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Protein

Immunoglobulin G-binding protein A

Gene

spa

Organism
Staphylococcus aureus (strain NCTC 8325)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

GO - Biological processi

  • pathogenesis Source: CACAO
Complete GO annotation...

Keywords - Ligandi

IgG-binding protein

Names & Taxonomyi

Protein namesi
Recommended name:
Immunoglobulin G-binding protein A
Short name:
IgG-binding protein A
Alternative name(s):
Staphylococcal protein A
Gene namesi
Name:spa
Ordered Locus Names:SAOUHSC_00069
OrganismiStaphylococcus aureus (strain NCTC 8325)
Taxonomic identifieri93061 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000008816 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell wall, Secreted

Pathology & Biotechi

Disruption phenotypei

No visible phenotype.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 36Sequence analysisAdd BLAST36
ChainiPRO_000000565337 – 485Immunoglobulin G-binding protein AAdd BLAST449
PropeptideiPRO_0000005654486 – 516Removed by sortasePROSITE-ProRule annotationAdd BLAST31

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei485Pentaglycyl murein peptidoglycan amidated threoninePROSITE-ProRule annotation1

Keywords - PTMi

Peptidoglycan-anchor

Proteomic databases

PRIDEiP02976.

Expressioni

Inductioni

More protein is secreted in a secG or double secG/secY2 mutant (at protein level).1 Publication

Interactioni

Protein-protein interaction databases

MINTiMINT-1206155.
STRINGi93061.SAOUHSC_00069.

Structurei

Secondary structure

1516
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi104 – 113Combined sources10
Beta strandi115 – 117Combined sources3
Helixi119 – 131Combined sources13
Helixi133 – 135Combined sources3
Helixi136 – 149Combined sources14
Beta strandi242 – 255Combined sources14
Beta strandi260 – 265Combined sources6
Helixi268 – 271Combined sources4
Helixi273 – 279Combined sources7
Beta strandi282 – 289Combined sources8
Beta strandi295 – 301Combined sources7
Beta strandi310 – 316Combined sources7
Helixi327 – 330Combined sources4
Beta strandi333 – 335Combined sources3
Beta strandi348 – 352Combined sources5
Helixi357 – 363Combined sources7
Beta strandi381 – 384Combined sources4
Helixi387 – 389Combined sources3
Helixi407 – 415Combined sources9
Turni423 – 425Combined sources3
Beta strandi429 – 435Combined sources7
Beta strandi443 – 459Combined sources17

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DEEX-ray2.70G/H100-153[»]
4Y4YX-ray3.00A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T/U/V/W/X/Y/Z/a/b/c/d158-211[»]
A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T/U/V/W/X/Y/Z/a/b/c/d219-354[»]
A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T/U/V/W/X/Y/Z/a/b/c/d236-459[»]
ProteinModelPortaliP02976.
SMRiP02976.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP02976.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati37 – 88B 1Add BLAST52
Repeati96 – 149B 2Add BLAST54
Repeati154 – 207B 3Add BLAST54
Repeati212 – 265B 4Add BLAST54
Repeati266 – 323B 5Add BLAST58
Repeati333 – 3402-18
Repeati341 – 3482-28
Repeati349 – 3562-38
Repeati357 – 3642-48
Repeati365 – 3722-58
Repeati373 – 3802-68
Repeati381 – 3882-78
Repeati389 – 3962-88
Repeati397 – 4042-98
Repeati405 – 4122-108
Repeati413 – 4202-118
Domaini421 – 465LysMPROSITE-ProRule annotationAdd BLAST45

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni333 – 42011 X 8 AA approximate tandem repeatsAdd BLAST88

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi482 – 486LPXTG sorting signalPROSITE-ProRule annotation5

Sequence similaritiesi

Contains 1 LysM domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiENOG4106I9N. Bacteria.
ENOG410XXBQ. LUCA.
HOGENOMiHOG000279707.
KOiK14196.
OMAiDAQQNKF.

Family and domain databases

CDDicd00118. LysM. 1 hit.
Gene3Di3.10.350.10. 1 hit.
InterProiIPR019948. Gram-positive_anchor.
IPR009063. Ig/albumin-bd.
IPR018392. LysM_dom.
IPR005038. Octapeptide.
IPR003132. Protein_A_Ig-bd.
IPR005877. YSIRK_signal_dom.
[Graphical view]
PfamiPF02216. B. 5 hits.
PF00746. Gram_pos_anchor. 1 hit.
PF01476. LysM. 1 hit.
PF03373. Octapeptide. 12 hits.
PF04650. YSIRK_signal. 1 hit.
[Graphical view]
SMARTiSM00257. LysM. 1 hit.
[Graphical view]
SUPFAMiSSF46997. SSF46997. 5 hits.
SSF54106. SSF54106. 1 hit.
TIGRFAMsiTIGR01168. YSIRK_signal. 1 hit.
PROSITEiPS50847. GRAM_POS_ANCHORING. 1 hit.
PS51782. LYSM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P02976-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKKNIYSIR KLGVGIASVT LGTLLISGGV TPAANAAQHD EAQQNAFYQV
60 70 80 90 100
LNMPNLNADQ RNGFIQSLKD DPSQSANVLG EAQKLNDSQA PKADAQQNNF
110 120 130 140 150
NKDQQSAFYE ILNMPNLNEA QRNGFIQSLK DDPSQSTNVL GEAKKLNESQ
160 170 180 190 200
APKADNNFNK EQQNAFYEIL NMPNLNEEQR NGFIQSLKDD PSQSANLLSE
210 220 230 240 250
AKKLNESQAP KADNKFNKEQ QNAFYEILHL PNLNEEQRNG FIQSLKDDPS
260 270 280 290 300
QSANLLAEAK KLNDAQAPKA DNKFNKEQQN AFYEILHLPN LTEEQRNGFI
310 320 330 340 350
QSLKDDPSVS KEILAEAKKL NDAQAPKEED NNKPGKEDNN KPGKEDNNKP
360 370 380 390 400
GKEDNNKPGK EDNNKPGKED GNKPGKEDNK KPGKEDGNKP GKEDNKKPGK
410 420 430 440 450
EDGNKPGKED GNKPGKEDGN GVHVVKPGDT VNDIAKANGT TADKIAADNK
460 470 480 490 500
LADKNMIKPG QELVVDKKQP ANHADANKAQ ALPETGEENP FIGTTVFGGL
510
SLALGAALLA GRRREL
Length:516
Mass (Da):56,437
Last modified:July 11, 2006 - v3
Checksum:iC6FEFFF9E54BE1F0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti101N → D in CAA24596 (PubMed:6338496).Curated1
Sequence conflicti370D → DNNKPGKED in AAA26676 (PubMed:6319407).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01786 Genomic DNA. Translation: AAA26676.1.
CP000253 Genomic DNA. Translation: ABD29253.1.
V01287 Genomic DNA. Translation: CAA24596.1.
PIRiA58531. QVSAA.
RefSeqiWP_000728764.1. NC_007795.1.
YP_498670.1. NC_007795.1.

Genome annotation databases

EnsemblBacteriaiABD29253; ABD29253; SAOUHSC_00069.
GeneIDi3919448.
KEGGisao:SAOUHSC_00069.
PATRICi19577766. VBIStaAur99865_0059.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01786 Genomic DNA. Translation: AAA26676.1.
CP000253 Genomic DNA. Translation: ABD29253.1.
V01287 Genomic DNA. Translation: CAA24596.1.
PIRiA58531. QVSAA.
RefSeqiWP_000728764.1. NC_007795.1.
YP_498670.1. NC_007795.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DEEX-ray2.70G/H100-153[»]
4Y4YX-ray3.00A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T/U/V/W/X/Y/Z/a/b/c/d158-211[»]
A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T/U/V/W/X/Y/Z/a/b/c/d219-354[»]
A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T/U/V/W/X/Y/Z/a/b/c/d236-459[»]
ProteinModelPortaliP02976.
SMRiP02976.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-1206155.
STRINGi93061.SAOUHSC_00069.

Proteomic databases

PRIDEiP02976.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABD29253; ABD29253; SAOUHSC_00069.
GeneIDi3919448.
KEGGisao:SAOUHSC_00069.
PATRICi19577766. VBIStaAur99865_0059.

Phylogenomic databases

eggNOGiENOG4106I9N. Bacteria.
ENOG410XXBQ. LUCA.
HOGENOMiHOG000279707.
KOiK14196.
OMAiDAQQNKF.

Miscellaneous databases

EvolutionaryTraceiP02976.
PROiP02976.

Family and domain databases

CDDicd00118. LysM. 1 hit.
Gene3Di3.10.350.10. 1 hit.
InterProiIPR019948. Gram-positive_anchor.
IPR009063. Ig/albumin-bd.
IPR018392. LysM_dom.
IPR005038. Octapeptide.
IPR003132. Protein_A_Ig-bd.
IPR005877. YSIRK_signal_dom.
[Graphical view]
PfamiPF02216. B. 5 hits.
PF00746. Gram_pos_anchor. 1 hit.
PF01476. LysM. 1 hit.
PF03373. Octapeptide. 12 hits.
PF04650. YSIRK_signal. 1 hit.
[Graphical view]
SMARTiSM00257. LysM. 1 hit.
[Graphical view]
SUPFAMiSSF46997. SSF46997. 5 hits.
SSF54106. SSF54106. 1 hit.
TIGRFAMsiTIGR01168. YSIRK_signal. 1 hit.
PROSITEiPS50847. GRAM_POS_ANCHORING. 1 hit.
PS51782. LYSM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSPA_STAA8
AccessioniPrimary (citable) accession number: P02976
Secondary accession number(s): Q2G1N8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 11, 2006
Last modified: November 30, 2016
This is version 151 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Important immunodiagnostic reagent because of its ability to bind the Fc fragment of a wide range of mammalian immunoglobulins.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.