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Protein

Melibiose carrier protein

Gene

melB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Responsible for melibiose and other galactoside transport. It is capable of using hydrogen, sodium, and lithium cations as coupling cations for cotransport, depending on the particular sugar transported (symport system).

GO - Molecular functioni

  • melibiose:monovalent cation symporter activity Source: EcoCyc
  • melibiose:sodium symporter activity Source: EcoCyc
  • methylgalactoside transmembrane transporter activity Source: EcoCyc

GO - Biological processi

  • melibiose transport Source: EcoCyc
  • methylgalactoside transport Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Sugar transport, Symport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:MELB-MONOMER.
ECOL316407:JW4081-MONOMER.
MetaCyc:MELB-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Melibiose carrier protein
Alternative name(s):
Melibiose permease
Melibiose transporter
Na+ (Li+)/melibiose symporter
Thiomethylgalactoside permease II
Gene namesi
Name:melB
Synonyms:mel-4
Ordered Locus Names:b4120, JW4081
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10578. melB.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini5 – 1915CytoplasmicCuratedAdd
BLAST
Transmembranei20 – 4021HelicalCuratedAdd
BLAST
Topological domaini41 – 422PeriplasmicCurated
Transmembranei43 – 6321HelicalCuratedAdd
BLAST
Topological domaini64 – 8017CytoplasmicCuratedAdd
BLAST
Transmembranei81 – 10121HelicalCuratedAdd
BLAST
Topological domaini102 – 1087PeriplasmicCurated
Transmembranei109 – 12921HelicalCuratedAdd
BLAST
Topological domaini130 – 15324CytoplasmicCuratedAdd
BLAST
Transmembranei154 – 17421HelicalCuratedAdd
BLAST
Topological domaini175 – 18612PeriplasmicCuratedAdd
BLAST
Transmembranei187 – 20721HelicalCuratedAdd
BLAST
Topological domaini208 – 23528CytoplasmicCuratedAdd
BLAST
Transmembranei236 – 25621HelicalCuratedAdd
BLAST
Topological domaini257 – 26913PeriplasmicCuratedAdd
BLAST
Transmembranei270 – 29021HelicalCuratedAdd
BLAST
Topological domaini291 – 2999CytoplasmicCurated
Transmembranei300 – 32021HelicalCuratedAdd
BLAST
Topological domaini321 – 3288PeriplasmicCurated
Transmembranei329 – 34921HelicalCuratedAdd
BLAST
Topological domaini350 – 38132CytoplasmicCuratedAdd
BLAST
Transmembranei382 – 40221HelicalCuratedAdd
BLAST
Topological domaini403 – 4108PeriplasmicCurated
Transmembranei411 – 43121HelicalCuratedAdd
BLAST
Topological domaini432 – 47342CytoplasmicCuratedAdd
BLAST

GO - Cellular componenti

  • integral component of plasma membrane Source: EcoCyc
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi59 – 591D → C: Loses ability to catalyze Na(+) or H(+)-coupled melibiose transport against a concentration gradient. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 473472Melibiose carrier proteinPRO_0000170752Add
BLAST

Proteomic databases

PaxDbiP02921.

Interactioni

Subunit structurei

Monomer and homodimer.1 Publication

Protein-protein interaction databases

BioGridi4263176. 14 interactions.
DIPiDIP-10180N.
IntActiP02921. 1 interaction.
MINTiMINT-1320140.
STRINGi511145.b4120.

Structurei

3D structure databases

ProteinModelPortaliP02921.
SMRiP02921. Positions 1-448.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105CT4. Bacteria.
COG2211. LUCA.
HOGENOMiHOG000294602.
InParanoidiP02921.
KOiK11104.

Family and domain databases

InterProiIPR020846. MFS_dom.
IPR001927. Na/Gal_symport.
IPR018043. Na/Gal_symport_CS.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
TIGRFAMsiTIGR00792. gph. 1 hit.
PROSITEiPS00872. NA_GALACTOSIDE_SYMP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P02921-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSISMTTKLS YGFGAFGKDF AIGIVYMYLM YYYTDVVGLS VGLVGTLFLV
60 70 80 90 100
ARIWDAINDP IMGWIVNATR SRWGKFKPWI LIGTLANSVI LFLLFSAHLF
110 120 130 140 150
EGTTQIVFVC VTYILWGMTY TIMDIPFWSL VPTITLDKRE REQLVPYPRF
160 170 180 190 200
FASLAGFVTA GVTLPFVNYV GGGDRGFGFQ MFTLVLIAFF IVSTIITLRN
210 220 230 240 250
VHEVFSSDNQ PSAEGSHLTL KAIVALIYKN DQLSCLLGMA LAYNVASNII
260 270 280 290 300
TGFAIYYFSY VIGDADLFPY YLSYAGAANL VTLVFFPRLV KSLSRRILWA
310 320 330 340 350
GASILPVLSC GVLLLMALMS YHNVVLIVIA GILLNVGTAL FWVLQVIMVA
360 370 380 390 400
DIVDYGEYKL HVRCESIAYS VQTMVVKGGS AFAAFFIAVV LGMIGYVPNV
410 420 430 440 450
EQSTQALLGM QFIMIALPTL FFMVTLILYF RFYRLNGDTL RRIQIHLLDK
460 470
YRKVPPEPVH ADIPVGAVSD VKA
Length:473
Mass (Da):52,636
Last modified:October 19, 2011 - v2
Checksum:iE05FAA436F4CA70C
GO

Sequence cautioni

The sequence AAA24148.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAA97020.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAE78122.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K01991 Genomic DNA. Translation: AAA24148.1. Different initiation.
U14003 Genomic DNA. Translation: AAA97020.1. Different initiation.
U00096 Genomic DNA. Translation: AAC77081.2.
AP009048 Genomic DNA. Translation: BAE78122.1. Different initiation.
PIRiA03421. BDEC.
RefSeqiNP_418544.2. NC_000913.3.
WP_000028111.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC77081; AAC77081; b4120.
BAE78122; BAE78122; BAE78122.
GeneIDi948635.
KEGGiecj:JW4081.
eco:b4120.
PATRICi32123805. VBIEscCol129921_4251.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K01991 Genomic DNA. Translation: AAA24148.1. Different initiation.
U14003 Genomic DNA. Translation: AAA97020.1. Different initiation.
U00096 Genomic DNA. Translation: AAC77081.2.
AP009048 Genomic DNA. Translation: BAE78122.1. Different initiation.
PIRiA03421. BDEC.
RefSeqiNP_418544.2. NC_000913.3.
WP_000028111.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP02921.
SMRiP02921. Positions 1-448.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263176. 14 interactions.
DIPiDIP-10180N.
IntActiP02921. 1 interaction.
MINTiMINT-1320140.
STRINGi511145.b4120.

Proteomic databases

PaxDbiP02921.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC77081; AAC77081; b4120.
BAE78122; BAE78122; BAE78122.
GeneIDi948635.
KEGGiecj:JW4081.
eco:b4120.
PATRICi32123805. VBIEscCol129921_4251.

Organism-specific databases

EchoBASEiEB0573.
EcoGeneiEG10578. melB.

Phylogenomic databases

eggNOGiENOG4105CT4. Bacteria.
COG2211. LUCA.
HOGENOMiHOG000294602.
InParanoidiP02921.
KOiK11104.

Enzyme and pathway databases

BioCyciEcoCyc:MELB-MONOMER.
ECOL316407:JW4081-MONOMER.
MetaCyc:MELB-MONOMER.

Miscellaneous databases

PROiP02921.

Family and domain databases

InterProiIPR020846. MFS_dom.
IPR001927. Na/Gal_symport.
IPR018043. Na/Gal_symport_CS.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
TIGRFAMsiTIGR00792. gph. 1 hit.
PROSITEiPS00872. NA_GALACTOSIDE_SYMP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of the melB gene and characteristics of deduced amino acid sequence of the melibiose carrier in Escherichia coli."
    Yazyu H., Shiota-Niiya S., Shimamoto T., Kanazawa H., Futai M., Tsuchiya T.
    J. Biol. Chem. 259:4320-4326(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes."
    Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.
    Nucleic Acids Res. 23:2105-2119(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Melibiose permease of Escherichia coli: large scale purification and evidence that H+, Na+, and Li+ sugar symport is catalyzed by a single polypeptide."
    Pourcher T., Leclercq S., Brandolin G., Leblanc G.
    Biochemistry 34:4412-4420(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-8, CHARACTERIZATION, SUBCELLULAR LOCATION, SUBUNIT.
  6. "Membrane topology of the melibiose carrier of Escherichia coli."
    Botfield M.C., Noguchi K., Tsuchiya T., Wilson T.H.
    J. Biol. Chem. 267:1818-1822(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY.
  7. "Membrane topology of the melibiose permease of Escherichia coli studied by melB-phoA fusion analysis."
    Pourcher T., Bibi E., Kaback H.R., Leblanc G.
    Biochemistry 35:4161-4168(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY.
  8. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: K12 / MG1655 / ATCC 47076.
  9. "Melibiose permease of Escherichia coli: mutation of aspartic acid 55 in putative helix II abolishes activation of sugar binding by Na+ ions."
    Pourcher T., Deckert M., Bassilana M., Leblanc G.
    Biochem. Biophys. Res. Commun. 178:1176-1181(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF ASP-59.
  10. "A 3D structure model of the melibiose permease of Escherichia coli represents a distinctive fold for Na+ symporters."
    Yousef M.S., Guan L.
    Proc. Natl. Acad. Sci. U.S.A. 106:15291-15296(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN MODEL.

Entry informationi

Entry nameiMELB_ECOLI
AccessioniPrimary (citable) accession number: P02921
Secondary accession number(s): Q2M6I4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: October 19, 2011
Last modified: July 6, 2016
This is version 140 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.