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Protein

Outer membrane protein TolC

Gene

tolC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Outer membrane channel, which is required for the function of several efflux systems such as AcrAB-TolC, AcrEF-TolC, EmrAB-TolC and MacAB-TolC. These systems are involved in export of antibiotics and other toxic compounds from the cell. TolC is also involved in import of colicin E1 into the cells.5 Publications

Enzyme regulationi

In vitro, inhibited by hexaamminecobalt3+.1 Publication

GO - Molecular functioni

  • efflux transmembrane transporter activity Source: EcoCyc
  • porin activity Source: EcoCyc

GO - Biological processi

  • enterobactin transport Source: EcoliWiki
  • response to antibiotic Source: UniProtKB-KW
  • response to organic cyclic compound Source: EcoliWiki
Complete GO annotation...

Keywords - Biological processi

Antibiotic resistance, Transport

Enzyme and pathway databases

BioCyciEcoCyc:EG11009-MONOMER.
ECOL316407:JW5503-MONOMER.
MetaCyc:EG11009-MONOMER.

Protein family/group databases

TCDBi1.B.17.1.1. the outer membrane factor (omf) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Outer membrane protein TolC
Alternative name(s):
Multidrug efflux pump subunit TolC
Outer membrane factor TolC
Gene namesi
Name:tolC
Synonyms:colE1-i, mtcB, mukA, refI, toc, weeA
Ordered Locus Names:b3035, JW5503
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11009. tolC.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini23 – 62PeriplasmicAdd BLAST40
Transmembranei63 – 74Beta stranded; Name=S1Add BLAST12
Topological domaini75 – 82Extracellular8
Transmembranei83 – 96Beta stranded; Name=S2Add BLAST14
Topological domaini97 – 268PeriplasmicAdd BLAST172
Transmembranei269 – 279Beta stranded; Name=S4Add BLAST11
Topological domaini280 – 300ExtracellularAdd BLAST21
Transmembranei301 – 311Beta stranded; Name=S5Add BLAST11
Topological domaini312 – 493PeriplasmicAdd BLAST182

GO - Cellular componenti

  • cell outer membrane Source: EcoCyc
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell outer membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Cannot grow on efflux substrates novobiocin or fusidic acid.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi384Y → F: Partial channel opening. Increases sensitivity to vancomycin, by allowing its passive diffusion across the outer membrane; when associated with E-389. 1 Publication1
Mutagenesisi389R → E: Partial channel opening. Increases sensitivity to vancomycin, by allowing its passive diffusion across the outer membrane; when associated with F-382. 1 Publication1
Mutagenesisi393D → A: Decreases inhibition by hexaamminecobalt(3+). 1 Publication1
Mutagenesisi396D → A: Decreases inhibition by hexaamminecobalt(3+). 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 223 PublicationsAdd BLAST22
ChainiPRO_000001335223 – 493Outer membrane protein TolCAdd BLAST471

Proteomic databases

EPDiP02930.
PaxDbiP02930.
PRIDEiP02930.

2D gel databases

SWISS-2DPAGEP02930.

Interactioni

Subunit structurei

Homotrimer. Part of tripartite efflux systems, which are composed of an inner membrane transporter, a periplasmic membrane fusion protein, and an outer membrane component, TolC. The complexes form a large protein conduit and can translocate molecules across both the inner and outer membranes. TolC interacts with the membrane fusion proteins AcrA, EmrA and MacA.8 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-875614,EBI-875614

Protein-protein interaction databases

BioGridi4263248. 380 interactors.
DIPiDIP-11007N.
IntActiP02930. 5 interactors.
STRINGi511145.b3035.

Structurei

Secondary structure

1493
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi25 – 35Combined sources11
Helixi37 – 57Combined sources21
Helixi58 – 60Combined sources3
Beta strandi63 – 75Combined sources13
Beta strandi77 – 79Combined sources3
Beta strandi83 – 98Combined sources16
Helixi100 – 167Combined sources68
Beta strandi168 – 170Combined sources3
Helixi173 – 208Combined sources36
Beta strandi213 – 218Combined sources6
Turni220 – 222Combined sources3
Helixi231 – 241Combined sources11
Helixi243 – 263Combined sources21
Helixi264 – 266Combined sources3
Beta strandi269 – 279Combined sources11
Beta strandi282 – 285Combined sources4
Beta strandi288 – 291Combined sources4
Beta strandi293 – 297Combined sources5
Beta strandi302 – 316Combined sources15
Helixi319 – 385Combined sources67
Beta strandi386 – 388Combined sources3
Helixi391 – 426Combined sources36
Helixi431 – 439Combined sources9
Beta strandi441 – 448Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1EK9X-ray2.10A/B/C23-450[»]
1TQQX-ray2.75A/B/C23-493[»]
2VDDX-ray3.30A/B/C1-450[»]
2VDEX-ray3.20A/B/C1-450[»]
2WMZX-ray2.90A/B/C23-450[»]
2XMNX-ray2.85A/B/C23-450[»]
ProteinModelPortaliP02930.
SMRiP02930.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP02930.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati23 – 2301Add BLAST208
Repeati231 – 4462Add BLAST216

Domaini

Forms a continuous, solvent-accessible conduit: a 'channel-tunnel' over 140 Angstroms long that spans both the outer membrane and periplasmic space. The periplasmic or proximal end of the tunnel is sealed by sets of coiled helices.1 Publication

Sequence similaritiesi

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane beta strand

Phylogenomic databases

eggNOGiENOG4105DFG. Bacteria.
COG1538. LUCA.
HOGENOMiHOG000283506.
InParanoidiP02930.
KOiK12340.
OMAiAMSQAEN.
PhylomeDBiP02930.

Family and domain databases

InterProiIPR003423. OMP_efflux.
IPR010130. T1SS_OMP_TolC.
[Graphical view]
PANTHERiPTHR30026:SF3. PTHR30026:SF3. 1 hit.
PfamiPF02321. OEP. 2 hits.
[Graphical view]
TIGRFAMsiTIGR01844. type_I_sec_TolC. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P02930-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKLLPILIG LSLSGFSSLS QAENLMQVYQ QARLSNPELR KSAADRDAAF
60 70 80 90 100
EKINEARSPL LPQLGLGADY TYSNGYRDAN GINSNATSAS LQLTQSIFDM
110 120 130 140 150
SKWRALTLQE KAAGIQDVTY QTDQQTLILN TATAYFNVLN AIDVLSYTQA
160 170 180 190 200
QKEAIYRQLD QTTQRFNVGL VAITDVQNAR AQYDTVLANE VTARNNLDNA
210 220 230 240 250
VEQLRQITGN YYPELAALNV ENFKTDKPQP VNALLKEAEK RNLSLLQARL
260 270 280 290 300
SQDLAREQIR QAQDGHLPTL DLTASTGISD TSYSGSKTRG AAGTQYDDSN
310 320 330 340 350
MGQNKVGLSF SLPIYQGGMV NSQVKQAQYN FVGASEQLES AHRSVVQTVR
360 370 380 390 400
SSFNNINASI SSINAYKQAV VSAQSSLDAM EAGYSVGTRT IVDVLDATTT
410 420 430 440 450
LYNAKQELAN ARYNYLINQL NIKSALGTLN EQDLLALNNA LSKPVSTNPE
460 470 480 490
NVAPQTPEQN AIADGYAPDS PAPVVQQTSA RTTTSNGHNP FRN
Length:493
Mass (Da):53,741
Last modified:June 27, 2006 - v3
Checksum:i6F97B4C62A848FE1
GO

Sequence cautioni

The sequence AAA69203 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence CAA24751 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence CAA37982 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti178N → K in CAA24914 (PubMed:6312426).Curated1
Sequence conflicti191V → L in CAA37982 (PubMed:2216730).Curated1
Sequence conflicti203 – 204QL → HV in CAA24914 (PubMed:6312426).Curated2
Sequence conflicti214 – 215EL → GT in CAA24914 (PubMed:6312426).Curated2
Sequence conflicti258 – 270QIRQA…HLPTL → KFARRRMVTYRLW in CAA24914 (PubMed:6312426).CuratedAdd BLAST13
Sequence conflicti278 – 295ISDTS…AAGTQ → FLTPLIAVRKPCAAVP in CAA24914 (PubMed:6312426).CuratedAdd BLAST18
Sequence conflicti325K → T in CAA24914 (PubMed:6312426).Curated1
Sequence conflicti335 – 354SEQLE…RSSFN → ASTWKVPIVASCQRAFCFS in CAA24914 (PubMed:6312426).CuratedAdd BLAST20
Sequence conflicti365 – 370AYKQAV → RYTQAA in CAA24914 (PubMed:6312426).Curated6
Sequence conflicti400 – 411TLYNA…ELANA → SCTAQARAGNP in CAA24914 (PubMed:6312426).CuratedAdd BLAST12
Sequence conflicti445V → I in CAA24914 (PubMed:6312426).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X00016 Genomic DNA. Translation: CAA24914.1.
X54049 Genomic DNA. Translation: CAA37982.1. Different initiation.
U28377 Genomic DNA. Translation: AAA69203.1. Different initiation.
U00096 Genomic DNA. Translation: AAC76071.2.
AP009048 Genomic DNA. Translation: BAE77091.1.
V01505 Genomic DNA. Translation: CAA24751.1. Different initiation.
PIRiA65091. MMECTC.
RefSeqiNP_417507.2. NC_000913.3.
WP_000735278.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76071; AAC76071; b3035.
BAE77091; BAE77091; BAE77091.
GeneIDi947521.
KEGGiecj:JW5503.
eco:b3035.
PATRICi32121480. VBIEscCol129921_3127.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X00016 Genomic DNA. Translation: CAA24914.1.
X54049 Genomic DNA. Translation: CAA37982.1. Different initiation.
U28377 Genomic DNA. Translation: AAA69203.1. Different initiation.
U00096 Genomic DNA. Translation: AAC76071.2.
AP009048 Genomic DNA. Translation: BAE77091.1.
V01505 Genomic DNA. Translation: CAA24751.1. Different initiation.
PIRiA65091. MMECTC.
RefSeqiNP_417507.2. NC_000913.3.
WP_000735278.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1EK9X-ray2.10A/B/C23-450[»]
1TQQX-ray2.75A/B/C23-493[»]
2VDDX-ray3.30A/B/C1-450[»]
2VDEX-ray3.20A/B/C1-450[»]
2WMZX-ray2.90A/B/C23-450[»]
2XMNX-ray2.85A/B/C23-450[»]
ProteinModelPortaliP02930.
SMRiP02930.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263248. 380 interactors.
DIPiDIP-11007N.
IntActiP02930. 5 interactors.
STRINGi511145.b3035.

Protein family/group databases

TCDBi1.B.17.1.1. the outer membrane factor (omf) family.

2D gel databases

SWISS-2DPAGEP02930.

Proteomic databases

EPDiP02930.
PaxDbiP02930.
PRIDEiP02930.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76071; AAC76071; b3035.
BAE77091; BAE77091; BAE77091.
GeneIDi947521.
KEGGiecj:JW5503.
eco:b3035.
PATRICi32121480. VBIEscCol129921_3127.

Organism-specific databases

EchoBASEiEB1002.
EcoGeneiEG11009. tolC.

Phylogenomic databases

eggNOGiENOG4105DFG. Bacteria.
COG1538. LUCA.
HOGENOMiHOG000283506.
InParanoidiP02930.
KOiK12340.
OMAiAMSQAEN.
PhylomeDBiP02930.

Enzyme and pathway databases

BioCyciEcoCyc:EG11009-MONOMER.
ECOL316407:JW5503-MONOMER.
MetaCyc:EG11009-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP02930.
PROiP02930.

Family and domain databases

InterProiIPR003423. OMP_efflux.
IPR010130. T1SS_OMP_TolC.
[Graphical view]
PANTHERiPTHR30026:SF3. PTHR30026:SF3. 1 hit.
PfamiPF02321. OEP. 2 hits.
[Graphical view]
TIGRFAMsiTIGR01844. type_I_sec_TolC. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTOLC_ECOLI
AccessioniPrimary (citable) accession number: P02930
Secondary accession number(s): Q2M9G5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: June 27, 2006
Last modified: November 2, 2016
This is version 162 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.