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P02992

- EFTU_YEAST

UniProt

P02992 - EFTU_YEAST

Protein

Elongation factor Tu, mitochondrial

Gene

TUF1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 136 (01 Oct 2014)
      Sequence version 1 (21 Jul 1986)
      Previous versions | rss
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    Functioni

    G-protein that, in its active GTP-bound form, binds to and delivers aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. In the presence of a correct codon-anticodon match between the aminoacyl-tRNA and the A-site codon of the ribosome-bound mRNA, the ribosome acts as a GTPase activator and the GTP is hydrolyzed. The inactive GDP-bound form leaves the ribosome and must be recycled before binding another molecule of aminoacyl-tRNA. Required for mitochondrial protein biosynthesis and maintenance of mitochondrial DNA.1 Publication

    Pathwayi

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi55 – 628GTPBy similarity
    Nucleotide bindingi117 – 1215GTPBy similarity
    Nucleotide bindingi172 – 1754GTPBy similarity

    GO - Molecular functioni

    1. GTPase activity Source: SGD
    2. GTP binding Source: UniProtKB-KW
    3. translation elongation factor activity Source: SGD

    GO - Biological processi

    1. GTP catabolic process Source: GOC
    2. mitochondrial translation Source: SGD
    3. mitochondrial translational elongation Source: SGD

    Keywords - Molecular functioni

    Elongation factor

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33697-MONOMER.
    UniPathwayiUPA00345.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Elongation factor Tu, mitochondrial
    Alternative name(s):
    tufM
    Gene namesi
    Name:TUF1
    Synonyms:TUFM
    Ordered Locus Names:YOR187W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XV

    Organism-specific databases

    CYGDiYOR187w.
    SGDiS000005713. TUF1.

    Subcellular locationi

    Mitochondrion 2 Publications

    GO - Cellular componenti

    1. mitochondrial matrix Source: SGD

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 3838MitochondrionCuratedAdd
    BLAST
    Chaini39 – 437399Elongation factor Tu, mitochondrialPRO_0000007465Add
    BLAST

    Post-translational modificationi

    The precursor is processed in two steps involving mitochondrial intermediate peptidase (MIP) and mitochondrial processing peptidase (MPP).1 Publication

    Proteomic databases

    MaxQBiP02992.
    PaxDbiP02992.
    PeptideAtlasiP02992.
    PRIDEiP02992.

    Expressioni

    Gene expression databases

    GenevestigatoriP02992.

    Interactioni

    Protein-protein interaction databases

    BioGridi34582. 68 interactions.
    DIPiDIP-4130N.
    IntActiP02992. 12 interactions.
    MINTiMINT-497322.
    STRINGi4932.YOR187W.

    Structurei

    3D structure databases

    ProteinModelPortaliP02992.
    SMRiP02992. Positions 45-437.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini46 – 242197tr-type GAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni55 – 628G1By similarity
    Regioni96 – 1005G2By similarity
    Regioni117 – 1204G3By similarity
    Regioni172 – 1754G4By similarity
    Regioni210 – 2123G5By similarity

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0050.
    GeneTreeiENSGT00550000074682.
    HOGENOMiHOG000229290.
    KOiK02358.
    OMAiGTEMCMP.
    OrthoDBiEOG7GXPMZ.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    HAMAPiMF_00118_B. EF_Tu_B.
    InterProiIPR000795. EF_GTP-bd_dom.
    IPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR009000. Transl_B-barrel.
    IPR009001. Transl_elong_EF1A/Init_IF2_C.
    IPR004161. Transl_elong_EFTu/EF1A_2.
    IPR004541. Transl_elong_EFTu/EF1A_bac/org.
    IPR004160. Transl_elong_EFTu/EF1A_C.
    [Graphical view]
    PfamiPF00009. GTP_EFTU. 1 hit.
    PF03144. GTP_EFTU_D2. 1 hit.
    PF03143. GTP_EFTU_D3. 1 hit.
    [Graphical view]
    PRINTSiPR00315. ELONGATNFCT.
    SUPFAMiSSF50447. SSF50447. 1 hit.
    SSF50465. SSF50465. 1 hit.
    SSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR00485. EF-Tu. 1 hit.
    TIGR00231. small_GTP. 1 hit.
    PROSITEiPS00301. G_TR_1. 1 hit.
    PS51722. G_TR_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P02992-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSALLPRLLT RTAFKASGKL LRLSSVISRT FSQTTTSYAA AFDRSKPHVN    50
    IGTIGHVDHG KTTLTAAITK TLAAKGGANF LDYAAIDKAP EERARGITIS 100
    TAHVEYETAK RHYSHVDCPG HADYIKNMIT GAAQMDGAII VVAATDGQMP 150
    QTREHLLLAR QVGVQHIVVF VNKVDTIDDP EMLELVEMEM RELLNEYGFD 200
    GDNAPIIMGS ALCALEGRQP EIGEQAIMKL LDAVDEYIPT PERDLNKPFL 250
    MPVEDIFSIS GRGTVVTGRV ERGNLKKGEE LEIVGHNSTP LKTTVTGIEM 300
    FRKELDSAMA GDNAGVLLRG IRRDQLKRGM VLAKPGTVKA HTKILASLYI 350
    LSKEEGGRHS GFGENYRPQM FIRTADVTVV MRFPKEVEDH SMQVMPGDNV 400
    EMECDLIHPT PLEVGQRFNI REGGRTVGTG LITRIIE 437
    Length:437
    Mass (Da):47,972
    Last modified:July 21, 1986 - v1
    Checksum:i26F4DFE805D1A93B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    K00428 Genomic DNA. No translation available.
    Z75095 Genomic DNA. Translation: CAA99396.1.
    BK006948 Genomic DNA. Translation: DAA10959.1.
    PIRiA03520. EFBYT.
    RefSeqiNP_014830.1. NM_001183606.1.

    Genome annotation databases

    EnsemblFungiiYOR187W; YOR187W; YOR187W.
    GeneIDi854359.
    KEGGisce:YOR187W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    K00428 Genomic DNA. No translation available.
    Z75095 Genomic DNA. Translation: CAA99396.1 .
    BK006948 Genomic DNA. Translation: DAA10959.1 .
    PIRi A03520. EFBYT.
    RefSeqi NP_014830.1. NM_001183606.1.

    3D structure databases

    ProteinModelPortali P02992.
    SMRi P02992. Positions 45-437.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34582. 68 interactions.
    DIPi DIP-4130N.
    IntActi P02992. 12 interactions.
    MINTi MINT-497322.
    STRINGi 4932.YOR187W.

    Proteomic databases

    MaxQBi P02992.
    PaxDbi P02992.
    PeptideAtlasi P02992.
    PRIDEi P02992.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YOR187W ; YOR187W ; YOR187W .
    GeneIDi 854359.
    KEGGi sce:YOR187W.

    Organism-specific databases

    CYGDi YOR187w.
    SGDi S000005713. TUF1.

    Phylogenomic databases

    eggNOGi COG0050.
    GeneTreei ENSGT00550000074682.
    HOGENOMi HOG000229290.
    KOi K02358.
    OMAi GTEMCMP.
    OrthoDBi EOG7GXPMZ.

    Enzyme and pathway databases

    UniPathwayi UPA00345 .
    BioCyci YEAST:G3O-33697-MONOMER.

    Miscellaneous databases

    NextBioi 976464.
    PROi P02992.

    Gene expression databases

    Genevestigatori P02992.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    HAMAPi MF_00118_B. EF_Tu_B.
    InterProi IPR000795. EF_GTP-bd_dom.
    IPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR009000. Transl_B-barrel.
    IPR009001. Transl_elong_EF1A/Init_IF2_C.
    IPR004161. Transl_elong_EFTu/EF1A_2.
    IPR004541. Transl_elong_EFTu/EF1A_bac/org.
    IPR004160. Transl_elong_EFTu/EF1A_C.
    [Graphical view ]
    Pfami PF00009. GTP_EFTU. 1 hit.
    PF03144. GTP_EFTU_D2. 1 hit.
    PF03143. GTP_EFTU_D3. 1 hit.
    [Graphical view ]
    PRINTSi PR00315. ELONGATNFCT.
    SUPFAMi SSF50447. SSF50447. 1 hit.
    SSF50465. SSF50465. 1 hit.
    SSF52540. SSF52540. 1 hit.
    TIGRFAMsi TIGR00485. EF-Tu. 1 hit.
    TIGR00231. small_GTP. 1 hit.
    PROSITEi PS00301. G_TR_1. 1 hit.
    PS51722. G_TR_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and sequence determination of the nuclear gene coding for mitochondrial elongation factor Tu of Saccharomyces cerevisiae."
      Nagata S., Tsunetsugu-Yokota Y., Naito A., Kaziro Y.
      Proc. Natl. Acad. Sci. U.S.A. 80:6192-6196(1983) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
      Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
      , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
      Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Mitochondrial protein synthesis is required for maintenance of intact mitochondrial genomes in Saccharomyces cerevisiae."
      Myers A.M., Pape L.K., Tzagoloff A.
      EMBO J. 4:2087-2092(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    5. "Prediction and identification of new natural substrates of the yeast mitochondrial intermediate peptidase."
      Branda S.S., Isaya G.
      J. Biol. Chem. 270:27366-27373(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: CLEAVAGE BY MPP AND MIP, IDENTIFICATION OF PROBABLE CLEAVAGE SITE.
    6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    8. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
      Strain: ATCC 76625 / YPH499.
    9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiEFTU_YEAST
    AccessioniPrimary (citable) accession number: P02992
    Secondary accession number(s): D6W2P3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: July 21, 1986
    Last modified: October 1, 2014
    This is version 136 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 58527 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XV
      Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

    External Data

    Dasty 3