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Protein

Lactose permease

Gene

lacY

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Responsible for transport of beta-galactosides into the cell, with the concomitant import of a proton (symport system).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei126Substrate binding1
Sitei144Substrate binding1
Sitei269Substrate binding and proton translocation1
Sitei302Proton translocation1
Sitei322Proton translocation1
Sitei325Proton translocation1

GO - Molecular functioni

  • lactose:proton symporter activity Source: CACAO
  • lactose binding Source: EcoliWiki
  • sugar:proton symporter activity Source: EcoliWiki

GO - Biological processi

  • carbohydrate transport Source: CACAO
  • lactose transport Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Sugar transport, Symport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:LACY-MONOMER.
ECOL316407:JW0334-MONOMER.
MetaCyc:LACY-MONOMER.

Protein family/group databases

TCDBi2.A.1.5.1. the major facilitator superfamily (mfs).

Names & Taxonomyi

Protein namesi
Recommended name:
Lactose permease
Alternative name(s):
Lactose-proton symport
Gene namesi
Name:lacY
Ordered Locus Names:b0343, JW0334
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10526. lacY.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 7Cytoplasmic7
Transmembranei8 – 34Helical; Name=1Add BLAST27
Topological domaini35 – 41Periplasmic7
Transmembranei42 – 70Helical; Name=2Add BLAST29
Topological domaini71 – 74Cytoplasmic4
Transmembranei75 – 100Helical; Name=3Add BLAST26
Topological domaini101 – 104Periplasmic4
Transmembranei105 – 129Helical; Name=4Add BLAST25
Topological domaini130 – 140CytoplasmicAdd BLAST11
Transmembranei141 – 163Helical; Name=5Add BLAST23
Topological domaini164 – 166Periplasmic3
Transmembranei167 – 186Helical; Name=6Add BLAST20
Topological domaini187 – 220CytoplasmicAdd BLAST34
Transmembranei221 – 249Helical; Name=7Add BLAST29
Topological domaini250 – 253Periplasmic4
Transmembranei254 – 278Helical; Name=8Add BLAST25
Topological domaini279 – 288Cytoplasmic10
Transmembranei289 – 308Helical; Name=9Add BLAST20
Topological domaini309 – 311Periplasmic3
Transmembranei312 – 334Helical; Name=10Add BLAST23
Topological domaini335 – 346CytoplasmicAdd BLAST12
Transmembranei347 – 374Helical; Name=11Add BLAST28
Topological domaini375 – 377Periplasmic3
Transmembranei378 – 398Helical; Name=12Add BLAST21
Topological domaini399 – 417CytoplasmicAdd BLAST19

GO - Cellular componenti

  • integral component of membrane Source: EcoliWiki
  • integral component of plasma membrane Source: EcoCyc
  • membrane Source: EcoliWiki
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi237D → N or G: Defect in melibiose transport. 1
Mutagenesisi358K → T: Defect in melibiose transport. 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001961841 – 417Lactose permeaseAdd BLAST417

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-formylmethionine; partial1 Publication1

Keywords - PTMi

Formylation

Proteomic databases

PaxDbiP02920.
PRIDEiP02920.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

BioGridi4263185. 7 interactors.
DIPiDIP-10080N.
STRINGi511145.b0343.

Structurei

Secondary structure

1417
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni2 – 4Combined sources3
Helixi7 – 26Combined sources20
Turni27 – 29Combined sources3
Helixi30 – 35Combined sources6
Turni36 – 38Combined sources3
Turni42 – 44Combined sources3
Helixi46 – 67Combined sources22
Helixi68 – 70Combined sources3
Helixi71 – 73Combined sources3
Helixi75 – 82Combined sources8
Helixi84 – 86Combined sources3
Helixi87 – 93Combined sources7
Helixi95 – 100Combined sources6
Helixi105 – 108Combined sources4
Helixi109 – 111Combined sources3
Helixi116 – 119Combined sources4
Helixi122 – 136Combined sources15
Helixi140 – 143Combined sources4
Beta strandi144 – 146Combined sources3
Turni147 – 149Combined sources3
Helixi150 – 164Combined sources15
Helixi167 – 170Combined sources4
Turni171 – 179Combined sources9
Helixi180 – 183Combined sources4
Beta strandi193 – 195Combined sources3
Beta strandi197 – 203Combined sources7
Helixi210 – 216Combined sources7
Helixi220 – 249Combined sources30
Beta strandi251 – 253Combined sources3
Helixi254 – 257Combined sources4
Helixi259 – 286Combined sources28
Helixi288 – 306Combined sources19
Helixi312 – 318Combined sources7
Helixi321 – 340Combined sources20
Helixi343 – 354Combined sources12
Turni355 – 357Combined sources3
Helixi358 – 365Combined sources8
Helixi367 – 370Combined sources4
Helixi372 – 375Combined sources4
Helixi377 – 396Combined sources20
Beta strandi408 – 413Combined sources6
Turni414 – 416Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1M2Umodel-A1-417[»]
1PV6X-ray3.50A/B1-417[»]
1PV7X-ray3.60A/B1-417[»]
2CFPX-ray3.30A1-417[»]
2CFQX-ray2.95A1-417[»]
2V8NX-ray3.60A/B1-417[»]
2Y5YX-ray3.38A/B1-417[»]
4ZYRX-ray3.31A/B1-417[»]
ProteinModelPortaliP02920.
SMRiP02920.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP02920.

Family & Domainsi

Sequence similaritiesi

Belongs to the LacY/RafB permease family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105U0F. Bacteria.
ENOG410XQTD. LUCA.
HOGENOMiHOG000114363.
InParanoidiP02920.
KOiK02532.
OMAiIVGCFKY.
PhylomeDBiP02920.

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiIPR000576. LacY/RafB_perm_fam.
IPR018457. LacY/RafB_perm_fam_CS.
IPR020846. MFS_dom.
[Graphical view]
PfamiPF01306. LacY_symp. 1 hit.
[Graphical view]
PRINTSiPR00174. LACYSMPORT.
SUPFAMiSSF103473. SSF103473. 1 hit.
TIGRFAMsiTIGR00882. 2A0105. 1 hit.
PROSITEiPS00896. LACY_1. 1 hit.
PS00897. LACY_2. 1 hit.
PS50850. MFS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P02920-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYYLKNTNFW MFGLFFFFYF FIMGAYFPFF PIWLHDINHI SKSDTGIIFA
60 70 80 90 100
AISLFSLLFQ PLFGLLSDKL GLRKYLLWII TGMLVMFAPF FIFIFGPLLQ
110 120 130 140 150
YNILVGSIVG GIYLGFCFNA GAPAVEAFIE KVSRRSNFEF GRARMFGCVG
160 170 180 190 200
WALCASIVGI MFTINNQFVF WLGSGCALIL AVLLFFAKTD APSSATVANA
210 220 230 240 250
VGANHSAFSL KLALELFRQP KLWFLSLYVI GVSCTYDVFD QQFANFFTSF
260 270 280 290 300
FATGEQGTRV FGYVTTMGEL LNASIMFFAP LIINRIGGKN ALLLAGTIMS
310 320 330 340 350
VRIIGSSFAT SALEVVILKT LHMFEVPFLL VGCFKYITSQ FEVRFSATIY
360 370 380 390 400
LVCFCFFKQL AMIFMSVLAG NMYESIGFQG AYLVLGLVAL GFTLISVFTL
410
SGPGPLSLLR RQVNEVA
Length:417
Mass (Da):46,503
Last modified:July 21, 1986 - v1
Checksum:i24A8062F628CDA32
GO

Mass spectrometryi

Molecular mass is 47357 Da from positions 1 - 417. Determined by ESI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01636 Genomic DNA. Translation: AAA24054.1.
V00295 Genomic DNA. Translation: CAA23571.1.
X56095 Genomic DNA. Translation: CAA39575.1.
U73857 Genomic DNA. Translation: AAB18067.1.
U00096 Genomic DNA. Translation: AAC73446.1.
AP009048 Genomic DNA. Translation: BAE76125.1.
PIRiA03418. GREC.
RefSeqiNP_414877.1. NC_000913.3.
WP_000291549.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73446; AAC73446; b0343.
BAE76125; BAE76125; BAE76125.
GeneIDi949083.
KEGGiecj:JW0334.
eco:b0343.
PATRICi32115819. VBIEscCol129921_0351.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01636 Genomic DNA. Translation: AAA24054.1.
V00295 Genomic DNA. Translation: CAA23571.1.
X56095 Genomic DNA. Translation: CAA39575.1.
U73857 Genomic DNA. Translation: AAB18067.1.
U00096 Genomic DNA. Translation: AAC73446.1.
AP009048 Genomic DNA. Translation: BAE76125.1.
PIRiA03418. GREC.
RefSeqiNP_414877.1. NC_000913.3.
WP_000291549.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1M2Umodel-A1-417[»]
1PV6X-ray3.50A/B1-417[»]
1PV7X-ray3.60A/B1-417[»]
2CFPX-ray3.30A1-417[»]
2CFQX-ray2.95A1-417[»]
2V8NX-ray3.60A/B1-417[»]
2Y5YX-ray3.38A/B1-417[»]
4ZYRX-ray3.31A/B1-417[»]
ProteinModelPortaliP02920.
SMRiP02920.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263185. 7 interactors.
DIPiDIP-10080N.
STRINGi511145.b0343.

Protein family/group databases

TCDBi2.A.1.5.1. the major facilitator superfamily (mfs).

Proteomic databases

PaxDbiP02920.
PRIDEiP02920.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73446; AAC73446; b0343.
BAE76125; BAE76125; BAE76125.
GeneIDi949083.
KEGGiecj:JW0334.
eco:b0343.
PATRICi32115819. VBIEscCol129921_0351.

Organism-specific databases

EchoBASEiEB0521.
EcoGeneiEG10526. lacY.

Phylogenomic databases

eggNOGiENOG4105U0F. Bacteria.
ENOG410XQTD. LUCA.
HOGENOMiHOG000114363.
InParanoidiP02920.
KOiK02532.
OMAiIVGCFKY.
PhylomeDBiP02920.

Enzyme and pathway databases

BioCyciEcoCyc:LACY-MONOMER.
ECOL316407:JW0334-MONOMER.
MetaCyc:LACY-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP02920.
PROiP02920.

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiIPR000576. LacY/RafB_perm_fam.
IPR018457. LacY/RafB_perm_fam_CS.
IPR020846. MFS_dom.
[Graphical view]
PfamiPF01306. LacY_symp. 1 hit.
[Graphical view]
PRINTSiPR00174. LACYSMPORT.
SUPFAMiSSF103473. SSF103473. 1 hit.
TIGRFAMsiTIGR00882. 2A0105. 1 hit.
PROSITEiPS00896. LACY_1. 1 hit.
PS00897. LACY_2. 1 hit.
PS50850. MFS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLACY_ECOLI
AccessioniPrimary (citable) accession number: P02920
Secondary accession number(s): Q2MC81
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: November 2, 2016
This is version 154 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.