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Protein

Melibiose carrier protein

Gene

melB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Responsible for melibiose and other galactoside transport. It is capable of using hydrogen, sodium, and lithium cations as coupling cations for cotransport, depending on the particular sugar transported (symport system).

GO - Molecular functioni

  • melibiose:monovalent cation symporter activity Source: EcoCyc
  • melibiose:sodium symporter activity Source: EcoCyc
  • methylgalactoside transmembrane transporter activity Source: EcoCyc

GO - Biological processi

  • melibiose transport Source: EcoCyc
  • methylgalactoside transport Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Sugar transport, Symport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:MELB-MONOMER.
ECOL316407:JW4081-MONOMER.
MetaCyc:MELB-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Melibiose carrier protein
Alternative name(s):
Melibiose permease
Melibiose transporter
Na+ (Li+)/melibiose symporter
Thiomethylgalactoside permease II
Gene namesi
Name:melB
Synonyms:mel-4
Ordered Locus Names:b4120, JW4081
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10578. melB.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini5 – 19CytoplasmicCuratedAdd BLAST15
Transmembranei20 – 40HelicalCuratedAdd BLAST21
Topological domaini41 – 42PeriplasmicCurated2
Transmembranei43 – 63HelicalCuratedAdd BLAST21
Topological domaini64 – 80CytoplasmicCuratedAdd BLAST17
Transmembranei81 – 101HelicalCuratedAdd BLAST21
Topological domaini102 – 108PeriplasmicCurated7
Transmembranei109 – 129HelicalCuratedAdd BLAST21
Topological domaini130 – 153CytoplasmicCuratedAdd BLAST24
Transmembranei154 – 174HelicalCuratedAdd BLAST21
Topological domaini175 – 186PeriplasmicCuratedAdd BLAST12
Transmembranei187 – 207HelicalCuratedAdd BLAST21
Topological domaini208 – 235CytoplasmicCuratedAdd BLAST28
Transmembranei236 – 256HelicalCuratedAdd BLAST21
Topological domaini257 – 269PeriplasmicCuratedAdd BLAST13
Transmembranei270 – 290HelicalCuratedAdd BLAST21
Topological domaini291 – 299CytoplasmicCurated9
Transmembranei300 – 320HelicalCuratedAdd BLAST21
Topological domaini321 – 328PeriplasmicCurated8
Transmembranei329 – 349HelicalCuratedAdd BLAST21
Topological domaini350 – 381CytoplasmicCuratedAdd BLAST32
Transmembranei382 – 402HelicalCuratedAdd BLAST21
Topological domaini403 – 410PeriplasmicCurated8
Transmembranei411 – 431HelicalCuratedAdd BLAST21
Topological domaini432 – 473CytoplasmicCuratedAdd BLAST42

GO - Cellular componenti

  • integral component of plasma membrane Source: EcoCyc
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi59D → C: Loses ability to catalyze Na(+) or H(+)-coupled melibiose transport against a concentration gradient. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001707522 – 473Melibiose carrier proteinAdd BLAST472

Proteomic databases

PaxDbiP02921.
PRIDEiP02921.

Interactioni

Subunit structurei

Monomer and homodimer.1 Publication

Protein-protein interaction databases

BioGridi4263176. 14 interactors.
DIPiDIP-10180N.
IntActiP02921. 1 interactor.
MINTiMINT-1320140.
STRINGi511145.b4120.

Structurei

3D structure databases

ProteinModelPortaliP02921.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105CT4. Bacteria.
COG2211. LUCA.
HOGENOMiHOG000294602.
InParanoidiP02921.
KOiK11104.

Family and domain databases

InterProiIPR020846. MFS_dom.
IPR001927. Na/Gal_symport.
IPR018043. Na/Gal_symport_CS.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
TIGRFAMsiTIGR00792. gph. 1 hit.
PROSITEiPS00872. NA_GALACTOSIDE_SYMP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P02921-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSISMTTKLS YGFGAFGKDF AIGIVYMYLM YYYTDVVGLS VGLVGTLFLV
60 70 80 90 100
ARIWDAINDP IMGWIVNATR SRWGKFKPWI LIGTLANSVI LFLLFSAHLF
110 120 130 140 150
EGTTQIVFVC VTYILWGMTY TIMDIPFWSL VPTITLDKRE REQLVPYPRF
160 170 180 190 200
FASLAGFVTA GVTLPFVNYV GGGDRGFGFQ MFTLVLIAFF IVSTIITLRN
210 220 230 240 250
VHEVFSSDNQ PSAEGSHLTL KAIVALIYKN DQLSCLLGMA LAYNVASNII
260 270 280 290 300
TGFAIYYFSY VIGDADLFPY YLSYAGAANL VTLVFFPRLV KSLSRRILWA
310 320 330 340 350
GASILPVLSC GVLLLMALMS YHNVVLIVIA GILLNVGTAL FWVLQVIMVA
360 370 380 390 400
DIVDYGEYKL HVRCESIAYS VQTMVVKGGS AFAAFFIAVV LGMIGYVPNV
410 420 430 440 450
EQSTQALLGM QFIMIALPTL FFMVTLILYF RFYRLNGDTL RRIQIHLLDK
460 470
YRKVPPEPVH ADIPVGAVSD VKA
Length:473
Mass (Da):52,636
Last modified:October 19, 2011 - v2
Checksum:iE05FAA436F4CA70C
GO

Sequence cautioni

The sequence AAA24148 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAA97020 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAE78122 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K01991 Genomic DNA. Translation: AAA24148.1. Different initiation.
U14003 Genomic DNA. Translation: AAA97020.1. Different initiation.
U00096 Genomic DNA. Translation: AAC77081.2.
AP009048 Genomic DNA. Translation: BAE78122.1. Different initiation.
PIRiA03421. BDEC.
RefSeqiNP_418544.2. NC_000913.3.
WP_000028111.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC77081; AAC77081; b4120.
BAE78122; BAE78122; BAE78122.
GeneIDi948635.
KEGGiecj:JW4081.
eco:b4120.
PATRICi32123805. VBIEscCol129921_4251.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K01991 Genomic DNA. Translation: AAA24148.1. Different initiation.
U14003 Genomic DNA. Translation: AAA97020.1. Different initiation.
U00096 Genomic DNA. Translation: AAC77081.2.
AP009048 Genomic DNA. Translation: BAE78122.1. Different initiation.
PIRiA03421. BDEC.
RefSeqiNP_418544.2. NC_000913.3.
WP_000028111.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP02921.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263176. 14 interactors.
DIPiDIP-10180N.
IntActiP02921. 1 interactor.
MINTiMINT-1320140.
STRINGi511145.b4120.

Proteomic databases

PaxDbiP02921.
PRIDEiP02921.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC77081; AAC77081; b4120.
BAE78122; BAE78122; BAE78122.
GeneIDi948635.
KEGGiecj:JW4081.
eco:b4120.
PATRICi32123805. VBIEscCol129921_4251.

Organism-specific databases

EchoBASEiEB0573.
EcoGeneiEG10578. melB.

Phylogenomic databases

eggNOGiENOG4105CT4. Bacteria.
COG2211. LUCA.
HOGENOMiHOG000294602.
InParanoidiP02921.
KOiK11104.

Enzyme and pathway databases

BioCyciEcoCyc:MELB-MONOMER.
ECOL316407:JW4081-MONOMER.
MetaCyc:MELB-MONOMER.

Miscellaneous databases

PROiP02921.

Family and domain databases

InterProiIPR020846. MFS_dom.
IPR001927. Na/Gal_symport.
IPR018043. Na/Gal_symport_CS.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
TIGRFAMsiTIGR00792. gph. 1 hit.
PROSITEiPS00872. NA_GALACTOSIDE_SYMP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMELB_ECOLI
AccessioniPrimary (citable) accession number: P02921
Secondary accession number(s): Q2M6I4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: October 19, 2011
Last modified: November 2, 2016
This is version 142 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.