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Protein

Outer membrane pore protein E

Gene

phoE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Uptake of inorganic phosphate, phosphorylated compounds, and some other negatively charged solutes.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Porin

Keywords - Biological processi

Ion transport, Stress response, Transport

Enzyme and pathway databases

BioCyciEcoCyc:MONOMER0-282.
ECOL316407:JW0231-MONOMER.

Protein family/group databases

TCDBi1.B.1.1.2. the general bacterial porin (gbp) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Outer membrane pore protein E
Gene namesi
Name:phoE
Synonyms:ompE
Ordered Locus Names:b0241, JW0231
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10729. phoE.

Subcellular locationi

GO - Cellular componenti

  • cell outer membrane Source: UniProtKB-SubCell
  • pore complex Source: CACAO
Complete GO annotation...

Keywords - Cellular componenti

Cell outer membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi351 – 3511F → Y, N, S or V: Less resistant to trypsin. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Add
BLAST
Chaini22 – 351330Outer membrane pore protein EPRO_0000025242Add
BLAST

Proteomic databases

PaxDbiP02932.
PRIDEiP02932.

2D gel databases

SWISS-2DPAGEP02932.

Expressioni

Inductioni

By phosphate starvation.

Interactioni

Subunit structurei

Homotrimer.

Protein-protein interaction databases

BioGridi4259766. 263 interactions.
DIPiDIP-10498N.
IntActiP02932. 4 interactions.
MINTiMINT-1285942.
STRINGi511145.b0241.

Structurei

Secondary structure

1
351
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi28 – 4922Combined sources
Beta strandi56 – 6510Combined sources
Beta strandi68 – 8518Combined sources
Beta strandi93 – 10412Combined sources
Turni105 – 1073Combined sources
Beta strandi108 – 1169Combined sources
Helixi120 – 1234Combined sources
Turni124 – 1263Combined sources
Beta strandi129 – 1313Combined sources
Turni143 – 1453Combined sources
Beta strandi146 – 15813Combined sources
Turni159 – 1624Combined sources
Beta strandi166 – 1738Combined sources
Beta strandi187 – 19711Combined sources
Turni198 – 2014Combined sources
Beta strandi202 – 21211Combined sources
Helixi215 – 2173Combined sources
Beta strandi220 – 2234Combined sources
Beta strandi225 – 23915Combined sources
Beta strandi242 – 25312Combined sources
Beta strandi258 – 2614Combined sources
Beta strandi263 – 27412Combined sources
Beta strandi277 – 29418Combined sources
Turni295 – 2973Combined sources
Beta strandi301 – 32727Combined sources
Beta strandi342 – 35110Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1PHOX-ray3.00A22-351[»]
ProteinModelPortaliP02932.
SMRiP02932. Positions 22-351.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP02932.

Family & Domainsi

Sequence similaritiesi

Belongs to the Gram-negative porin family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane beta strand

Phylogenomic databases

eggNOGiENOG4105FCK. Bacteria.
COG3203. LUCA.
HOGENOMiHOG000272406.
InParanoidiP02932.
KOiK11929.
OMAiQWEYQYN.
PhylomeDBiP02932.

Family and domain databases

Gene3Di2.40.160.10. 1 hit.
InterProiIPR023614. Porin_dom.
IPR001897. Porin_gammaproteobac.
IPR001702. Porin_Gram-ve.
IPR013793. Porin_Gram-ve_CS.
[Graphical view]
PfamiPF00267. Porin_1. 1 hit.
[Graphical view]
PRINTSiPR00183. ECOLIPORIN.
PR00182. ECOLNEIPORIN.
PROSITEiPS00576. GRAM_NEG_PORIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P02932-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKSTLALVV MGIVASASVQ AAEIYNKDGN KLDVYGKVKA MHYMSDNASK
60 70 80 90 100
DGDQSYIRFG FKGETQINDQ LTGYGRWEAE FAGNKAESDT AQQKTRLAFA
110 120 130 140 150
GLKYKDLGSF DYGRNLGALY DVEAWTDMFP EFGGDSSAQT DNFMTKRASG
160 170 180 190 200
LATYRNTDFF GVIDGLNLTL QYQGKNENRD VKKQNGDGFG TSLTYDFGGS
210 220 230 240 250
DFAISGAYTN SDRTNEQNLQ SRGTGKRAEA WATGLKYDAN NIYLATFYSE
260 270 280 290 300
TRKMTPITGG FANKTQNFEA VAQYQFDFGL RPSLGYVLSK GKDIEGIGDE
310 320 330 340 350
DLVNYIDVGA TYYFNKNMSA FVDYKINQLD SDNKLNINND DIVAVGMTYQ

F
Length:351
Mass (Da):38,922
Last modified:July 21, 1986 - v1
Checksum:i249E2E362C030C9A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00316 Genomic DNA. Translation: CAA23605.1.
U70214 Genomic DNA. Translation: AAB08661.1.
U00096 Genomic DNA. Translation: AAC73345.1.
AP009048 Genomic DNA. Translation: BAA77910.1.
X00786 Genomic DNA. Translation: CAA25362.1.
PIRiA03432. MMECPE.
RefSeqiNP_414776.1. NC_000913.3.
WP_000749863.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73345; AAC73345; b0241.
BAA77910; BAA77910; BAA77910.
GeneIDi944926.
KEGGiecj:JW0231.
eco:b0241.
PATRICi32115597. VBIEscCol129921_0243.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00316 Genomic DNA. Translation: CAA23605.1.
U70214 Genomic DNA. Translation: AAB08661.1.
U00096 Genomic DNA. Translation: AAC73345.1.
AP009048 Genomic DNA. Translation: BAA77910.1.
X00786 Genomic DNA. Translation: CAA25362.1.
PIRiA03432. MMECPE.
RefSeqiNP_414776.1. NC_000913.3.
WP_000749863.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1PHOX-ray3.00A22-351[»]
ProteinModelPortaliP02932.
SMRiP02932. Positions 22-351.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259766. 263 interactions.
DIPiDIP-10498N.
IntActiP02932. 4 interactions.
MINTiMINT-1285942.
STRINGi511145.b0241.

Protein family/group databases

TCDBi1.B.1.1.2. the general bacterial porin (gbp) family.

2D gel databases

SWISS-2DPAGEP02932.

Proteomic databases

PaxDbiP02932.
PRIDEiP02932.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73345; AAC73345; b0241.
BAA77910; BAA77910; BAA77910.
GeneIDi944926.
KEGGiecj:JW0231.
eco:b0241.
PATRICi32115597. VBIEscCol129921_0243.

Organism-specific databases

EchoBASEiEB0722.
EcoGeneiEG10729. phoE.

Phylogenomic databases

eggNOGiENOG4105FCK. Bacteria.
COG3203. LUCA.
HOGENOMiHOG000272406.
InParanoidiP02932.
KOiK11929.
OMAiQWEYQYN.
PhylomeDBiP02932.

Enzyme and pathway databases

BioCyciEcoCyc:MONOMER0-282.
ECOL316407:JW0231-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP02932.
PROiP02932.

Family and domain databases

Gene3Di2.40.160.10. 1 hit.
InterProiIPR023614. Porin_dom.
IPR001897. Porin_gammaproteobac.
IPR001702. Porin_Gram-ve.
IPR013793. Porin_Gram-ve_CS.
[Graphical view]
PfamiPF00267. Porin_1. 1 hit.
[Graphical view]
PRINTSiPR00183. ECOLIPORIN.
PR00182. ECOLNEIPORIN.
PROSITEiPS00576. GRAM_NEG_PORIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPHOE_ECOLI
AccessioniPrimary (citable) accession number: P02932
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: September 7, 2016
This is version 155 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.