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P02992

- EFTU_YEAST

UniProt

P02992 - EFTU_YEAST

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Protein

Elongation factor Tu, mitochondrial

Gene
TUF1, TUFM, YOR187W
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

G-protein that, in its active GTP-bound form, binds to and delivers aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. In the presence of a correct codon-anticodon match between the aminoacyl-tRNA and the A-site codon of the ribosome-bound mRNA, the ribosome acts as a GTPase activator and the GTP is hydrolyzed. The inactive GDP-bound form leaves the ribosome and must be recycled before binding another molecule of aminoacyl-tRNA. Required for mitochondrial protein biosynthesis and maintenance of mitochondrial DNA.1 Publication

Pathwayi

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi55 – 628GTP By similarity
Nucleotide bindingi117 – 1215GTP By similarity
Nucleotide bindingi172 – 1754GTP By similarity

GO - Molecular functioni

  1. GTPase activity Source: SGD
  2. GTP binding Source: UniProtKB-KW
  3. translation elongation factor activity Source: SGD

GO - Biological processi

  1. GTP catabolic process Source: GOC
  2. mitochondrial translation Source: SGD
  3. mitochondrial translational elongation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Elongation factor

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33697-MONOMER.
UniPathwayiUPA00345.

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor Tu, mitochondrial
Alternative name(s):
tufM
Gene namesi
Name:TUF1
Synonyms:TUFM
Ordered Locus Names:YOR187W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XV

Organism-specific databases

CYGDiYOR187w.
SGDiS000005713. TUF1.

Subcellular locationi

Mitochondrion 2 Publications

GO - Cellular componenti

  1. mitochondrial matrix Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3838Mitochondrion InferredAdd
BLAST
Chaini39 – 437399Elongation factor Tu, mitochondrialUniRule annotationPRO_0000007465Add
BLAST

Post-translational modificationi

The precursor is processed in two steps involving mitochondrial intermediate peptidase (MIP) and mitochondrial processing peptidase (MPP).UniRule annotation

Proteomic databases

MaxQBiP02992.
PaxDbiP02992.
PeptideAtlasiP02992.
PRIDEiP02992.

Expressioni

Gene expression databases

GenevestigatoriP02992.

Interactioni

Protein-protein interaction databases

BioGridi34582. 68 interactions.
DIPiDIP-4130N.
IntActiP02992. 12 interactions.
MINTiMINT-497322.
STRINGi4932.YOR187W.

Structurei

3D structure databases

ProteinModelPortaliP02992.
SMRiP02992. Positions 45-437.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini46 – 242197tr-type GAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni55 – 628G1 By similarity
Regioni96 – 1005G2 By similarity
Regioni117 – 1204G3 By similarity
Regioni172 – 1754G4 By similarity
Regioni210 – 2123G5 By similarity

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0050.
GeneTreeiENSGT00550000074682.
HOGENOMiHOG000229290.
KOiK02358.
OMAiGTEMCMP.
OrthoDBiEOG7GXPMZ.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00118_B. EF_Tu_B.
InterProiIPR000795. EF_GTP-bd_dom.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
IPR004161. Transl_elong_EFTu/EF1A_2.
IPR004541. Transl_elong_EFTu/EF1A_bac/org.
IPR004160. Transl_elong_EFTu/EF1A_C.
[Graphical view]
PfamiPF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF03143. GTP_EFTU_D3. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00485. EF-Tu. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P02992-1 [UniParc]FASTAAdd to Basket

« Hide

MSALLPRLLT RTAFKASGKL LRLSSVISRT FSQTTTSYAA AFDRSKPHVN    50
IGTIGHVDHG KTTLTAAITK TLAAKGGANF LDYAAIDKAP EERARGITIS 100
TAHVEYETAK RHYSHVDCPG HADYIKNMIT GAAQMDGAII VVAATDGQMP 150
QTREHLLLAR QVGVQHIVVF VNKVDTIDDP EMLELVEMEM RELLNEYGFD 200
GDNAPIIMGS ALCALEGRQP EIGEQAIMKL LDAVDEYIPT PERDLNKPFL 250
MPVEDIFSIS GRGTVVTGRV ERGNLKKGEE LEIVGHNSTP LKTTVTGIEM 300
FRKELDSAMA GDNAGVLLRG IRRDQLKRGM VLAKPGTVKA HTKILASLYI 350
LSKEEGGRHS GFGENYRPQM FIRTADVTVV MRFPKEVEDH SMQVMPGDNV 400
EMECDLIHPT PLEVGQRFNI REGGRTVGTG LITRIIE 437
Length:437
Mass (Da):47,972
Last modified:July 21, 1986 - v1
Checksum:i26F4DFE805D1A93B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
K00428 Genomic DNA. No translation available.
Z75095 Genomic DNA. Translation: CAA99396.1.
BK006948 Genomic DNA. Translation: DAA10959.1.
PIRiA03520. EFBYT.
RefSeqiNP_014830.1. NM_001183606.1.

Genome annotation databases

EnsemblFungiiYOR187W; YOR187W; YOR187W.
GeneIDi854359.
KEGGisce:YOR187W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
K00428 Genomic DNA. No translation available.
Z75095 Genomic DNA. Translation: CAA99396.1 .
BK006948 Genomic DNA. Translation: DAA10959.1 .
PIRi A03520. EFBYT.
RefSeqi NP_014830.1. NM_001183606.1.

3D structure databases

ProteinModelPortali P02992.
SMRi P02992. Positions 45-437.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 34582. 68 interactions.
DIPi DIP-4130N.
IntActi P02992. 12 interactions.
MINTi MINT-497322.
STRINGi 4932.YOR187W.

Proteomic databases

MaxQBi P02992.
PaxDbi P02992.
PeptideAtlasi P02992.
PRIDEi P02992.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YOR187W ; YOR187W ; YOR187W .
GeneIDi 854359.
KEGGi sce:YOR187W.

Organism-specific databases

CYGDi YOR187w.
SGDi S000005713. TUF1.

Phylogenomic databases

eggNOGi COG0050.
GeneTreei ENSGT00550000074682.
HOGENOMi HOG000229290.
KOi K02358.
OMAi GTEMCMP.
OrthoDBi EOG7GXPMZ.

Enzyme and pathway databases

UniPathwayi UPA00345 .
BioCyci YEAST:G3O-33697-MONOMER.

Miscellaneous databases

NextBioi 976464.
PROi P02992.

Gene expression databases

Genevestigatori P02992.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
HAMAPi MF_00118_B. EF_Tu_B.
InterProi IPR000795. EF_GTP-bd_dom.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
IPR004161. Transl_elong_EFTu/EF1A_2.
IPR004541. Transl_elong_EFTu/EF1A_bac/org.
IPR004160. Transl_elong_EFTu/EF1A_C.
[Graphical view ]
Pfami PF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF03143. GTP_EFTU_D3. 1 hit.
[Graphical view ]
PRINTSi PR00315. ELONGATNFCT.
SUPFAMi SSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsi TIGR00485. EF-Tu. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEi PS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and sequence determination of the nuclear gene coding for mitochondrial elongation factor Tu of Saccharomyces cerevisiae."
    Nagata S., Tsunetsugu-Yokota Y., Naito A., Kaziro Y.
    Proc. Natl. Acad. Sci. U.S.A. 80:6192-6196(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
    Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
    , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
    Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Mitochondrial protein synthesis is required for maintenance of intact mitochondrial genomes in Saccharomyces cerevisiae."
    Myers A.M., Pape L.K., Tzagoloff A.
    EMBO J. 4:2087-2092(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "Prediction and identification of new natural substrates of the yeast mitochondrial intermediate peptidase."
    Branda S.S., Isaya G.
    J. Biol. Chem. 270:27366-27373(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: CLEAVAGE BY MPP AND MIP, IDENTIFICATION OF PROBABLE CLEAVAGE SITE.
  6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    Strain: ATCC 76625 / YPH499.
  9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiEFTU_YEAST
AccessioniPrimary (citable) accession number: P02992
Secondary accession number(s): D6W2P3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: September 3, 2014
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 58527 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

External Data

Dasty 3

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