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Protein

Outer membrane pore protein E

Gene

phoE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Uptake of inorganic phosphate, phosphorylated compounds, and some other negatively charged solutes.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Porin

Keywords - Biological processi

Ion transport, Stress response, Transport

Enzyme and pathway databases

BioCyciEcoCyc:MONOMER0-282.
ECOL316407:JW0231-MONOMER.

Protein family/group databases

TCDBi1.B.1.1.2. the general bacterial porin (gbp) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Outer membrane pore protein E
Gene namesi
Name:phoE
Synonyms:ompE
Ordered Locus Names:b0241, JW0231
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10729. phoE.

Subcellular locationi

GO - Cellular componenti

  • cell outer membrane Source: UniProtKB-SubCell
  • pore complex Source: CACAO
Complete GO annotation...

Keywords - Cellular componenti

Cell outer membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi351F → Y, N, S or V: Less resistant to trypsin. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Add BLAST21
ChainiPRO_000002524222 – 351Outer membrane pore protein EAdd BLAST330

Proteomic databases

PaxDbiP02932.
PRIDEiP02932.

2D gel databases

SWISS-2DPAGEP02932.

Expressioni

Inductioni

By phosphate starvation.

Interactioni

Subunit structurei

Homotrimer.

Protein-protein interaction databases

BioGridi4259766. 263 interactors.
DIPiDIP-10498N.
IntActiP02932. 4 interactors.
MINTiMINT-1285942.
STRINGi511145.b0241.

Structurei

Secondary structure

1351
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi28 – 49Combined sources22
Beta strandi56 – 65Combined sources10
Beta strandi68 – 85Combined sources18
Beta strandi93 – 104Combined sources12
Turni105 – 107Combined sources3
Beta strandi108 – 116Combined sources9
Helixi120 – 123Combined sources4
Turni124 – 126Combined sources3
Beta strandi129 – 131Combined sources3
Turni143 – 145Combined sources3
Beta strandi146 – 158Combined sources13
Turni159 – 162Combined sources4
Beta strandi166 – 173Combined sources8
Beta strandi187 – 197Combined sources11
Turni198 – 201Combined sources4
Beta strandi202 – 212Combined sources11
Helixi215 – 217Combined sources3
Beta strandi220 – 223Combined sources4
Beta strandi225 – 239Combined sources15
Beta strandi242 – 253Combined sources12
Beta strandi258 – 261Combined sources4
Beta strandi263 – 274Combined sources12
Beta strandi277 – 294Combined sources18
Turni295 – 297Combined sources3
Beta strandi301 – 327Combined sources27
Beta strandi342 – 351Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1PHOX-ray3.00A22-351[»]
ProteinModelPortaliP02932.
SMRiP02932.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP02932.

Family & Domainsi

Sequence similaritiesi

Belongs to the Gram-negative porin family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane beta strand

Phylogenomic databases

eggNOGiENOG4105FCK. Bacteria.
COG3203. LUCA.
HOGENOMiHOG000272406.
InParanoidiP02932.
KOiK11929.
OMAiQWEYQYN.
PhylomeDBiP02932.

Family and domain databases

CDDicd00342. gram_neg_porins. 1 hit.
Gene3Di2.40.160.10. 1 hit.
InterProiIPR033900. Gram_neg_porin_domain.
IPR023614. Porin_dom.
IPR001897. Porin_gammaproteobac.
IPR001702. Porin_Gram-ve.
IPR013793. Porin_Gram-ve_CS.
[Graphical view]
PfamiPF00267. Porin_1. 1 hit.
[Graphical view]
PRINTSiPR00183. ECOLIPORIN.
PR00182. ECOLNEIPORIN.
PROSITEiPS00576. GRAM_NEG_PORIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P02932-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKSTLALVV MGIVASASVQ AAEIYNKDGN KLDVYGKVKA MHYMSDNASK
60 70 80 90 100
DGDQSYIRFG FKGETQINDQ LTGYGRWEAE FAGNKAESDT AQQKTRLAFA
110 120 130 140 150
GLKYKDLGSF DYGRNLGALY DVEAWTDMFP EFGGDSSAQT DNFMTKRASG
160 170 180 190 200
LATYRNTDFF GVIDGLNLTL QYQGKNENRD VKKQNGDGFG TSLTYDFGGS
210 220 230 240 250
DFAISGAYTN SDRTNEQNLQ SRGTGKRAEA WATGLKYDAN NIYLATFYSE
260 270 280 290 300
TRKMTPITGG FANKTQNFEA VAQYQFDFGL RPSLGYVLSK GKDIEGIGDE
310 320 330 340 350
DLVNYIDVGA TYYFNKNMSA FVDYKINQLD SDNKLNINND DIVAVGMTYQ

F
Length:351
Mass (Da):38,922
Last modified:July 21, 1986 - v1
Checksum:i249E2E362C030C9A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00316 Genomic DNA. Translation: CAA23605.1.
U70214 Genomic DNA. Translation: AAB08661.1.
U00096 Genomic DNA. Translation: AAC73345.1.
AP009048 Genomic DNA. Translation: BAA77910.1.
X00786 Genomic DNA. Translation: CAA25362.1.
PIRiA03432. MMECPE.
RefSeqiNP_414776.1. NC_000913.3.
WP_000749863.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73345; AAC73345; b0241.
BAA77910; BAA77910; BAA77910.
GeneIDi944926.
KEGGiecj:JW0231.
eco:b0241.
PATRICi32115597. VBIEscCol129921_0243.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00316 Genomic DNA. Translation: CAA23605.1.
U70214 Genomic DNA. Translation: AAB08661.1.
U00096 Genomic DNA. Translation: AAC73345.1.
AP009048 Genomic DNA. Translation: BAA77910.1.
X00786 Genomic DNA. Translation: CAA25362.1.
PIRiA03432. MMECPE.
RefSeqiNP_414776.1. NC_000913.3.
WP_000749863.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1PHOX-ray3.00A22-351[»]
ProteinModelPortaliP02932.
SMRiP02932.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259766. 263 interactors.
DIPiDIP-10498N.
IntActiP02932. 4 interactors.
MINTiMINT-1285942.
STRINGi511145.b0241.

Protein family/group databases

TCDBi1.B.1.1.2. the general bacterial porin (gbp) family.

2D gel databases

SWISS-2DPAGEP02932.

Proteomic databases

PaxDbiP02932.
PRIDEiP02932.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73345; AAC73345; b0241.
BAA77910; BAA77910; BAA77910.
GeneIDi944926.
KEGGiecj:JW0231.
eco:b0241.
PATRICi32115597. VBIEscCol129921_0243.

Organism-specific databases

EchoBASEiEB0722.
EcoGeneiEG10729. phoE.

Phylogenomic databases

eggNOGiENOG4105FCK. Bacteria.
COG3203. LUCA.
HOGENOMiHOG000272406.
InParanoidiP02932.
KOiK11929.
OMAiQWEYQYN.
PhylomeDBiP02932.

Enzyme and pathway databases

BioCyciEcoCyc:MONOMER0-282.
ECOL316407:JW0231-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP02932.
PROiP02932.

Family and domain databases

CDDicd00342. gram_neg_porins. 1 hit.
Gene3Di2.40.160.10. 1 hit.
InterProiIPR033900. Gram_neg_porin_domain.
IPR023614. Porin_dom.
IPR001897. Porin_gammaproteobac.
IPR001702. Porin_Gram-ve.
IPR013793. Porin_Gram-ve_CS.
[Graphical view]
PfamiPF00267. Porin_1. 1 hit.
[Graphical view]
PRINTSiPR00183. ECOLIPORIN.
PR00182. ECOLNEIPORIN.
PROSITEiPS00576. GRAM_NEG_PORIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPHOE_ECOLI
AccessioniPrimary (citable) accession number: P02932
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: November 30, 2016
This is version 157 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.