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Protein

Chitooligosaccharide deacetylase

Gene

nodB

Organism
Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Is involved in generating a small heat-stable compound (Nod), an acylated oligomer of N-acetylglucosamine, that stimulates mitosis in various plant protoplasts.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei28Proton acceptorBy similarity1
Metal bindingi79Divalent metal cation; via tele nitrogenBy similarity1
Metal bindingi83Divalent metal cation; via tele nitrogenBy similarity1
Sitei148Raises pKa of active site HisBy similarity1
Active sitei174Proton donorBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Nodulation

Keywords - Ligandi

Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Chitooligosaccharide deacetylase (EC:3.5.1.-)
Alternative name(s):
Nodulation protein B
Gene namesi
Name:nodB
Ordered Locus Names:RA0474
ORF Names:SMa0868
Encoded oniPlasmid pSymA (megaplasmid 1)0 Publication
OrganismiRhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
Taxonomic identifieri266834 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium
Proteomesi
  • UP000001976 Componenti: Plasmid pSymA

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001727551 – 217Chitooligosaccharide deacetylaseAdd BLAST217

Structurei

3D structure databases

ProteinModelPortaliP02963.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini21 – 211NodB homologyPROSITE-ProRule annotationAdd BLAST191

Sequence similaritiesi

Belongs to the polysaccharide deacetylase family.Curated
Contains 1 NodB homology domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000260719.
KOiK14659.
OMAiIRAPYGI.

Family and domain databases

Gene3Di3.20.20.370. 1 hit.
InterProiIPR011330. Glyco_hydro/deAcase_b/a-brl.
IPR002509. NODB_dom.
IPR026402. Nodulat_NodB.
[Graphical view]
PfamiPF01522. Polysacc_deac_1. 1 hit.
[Graphical view]
SUPFAMiSSF88713. SSF88713. 1 hit.
TIGRFAMsiTIGR04243. nodulat_NodB. 1 hit.
PROSITEiPS51677. NODB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P02963-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKHLDYIHEV PSNCDYGTED RSIYLTFDDG PNPHCTPEIL DVLAEYGVPA
60 70 80 90 100
TFFVIGTYAK SQPELIRRIV AEGHEVANHT MTHPDLSTCG PHEVEREIVE
110 120 130 140 150
ASEAIIAACP QAAVRHIRAP YGVWSEEALT RSASAGLTAI HWSADPRDWS
160 170 180 190 200
RPGANAIVDA VLDSVRPGAI VLLHDGCPPD ESGALTGLRD QTLMALSRIV
210
PALHERGFAI RPLPPHH
Length:217
Mass (Da):23,672
Last modified:November 2, 2001 - v3
Checksum:i01F82A0C75EA662D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti108 – 118ACPQAAVRHIR → LVLRPRSDTYE in AAA98361 (PubMed:4006668).CuratedAdd BLAST11

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti10V → M in strain: 41. 1
Natural varianti59A → T in strain: 41. 1
Natural varianti116H → R in strain: 41. 1
Natural varianti195 – 198ALSR → GFPV in strain: O42B. 4

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M11268 Genomic DNA. Translation: AAA98361.1.
X01649 Genomic DNA. Translation: CAA25809.1.
AF038577 Genomic DNA. Translation: AAB95330.1.
AE006469 Genomic DNA. Translation: AAK65132.1.
PIRiA03483. ZZZRBM.
A03484. ZZZRB4.
B95321.
RefSeqiNP_435720.1. NC_003037.1.
WP_003532851.1. NC_003037.1.

Genome annotation databases

EnsemblBacteriaiAAK65132; AAK65132; SMa0868.
GeneIDi1235510.
25011385.
KEGGisme:SMa0868.
PATRICi23627412. VBISinMel96828_0485.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M11268 Genomic DNA. Translation: AAA98361.1.
X01649 Genomic DNA. Translation: CAA25809.1.
AF038577 Genomic DNA. Translation: AAB95330.1.
AE006469 Genomic DNA. Translation: AAK65132.1.
PIRiA03483. ZZZRBM.
A03484. ZZZRB4.
B95321.
RefSeqiNP_435720.1. NC_003037.1.
WP_003532851.1. NC_003037.1.

3D structure databases

ProteinModelPortaliP02963.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK65132; AAK65132; SMa0868.
GeneIDi1235510.
25011385.
KEGGisme:SMa0868.
PATRICi23627412. VBISinMel96828_0485.

Phylogenomic databases

HOGENOMiHOG000260719.
KOiK14659.
OMAiIRAPYGI.

Family and domain databases

Gene3Di3.20.20.370. 1 hit.
InterProiIPR011330. Glyco_hydro/deAcase_b/a-brl.
IPR002509. NODB_dom.
IPR026402. Nodulat_NodB.
[Graphical view]
PfamiPF01522. Polysacc_deac_1. 1 hit.
[Graphical view]
SUPFAMiSSF88713. SSF88713. 1 hit.
TIGRFAMsiTIGR04243. nodulat_NodB. 1 hit.
PROSITEiPS51677. NODB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNODB_RHIME
AccessioniPrimary (citable) accession number: P02963
Secondary accession number(s): O52477
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: November 2, 2001
Last modified: November 2, 2016
This is version 108 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Plasmid, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.