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Protein

Colicin-E1

Gene

cea

Organism
Escherichia coli
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

This colicin is a channel-forming colicin. This class of transmembrane toxins depolarize the cytoplasmic membrane, leading to dissipation of cellular energy.
Colicins are polypeptide toxins produced by and active against E.coli and closely related bacteria.

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Antibiotic, Antimicrobial, Bacteriocin

Protein family/group databases

TCDBi1.C.1.2.2. the channel-forming colicin (colicin) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Colicin-E1
Gene namesi
Name:cea
Encoded oniPlasmid ColE12 Publications
Plasmid ColE1-EC711 Publication
Plasmid pAO31 Publication
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei471 – 487HelicalSequence analysisAdd BLAST17
Transmembranei494 – 510HelicalSequence analysisAdd BLAST17

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002186681 – 522Colicin-E1Add BLAST522

Proteomic databases

PRIDEiP02978.

Structurei

Secondary structure

1522
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi346 – 362Combined sources17
Helixi365 – 378Combined sources14
Helixi386 – 402Combined sources17
Helixi406 – 416Combined sources11
Helixi422 – 425Combined sources4
Helixi428 – 434Combined sources7
Helixi447 – 457Combined sources11
Helixi461 – 471Combined sources11
Helixi472 – 475Combined sources4
Helixi476 – 488Combined sources13
Helixi494 – 507Combined sources14
Helixi513 – 515Combined sources3
Helixi516 – 520Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2I88X-ray2.50A332-522[»]
ProteinModelPortaliP02978.
SMRiP02978.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP02978.

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi31 – 42Poly-GlyAdd BLAST12

Sequence similaritiesi

Belongs to the channel forming colicin family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Family and domain databases

Gene3Di1.10.490.30. 1 hit.
InterProiIPR000293. Channel_colicin_C.
[Graphical view]
PfamiPF01024. Colicin. 1 hit.
[Graphical view]
PRINTSiPR00280. CHANLCOLICIN.
ProDomiPD002657. Channel_colicin_C. 1 hit.
[Graphical view] [Entries sharing at least one domain]
PROSITEiPS00276. CHANNEL_COLICIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P02978-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
METAVAYYKD GVPYDDKGQV IITLLNGTPD GSGSGGGGGK GGSKSESSAA
60 70 80 90 100
IHATAKWSTA QLKKTQAEQA ARAKAAAEAQ AKAKANRDAL TQRLKDIVNE
110 120 130 140 150
ALRHNASRTP SATELAHANN AAMQAEDERL RLAKAEEKAR KEAEAAEKAF
160 170 180 190 200
QEAEQRRKEI EREKAETERQ LKLAEAEEKR LAALSEEAKA VEIAQKKLSA
210 220 230 240 250
AQSEVVKMDG EIKTLNSRLS SSIHARDAEM KTLAGKRNEL AQASAKYKEL
260 270 280 290 300
DELVKKLSPR ANDPLQNRPF FEATRRRVGA GKIREEKQKQ VTASETRINR
310 320 330 340 350
INADITQIQK AISQVSNNRN AGIARVHEAE ENLKKAQNNL LNSQIKDAVD
360 370 380 390 400
ATVSFYQTLT EKYGEKYSKM AQELADKSKG KKIGNVNEAL AAFEKYKDVL
410 420 430 440 450
NKKFSKADRD AIFNALASVK YDDWAKHLDQ FAKYLKITGH VSFGYDVVSD
460 470 480 490 500
ILKIKDTGDW KPLFLTLEKK AADAGVSYVV ALLFSLLAGT TLGIWGIAIV
510 520
TGILCSYIDK NKLNTINEVL GI
Length:522
Mass (Da):57,280
Last modified:July 21, 1986 - v1
Checksum:iA77C351BBC1AB7C1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01563 Genomic DNA. Translation: AAA87379.1.
U15633 Genomic DNA. Translation: AAA59418.1.
PIRiA93913. IKEC1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01563 Genomic DNA. Translation: AAA87379.1.
U15633 Genomic DNA. Translation: AAA59418.1.
PIRiA93913. IKEC1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2I88X-ray2.50A332-522[»]
ProteinModelPortaliP02978.
SMRiP02978.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

TCDBi1.C.1.2.2. the channel-forming colicin (colicin) family.

Proteomic databases

PRIDEiP02978.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP02978.

Family and domain databases

Gene3Di1.10.490.30. 1 hit.
InterProiIPR000293. Channel_colicin_C.
[Graphical view]
PfamiPF01024. Colicin. 1 hit.
[Graphical view]
PRINTSiPR00280. CHANLCOLICIN.
ProDomiPD002657. Channel_colicin_C. 1 hit.
[Graphical view] [Entries sharing at least one domain]
PROSITEiPS00276. CHANNEL_COLICIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCEA1_ECOLX
AccessioniPrimary (citable) accession number: P02978
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: November 2, 2016
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Plasmid PAO3 is a small colicin E1 derivative.

Keywords - Technical termi

3D-structure, Plasmid

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.