P02966 (DESS_MYXXA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Development-specific protein S

Alternative name(s):
Spore coat protein S

Gene names

Name:

tps

Organism

Myxococcus xanthus

Taxonomic identifier

34 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Myxococcales › Cystobacterineae › Myxococcaceae › Myxococcus

Protein attributes

Sequence length	173 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Protein S, induced in large amounts during fruiting body formation, assembles on the surface of myxospores in the presence of calcium ions.
Cofactor	Binds 2 calcium ions per subunit.
Domain	Has a two-domain beta-structure, folded into four very similar Greek key motifs.
Sequence similarities	Belongs to the beta/gamma-crystallin family. Contains 4 beta/gamma crystallin 'Greek key' domains.

Ontologies

Keywords
Biological process	Sporulation
Domain	Repeat
Ligand	Calcium
Technical term	3D-structure
Gene Ontology (GO)
Biological process	sporulation resulting in formation of a cellular spore Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 173

173

Development-specific protein S

PRO_0000057606

Regions

Domain

2 – 46

45

Beta/gamma crystallin 'Greek key' 1

Domain

48 – 86

39

Beta/gamma crystallin 'Greek key' 2

Domain

91 – 135

45

Beta/gamma crystallin 'Greek key' 3

Domain

136 – 173

38

Beta/gamma crystallin 'Greek key' 4

Calcium binding

32 – 46

15

Probable

Calcium binding

121 – 135

15

Probable

Region

87 – 90

4

Connecting peptide

Experimental info

Mutagenesis

40

1

S → R: Suppresses completely calcium-binding.

Mutagenesis

129

1

S → R: Lower calcium affinity.

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

173

Helix Strand Turn

Details...

Beta strand

4 – 9

6

Turn

10 – 12

3

Beta strand

13 – 19

7

Beta strand

21 – 24

4

Helix

26 – 31

6

Beta strand

41 – 43

3

Beta strand

48 – 55

8

Turn

56 – 58

3

Beta strand

59 – 67

9

Helix

73 – 75

3

Beta strand

81 – 86

6

Beta strand

93 – 95

3

Turn

99 – 101

3

Beta strand

106 – 108

3

Beta strand

110 – 113

4

Helix

119 – 122

4

Beta strand

134 – 144

11

Beta strand

148 – 153

6

Beta strand

161 – 165

5

Sequences

	Sequence		Length	Mass (Da)
	P02966 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: C504A6409752C454	FASTA	173	18,793
	10 20 30 40 50 60 MANITVFYNE DFQGKQVDLP PGNYTRAQLA ALGIENNTIS SVKVPPGVKA ILYQNDGFAG 70 80 90 100 110 120 DQIEVVANAE ELGPLNNNVS SIRVISVPVQ PRARFFYKEQ FDGKEVDLPP GQYTQAELER 130 140 150 160 170 YGIDNNTISS VKPQGLAVVL FKNDNFSGDT LPVNSDAPTL GAMNNNTSSI RIS

References

[1]	"Structural similarities between the development-specific protein S from a Gram-negative bacterium, Myxococcus xanthus, and calmodulin." Inouye S., Franceschini T., Inouye M. Proc. Natl. Acad. Sci. U.S.A. 80:6829-6833(1983) [PubMed: 6316328] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Characterization of calcium-binding sites in development-specific protein S of Myxococcus xanthus using site-specific mutagenesis." Teintze M., Inouye M., Inouye S. J. Biol. Chem. 263:1199-1203(1988) [PubMed: 3121626] [Abstract] Cited for: CALCIUM-BINDING SITES, MUTAGENESIS.
[3]	"Myxococcus xanthus spore coat protein S may have a similar structure to vertebrate lens beta gamma-crystallins." Wistow G., Summers L., Blundell T. Nature 315:771-773(1985) [PubMed: 3925350] [Abstract] Cited for: SIMILARITY TO BETA/GAMMA-CRYSTALLIN FAMILY.
[4]	"Structural similarity of a developmentally regulated bacterial spore coat protein to beta gamma-crystallins of the vertebrate eye lens." Bagby S., Harvey T.S., Eagle S.G., Inouye S., Ikura M. Proc. Natl. Acad. Sci. U.S.A. 91:4308-4312(1994) [PubMed: 8183906] [Abstract] Cited for: STRUCTURE BY NMR.
[5]	"NMR-derived three-dimensional solution structure of protein S complexed with calcium." Bagby S., Harvey T.S., Eagle S.G., Inouye S., Ikura M. Structure 2:107-122(1994) [PubMed: 8081742] [Abstract] Cited for: STRUCTURE BY NMR.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

J01745 Genomic DNA. Translation: AAA25407.1.

PIR

DZYZSX. A03490.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1NPS	X-ray	1.80	A	2-89	[»]
1PRR	NMR	-	A	1-173	[»]
1PRS	NMR	-	A	1-173	[»]

ProteinModelPortal

P02966.

SMR

P02966. Positions 1-173.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR001064. Beta/gamma_crystallin.
IPR011024. G_crystallin-rel.
[Graphical view]

Gene3D

G3DSA:2.60.20.10. Crystallin. 2 hits.

Pfam

PF00030. Crystall. 2 hits.
[Graphical view]

SMART

SM00247. XTALbg. 2 hits.
[Graphical view]

SUPFAM

SSF49695. G_crystallin_SF. 2 hits.

PROSITE

PS50915. CRYSTALLIN_BETA_GAMMA. 4 hits.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

DESS_MYXXA

Accession

Primary (citable) accession number: P02966

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	January 11, 2011
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program