Reviewed, UniProtKB/Swiss-Prot P02979 (ERMC1_STAAU)

Names and origin

Protein names

Recommended name:
    rRNA adenine N-6-methyltransferase
    EC=2.1.1.48
Alternative name(s):
    Macrolide-lincosamide-streptogramin B resistance protein
    Erythromycin resistance protein

Gene names

Name:

ermC

Encoded on

Plasmid pE194 Ref.1 Ref.2 Ref.5
Plasmid pT48 Ref.3
Plasmid pA22 Ref.4

Organism

Staphylococcus aureus

Taxonomic identifier

1280 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	244 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	This protein produces a dimethylation of the adenine residue at position 2058 in 23S rRNA, resulting in reduced affinity between ribosomes and macrolide-lincosamide-streptogramin B antibiotics.
Catalytic activity	S-adenosyl-L-methionine + rRNA = S-adenosyl-L-homocysteine + rRNA containing N(6)-methyladenine.
Induction	By erythromycin.
Sequence similarities	Belongs to the rRNA adenine N(6)-methyltransferase family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Ligand	RNA-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Plasmid
Gene Ontology (GO)
Biological process	rRNA modification Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW rRNA (adenine-N6,N6-)-dimethyltransferase activity Inferred from electronic annotation. Source: InterPro rRNA (adenine-N6-)-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 244	244	rRNA adenine N-6-methyltransferase		PRO_0000101682
Sites
	Binding site	11	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	13	1	S-adenosyl-L-methionine; via amide nitrogen By similarity
	Binding site	38	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	59	1	S-adenosyl-L-methionine By similarity
	Binding site	84	1	S-adenosyl-L-methionine By similarity
	Binding site	101	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P02979-1 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: 3CA9A267C4FE3C30	FASTA	244	28,861
	10 20 30 40 50 60 MNEKNIKHSQ NFITSKHNID KIMTNIRLNE HDNIFEIGSG KGHFTLELVK RCNFVTAIEI 70 80 90 100 110 120 DHKLCKTTEN KLVDHDNFQV LNKDILQFKF PKNQSYKIYG NIPYNISTDI IRKIVFDSIA 130 140 150 160 170 180 NEIYLIVEYG FAKRLLNTKR SLALLLMAEV DISILSMVPR EYFHPKPKVN SSLIRLSRKK 190 200 210 220 230 240 SRISHKDKQK YNYFVMKWVN KEYKKIFTKN QFNNSLKHAG IDDLNNISFE QFLSLFNSYK LFNK

References

[1]	"Conformational alteration of mRNA structure and the posttranscriptional regulation of erythromycin-induced drug resistance." Gryczan T.J., Grandi G., Hahn J., Grandi R., Dubnau D. Nucleic Acids Res. 8:6081-6097(1980) [PubMed: 6162157] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Posttranscriptional modification of mRNA conformation: mechanism that regulates erythromycin-induced resistance." Horinouchi S., Weisblum B. Proc. Natl. Acad. Sci. U.S.A. 77:7079-7083(1980) [PubMed: 6938954] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The nucleotide sequence of Staphylococcus aureus plasmid pT48 conferring inducible macrolide-lincosamide-streptogramin B resistance and comparison with similar plasmids expressing constitutive resistance." Catchpole I., Thomas C., Davies A., Dyke K.G.H. J. Gen. Microbiol. 134:697-709(1988) [PubMed: 3141573] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-48.
[4]	"A Staphylococcus aureus plasmid that specifies constitutive macrolide-lincosamide-streptogramin B resistance contains a novel deletion in the ermC attenuator." Catchpole I., Dyke K.G.H. FEMS Microbiol. Lett. 57:43-47(1990) [PubMed: 2116351] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-40.
[5]	"Messenger RNA from Staphylococcus aureus that specifies macrolide-lincosamide-streptogramin resistance. Demonstration of its conformations and of the leader peptide it encodes." Mayford M., Weisblum B. J. Mol. Biol. 185:769-780(1985) [PubMed: 2414456] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-11.

Cross-references

Sequence databases
EMBLGenBankDDBJ	V01278 Genomic DNA. Translation: CAA24591.1. M37841 Genomic DNA. Translation: AAA98226.1. X54338 Genomic DNA. Translation: CAA38227.1.
PIR	YESA9E. A93717.
RefSeq	YP_025321.1.
3D structure databases
HSSP	HSSP built from PDB template 1QAN based on UniProtKB P13956.
SMR	P02979. Positions 9-244.
ModBase	Search...
Genome annotation databases
GeneID	3284064.
Enzyme and pathway databases
BRENDA	2.1.1.48. 95.
Family and domain databases
InterPro	IPR001737. RRNA_Ade_Mease_Trfase. [Graphical view]
PANTHER	PTHR11727. RRNA_meth_trans. 1 hit.
Pfam	PF00398. RrnaAD. 1 hit. [Graphical view]
SMART	SM00650. rADc. 1 hit. [Graphical view]
PROSITE	PS01131. RRNA_A_DIMETH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ERMC1_STAAU

Accession

Primary (citable) accession number: P02979

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	May 5, 2009
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)