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Protein

L-arabinose-binding periplasmic protein

Gene

araF

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the high-affinity L-arabinose membrane transport system. Binds with high affinity to arabinose, but can also bind D-galactose (approximately 2-fold reduction) and D-fucose (approximately 40-fold reduction).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei87The binding site for the sugar molecule has not yet been established, but C-87 may be involved1

GO - Molecular functioni

  • monosaccharide binding Source: EcoCyc
  • monosaccharide-transporting ATPase activity Source: EcoCyc

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Sugar transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:ARAF-MONOMER.
ECOL316407:JW1889-MONOMER.
MetaCyc:ARAF-MONOMER.

Protein family/group databases

TCDBi3.A.1.2.2. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
L-arabinose-binding periplasmic protein
Short name:
ABP
Gene namesi
Name:araF
Ordered Locus Names:b1901, JW1889
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10057. araF.

Subcellular locationi

GO - Cellular componenti

  • outer membrane-bounded periplasmic space Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi277P → G: Improves binding to galactose. 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 232 PublicationsAdd BLAST23
ChainiPRO_000003172024 – 329L-arabinose-binding periplasmic proteinAdd BLAST306

Proteomic databases

EPDiP02924.
PaxDbiP02924.
PRIDEiP02924.

Interactioni

Protein-protein interaction databases

BioGridi4261850. 13 interactors.
IntActiP02924. 5 interactors.
STRINGi511145.b1901.

Structurei

Secondary structure

1329
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi26 – 33Combined sources8
Helixi38 – 54Combined sources17
Beta strandi56 – 62Combined sources7
Helixi66 – 78Combined sources13
Beta strandi83 – 87Combined sources5
Helixi91 – 93Combined sources3
Helixi94 – 103Combined sources10
Beta strandi107 – 113Combined sources7
Beta strandi128 – 131Combined sources4
Helixi133 – 151Combined sources19
Helixi155 – 157Combined sources3
Beta strandi158 – 164Combined sources7
Helixi169 – 185Combined sources17
Helixi189 – 191Combined sources3
Beta strandi192 – 196Combined sources5
Beta strandi198 – 201Combined sources4
Helixi202 – 213Combined sources12
Beta strandi220 – 225Combined sources6
Helixi229 – 241Combined sources13
Helixi246 – 248Combined sources3
Beta strandi249 – 256Combined sources8
Helixi257 – 259Combined sources3
Helixi260 – 263Combined sources4
Beta strandi265 – 267Combined sources3
Beta strandi270 – 276Combined sources7
Helixi279 – 296Combined sources18
Beta strandi302 – 306Combined sources5
Beta strandi310 – 313Combined sources4
Turni314 – 316Combined sources3
Helixi317 – 323Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ABEX-ray1.70A24-329[»]
1ABFX-ray1.90A24-329[»]
1APBX-ray1.76A24-329[»]
1BAPX-ray1.75A24-329[»]
2WRZX-ray2.20A/B24-329[»]
5ABPX-ray1.80A24-329[»]
6ABPX-ray1.67A24-329[»]
7ABPX-ray1.67A24-329[»]
8ABPX-ray1.49A24-329[»]
9ABPX-ray1.97A24-329[»]
ProteinModelPortaliP02924.
SMRiP02924.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP02924.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105DMK. Bacteria.
COG1879. LUCA.
HOGENOMiHOG000220638.
KOiK10537.
OMAiNDIPGAL.
PhylomeDBiP02924.

Family and domain databases

CDDicd01540. PBP1_arabinose_binding. 1 hit.
InterProiIPR026266. AraF.
IPR001761. Peripla_BP/Lac1_sug-bd_dom.
IPR028082. Peripla_BP_I.
[Graphical view]
PfamiPF00532. Peripla_BP_1. 1 hit.
[Graphical view]
PIRSFiPIRSF002816. AraF. 1 hit.
SUPFAMiSSF53822. SSF53822. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P02924-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHKFTKALAA IGLAAVMSQS AMAENLKLGF LVKQPEEPWF QTEWKFADKA
60 70 80 90 100
GKDLGFEVIK IAVPDGEKTL NAIDSLAASG AKGFVICTPD PKLGSAIVAK
110 120 130 140 150
ARGYDMKVIA VDDQFVNAKG KPMDTVPLVM MAATKIGERQ GQELYKEMQK
160 170 180 190 200
RGWDVKESAV MAITANELDT ARRRTTGSMD ALKAAGFPEK QIYQVPTKSN
210 220 230 240 250
DIPGAFDAAN SMLVQHPEVK HWLIVGMNDS TVLGGVRATE GQGFKAADII
260 270 280 290 300
GIGINGVDAV SELSKAQATG FYGSLLPSPD VHGYKSSEML YNWVAKDVEP
310 320
PKFTEVTDVV LITRDNFKEE LEKKGLGGK
Length:329
Mass (Da):35,541
Last modified:November 1, 1997 - v2
Checksum:iA79D167EA92B0C64
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti194Q → E in CAA29476 (PubMed:2445996).Curated1
Sequence conflicti281V → L in CAA29476 (PubMed:2445996).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06091 Genomic DNA. Translation: CAA29476.1.
U00096 Genomic DNA. Translation: AAC74971.1.
AP009048 Genomic DNA. Translation: BAA15721.1.
K00420 Genomic DNA. Translation: AAA23472.1.
PIRiE64953. JGECA.
RefSeqiNP_416414.1. NC_000913.3.
WP_000548675.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74971; AAC74971; b1901.
BAA15721; BAA15721; BAA15721.
GeneIDi946409.
KEGGiecj:JW1889.
eco:b1901.
PATRICi32119127. VBIEscCol129921_1981.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06091 Genomic DNA. Translation: CAA29476.1.
U00096 Genomic DNA. Translation: AAC74971.1.
AP009048 Genomic DNA. Translation: BAA15721.1.
K00420 Genomic DNA. Translation: AAA23472.1.
PIRiE64953. JGECA.
RefSeqiNP_416414.1. NC_000913.3.
WP_000548675.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ABEX-ray1.70A24-329[»]
1ABFX-ray1.90A24-329[»]
1APBX-ray1.76A24-329[»]
1BAPX-ray1.75A24-329[»]
2WRZX-ray2.20A/B24-329[»]
5ABPX-ray1.80A24-329[»]
6ABPX-ray1.67A24-329[»]
7ABPX-ray1.67A24-329[»]
8ABPX-ray1.49A24-329[»]
9ABPX-ray1.97A24-329[»]
ProteinModelPortaliP02924.
SMRiP02924.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261850. 13 interactors.
IntActiP02924. 5 interactors.
STRINGi511145.b1901.

Protein family/group databases

TCDBi3.A.1.2.2. the atp-binding cassette (abc) superfamily.

Proteomic databases

EPDiP02924.
PaxDbiP02924.
PRIDEiP02924.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74971; AAC74971; b1901.
BAA15721; BAA15721; BAA15721.
GeneIDi946409.
KEGGiecj:JW1889.
eco:b1901.
PATRICi32119127. VBIEscCol129921_1981.

Organism-specific databases

EchoBASEiEB0055.
EcoGeneiEG10057. araF.

Phylogenomic databases

eggNOGiENOG4105DMK. Bacteria.
COG1879. LUCA.
HOGENOMiHOG000220638.
KOiK10537.
OMAiNDIPGAL.
PhylomeDBiP02924.

Enzyme and pathway databases

BioCyciEcoCyc:ARAF-MONOMER.
ECOL316407:JW1889-MONOMER.
MetaCyc:ARAF-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP02924.
PROiP02924.

Family and domain databases

CDDicd01540. PBP1_arabinose_binding. 1 hit.
InterProiIPR026266. AraF.
IPR001761. Peripla_BP/Lac1_sug-bd_dom.
IPR028082. Peripla_BP_I.
[Graphical view]
PfamiPF00532. Peripla_BP_1. 1 hit.
[Graphical view]
PIRSFiPIRSF002816. AraF. 1 hit.
SUPFAMiSSF53822. SSF53822. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiARAF_ECOLI
AccessioniPrimary (citable) accession number: P02924
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 146 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.