectroscopic approaches to the elucidation of the structure-function relationships in translation initiation factors IF1 and IF3." Spurio R., Paci M., Pawlik R.T., la Teana A., Digiacco B.V., Pon C.L., Gualerzi C.O. Biochimie 73:1001-1006(1991) [PubMed: 1742345] [Abstract] Cited for: STRUCTURE BY NMR, MUTAGENESIS.
[4]	"On the global architecture of initiation factor IF3: a comparative study of the linker regions from the Escherichia coli protein and the Bacillus stearothermophilus protein." Hua Y., Raleigh D.P. J. Mol. Biol. 278:871-878(1998) [PubMed: 9614948] [Abstract] Cited for: STRUCTURE BY NMR.
[5]	"X-ray crystallography shows that translational initiation factor IF3 consists of two compact alpha/beta domains linked by an alpha-helix." Biou V., Shu F., Ramakrishnan V. EMBO J. 14:4056-4064(1995) [PubMed: 7664745] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X16188 Genomic DNA. Translation: CAA34312.1.

PIR

FIBS3F. S05346.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1TIF	X-ray	1.80	A	1-78	[»]
1TIG	X-ray	2.00	A	79-172	[»]

ProteinModelPortal

P03000.

SMR

P03000. Positions 3-78, 83-170.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

HAMAP

MF_00080. IF-3.
[Tree]

InterPro

IPR019813. Translation_initiation_fac3_CS.
IPR001288. Translation_initiation_fac_3.
IPR019815. Translation_initiation_fac_3_C.
IPR019814. Translation_initiation_fac_3_N.
[Graphical view]

Gene3D

G3DSA:3.10.20.80. IF3. 1 hit.
G3DSA:3.30.110.10. IF3. 1 hit.

PANTHER

PTHR10938. IF3. 1 hit.

Pfam

PF00707. IF3_C. 1 hit.
PF05198. IF3_N. 1 hit.
[Graphical view]

SUPFAM

SSF54364. IF3. 1 hit.
SSF55200. IF3. 1 hit.

TIGRFAMs

TIGR00168. InfC. 1 hit.

PROSITE

PS00938. IF3. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

IF3_GEOSE

Accession

Primary (citable) accession number: P03000

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	January 23, 2007
Last modified:	May 31, 2011
This is version 75 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program