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Protein

Elongation factor Tu, mitochondrial

Gene

TUF1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

G-protein that, in its active GTP-bound form, binds to and delivers aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. In the presence of a correct codon-anticodon match between the aminoacyl-tRNA and the A-site codon of the ribosome-bound mRNA, the ribosome acts as a GTPase activator and the GTP is hydrolyzed. The inactive GDP-bound form leaves the ribosome and must be recycled before binding another molecule of aminoacyl-tRNA. Required for mitochondrial protein biosynthesis and maintenance of mitochondrial DNA.1 Publication

Pathway:ipolypeptide chain elongation

This protein is involved in the pathway polypeptide chain elongation, which is part of Protein biosynthesis.
View all proteins of this organism that are known to be involved in the pathway polypeptide chain elongation and in Protein biosynthesis.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi55 – 628GTPBy similarity
Nucleotide bindingi117 – 1215GTPBy similarity
Nucleotide bindingi172 – 1754GTPBy similarity

GO - Molecular functioni

  • GTPase activity Source: SGD
  • GTP binding Source: UniProtKB-KW
  • translation elongation factor activity Source: SGD

GO - Biological processi

  • mitochondrial translation Source: SGD
  • mitochondrial translational elongation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Elongation factor

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33697-MONOMER.
UniPathwayiUPA00345.

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor Tu, mitochondrial
Alternative name(s):
tufM
Gene namesi
Name:TUF1
Synonyms:TUFM
Ordered Locus Names:YOR187W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome XV

Organism-specific databases

CYGDiYOR187w.
EuPathDBiFungiDB:YOR187W.
SGDiS000005713. TUF1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3838MitochondrionCuratedAdd
BLAST
Chaini39 – 437399Elongation factor Tu, mitochondrialPRO_0000007465Add
BLAST

Post-translational modificationi

The precursor is processed in two steps involving mitochondrial intermediate peptidase (MIP) and mitochondrial processing peptidase (MPP).1 Publication

Proteomic databases

MaxQBiP02992.
PaxDbiP02992.
PeptideAtlasiP02992.
PRIDEiP02992.

Interactioni

Protein-protein interaction databases

BioGridi34582. 67 interactions.
DIPiDIP-4130N.
IntActiP02992. 12 interactions.
MINTiMINT-497322.

Structurei

3D structure databases

ProteinModelPortaliP02992.
SMRiP02992. Positions 45-437.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini46 – 242197tr-type GAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni55 – 628G1By similarity
Regioni96 – 1005G2By similarity
Regioni117 – 1204G3By similarity
Regioni172 – 1754G4By similarity
Regioni210 – 2123G5By similarity

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0050.
GeneTreeiENSGT00550000074682.
HOGENOMiHOG000229290.
InParanoidiP02992.
KOiK02358.
OMAiGMVICKP.
OrthoDBiEOG7GXPMZ.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00118_B. EF_Tu_B.
InterProiIPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
IPR004161. Transl_elong_EFTu/EF1A_2.
IPR004541. Transl_elong_EFTu/EF1A_bac/org.
IPR004160. Transl_elong_EFTu/EF1A_C.
[Graphical view]
PfamiPF03144. GTP_EFTU_D2. 1 hit.
PF03143. GTP_EFTU_D3. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00485. EF-Tu. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P02992-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSALLPRLLT RTAFKASGKL LRLSSVISRT FSQTTTSYAA AFDRSKPHVN
60 70 80 90 100
IGTIGHVDHG KTTLTAAITK TLAAKGGANF LDYAAIDKAP EERARGITIS
110 120 130 140 150
TAHVEYETAK RHYSHVDCPG HADYIKNMIT GAAQMDGAII VVAATDGQMP
160 170 180 190 200
QTREHLLLAR QVGVQHIVVF VNKVDTIDDP EMLELVEMEM RELLNEYGFD
210 220 230 240 250
GDNAPIIMGS ALCALEGRQP EIGEQAIMKL LDAVDEYIPT PERDLNKPFL
260 270 280 290 300
MPVEDIFSIS GRGTVVTGRV ERGNLKKGEE LEIVGHNSTP LKTTVTGIEM
310 320 330 340 350
FRKELDSAMA GDNAGVLLRG IRRDQLKRGM VLAKPGTVKA HTKILASLYI
360 370 380 390 400
LSKEEGGRHS GFGENYRPQM FIRTADVTVV MRFPKEVEDH SMQVMPGDNV
410 420 430
EMECDLIHPT PLEVGQRFNI REGGRTVGTG LITRIIE
Length:437
Mass (Da):47,972
Last modified:July 21, 1986 - v1
Checksum:i26F4DFE805D1A93B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K00428 Genomic DNA. No translation available.
Z75095 Genomic DNA. Translation: CAA99396.1.
BK006948 Genomic DNA. Translation: DAA10959.1.
PIRiA03520. EFBYT.
RefSeqiNP_014830.1. NM_001183606.1.

Genome annotation databases

EnsemblFungiiYOR187W; YOR187W; YOR187W.
GeneIDi854359.
KEGGisce:YOR187W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K00428 Genomic DNA. No translation available.
Z75095 Genomic DNA. Translation: CAA99396.1.
BK006948 Genomic DNA. Translation: DAA10959.1.
PIRiA03520. EFBYT.
RefSeqiNP_014830.1. NM_001183606.1.

3D structure databases

ProteinModelPortaliP02992.
SMRiP02992. Positions 45-437.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34582. 67 interactions.
DIPiDIP-4130N.
IntActiP02992. 12 interactions.
MINTiMINT-497322.

Proteomic databases

MaxQBiP02992.
PaxDbiP02992.
PeptideAtlasiP02992.
PRIDEiP02992.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR187W; YOR187W; YOR187W.
GeneIDi854359.
KEGGisce:YOR187W.

Organism-specific databases

CYGDiYOR187w.
EuPathDBiFungiDB:YOR187W.
SGDiS000005713. TUF1.

Phylogenomic databases

eggNOGiCOG0050.
GeneTreeiENSGT00550000074682.
HOGENOMiHOG000229290.
InParanoidiP02992.
KOiK02358.
OMAiGMVICKP.
OrthoDBiEOG7GXPMZ.

Enzyme and pathway databases

UniPathwayiUPA00345.
BioCyciYEAST:G3O-33697-MONOMER.

Miscellaneous databases

NextBioi976464.
PROiP02992.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00118_B. EF_Tu_B.
InterProiIPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
IPR004161. Transl_elong_EFTu/EF1A_2.
IPR004541. Transl_elong_EFTu/EF1A_bac/org.
IPR004160. Transl_elong_EFTu/EF1A_C.
[Graphical view]
PfamiPF03144. GTP_EFTU_D2. 1 hit.
PF03143. GTP_EFTU_D3. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00485. EF-Tu. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and sequence determination of the nuclear gene coding for mitochondrial elongation factor Tu of Saccharomyces cerevisiae."
    Nagata S., Tsunetsugu-Yokota Y., Naito A., Kaziro Y.
    Proc. Natl. Acad. Sci. U.S.A. 80:6192-6196(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
    Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
    , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
    Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Mitochondrial protein synthesis is required for maintenance of intact mitochondrial genomes in Saccharomyces cerevisiae."
    Myers A.M., Pape L.K., Tzagoloff A.
    EMBO J. 4:2087-2092(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "Prediction and identification of new natural substrates of the yeast mitochondrial intermediate peptidase."
    Branda S.S., Isaya G.
    J. Biol. Chem. 270:27366-27373(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: CLEAVAGE BY MPP AND MIP, IDENTIFICATION OF PROBABLE CLEAVAGE SITE.
  6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    Strain: ATCC 76625 / YPH499.
  9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiEFTU_YEAST
AccessioniPrimary (citable) accession number: P02992
Secondary accession number(s): D6W2P3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: July 22, 2015
This is version 143 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 58527 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.