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Protein

Elongation factor Tu, mitochondrial

Gene

TUF1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

G-protein that, in its active GTP-bound form, binds to and delivers aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. In the presence of a correct codon-anticodon match between the aminoacyl-tRNA and the A-site codon of the ribosome-bound mRNA, the ribosome acts as a GTPase activator and the GTP is hydrolyzed. The inactive GDP-bound form leaves the ribosome and must be recycled before binding another molecule of aminoacyl-tRNA. Required for mitochondrial protein biosynthesis and maintenance of mitochondrial DNA.1 Publication

Miscellaneous

Present with 58527 molecules/cell in log phase SD medium.1 Publication

Pathwayi: polypeptide chain elongation

This protein is involved in the pathway polypeptide chain elongation, which is part of Protein biosynthesis.
View all proteins of this organism that are known to be involved in the pathway polypeptide chain elongation and in Protein biosynthesis.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi55 – 62GTPBy similarity8
Nucleotide bindingi117 – 121GTPBy similarity5
Nucleotide bindingi172 – 175GTPBy similarity4

GO - Molecular functioni

  • GTPase activity Source: SGD
  • GTP binding Source: UniProtKB-KW
  • translation elongation factor activity Source: SGD

GO - Biological processi

  • mitochondrial translation Source: SGD
  • mitochondrial translational elongation Source: SGD

Keywordsi

Molecular functionElongation factor
Biological processProtein biosynthesis
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33697-MONOMER
UniPathwayiUPA00345

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor Tu, mitochondrial
Alternative name(s):
tufM
Gene namesi
Name:TUF1
Synonyms:TUFM
Ordered Locus Names:YOR187W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi

Organism-specific databases

EuPathDBiFungiDB:YOR187W
SGDiS000005713 TUF1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 38MitochondrionCuratedAdd BLAST38
ChainiPRO_000000746539 – 437Elongation factor Tu, mitochondrialAdd BLAST399

Post-translational modificationi

The precursor is processed in two steps involving mitochondrial intermediate peptidase (MIP) and mitochondrial processing peptidase (MPP).1 Publication

Proteomic databases

MaxQBiP02992
PaxDbiP02992
PRIDEiP02992

PTM databases

iPTMnetiP02992

Interactioni

Protein-protein interaction databases

BioGridi34582, 187 interactors
DIPiDIP-4130N
IntActiP02992, 14 interactors
MINTiP02992
STRINGi4932.YOR187W

Structurei

3D structure databases

ProteinModelPortaliP02992
SMRiP02992
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini46 – 242tr-type GAdd BLAST197

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni55 – 62G1By similarity8
Regioni96 – 100G2By similarity5
Regioni117 – 120G3By similarity4
Regioni172 – 175G4By similarity4
Regioni210 – 212G5By similarity3

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

GeneTreeiENSGT00550000074682
HOGENOMiHOG000229290
InParanoidiP02992
KOiK02358
OMAiYGHIDCP
OrthoDBiEOG092C2HV8

Family and domain databases

CDDicd03697 EFTU_II, 1 hit
HAMAPiMF_00118_B EF_Tu_B, 1 hit
InterProiView protein in InterPro
IPR004161 EFTu-like_2
IPR033720 EFTU_2
IPR031157 G_TR_CS
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR000795 TF_GTP-bd_dom
IPR009000 Transl_B-barrel_sf
IPR009001 Transl_elong_EF1A/Init_IF2_C
IPR004541 Transl_elong_EFTu/EF1A_bac/org
IPR004160 Transl_elong_EFTu/EF1A_C
PfamiView protein in Pfam
PF00009 GTP_EFTU, 1 hit
PF03144 GTP_EFTU_D2, 1 hit
PF03143 GTP_EFTU_D3, 1 hit
PRINTSiPR00315 ELONGATNFCT
SUPFAMiSSF50447 SSF50447, 1 hit
SSF50465 SSF50465, 1 hit
SSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00485 EF-Tu, 1 hit
TIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS00301 G_TR_1, 1 hit
PS51722 G_TR_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P02992-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSALLPRLLT RTAFKASGKL LRLSSVISRT FSQTTTSYAA AFDRSKPHVN
60 70 80 90 100
IGTIGHVDHG KTTLTAAITK TLAAKGGANF LDYAAIDKAP EERARGITIS
110 120 130 140 150
TAHVEYETAK RHYSHVDCPG HADYIKNMIT GAAQMDGAII VVAATDGQMP
160 170 180 190 200
QTREHLLLAR QVGVQHIVVF VNKVDTIDDP EMLELVEMEM RELLNEYGFD
210 220 230 240 250
GDNAPIIMGS ALCALEGRQP EIGEQAIMKL LDAVDEYIPT PERDLNKPFL
260 270 280 290 300
MPVEDIFSIS GRGTVVTGRV ERGNLKKGEE LEIVGHNSTP LKTTVTGIEM
310 320 330 340 350
FRKELDSAMA GDNAGVLLRG IRRDQLKRGM VLAKPGTVKA HTKILASLYI
360 370 380 390 400
LSKEEGGRHS GFGENYRPQM FIRTADVTVV MRFPKEVEDH SMQVMPGDNV
410 420 430
EMECDLIHPT PLEVGQRFNI REGGRTVGTG LITRIIE
Length:437
Mass (Da):47,972
Last modified:July 21, 1986 - v1
Checksum:i26F4DFE805D1A93B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K00428 Genomic DNA No translation available.
Z75095 Genomic DNA Translation: CAA99396.1
BK006948 Genomic DNA Translation: DAA10959.1
PIRiA03520 EFBYT
RefSeqiNP_014830.1, NM_001183606.1

Genome annotation databases

EnsemblFungiiYOR187W; YOR187W; YOR187W
GeneIDi854359
KEGGisce:YOR187W

Similar proteinsi

Entry informationi

Entry nameiEFTU_YEAST
AccessioniPrimary (citable) accession number: P02992
Secondary accession number(s): D6W2P3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: May 23, 2018
This is version 166 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

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