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Protein

Immunoglobulin G-binding protein A

Gene

spa

Organism
Staphylococcus aureus (strain NCTC 8325)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

GO - Biological processi

  • pathogenesis Source: CACAO
Complete GO annotation...

Keywords - Ligandi

IgG-binding protein

Enzyme and pathway databases

BioCyciSAUR93061:GIWJ-64-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Immunoglobulin G-binding protein A
Short name:
IgG-binding protein A
Alternative name(s):
Staphylococcal protein A
Gene namesi
Name:spa
Ordered Locus Names:SAOUHSC_00069
OrganismiStaphylococcus aureus (strain NCTC 8325)
Taxonomic identifieri93061 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000008816 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell wall, Secreted

Pathology & Biotechi

Disruption phenotypei

No visible phenotype.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3636Sequence analysisAdd
BLAST
Chaini37 – 485449Immunoglobulin G-binding protein APRO_0000005653Add
BLAST
Propeptidei486 – 51631Removed by sortasePROSITE-ProRule annotationPRO_0000005654Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei485 – 4851Pentaglycyl murein peptidoglycan amidated threoninePROSITE-ProRule annotation

Keywords - PTMi

Peptidoglycan-anchor

Expressioni

Inductioni

More protein is secreted in a secG or double secG/secY2 mutant (at protein level).1 Publication

Interactioni

Protein-protein interaction databases

MINTiMINT-1206155.
STRINGi93061.SAOUHSC_00069.

Structurei

Secondary structure

1
516
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi104 – 11310Combined sources
Beta strandi115 – 1173Combined sources
Helixi119 – 13113Combined sources
Helixi133 – 1353Combined sources
Helixi136 – 14914Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1DEEX-ray2.70G/H100-153[»]
4Y4YX-ray3.00A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T/U/V/W/X/Y/Z/a/b/c/d158-211[»]
A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T/U/V/W/X/Y/Z/a/b/c/d219-354[»]
A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T/U/V/W/X/Y/Z/a/b/c/d236-459[»]
ProteinModelPortaliP02976.
SMRiP02976. Positions 37-327.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP02976.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati37 – 8852B 1Add
BLAST
Repeati96 – 14954B 2Add
BLAST
Repeati154 – 20754B 3Add
BLAST
Repeati212 – 26554B 4Add
BLAST
Repeati266 – 32358B 5Add
BLAST
Repeati333 – 34082-1
Repeati341 – 34882-2
Repeati349 – 35682-3
Repeati357 – 36482-4
Repeati365 – 37282-5
Repeati373 – 38082-6
Repeati381 – 38882-7
Repeati389 – 39682-8
Repeati397 – 40482-9
Repeati405 – 41282-10
Repeati413 – 42082-11
Domaini421 – 46545LysMPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni333 – 4208811 X 8 AA approximate tandem repeatsAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi482 – 4865LPXTG sorting signalPROSITE-ProRule annotation

Sequence similaritiesi

Contains 1 LysM domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiENOG4106I9N. Bacteria.
ENOG410XXBQ. LUCA.
HOGENOMiHOG000279707.
KOiK14196.
OMAiDAQQNKF.
OrthoDBiEOG69PPZZ.

Family and domain databases

Gene3Di3.10.350.10. 1 hit.
InterProiIPR019948. Gram-positive_anchor.
IPR009063. Ig/albumin-bd.
IPR019931. LPXTG_anchor.
IPR018392. LysM_dom.
IPR005038. Octapeptide.
IPR003132. Protein_A_Ig-bd.
IPR005877. YSIRK_signal_dom.
[Graphical view]
PfamiPF02216. B. 5 hits.
PF00746. Gram_pos_anchor. 1 hit.
PF01476. LysM. 1 hit.
PF03373. Octapeptide. 12 hits.
PF04650. YSIRK_signal. 1 hit.
[Graphical view]
SMARTiSM00257. LysM. 1 hit.
[Graphical view]
SUPFAMiSSF46997. SSF46997. 5 hits.
SSF54106. SSF54106. 1 hit.
TIGRFAMsiTIGR01167. LPXTG_anchor. 1 hit.
TIGR01168. YSIRK_signal. 1 hit.
PROSITEiPS50847. GRAM_POS_ANCHORING. 1 hit.
PS51782. LYSM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P02976-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKKNIYSIR KLGVGIASVT LGTLLISGGV TPAANAAQHD EAQQNAFYQV
60 70 80 90 100
LNMPNLNADQ RNGFIQSLKD DPSQSANVLG EAQKLNDSQA PKADAQQNNF
110 120 130 140 150
NKDQQSAFYE ILNMPNLNEA QRNGFIQSLK DDPSQSTNVL GEAKKLNESQ
160 170 180 190 200
APKADNNFNK EQQNAFYEIL NMPNLNEEQR NGFIQSLKDD PSQSANLLSE
210 220 230 240 250
AKKLNESQAP KADNKFNKEQ QNAFYEILHL PNLNEEQRNG FIQSLKDDPS
260 270 280 290 300
QSANLLAEAK KLNDAQAPKA DNKFNKEQQN AFYEILHLPN LTEEQRNGFI
310 320 330 340 350
QSLKDDPSVS KEILAEAKKL NDAQAPKEED NNKPGKEDNN KPGKEDNNKP
360 370 380 390 400
GKEDNNKPGK EDNNKPGKED GNKPGKEDNK KPGKEDGNKP GKEDNKKPGK
410 420 430 440 450
EDGNKPGKED GNKPGKEDGN GVHVVKPGDT VNDIAKANGT TADKIAADNK
460 470 480 490 500
LADKNMIKPG QELVVDKKQP ANHADANKAQ ALPETGEENP FIGTTVFGGL
510
SLALGAALLA GRRREL
Length:516
Mass (Da):56,437
Last modified:July 11, 2006 - v3
Checksum:iC6FEFFF9E54BE1F0
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti101 – 1011N → D in CAA24596 (PubMed:6338496).Curated
Sequence conflicti370 – 3701D → DNNKPGKED in AAA26676 (PubMed:6319407).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01786 Genomic DNA. Translation: AAA26676.1.
CP000253 Genomic DNA. Translation: ABD29253.1.
V01287 Genomic DNA. Translation: CAA24596.1.
PIRiA58531. QVSAA.
RefSeqiWP_000728764.1. NC_007795.1.
YP_498670.1. NC_007795.1.

Genome annotation databases

EnsemblBacteriaiABD29253; ABD29253; SAOUHSC_00069.
GeneIDi3919448.
KEGGisao:SAOUHSC_00069.
PATRICi19577766. VBIStaAur99865_0059.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01786 Genomic DNA. Translation: AAA26676.1.
CP000253 Genomic DNA. Translation: ABD29253.1.
V01287 Genomic DNA. Translation: CAA24596.1.
PIRiA58531. QVSAA.
RefSeqiWP_000728764.1. NC_007795.1.
YP_498670.1. NC_007795.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1DEEX-ray2.70G/H100-153[»]
4Y4YX-ray3.00A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T/U/V/W/X/Y/Z/a/b/c/d158-211[»]
A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T/U/V/W/X/Y/Z/a/b/c/d219-354[»]
A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T/U/V/W/X/Y/Z/a/b/c/d236-459[»]
ProteinModelPortaliP02976.
SMRiP02976. Positions 37-327.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-1206155.
STRINGi93061.SAOUHSC_00069.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABD29253; ABD29253; SAOUHSC_00069.
GeneIDi3919448.
KEGGisao:SAOUHSC_00069.
PATRICi19577766. VBIStaAur99865_0059.

Phylogenomic databases

eggNOGiENOG4106I9N. Bacteria.
ENOG410XXBQ. LUCA.
HOGENOMiHOG000279707.
KOiK14196.
OMAiDAQQNKF.
OrthoDBiEOG69PPZZ.

Enzyme and pathway databases

BioCyciSAUR93061:GIWJ-64-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP02976.
PROiP02976.

Family and domain databases

Gene3Di3.10.350.10. 1 hit.
InterProiIPR019948. Gram-positive_anchor.
IPR009063. Ig/albumin-bd.
IPR019931. LPXTG_anchor.
IPR018392. LysM_dom.
IPR005038. Octapeptide.
IPR003132. Protein_A_Ig-bd.
IPR005877. YSIRK_signal_dom.
[Graphical view]
PfamiPF02216. B. 5 hits.
PF00746. Gram_pos_anchor. 1 hit.
PF01476. LysM. 1 hit.
PF03373. Octapeptide. 12 hits.
PF04650. YSIRK_signal. 1 hit.
[Graphical view]
SMARTiSM00257. LysM. 1 hit.
[Graphical view]
SUPFAMiSSF46997. SSF46997. 5 hits.
SSF54106. SSF54106. 1 hit.
TIGRFAMsiTIGR01167. LPXTG_anchor. 1 hit.
TIGR01168. YSIRK_signal. 1 hit.
PROSITEiPS50847. GRAM_POS_ANCHORING. 1 hit.
PS51782. LYSM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete sequence of the staphylococcal gene encoding protein A. A gene evolved through multiple duplications."
    Uhlen M., Guss B., Nilsson B., Gatenbeck S., Philipson L., Lindberg M.
    J. Biol. Chem. 259:1695-1702(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Erratum
    Uhlen M., Guss B., Nilsson B., Gatenbeck S., Philipson L., Lindberg M.
    J. Biol. Chem. 259:13628-13628(1984)
    Cited for: SEQUENCE REVISION.
  3. "The Staphylococcus aureus NCTC 8325 genome."
    Gillaspy A.F., Worrell V., Orvis J., Roe B.A., Dyer D.W., Iandolo J.J.
    (In) Fischetti V., Novick R., Ferretti J., Portnoy D., Rood J. (eds.); Gram positive pathogens, 2nd edition, pp.381-412, ASM Press, Washington D.C. (2006)
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NCTC 8325.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-186.
  5. "Synthetic effects of secG and secY2 mutations on exoproteome biogenesis in Staphylococcus aureus."
    Sibbald M.J., Winter T., van der Kooi-Pol M.M., Buist G., Tsompanidou E., Bosma T., Schafer T., Ohlsen K., Hecker M., Antelmann H., Engelmann S., van Dijl J.M.
    J. Bacteriol. 192:3788-3800(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION, INDUCTION.
    Strain: RN4220.
  6. "New range of vectors with a stringent 5-fluoroorotic acid-based counterselection system for generating mutants by allelic replacement in Staphylococcus aureus."
    Redder P., Linder P.
    Appl. Environ. Microbiol. 78:3846-3854(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.
    Strain: SA564.

Entry informationi

Entry nameiSPA_STAA8
AccessioniPrimary (citable) accession number: P02976
Secondary accession number(s): Q2G1N8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 11, 2006
Last modified: June 8, 2016
This is version 146 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Important immunodiagnostic reagent because of its ability to bind the Fc fragment of a wide range of mammalian immunoglobulins.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.